Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices.
Identifieur interne : 000929 ( PubMed/Curation ); précédent : 000928; suivant : 000930Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices.
Auteurs : Montserrat Cortina-Puig [France] ; Xavier Mu Oz-Berbel ; Regis Rouillon ; Carole Calas-Blanchard ; Jean-Louis MartySource :
- Bioelectrochemistry (Amsterdam, Netherlands) [ 1878-562X ] ; 2009.
English descriptors
- KwdEn :
- Animals, Antioxidants (analysis), Antioxidants (chemistry), Antioxidants (metabolism), Beverages, Biosensing Techniques (methods), Cattle, Citrus sinensis (chemistry), Cytochromes c (chemistry), Cytochromes c (metabolism), Electrochemistry, Electrodes, Enzymes, Immobilized (chemistry), Enzymes, Immobilized (metabolism), Gold (chemistry), Gold (metabolism), Horses, Printing, Superoxides (chemistry), Superoxides (metabolism), Xanthine Oxidase (chemistry), Xanthine Oxidase (metabolism).
- MESH :
- chemical , analysis : Antioxidants.
- chemical , chemistry : Antioxidants, Cytochromes c, Enzymes, Immobilized, Gold, Superoxides, Xanthine Oxidase.
- chemical , metabolism : Antioxidants, Cytochromes c, Enzymes, Immobilized, Gold, Superoxides, Xanthine Oxidase.
- chemistry : Citrus sinensis.
- methods : Biosensing Techniques.
- Animals, Beverages, Cattle, Electrochemistry, Electrodes, Horses, Printing.
Abstract
This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.
DOI: 10.1016/j.bioelechem.2009.04.004
PubMed: 19447685
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pubmed:19447685Le document en format XML
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<author><name sortKey="Calas Blanchard, Carole" sort="Calas Blanchard, Carole" uniqKey="Calas Blanchard C" first="Carole" last="Calas-Blanchard">Carole Calas-Blanchard</name>
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<front><div type="abstract" xml:lang="en">This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.</div>
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