Development of a new high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry method for the determination of low molecular mass organic acids in plant tissue extracts.
Identifieur interne : 000723 ( PubMed/Curation ); précédent : 000722; suivant : 000724Development of a new high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry method for the determination of low molecular mass organic acids in plant tissue extracts.
Auteurs : Ruben Rellan-Alvarez [Espagne] ; Sara Lopez-Gomollon ; Javier Abadia ; Ana Alvarez-FernandezSource :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2011.
English descriptors
- KwdEn :
- Beta vulgaris (chemistry), Beverages (analysis), Carbon Isotopes, Carboxylic Acids (analysis), Carboxylic Acids (chemistry), Chromatography, High Pressure Liquid (methods), Citrus sinensis (chemistry), Fruit (chemistry), Lycopersicon esculentum (chemistry), Molecular Weight, Plant Extracts (chemistry), Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization (methods).
- MESH :
- chemical , analysis : Carboxylic Acids.
- chemical , chemistry : Carboxylic Acids, Plant Extracts.
- chemical : Carbon Isotopes.
- analysis : Beverages.
- chemistry : Beta vulgaris, Citrus sinensis, Fruit, Lycopersicon esculentum.
- methods : Chromatography, High Pressure Liquid, Spectrometry, Mass, Electrospray Ionization.
- Molecular Weight, Reproducibility of Results.
Abstract
A liquid chromatography-electrospray ionization time-of-flight mass spectrometry method has been developed for the direct and simultaneous determination of 10 low molecular mass organic acids in different plant tissue extracts. The method does not include a derivatization step. Quantification was accomplished using (13)C-labeled malic and succinic acids as internal standards. Good limits of detection (0.05-1.27 pmol) were obtained for malic, 2-oxoglutaric, succinic, quinic, shikimic, cis-aconitic, and citric acids, whereas for oxalic, ascorbic, and fumaric acids limits of detection were 255, 25, and 11 pmol, respectively. Repeatability values for the retention time and peak area were <5%, with the exception of ascorbic acid. Analyte recovery was between 92% and 110% in most cases, with the exception of oxalic (39-108%), 2-oxoglutaric (44-69%), and ascorbic (22-86%) acids, which may require specific extraction procedures and use of the corresponding (13)C-labeled organic acid as internal standards to improve accuracy. The method was applied to three types of plant materials: sugar beet leaf extracts, tomato xylem sap, and commercial orange juice.
DOI: 10.1021/jf200482a
PubMed: 21619056
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last="Lopez-Gomollon">Sara Lopez-Gomollon</name></author><author><name sortKey="Abadia, Javier" sort="Abadia, Javier" uniqKey="Abadia J" first="Javier" last="Abadia">Javier Abadia</name></author><author><name sortKey="Alvarez Fernandez, Ana" sort="Alvarez Fernandez, Ana" uniqKey="Alvarez Fernandez A" first="Ana" last="Alvarez-Fernandez">Ana Alvarez-Fernandez</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21619056</idno><idno type="pmid">21619056</idno><idno type="doi">10.1021/jf200482a</idno><idno type="wicri:Area/PubMed/Corpus">000723</idno><idno type="wicri:Area/PubMed/Curation">000723</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of a new high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry method for the determination of low molecular mass organic acids in plant tissue extracts.</title><author><name sortKey="Rellan Alvarez, Ruben" sort="Rellan Alvarez, Ruben" uniqKey="Rellan Alvarez R" first="Ruben" last="Rellan-Alvarez">Ruben Rellan-Alvarez</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Nutrition, Aula Dei Experimental Station, Consejo Superior de Investigaciones Científicas, Zaragoza, Spain. ana.alvarez@eead.csic.es</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Nutrition, Aula Dei Experimental Station, Consejo Superior de Investigaciones Científicas, Zaragoza</wicri:regionArea></affiliation></author><author><name sortKey="Lopez Gomollon, Sara" sort="Lopez Gomollon, Sara" uniqKey="Lopez Gomollon S" first="Sara" last="Lopez-Gomollon">Sara Lopez-Gomollon</name></author><author><name sortKey="Abadia, Javier" sort="Abadia, Javier" uniqKey="Abadia J" first="Javier" last="Abadia">Javier Abadia</name></author><author><name sortKey="Alvarez Fernandez, Ana" sort="Alvarez Fernandez, Ana" uniqKey="Alvarez Fernandez A" first="Ana" last="Alvarez-Fernandez">Ana Alvarez-Fernandez</name></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Beta vulgaris (chemistry)</term><term>Beverages (analysis)</term><term>Carbon Isotopes</term><term>Carboxylic Acids (analysis)</term><term>Carboxylic Acids (chemistry)</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Citrus sinensis (chemistry)</term><term>Fruit (chemistry)</term><term>Lycopersicon esculentum (chemistry)</term><term>Molecular Weight</term><term>Plant Extracts (chemistry)</term><term>Reproducibility of Results</term><term>Spectrometry, Mass, Electrospray Ionization (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carboxylic Acids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carboxylic Acids</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbon Isotopes</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Beta vulgaris</term><term>Citrus sinensis</term><term>Fruit</term><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Weight</term><term>Reproducibility of Results</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A liquid chromatography-electrospray ionization time-of-flight mass spectrometry method has been developed for the direct and simultaneous determination of 10 low molecular mass organic acids in different plant tissue extracts. The method does not include a derivatization step. Quantification was accomplished using (13)C-labeled malic and succinic acids as internal standards. Good limits of detection (0.05-1.27 pmol) were obtained for malic, 2-oxoglutaric, succinic, quinic, shikimic, cis-aconitic, and citric acids, whereas for oxalic, ascorbic, and fumaric acids limits of detection were 255, 25, and 11 pmol, respectively. Repeatability values for the retention time and peak area were <5%, with the exception of ascorbic acid. Analyte recovery was between 92% and 110% in most cases, with the exception of oxalic (39-108%), 2-oxoglutaric (44-69%), and ascorbic (22-86%) acids, which may require specific extraction procedures and use of the corresponding (13)C-labeled organic acid as internal standards to improve accuracy. The method was applied to three types of plant materials: sugar beet leaf extracts, tomato xylem sap, and commercial orange juice.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21619056</PMID><DateCreated><Year>2011</Year><Month>7</Month><Day>6</Day></DateCreated><DateCompleted><Year>2011</Year><Month>10</Month><Day>28</Day></DateCompleted><DateRevised><Year>2011</Year><Month>7</Month><Day>6</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>59</Volume><Issue>13</Issue><PubDate><Year>2011</Year><Month>Jul</Month><Day>13</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J. Agric. Food Chem.</ISOAbbreviation></Journal><ArticleTitle>Development of a new high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry method for the determination of low molecular mass organic acids in plant tissue extracts.</ArticleTitle><Pagination><MedlinePgn>6864-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/jf200482a</ELocationID><Abstract><AbstractText>A liquid chromatography-electrospray ionization time-of-flight mass spectrometry method has been developed for the direct and simultaneous determination of 10 low molecular mass organic acids in different plant tissue extracts. The method does not include a derivatization step. Quantification was accomplished using (13)C-labeled malic and succinic acids as internal standards. Good limits of detection (0.05-1.27 pmol) were obtained for malic, 2-oxoglutaric, succinic, quinic, shikimic, cis-aconitic, and citric acids, whereas for oxalic, ascorbic, and fumaric acids limits of detection were 255, 25, and 11 pmol, respectively. Repeatability values for the retention time and peak area were <5%, with the exception of ascorbic acid. Analyte recovery was between 92% and 110% in most cases, with the exception of oxalic (39-108%), 2-oxoglutaric (44-69%), and ascorbic (22-86%) acids, which may require specific extraction procedures and use of the corresponding (13)C-labeled organic acid as internal standards to improve accuracy. 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