Bacterial detection using unlabeled phage amplification and mass spectrometry through structural and nonstructural phage markers.
Identifieur interne : 000357 ( PubMed/Curation ); précédent : 000356; suivant : 000358Bacterial detection using unlabeled phage amplification and mass spectrometry through structural and nonstructural phage markers.
Auteurs : Armelle Martelet [France] ; Guillaume L'Hostis ; Paulo Tavares ; Sandrine Brasilès ; François Fenaille ; Christine Rozand ; Alain Theretz ; Gaspard Gervasi ; Jean-Claude Tabet ; Eric Ezan ; Christophe Junot ; Bruno H. Muller ; François BecherSource :
- Journal of proteome research [ 1535-3907 ] ; 2014.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Bacillus subtilis (isolation & purification), Bacillus subtilis (virology), Beverages (analysis), Beverages (microbiology), Citrus sinensis, Coliphages (genetics), Escherichia coli (isolation & purification), Escherichia coli (virology), Food Analysis, Humans, Lysogeny, Meat Products (analysis), Meat Products (microbiology), Molecular Sequence Data, Peptide Library, Spectrometry, Mass, Electrospray Ionization, Swine, Viral Proteins (genetics).
- MESH :
- chemical , genetics : Viral Proteins.
- chemical : Peptide Library.
- analysis : Beverages, Meat Products.
- genetics : Coliphages.
- isolation & purification : Bacillus subtilis, Escherichia coli.
- microbiology : Beverages, Meat Products.
- virology : Bacillus subtilis, Escherichia coli.
- Amino Acid Sequence, Animals, Citrus sinensis, Food Analysis, Humans, Lysogeny, Molecular Sequence Data, Spectrometry, Mass, Electrospray Ionization, Swine.
Abstract
According to the World Health Organization, food safety is an essential public health priority. In this context, we report a relevant proof of feasibility for the indirect specific detection of bacteria in food samples using unlabeled phage amplification coupled to ESI mass spectrometry analysis and illustrated with the model phage systems T4 and SPP1. High-resolving power mass spectrometry analysis (including bottom-up and top-down protein analysis) was used for the discovery of specific markers of phage infection. Structural components of the viral particle and nonstructural proteins encoded by the phage genome were identified. Then, targeted detection of these markers was performed on a triple quadrupole mass spectrometer operating in the selected reaction monitoring mode. E. coli at 1 × 10(5), 5 × 10(5), and 1 × 10(6) CFU/mL concentrations was successfully detected after only a 2 h infection time by monitoring phage T4 structural markers in Luria-Bertani broth, orange juice, and French bean stew ("cassoulet") matrices. Reproducible detection of nonstructural markers was also demonstrated, particularly when a high titer of input phages was required to achieve successful amplification. This strategy provides a highly time-effective and sensitive assay for bacterial detection.
DOI: 10.1021/pr400991t
PubMed: 24517284
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Muller</name></author><author><name sortKey="Becher, Francois" sort="Becher, Francois" uniqKey="Becher F" first="François" last="Becher">François Becher</name></author></analytic><series><title level="j">Journal of proteome research</title><idno type="eISSN">1535-3907</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Bacillus subtilis (isolation & purification)</term><term>Bacillus subtilis (virology)</term><term>Beverages (analysis)</term><term>Beverages (microbiology)</term><term>Citrus sinensis</term><term>Coliphages (genetics)</term><term>Escherichia coli (isolation & purification)</term><term>Escherichia coli (virology)</term><term>Food Analysis</term><term>Humans</term><term>Lysogeny</term><term>Meat Products (analysis)</term><term>Meat Products (microbiology)</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Swine</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Peptide Library</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term><term>Meat Products</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coliphages</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacillus subtilis</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Beverages</term><term>Meat Products</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Bacillus subtilis</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Citrus sinensis</term><term>Food Analysis</term><term>Humans</term><term>Lysogeny</term><term>Molecular Sequence Data</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Swine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">According to the World Health Organization, food safety is an essential public health priority. In this context, we report a relevant proof of feasibility for the indirect specific detection of bacteria in food samples using unlabeled phage amplification coupled to ESI mass spectrometry analysis and illustrated with the model phage systems T4 and SPP1. High-resolving power mass spectrometry analysis (including bottom-up and top-down protein analysis) was used for the discovery of specific markers of phage infection. Structural components of the viral particle and nonstructural proteins encoded by the phage genome were identified. Then, targeted detection of these markers was performed on a triple quadrupole mass spectrometer operating in the selected reaction monitoring mode. E. coli at 1 × 10(5), 5 × 10(5), and 1 × 10(6) CFU/mL concentrations was successfully detected after only a 2 h infection time by monitoring phage T4 structural markers in Luria-Bertani broth, orange juice, and French bean stew ("cassoulet") matrices. Reproducible detection of nonstructural markers was also demonstrated, particularly when a high titer of input phages was required to achieve successful amplification. This strategy provides a highly time-effective and sensitive assay for bacterial detection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24517284</PMID><DateCreated><Year>2014</Year><Month>3</Month><Day>7</Day></DateCreated><DateCompleted><Year>2014</Year><Month>11</Month><Day>03</Day></DateCompleted><DateRevised><Year>2014</Year><Month>3</Month><Day>7</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1535-3907</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Mar</Month><Day>7</Day></PubDate></JournalIssue><Title>Journal of proteome research</Title><ISOAbbreviation>J. Proteome Res.</ISOAbbreviation></Journal><ArticleTitle>Bacterial detection using unlabeled phage amplification and mass spectrometry through structural and nonstructural phage markers.</ArticleTitle><Pagination><MedlinePgn>1450-65</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/pr400991t</ELocationID><Abstract><AbstractText>According to the World Health Organization, food safety is an essential public health priority. In this context, we report a relevant proof of feasibility for the indirect specific detection of bacteria in food samples using unlabeled phage amplification coupled to ESI mass spectrometry analysis and illustrated with the model phage systems T4 and SPP1. High-resolving power mass spectrometry analysis (including bottom-up and top-down protein analysis) was used for the discovery of specific markers of phage infection. Structural components of the viral particle and nonstructural proteins encoded by the phage genome were identified. Then, targeted detection of these markers was performed on a triple quadrupole mass spectrometer operating in the selected reaction monitoring mode. E. coli at 1 × 10(5), 5 × 10(5), and 1 × 10(6) CFU/mL concentrations was successfully detected after only a 2 h infection time by monitoring phage T4 structural markers in Luria-Bertani broth, orange juice, and French bean stew ("cassoulet") matrices. Reproducible detection of nonstructural markers was also demonstrated, particularly when a high titer of input phages was required to achieve successful amplification. This strategy provides a highly time-effective and sensitive assay for bacterial detection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Martelet</LastName><ForeName>Armelle</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>bioMérieux S.A. , 376, Chemin de l'Orme, 69280 Marcy-l'Etoile, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>L'Hostis</LastName><ForeName>Guillaume</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Tavares</LastName><ForeName>Paulo</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Brasilès</LastName><ForeName>Sandrine</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Fenaille</LastName><ForeName>François</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Rozand</LastName><ForeName>Christine</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Theretz</LastName><ForeName>Alain</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Gervasi</LastName><ForeName>Gaspard</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Tabet</LastName><ForeName>Jean-Claude</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Ezan</LastName><ForeName>Eric</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Junot</LastName><ForeName>Christophe</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Muller</LastName><ForeName>Bruno H</ForeName><Initials>BH</Initials></Author><Author ValidYN="Y"><LastName>Becher</LastName><ForeName>François</ForeName><Initials>F</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>Feb</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Proteome Res</MedlineTA><NlmUniqueID>101128775</NlmUniqueID><ISSNLinking>1535-3893</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019151">Peptide Library</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001412" MajorTopicYN="N">Bacillus subtilis</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001628" MajorTopicYN="N">Beverages</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="Y">Citrus sinensis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003090" MajorTopicYN="N">Coliphages</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005504" MajorTopicYN="N">Food Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008242" MajorTopicYN="N">Lysogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008461" MajorTopicYN="N">Meat Products</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019151" MajorTopicYN="N">Peptide Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013552" MajorTopicYN="N">Swine</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24517284</ArticleId><ArticleId IdType="doi">10.1021/pr400991t</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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