Microflow liquid chromatography coupled to mass spectrometry--an approach to significantly increase sensitivity, decrease matrix effects, and reduce organic solvent usage in pesticide residue analysis.
Identifieur interne : 000234 ( PubMed/Curation ); précédent : 000233; suivant : 000235Microflow liquid chromatography coupled to mass spectrometry--an approach to significantly increase sensitivity, decrease matrix effects, and reduce organic solvent usage in pesticide residue analysis.
Auteurs : Ana Uclés Moreno [Espagne] ; Sonia Herrera L Pez ; Barbara Reichert ; Ana Lozano Fernández ; María Dolores Hernando Guil ; Amadeo Rodríguez Fernández-AlbaSource :
- Analytical chemistry [ 1520-6882 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Pesticide Residues.
- chemistry : Capsicum, Citrus sinensis, Lycopersicon esculentum, Solvents.
- methods : Chromatography, Liquid, Solid Phase Extraction, Spectrometry, Mass, Electrospray Ionization.
- Sensitivity and Specificity.
Abstract
This manuscript reports a new pesticide residue analysis method employing a microflow-liquid chromatography system coupled to a triple quadrupole mass spectrometer (microflow-LC-ESI-QqQ-MS). This uses an electrospray ionization source with a narrow tip emitter to generate smaller droplets. A validation study was undertaken to establish performance characteristics for this new approach on 90 pesticide residues, including their degradation products, in three commodities (tomato, pepper, and orange). The significant benefits of the microflow-LC-MS/MS-based method were a high sensitivity gain and a notable reduction in matrix effects delivered by a dilution of the sample (up to 30-fold); this is as a result of competition reduction between the matrix compounds and analytes for charge during ionization. Overall robustness and a capability to withstand long analytical runs using the microflow-LC-MS system have been demonstrated (for 100 consecutive injections without any maintenance being required). Quality controls based on the results of internal standards added at the samples' extraction, dilution, and injection steps were also satisfactory. The LOQ values were mostly 5 μg kg(-1) for almost all pesticide residues. Other benefits were a substantial reduction in solvent usage and waste disposal as well as a decrease in the run-time. The method was successfully applied in the routine analysis of 50 fruit and vegetable samples labeled as organically produced.
DOI: 10.1021/ac5035852
PubMed: 25495653
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Sacramento, s/n, 04120 La Cañada de San Urbano, Almerı́a</wicri:regionArea></affiliation></author><author><name sortKey="Herrera L Pez, Sonia" sort="Herrera L Pez, Sonia" uniqKey="Herrera L Pez S" first="Sonia" last="Herrera L Pez">Sonia Herrera L Pez</name></author><author><name sortKey="Reichert, Barbara" sort="Reichert, Barbara" uniqKey="Reichert B" first="Barbara" last="Reichert">Barbara Reichert</name></author><author><name sortKey="Lozano Fernandez, Ana" sort="Lozano Fernandez, Ana" uniqKey="Lozano Fernandez A" first="Ana" last="Lozano Fernández">Ana Lozano Fernández</name></author><author><name sortKey="Hernando Guil, Maria Dolores" sort="Hernando Guil, Maria Dolores" uniqKey="Hernando Guil M" first="María Dolores" last="Hernando Guil">María Dolores Hernando Guil</name></author><author><name sortKey="Fernandez Alba, Amadeo Rodriguez" sort="Fernandez Alba, Amadeo Rodriguez" uniqKey="Fernandez Alba A" first="Amadeo Rodríguez" last="Fernández-Alba">Amadeo Rodríguez Fernández-Alba</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25495653</idno><idno type="pmid">25495653</idno><idno type="doi">10.1021/ac5035852</idno><idno type="wicri:Area/PubMed/Corpus">000234</idno><idno type="wicri:Area/PubMed/Curation">000234</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microflow liquid chromatography coupled to mass spectrometry--an approach to significantly increase sensitivity, decrease matrix effects, and reduce organic solvent usage in pesticide residue analysis.</title><author><name sortKey="Ucles Moreno, Ana" sort="Ucles Moreno, Ana" uniqKey="Ucles Moreno A" first="Ana" last="Uclés Moreno">Ana Uclés Moreno</name><affiliation wicri:level="1"><nlm:affiliation>Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, University of Almeria , Ctra. Sacramento, s/n, 04120 La Cañada de San Urbano, Almerı́a, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, University of Almeria , Ctra. 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The LOQ values were mostly 5 μg kg(-1) for almost all pesticide residues. Other benefits were a substantial reduction in solvent usage and waste disposal as well as a decrease in the run-time. The method was successfully applied in the routine analysis of 50 fruit and vegetable samples labeled as organically produced.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25495653</PMID><DateCreated><Year>2015</Year><Month>1</Month><Day>21</Day></DateCreated><DateCompleted><Year>2015</Year><Month>09</Month><Day>14</Day></DateCompleted><DateRevised><Year>2015</Year><Month>1</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6882</ISSN><JournalIssue CitedMedium="Internet"><Volume>87</Volume><Issue>2</Issue><PubDate><Year>2015</Year><Month>Jan</Month><Day>20</Day></PubDate></JournalIssue><Title>Analytical chemistry</Title><ISOAbbreviation>Anal. Chem.</ISOAbbreviation></Journal><ArticleTitle>Microflow liquid chromatography coupled to mass spectrometry--an approach to significantly increase sensitivity, decrease matrix effects, and reduce organic solvent usage in pesticide residue analysis.</ArticleTitle><Pagination><MedlinePgn>1018-25</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/ac5035852</ELocationID><Abstract><AbstractText>This manuscript reports a new pesticide residue analysis method employing a microflow-liquid chromatography system coupled to a triple quadrupole mass spectrometer (microflow-LC-ESI-QqQ-MS). This uses an electrospray ionization source with a narrow tip emitter to generate smaller droplets. A validation study was undertaken to establish performance characteristics for this new approach on 90 pesticide residues, including their degradation products, in three commodities (tomato, pepper, and orange). The significant benefits of the microflow-LC-MS/MS-based method were a high sensitivity gain and a notable reduction in matrix effects delivered by a dilution of the sample (up to 30-fold); this is as a result of competition reduction between the matrix compounds and analytes for charge during ionization. Overall robustness and a capability to withstand long analytical runs using the microflow-LC-MS system have been demonstrated (for 100 consecutive injections without any maintenance being required). Quality controls based on the results of internal standards added at the samples' extraction, dilution, and injection steps were also satisfactory. The LOQ values were mostly 5 μg kg(-1) for almost all pesticide residues. Other benefits were a substantial reduction in solvent usage and waste disposal as well as a decrease in the run-time. The method was successfully applied in the routine analysis of 50 fruit and vegetable samples labeled as organically produced.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Uclés Moreno</LastName><ForeName>Ana</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, University of Almeria , Ctra. Sacramento, s/n, 04120 La Cañada de San Urbano, Almerı́a, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Herrera López</LastName><ForeName>Sonia</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Reichert</LastName><ForeName>Barbara</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Lozano Fernández</LastName><ForeName>Ana</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Hernando Guil</LastName><ForeName>María Dolores</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Fernández-Alba</LastName><ForeName>Amadeo Rodríguez</ForeName><Initials>AR</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>Dec</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Chem</MedlineTA><NlmUniqueID>0370536</NlmUniqueID><ISSNLinking>0003-2700</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010573">Pesticide Residues</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012997">Solvents</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002212" MajorTopicYN="N">Capsicum</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002853" MajorTopicYN="N">Chromatography, Liquid</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010573" MajorTopicYN="N">Pesticide Residues</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052616" MajorTopicYN="N">Solid Phase Extraction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012997" MajorTopicYN="N">Solvents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25495653</ArticleId><ArticleId IdType="doi">10.1021/ac5035852</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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