Application of Microwave Irradiation and Heat to Improve Gliadin Detection and Ricin ELISA Throughput with Food Samples.
Identifieur interne : 000166 ( PubMed/Curation ); précédent : 000165; suivant : 000167Application of Microwave Irradiation and Heat to Improve Gliadin Detection and Ricin ELISA Throughput with Food Samples.
Auteurs : Eric A E. Garber [États-Unis] ; Joseph Thole [États-Unis]Source :
- Toxins [ 2072-6651 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Gliadin, Ricin.
- analysis : Bread, Carbonated Beverages, Food Contamination, Fruit and Vegetable Juices.
- chemistry : Milk.
- methods : Enzyme-Linked Immunosorbent Assay.
- Animals, Citrus sinensis, Hot Temperature, Microwaves, Toxins, Biological.
Abstract
The utility of microwave irradiation to accelerate the onset of equilibrium and improve ELISA performance was examined using ELISAs for the detection of the plant toxin ricin and gliadin. The ricin ELISA normally requires several one hour incubations at 37 °C, a total assay time of approximately five hours, and employs a complex buffer containing PBS, Tween-20®, and non-fat milk. Different energy levels and pulse designs were compared to the use of abbreviated incubation times at 37 °C for the detection of ricin in food. The use of microwave irradiation had no significant advantage over the application of heat using an oven incubator and performed worse with some foods. In contrast, a gliadin ELISA that relied on 30 min incubation steps at room temperature and a salt-based buffer performed better upon irradiation but also displayed improvement upon incubating the microtiter plate at 37 °C. Whether microwave irradiation was advantageous compared to incubation in an oven was inconclusive. However, by abbreviating the incubation time of the ricin ELISA, it was possible to cut the assay time to less than 2 hours and still display LOD values < 10 ppb and recoveries of 78%-98%.
DOI: 10.3390/toxins7062135
PubMed: 26110503
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Garber</name><affiliation wicri:level="1"><nlm:affiliation>Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USA. eric.garber@fda.hhs.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740</wicri:regionArea></affiliation></author><author><name sortKey="Thole, Joseph" sort="Thole, Joseph" uniqKey="Thole J" first="Joseph" last="Thole">Joseph Thole</name><affiliation wicri:level="1"><nlm:affiliation>Joint Institute for Food Safety and Applied Nutrition (JIFSAN), University of Maryland, College Park, MD 20742, USA. jthole@terpmail.umd.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Joint Institute for Food Safety and Applied Nutrition (JIFSAN), University of Maryland, College Park, MD 20742</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26110503</idno><idno type="pmid">26110503</idno><idno type="doi">10.3390/toxins7062135</idno><idno type="wicri:Area/PubMed/Corpus">000166</idno><idno type="wicri:Area/PubMed/Curation">000166</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Application of Microwave Irradiation and Heat to Improve Gliadin Detection and Ricin ELISA Throughput with Food Samples.</title><author><name sortKey="Garber, Eric A E" sort="Garber, Eric A E" uniqKey="Garber E" first="Eric A E" last="Garber">Eric A E. Garber</name><affiliation wicri:level="1"><nlm:affiliation>Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USA. eric.garber@fda.hhs.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740</wicri:regionArea></affiliation></author><author><name sortKey="Thole, Joseph" sort="Thole, Joseph" uniqKey="Thole J" first="Joseph" last="Thole">Joseph Thole</name><affiliation wicri:level="1"><nlm:affiliation>Joint Institute for Food Safety and Applied Nutrition (JIFSAN), University of Maryland, College Park, MD 20742, USA. jthole@terpmail.umd.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Joint Institute for Food Safety and Applied Nutrition (JIFSAN), University of Maryland, College Park, MD 20742</wicri:regionArea></affiliation></author></analytic><series><title level="j">Toxins</title><idno type="eISSN">2072-6651</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bread (analysis)</term><term>Carbonated Beverages (analysis)</term><term>Citrus sinensis</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Food Contamination (analysis)</term><term>Fruit and Vegetable Juices (analysis)</term><term>Gliadin (analysis)</term><term>Hot Temperature</term><term>Microwaves</term><term>Milk (chemistry)</term><term>Ricin (analysis)</term><term>Toxins, Biological</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Gliadin</term><term>Ricin</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Bread</term><term>Carbonated Beverages</term><term>Food Contamination</term><term>Fruit and Vegetable Juices</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Milk</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Citrus sinensis</term><term>Hot Temperature</term><term>Microwaves</term><term>Toxins, Biological</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The utility of microwave irradiation to accelerate the onset of equilibrium and improve ELISA performance was examined using ELISAs for the detection of the plant toxin ricin and gliadin. The ricin ELISA normally requires several one hour incubations at 37 °C, a total assay time of approximately five hours, and employs a complex buffer containing PBS, Tween-20®, and non-fat milk. Different energy levels and pulse designs were compared to the use of abbreviated incubation times at 37 °C for the detection of ricin in food. The use of microwave irradiation had no significant advantage over the application of heat using an oven incubator and performed worse with some foods. In contrast, a gliadin ELISA that relied on 30 min incubation steps at room temperature and a salt-based buffer performed better upon irradiation but also displayed improvement upon incubating the microtiter plate at 37 °C. Whether microwave irradiation was advantageous compared to incubation in an oven was inconclusive. However, by abbreviating the incubation time of the ricin ELISA, it was possible to cut the assay time to less than 2 hours and still display LOD values < 10 ppb and recoveries of 78%-98%.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26110503</PMID><DateCreated><Year>2015</Year><Month>6</Month><Day>26</Day></DateCreated><DateCompleted><Year>2016</Year><Month>03</Month><Day>31</Day></DateCompleted><DateRevised><Year>2015</Year><Month>7</Month><Day>7</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2072-6651</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>6</Issue><PubDate><Year>2015</Year><Month>Jun</Month><Day>11</Day></PubDate></JournalIssue><Title>Toxins</Title><ISOAbbreviation>Toxins (Basel)</ISOAbbreviation></Journal><ArticleTitle>Application of Microwave Irradiation and Heat to Improve Gliadin Detection and Ricin ELISA Throughput with Food Samples.</ArticleTitle><Pagination><MedlinePgn>2135-44</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3390/toxins7062135</ELocationID><Abstract><AbstractText>The utility of microwave irradiation to accelerate the onset of equilibrium and improve ELISA performance was examined using ELISAs for the detection of the plant toxin ricin and gliadin. The ricin ELISA normally requires several one hour incubations at 37 °C, a total assay time of approximately five hours, and employs a complex buffer containing PBS, Tween-20®, and non-fat milk. Different energy levels and pulse designs were compared to the use of abbreviated incubation times at 37 °C for the detection of ricin in food. The use of microwave irradiation had no significant advantage over the application of heat using an oven incubator and performed worse with some foods. In contrast, a gliadin ELISA that relied on 30 min incubation steps at room temperature and a salt-based buffer performed better upon irradiation but also displayed improvement upon incubating the microtiter plate at 37 °C. Whether microwave irradiation was advantageous compared to incubation in an oven was inconclusive. However, by abbreviating the incubation time of the ricin ELISA, it was possible to cut the assay time to less than 2 hours and still display LOD values < 10 ppb and recoveries of 78%-98%.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Garber</LastName><ForeName>Eric A E</ForeName><Initials>EA</Initials><AffiliationInfo><Affiliation>Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USA. eric.garber@fda.hhs.gov.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thole</LastName><ForeName>Joseph</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Joint Institute for Food Safety and Applied Nutrition (JIFSAN), University of Maryland, College Park, MD 20742, USA. jthole@terpmail.umd.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>Jun</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Toxins (Basel)</MedlineTA><NlmUniqueID>101530765</NlmUniqueID><ISSNLinking>2072-6651</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014118">Toxins, Biological</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-90-3</RegistryNumber><NameOfSubstance UI="D005903">Gliadin</NameOfSubstance></Chemical><Chemical><RegistryNumber>9009-86-3</RegistryNumber><NameOfSubstance UI="D012276">Ricin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):115-6</RefSource><PMID Version="1">12563796</PMID></CommentsCorrections><CommentsCorrections 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Version="1">24320219</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Immunol Methods. 2008 Jul 31;336(2):251-4</RefSource><PMID Version="1">18561943</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Bioelectromagnetics. 2000 Jan;21(1):68-72</RefSource><PMID Version="1">10615094</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Histochem J. 1988 Jun-Jul;20(6-7):388-96</RefSource><PMID Version="1">3065306</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001939" MajorTopicYN="N">Bread</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002253" MajorTopicYN="N">Carbonated Beverages</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005506" MajorTopicYN="N">Food Contamination</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000067030" MajorTopicYN="N">Fruit and Vegetable Juices</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005903" MajorTopicYN="N">Gliadin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006358" MajorTopicYN="N">Hot Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008872" MajorTopicYN="N">Microwaves</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008892" MajorTopicYN="N">Milk</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012276" MajorTopicYN="N">Ricin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014118" MajorTopicYN="N">Toxins, Biological</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4488694</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ELISA</Keyword><Keyword MajorTopicYN="N">gluten</Keyword><Keyword MajorTopicYN="N">microwave</Keyword><Keyword MajorTopicYN="N">ricin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>4</Month><Day>24</Day></PubMedPubDate><PubMedPubDate 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