Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.
Identifieur interne : 000161 ( PubMed/Curation ); précédent : 000160; suivant : 000162Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.
Auteurs : Márcio Santos Soares [Brésil] ; Danielle Fernandes Da Silva [Brésil] ; Moacir Rossi Forim [Brésil] ; Maria Fátima Das Graças Fernandes Da Silva [Brésil] ; João Batista Fernandes [Brésil] ; Paulo Cezar Vieira [Brésil] ; Denise Brentan Silva [Brésil] ; Norberto Peporine Lopes [Brésil] ; Sérgio Alves De Carvalho [Brésil] ; Alessandra Alves De Souza [Brésil] ; Marcos Antônio Machado [Brésil]Source :
- Phytochemistry [ 1873-3700 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Hesperidin, Rutin.
- chemistry : Citrus, Plant Leaves, Plant Stems.
- drug effects : Xylella.
- genetics : Citrus.
- pathogenicity : Xylella.
- Chromatography, High Pressure Liquid, Nuclear Magnetic Resonance, Biomolecular.
Abstract
A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg mL(-1) respectively, with r(2)>0.999 for all curves. The limits of detection (LOD) for both flavonoids were 0.6 and 0.5 μg mL(-1), respectively, with quantification (LOQ) being 2.0 and 1.0 μg mL(-1), respectively. The quantification method was applied to Citrus sinensis grafted onto C. limonia with and without CVC (citrus variegated chlorosis) symptoms after Xylella fastidiosa infection. The total content of rutin was low and practically constant in all analyses in comparison with hesperidin, which showed a significant increase in its amount in symptomatic leaves. Scanning electron microscopy studies on leaves with CVC symptoms showed vessel occlusion by biofilm, and a crystallized material was noted. Considering the difficulty in isolating these crystals for analysis, tissue sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to confirm the presence of hesperidin at the site of infection. The images constructed from MS/MS data with a specific diagnostic fragment ion (m/z 483) also showed higher ion intensities for it in infected plants than in healthy ones, mainly in the vessel regions. These data suggest that hesperidin plays a role in the plant-pathogen interaction, probably as a phytoanticipin. This method was also applied to C. sinensis and C. limonia seedlings, and comparison with the graft results showed that the rootstock had an increased hesperidin content ∼3.6 fold greater in the graft stem than in the stem of C. sinensis seedlings. Increase in hesperidin content by rootstock can be related to induced internal defense mechanisms.
DOI: 10.1016/j.phytochem.2015.02.011
PubMed: 25749617
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Norberto Peporine" uniqKey="Lopes N" first="Norberto Peporine" last="Lopes">Norberto Peporine Lopes</name><affiliation wicri:level="1"><nlm:affiliation>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP</wicri:regionArea></affiliation></author><author><name sortKey="De Carvalho, Sergio Alves" sort="De Carvalho, Sergio Alves" uniqKey="De Carvalho S" first="Sérgio Alves" last="De Carvalho">Sérgio Alves De Carvalho</name><affiliation wicri:level="1"><nlm:affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo</wicri:regionArea></affiliation></author><author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name><affiliation wicri:level="1"><nlm:affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo</wicri:regionArea></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antônio" last="Machado">Marcos Antônio Machado</name><affiliation wicri:level="1"><nlm:affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25749617</idno><idno type="pmid">25749617</idno><idno type="doi">10.1016/j.phytochem.2015.02.011</idno><idno type="wicri:Area/PubMed/Corpus">000161</idno><idno type="wicri:Area/PubMed/Curation">000161</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.</title><author><name sortKey="Soares, Marcio Santos" sort="Soares, Marcio Santos" uniqKey="Soares M" first="Márcio Santos" last="Soares">Márcio Santos Soares</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP</wicri:regionArea></affiliation></author><author><name sortKey="Da Silva, Danielle Fernandes" sort="Da Silva, Danielle Fernandes" uniqKey="Da Silva D" first="Danielle Fernandes" last="Da Silva">Danielle Fernandes Da Silva</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP</wicri:regionArea></affiliation></author><author><name sortKey="Forim, Moacir Rossi" sort="Forim, Moacir Rossi" uniqKey="Forim M" first="Moacir Rossi" last="Forim">Moacir Rossi Forim</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP</wicri:regionArea></affiliation></author><author><name sortKey="Da Silva, Maria Fatima Das Gracas Fernandes" sort="Da Silva, Maria Fatima Das Gracas Fernandes" uniqKey="Da Silva M" first="Maria Fátima Das Graças Fernandes" last="Da Silva">Maria Fátima Das Graças Fernandes Da Silva</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil. Electronic address: dmfs@ufscar.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP</wicri:regionArea></affiliation></author><author><name sortKey="Fernandes, Joao Batista" sort="Fernandes, Joao Batista" uniqKey="Fernandes J" first="João Batista" last="Fernandes">João Batista Fernandes</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP</wicri:regionArea></affiliation></author><author><name sortKey="Vieira, Paulo Cezar" sort="Vieira, Paulo Cezar" uniqKey="Vieira P" first="Paulo Cezar" last="Vieira">Paulo Cezar Vieira</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP</wicri:regionArea></affiliation></author><author><name sortKey="Silva, Denise Brentan" sort="Silva, Denise Brentan" uniqKey="Silva D" first="Denise Brentan" last="Silva">Denise Brentan Silva</name><affiliation wicri:level="1"><nlm:affiliation>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP</wicri:regionArea></affiliation></author><author><name sortKey="Lopes, Norberto Peporine" sort="Lopes, Norberto Peporine" uniqKey="Lopes N" first="Norberto Peporine" last="Lopes">Norberto Peporine Lopes</name><affiliation wicri:level="1"><nlm:affiliation>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP</wicri:regionArea></affiliation></author><author><name sortKey="De Carvalho, Sergio Alves" sort="De Carvalho, Sergio Alves" uniqKey="De Carvalho S" first="Sérgio Alves" last="De Carvalho">Sérgio Alves De Carvalho</name><affiliation wicri:level="1"><nlm:affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo</wicri:regionArea></affiliation></author><author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name><affiliation wicri:level="1"><nlm:affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo</wicri:regionArea></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antônio" last="Machado">Marcos Antônio Machado</name><affiliation wicri:level="1"><nlm:affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo</wicri:regionArea></affiliation></author></analytic><series><title level="j">Phytochemistry</title><idno type="eISSN">1873-3700</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Citrus (chemistry)</term><term>Citrus (genetics)</term><term>Hesperidin (analysis)</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Plant Leaves (chemistry)</term><term>Plant Stems (chemistry)</term><term>Rutin (analysis)</term><term>Xylella (drug effects)</term><term>Xylella (pathogenicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hesperidin</term><term>Rutin</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term><term>Plant Leaves</term><term>Plant Stems</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Xylella</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Xylella</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Nuclear Magnetic Resonance, Biomolecular</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg mL(-1) respectively, with r(2)>0.999 for all curves. The limits of detection (LOD) for both flavonoids were 0.6 and 0.5 μg mL(-1), respectively, with quantification (LOQ) being 2.0 and 1.0 μg mL(-1), respectively. The quantification method was applied to Citrus sinensis grafted onto C. limonia with and without CVC (citrus variegated chlorosis) symptoms after Xylella fastidiosa infection. The total content of rutin was low and practically constant in all analyses in comparison with hesperidin, which showed a significant increase in its amount in symptomatic leaves. Scanning electron microscopy studies on leaves with CVC symptoms showed vessel occlusion by biofilm, and a crystallized material was noted. Considering the difficulty in isolating these crystals for analysis, tissue sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to confirm the presence of hesperidin at the site of infection. The images constructed from MS/MS data with a specific diagnostic fragment ion (m/z 483) also showed higher ion intensities for it in infected plants than in healthy ones, mainly in the vessel regions. These data suggest that hesperidin plays a role in the plant-pathogen interaction, probably as a phytoanticipin. This method was also applied to C. sinensis and C. limonia seedlings, and comparison with the graft results showed that the rootstock had an increased hesperidin content ∼3.6 fold greater in the graft stem than in the stem of C. sinensis seedlings. Increase in hesperidin content by rootstock can be related to induced internal defense mechanisms.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25749617</PMID><DateCreated><Year>2015</Year><Month>6</Month><Day>9</Day></DateCreated><DateCompleted><Year>2016</Year><Month>04</Month><Day>01</Day></DateCompleted><DateRevised><Year>2015</Year><Month>6</Month><Day>9</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3700</ISSN><JournalIssue CitedMedium="Internet"><Volume>115</Volume><PubDate><Year>2015</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.</ArticleTitle><Pagination><MedlinePgn>161-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.phytochem.2015.02.011</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0031-9422(15)00077-1</ELocationID><Abstract><AbstractText>A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg mL(-1) respectively, with r(2)>0.999 for all curves. The limits of detection (LOD) for both flavonoids were 0.6 and 0.5 μg mL(-1), respectively, with quantification (LOQ) being 2.0 and 1.0 μg mL(-1), respectively. The quantification method was applied to Citrus sinensis grafted onto C. limonia with and without CVC (citrus variegated chlorosis) symptoms after Xylella fastidiosa infection. The total content of rutin was low and practically constant in all analyses in comparison with hesperidin, which showed a significant increase in its amount in symptomatic leaves. Scanning electron microscopy studies on leaves with CVC symptoms showed vessel occlusion by biofilm, and a crystallized material was noted. Considering the difficulty in isolating these crystals for analysis, tissue sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to confirm the presence of hesperidin at the site of infection. The images constructed from MS/MS data with a specific diagnostic fragment ion (m/z 483) also showed higher ion intensities for it in infected plants than in healthy ones, mainly in the vessel regions. These data suggest that hesperidin plays a role in the plant-pathogen interaction, probably as a phytoanticipin. This method was also applied to C. sinensis and C. limonia seedlings, and comparison with the graft results showed that the rootstock had an increased hesperidin content ∼3.6 fold greater in the graft stem than in the stem of C. sinensis seedlings. Increase in hesperidin content by rootstock can be related to induced internal defense mechanisms.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Soares</LastName><ForeName>Márcio Santos</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>da Silva</LastName><ForeName>Danielle Fernandes</ForeName><Initials>DF</Initials><AffiliationInfo><Affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Forim</LastName><ForeName>Moacir Rossi</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>da Silva</LastName><ForeName>Maria Fátima das Graças Fernandes</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil. Electronic address: dmfs@ufscar.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernandes</LastName><ForeName>João Batista</ForeName><Initials>JB</Initials><AffiliationInfo><Affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vieira</LastName><ForeName>Paulo Cezar</ForeName><Initials>PC</Initials><AffiliationInfo><Affiliation>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Denise Brentan</ForeName><Initials>DB</Initials><AffiliationInfo><Affiliation>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lopes</LastName><ForeName>Norberto Peporine</ForeName><Initials>NP</Initials><AffiliationInfo><Affiliation>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Carvalho</LastName><ForeName>Sérgio Alves</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Souza</LastName><ForeName>Alessandra Alves</ForeName><Initials>AA</Initials><AffiliationInfo><Affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Machado</LastName><ForeName>Marcos Antônio</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>Mar</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>5G06TVY3R7</RegistryNumber><NameOfSubstance UI="D012431">Rutin</NameOfSubstance></Chemical><Chemical><RegistryNumber>E750O06Y6O</RegistryNumber><NameOfSubstance UI="D006569">Hesperidin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002957" MajorTopicYN="N">Citrus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006569" MajorTopicYN="N">Hesperidin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019906" MajorTopicYN="N">Nuclear Magnetic Resonance, Biomolecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012431" MajorTopicYN="N">Rutin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044167" MajorTopicYN="N">Xylella</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">C. sinensis</Keyword><Keyword MajorTopicYN="N">Citrus limonia</Keyword><Keyword MajorTopicYN="N">HPLC-UV</Keyword><Keyword MajorTopicYN="N">Hesperidin</Keyword><Keyword MajorTopicYN="N">MALDI MSI</Keyword><Keyword MajorTopicYN="N">Rutaceae</Keyword><Keyword MajorTopicYN="N">Xylella fastidiosa</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>6</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>1</Month><Day>2</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>2</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>4</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25749617</ArticleId><ArticleId IdType="pii">S0031-9422(15)00077-1</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2015.02.011</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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