Orange germin-like glycoprotein Cit s 1: an equivocal allergen.
Identifieur interne : 000C45 ( PubMed/Corpus ); précédent : 000C44; suivant : 000C46Orange germin-like glycoprotein Cit s 1: an equivocal allergen.
Auteurs : Oussama Ahrazem ; M Dolores Ibá Ez ; Gema L Pez-Torrej N ; Rosa Sánchez-Monge ; Joaquin Sastre ; Manuel Lombardero ; Domingo Barber ; Gabriel SalcedoSource :
- International archives of allergy and immunology [ 1018-2438 ] ; 2006.
English descriptors
- KwdEn :
- Adolescent, Adult, Allergens (chemistry), Allergens (immunology), Allergens (isolation & purification), Amino Acid Sequence, Child, Citrus sinensis (immunology), Female, Food Hypersensitivity (immunology), Glycoproteins (chemistry), Glycoproteins (immunology), Glycoproteins (isolation & purification), Humans, Immunoglobulin E (blood), Male, Molecular Sequence Data, Plant Proteins (chemistry), Plant Proteins (immunology), Plant Proteins (isolation & purification).
- MESH :
- chemical , blood : Immunoglobulin E.
- chemical , chemistry : Allergens, Glycoproteins, Plant Proteins.
- chemical , immunology : Allergens, Glycoproteins, Plant Proteins.
- chemical , isolation & purification : Allergens, Glycoproteins, Plant Proteins.
- immunology : Citrus sinensis, Food Hypersensitivity.
- Adolescent, Adult, Amino Acid Sequence, Child, Female, Humans, Male, Molecular Sequence Data.
Abstract
Orange allergens are virtually unknown, in spite of the large consumption of this fruit. Germin-like proteins, together with vicilins and legumins, form the cupin superfamily of plant proteins, which includes many seed allergens.
DOI: 10.1159/000090384
PubMed: 16357490
Links to Exploration step
pubmed:16357490Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Orange germin-like glycoprotein Cit s 1: an equivocal allergen.</title><author><name sortKey="Ahrazem, Oussama" sort="Ahrazem, Oussama" uniqKey="Ahrazem O" first="Oussama" last="Ahrazem">Oussama Ahrazem</name><affiliation><nlm:affiliation>Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, UPM, Madrid, España.</nlm:affiliation></affiliation></author><author><name sortKey="Iba Ez, M Dolores" sort="Iba Ez, M Dolores" uniqKey="Iba Ez M" first="M Dolores" last="Ibá Ez">M Dolores Ibá Ez</name></author><author><name sortKey="L Pez Torrej N, Gema" sort="L Pez Torrej N, Gema" uniqKey="L Pez Torrej N G" first="Gema" last="L Pez-Torrej N">Gema L Pez-Torrej N</name></author><author><name sortKey="Sanchez Monge, Rosa" sort="Sanchez Monge, Rosa" uniqKey="Sanchez Monge R" first="Rosa" last="Sánchez-Monge">Rosa Sánchez-Monge</name></author><author><name sortKey="Sastre, Joaquin" sort="Sastre, Joaquin" uniqKey="Sastre J" first="Joaquin" last="Sastre">Joaquin Sastre</name></author><author><name sortKey="Lombardero, Manuel" sort="Lombardero, Manuel" uniqKey="Lombardero M" first="Manuel" last="Lombardero">Manuel Lombardero</name></author><author><name sortKey="Barber, Domingo" sort="Barber, Domingo" uniqKey="Barber D" first="Domingo" last="Barber">Domingo Barber</name></author><author><name sortKey="Salcedo, Gabriel" sort="Salcedo, Gabriel" uniqKey="Salcedo G" first="Gabriel" last="Salcedo">Gabriel Salcedo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16357490</idno><idno type="pmid">16357490</idno><idno type="doi">10.1159/000090384</idno><idno type="wicri:Area/PubMed/Corpus">000C45</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Orange germin-like glycoprotein Cit s 1: an equivocal allergen.</title><author><name sortKey="Ahrazem, Oussama" sort="Ahrazem, Oussama" uniqKey="Ahrazem O" first="Oussama" last="Ahrazem">Oussama Ahrazem</name><affiliation><nlm:affiliation>Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, UPM, Madrid, España.</nlm:affiliation></affiliation></author><author><name sortKey="Iba Ez, M Dolores" sort="Iba Ez, M Dolores" uniqKey="Iba Ez M" first="M Dolores" last="Ibá Ez">M Dolores Ibá Ez</name></author><author><name sortKey="L Pez Torrej N, Gema" sort="L Pez Torrej N, Gema" uniqKey="L Pez Torrej N G" first="Gema" last="L Pez-Torrej N">Gema L Pez-Torrej N</name></author><author><name sortKey="Sanchez Monge, Rosa" sort="Sanchez Monge, Rosa" uniqKey="Sanchez Monge R" first="Rosa" last="Sánchez-Monge">Rosa Sánchez-Monge</name></author><author><name sortKey="Sastre, Joaquin" sort="Sastre, Joaquin" uniqKey="Sastre J" first="Joaquin" last="Sastre">Joaquin Sastre</name></author><author><name sortKey="Lombardero, Manuel" sort="Lombardero, Manuel" uniqKey="Lombardero M" first="Manuel" last="Lombardero">Manuel Lombardero</name></author><author><name sortKey="Barber, Domingo" sort="Barber, Domingo" uniqKey="Barber D" first="Domingo" last="Barber">Domingo Barber</name></author><author><name sortKey="Salcedo, Gabriel" sort="Salcedo, Gabriel" uniqKey="Salcedo G" first="Gabriel" last="Salcedo">Gabriel Salcedo</name></author></analytic><series><title level="j">International archives of allergy and immunology</title><idno type="ISSN">1018-2438</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Allergens (chemistry)</term><term>Allergens (immunology)</term><term>Allergens (isolation & purification)</term><term>Amino Acid Sequence</term><term>Child</term><term>Citrus sinensis (immunology)</term><term>Female</term><term>Food Hypersensitivity (immunology)</term><term>Glycoproteins (chemistry)</term><term>Glycoproteins (immunology)</term><term>Glycoproteins (isolation & purification)</term><term>Humans</term><term>Immunoglobulin E (blood)</term><term>Male</term><term>Molecular Sequence Data</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (immunology)</term><term>Plant Proteins (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin E</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Allergens</term><term>Glycoproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Allergens</term><term>Glycoproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Allergens</term><term>Glycoproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Citrus sinensis</term><term>Food Hypersensitivity</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Amino Acid Sequence</term><term>Child</term><term>Female</term><term>Humans</term><term>Male</term><term>Molecular Sequence Data</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Orange allergens are virtually unknown, in spite of the large consumption of this fruit. Germin-like proteins, together with vicilins and legumins, form the cupin superfamily of plant proteins, which includes many seed allergens.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16357490</PMID><DateCreated><Year>2006</Year><Month>2</Month><Day>1</Day></DateCreated><DateCompleted><Year>2006</Year><Month>03</Month><Day>15</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1018-2438</ISSN><JournalIssue CitedMedium="Print"><Volume>139</Volume><Issue>2</Issue><PubDate><Year>2006</Year></PubDate></JournalIssue><Title>International archives of allergy and immunology</Title><ISOAbbreviation>Int. Arch. Allergy Immunol.</ISOAbbreviation></Journal><ArticleTitle>Orange germin-like glycoprotein Cit s 1: an equivocal allergen.</ArticleTitle><Pagination><MedlinePgn>96-103</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Orange allergens are virtually unknown, in spite of the large consumption of this fruit. Germin-like proteins, together with vicilins and legumins, form the cupin superfamily of plant proteins, which includes many seed allergens.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Twenty-nine patients with allergy to oranges were studied. A major IgE-binding protein from orange extracts was isolated by means of a two-step cation-exchange chromatographic protocol. The allergen was characterized by N-terminal amino acid sequencing and MALDI analysis, and its reactivity explored by specific IgE determination in individual sera, ELISA inhibition assays and in vivo skin prick tests (SPT). Chemical deglycosylation of the purified allergen was achieved by trifluoromethylsulfonate acid treatment.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The 24-kDa purified allergen, designated Cit s 1, was identified as a germin-like glycoprotein, based on its N-terminal amino acid sequence, molecular size and recognition by rabbit anti-complex N-linked glycan antibodies. Specific IgE to Cit s 1 was detected in 62% of 29 individual sera from orange-allergic patients, whereas positive SPT responses to the purified allergen were obtained in only 10% of such patients. Deglycosylation of Cit s 1 resulted in a loss of its IgE-binding capacity. Moreover, the unrelated plant glycoprotein horseradish peroxidase inhibited over 70% the IgE-binding to Cit s 1.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Over 60% of patients with allergy to oranges show specific IgE to Cit s 1. However, the purified allergen exerts a low in vivo reactivity. Complex N-linked glycans seem to play a prominent role in the IgE-binding capacity of Cit s 1. This characteristic of Cit s 1, as well as of other orange glycoproteins, could lead to false positives if the diagnosis of allergy to oranges is mainly based on in vitro specific IgE determination.</AbstractText><CopyrightInformation>Copyright (c) 2006 S. Karger AG, Basel.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ahrazem</LastName><ForeName>Oussama</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, UPM, Madrid, España.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ibáñez</LastName><ForeName>M Dolores</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>López-Torrejón</LastName><ForeName>Gema</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Sánchez-Monge</LastName><ForeName>Rosa</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Sastre</LastName><ForeName>Joaquin</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Lombardero</LastName><ForeName>Manuel</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Barber</LastName><ForeName>Domingo</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Salcedo</LastName><ForeName>Gabriel</ForeName><Initials>G</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>Dec</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Int Arch Allergy Immunol</MedlineTA><NlmUniqueID>9211652</NlmUniqueID><ISSNLinking>1018-2438</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000485">Allergens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006023">Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>37341-29-0</RegistryNumber><NameOfSubstance UI="D007073">Immunoglobulin E</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000485" MajorTopicYN="N">Allergens</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005512" MajorTopicYN="N">Food Hypersensitivity</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006023" MajorTopicYN="N">Glycoproteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007073" MajorTopicYN="N">Immunoglobulin E</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" 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