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Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.

Identifieur interne : 000930 ( PubMed/Corpus ); précédent : 000929; suivant : 000931

Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.

Auteurs : Xu Lu ; Wei Zhou ; Feng Gao

Source :

Molecular biology reports [ 1573-4978 ] ; 2009.

RBID : pubmed:19005777

English descriptors

KwdEn :
- Acyltransferases (chemistry), Acyltransferases (genetics), Amino Acid Sequence, Chromosomes, Plant (enzymology), Citrus sinensis (enzymology), Citrus sinensis (genetics), Cloning, Molecular, Computational Biology, DNA, Plant (genetics), Genes, Plant, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phylogeny, Protein Structure, Secondary, Sequence Alignment, Sequence Homology, Amino Acid.
MESH :
- chemical , chemistry : Acyltransferases.
- chemical , genetics : Acyltransferases, DNA, Plant.
- enzymology : Chromosomes, Plant, Citrus sinensis.
- genetics : Citrus sinensis.
- Amino Acid Sequence, Cloning, Molecular, Computational Biology, Genes, Plant, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phylogeny, Protein Structure, Secondary, Sequence Alignment, Sequence Homology, Amino Acid.

Abstract

Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome.

DOI: 10.1007/s11033-008-9408-z
PubMed: 19005777

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pubmed:19005777

Le document en format XML

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<author><name sortKey="Lu, Xu" sort="Lu, Xu" uniqKey="Lu X" first="Xu" last="Lu">Xu Lu</name>
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<author><name sortKey="Zhou, Wei" sort="Zhou, Wei" uniqKey="Zhou W" first="Wei" last="Zhou">Wei Zhou</name>
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<author><name sortKey="Gao, Feng" sort="Gao, Feng" uniqKey="Gao F" first="Feng" last="Gao">Feng Gao</name>
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<author><name sortKey="Zhou, Wei" sort="Zhou, Wei" uniqKey="Zhou W" first="Wei" last="Zhou">Wei Zhou</name>
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<term>Chromosomes, Plant (enzymology)</term>
<term>Citrus sinensis (enzymology)</term>
<term>Citrus sinensis (genetics)</term>
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<term>In Situ Hybridization, Fluorescence</term>
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<term>Protein Structure, Secondary</term>
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<term>Sequence Homology, Amino Acid</term>
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<term>DNA, Plant</term>
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<term>Citrus sinensis</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
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<term>In Situ Hybridization, Fluorescence</term>
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<front><div type="abstract" xml:lang="en">Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome.</div>
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<Abstract><AbstractText>Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome.</AbstractText>
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Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.

Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki