A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].
Identifieur interne : 000688 ( PubMed/Corpus ); précédent : 000687; suivant : 000689A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].
Auteurs : Yunliu Zeng ; Zhiyong Pan ; Yuduan Ding ; Andan Zhu ; Hongbo Cao ; Qiang Xu ; Xiuxin DengSource :
- Journal of experimental botany [ 1460-2431 ] ; 2011.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Chloroplast Proteins, Enzymes, Plant Proteins.
- metabolism : Citrus sinensis, Plastids.
- methods : Proteomics.
- Blotting, Western, Gene Expression Regulation, Plant.
Abstract
Here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using Nycodenz density gradient centrifugation is reported. A GeLC-MS/MS shotgun approach was used to identify the proteins of pooled chromoplast samples. A total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. Based on the predicted functions of these identified plastid proteins, a large proportion (∼60%) of the chromoplast proteome of sweet orange is constituted by proteins involved in carbohydrate metabolism, amino acid/protein synthesis, and secondary metabolism. Of note, HDS (hydroxymethylbutenyl 4-diphosphate synthase), PAP (plastid-lipid-associated protein), and psHSPs (plastid small heat shock proteins) involved in the synthesis or storage of carotenoid and stress response are among the most abundant proteins identified. A comparison of chromoplast proteomes between sweet orange and tomato suggested a high level of conservation in a broad range of metabolic pathways. However, the citrus chromoplast was characterized by more extensive carotenoid synthesis, extensive amino acid synthesis without nitrogen assimilation, and evidence for lipid metabolism concerning jasmonic acid synthesis. In conclusion, this study provides an insight into the major metabolic pathways as well as some unique characteristics of the sweet orange chromoplasts at the whole proteome level.
DOI: 10.1093/jxb/err140
PubMed: 21841170
Links to Exploration step
pubmed:21841170Le document en format XML
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Osbeck].</title><author><name sortKey="Zeng, Yunliu" sort="Zeng, Yunliu" uniqKey="Zeng Y" first="Yunliu" last="Zeng">Yunliu Zeng</name><affiliation><nlm:affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name></author><author><name sortKey="Ding, Yuduan" sort="Ding, Yuduan" uniqKey="Ding Y" first="Yuduan" last="Ding">Yuduan Ding</name></author><author><name sortKey="Zhu, Andan" sort="Zhu, Andan" uniqKey="Zhu A" first="Andan" last="Zhu">Andan Zhu</name></author><author><name sortKey="Cao, Hongbo" sort="Cao, Hongbo" uniqKey="Cao H" first="Hongbo" last="Cao">Hongbo Cao</name></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21841170</idno><idno type="pmid">21841170</idno><idno type="doi">10.1093/jxb/err140</idno><idno type="wicri:Area/PubMed/Corpus">000688</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].</title><author><name sortKey="Zeng, Yunliu" sort="Zeng, Yunliu" uniqKey="Zeng Y" first="Yunliu" last="Zeng">Yunliu Zeng</name><affiliation><nlm:affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name></author><author><name sortKey="Ding, Yuduan" sort="Ding, Yuduan" uniqKey="Ding Y" first="Yuduan" last="Ding">Yuduan Ding</name></author><author><name sortKey="Zhu, Andan" sort="Zhu, Andan" uniqKey="Zhu A" first="Andan" last="Zhu">Andan Zhu</name></author><author><name sortKey="Cao, Hongbo" sort="Cao, Hongbo" uniqKey="Cao H" first="Hongbo" last="Cao">Hongbo Cao</name></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Western</term><term>Chloroplast Proteins (metabolism)</term><term>Citrus sinensis (metabolism)</term><term>Enzymes (metabolism)</term><term>Gene Expression Regulation, Plant</term><term>Plant Proteins (metabolism)</term><term>Plastids (metabolism)</term><term>Proteomics (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chloroplast Proteins</term><term>Enzymes</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term><term>Plastids</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Gene Expression Regulation, Plant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using Nycodenz density gradient centrifugation is reported. A GeLC-MS/MS shotgun approach was used to identify the proteins of pooled chromoplast samples. A total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. Based on the predicted functions of these identified plastid proteins, a large proportion (∼60%) of the chromoplast proteome of sweet orange is constituted by proteins involved in carbohydrate metabolism, amino acid/protein synthesis, and secondary metabolism. Of note, HDS (hydroxymethylbutenyl 4-diphosphate synthase), PAP (plastid-lipid-associated protein), and psHSPs (plastid small heat shock proteins) involved in the synthesis or storage of carotenoid and stress response are among the most abundant proteins identified. A comparison of chromoplast proteomes between sweet orange and tomato suggested a high level of conservation in a broad range of metabolic pathways. However, the citrus chromoplast was characterized by more extensive carotenoid synthesis, extensive amino acid synthesis without nitrogen assimilation, and evidence for lipid metabolism concerning jasmonic acid synthesis. In conclusion, this study provides an insight into the major metabolic pathways as well as some unique characteristics of the sweet orange chromoplasts at the whole proteome level.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21841170</PMID><DateCreated><Year>2011</Year><Month>11</Month><Day>24</Day></DateCreated><DateCompleted><Year>2012</Year><Month>03</Month><Day>28</Day></DateCompleted><DateRevised><Year>2015</Year><Month>2</Month><Day>4</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>62</Volume><Issue>15</Issue><PubDate><Year>2011</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J. Exp. Bot.</ISOAbbreviation></Journal><ArticleTitle>A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].</ArticleTitle><Pagination><MedlinePgn>5297-309</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/err140</ELocationID><Abstract><AbstractText>Here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using Nycodenz density gradient centrifugation is reported. A GeLC-MS/MS shotgun approach was used to identify the proteins of pooled chromoplast samples. A total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. Based on the predicted functions of these identified plastid proteins, a large proportion (∼60%) of the chromoplast proteome of sweet orange is constituted by proteins involved in carbohydrate metabolism, amino acid/protein synthesis, and secondary metabolism. Of note, HDS (hydroxymethylbutenyl 4-diphosphate synthase), PAP (plastid-lipid-associated protein), and psHSPs (plastid small heat shock proteins) involved in the synthesis or storage of carotenoid and stress response are among the most abundant proteins identified. A comparison of chromoplast proteomes between sweet orange and tomato suggested a high level of conservation in a broad range of metabolic pathways. However, the citrus chromoplast was characterized by more extensive carotenoid synthesis, extensive amino acid synthesis without nitrogen assimilation, and evidence for lipid metabolism concerning jasmonic acid synthesis. In conclusion, this study provides an insight into the major metabolic pathways as well as some unique characteristics of the sweet orange chromoplasts at the whole proteome level.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Yunliu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Zhiyong</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Yuduan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Andan</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Hongbo</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Xiuxin</ForeName><Initials>X</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>Aug</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D060365">Chloroplast Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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Version="1">16474971</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Cell Physiol. 2006 Mar;47(3):432-6</RefSource><PMID Version="1">16418230</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Exp Bot. 2006;57(7):1591-602</RefSource><PMID Version="1">16595579</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Annu Rev Plant Biol. 2006;57:599-621</RefSource><PMID Version="1">16669775</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Annu Rev Plant Biol. 2006;57:711-38</RefSource><PMID Version="1">16669779</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Physiol. 2006 Jun;141(2):391-6</RefSource><PMID Version="1">16760493</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Cell. 2007 Oct;19(10):3170-93</RefSource><PMID 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