Gene expression in Citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.
Identifieur interne : 000617 ( PubMed/Corpus ); précédent : 000616; suivant : 000618Gene expression in Citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.
Auteurs : Hui-Ling Liao ; Jacqueline K. BurnsSource :
- Journal of experimental botany [ 1460-2431 ] ; 2012.
English descriptors
- KwdEn :
- Carbohydrate Metabolism (genetics), Citrus sinensis (genetics), Citrus sinensis (microbiology), Expressed Sequence Tags, Fruit (anatomy & histology), Fruit (genetics), Fruit (microbiology), Gene Expression Regulation, Plant, Genes, Plant (genetics), Organ Specificity (genetics), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Leaves (microbiology), Plant Vascular Bundle (genetics), Plant Vascular Bundle (microbiology), RNA, Ribosomal, 16S (genetics), Real-Time Polymerase Chain Reaction, Rhizobiaceae (physiology), Trees (microbiology).
- MESH :
- chemical , genetics : RNA, Ribosomal, 16S.
- anatomy & histology : Fruit.
- genetics : Carbohydrate Metabolism, Citrus sinensis, Fruit, Genes, Plant, Organ Specificity, Plant Diseases, Plant Vascular Bundle.
- microbiology : Citrus sinensis, Fruit, Plant Diseases, Plant Leaves, Plant Vascular Bundle, Trees.
- physiology : Rhizobiaceae.
- Expressed Sequence Tags, Gene Expression Regulation, Plant, Real-Time Polymerase Chain Reaction.
Abstract
Distribution of viable Candidatus Liberibacter asiaticus (CaLas) in sweet orange fruit and leaves ('Hamlin' and 'Valencia') and transcriptomic changes associated with huanglongbing (HLB) infection in fruit tissues are reported. Viable CaLas was present in most fruit tissues tested in HLB trees, with the highest titre detected in vascular tissue near the calyx abscission zone. Transcriptomic changes associated with HLB infection were analysed in flavedo (FF), vascular tissue (VT), and juice vesicles (JV) from symptomatic (SY), asymptomatic (AS), and healthy (H) fruit. In SY 'Hamlin', HLB altered the expression of more genes in FF and VT than in JV, whereas in SY 'Valencia', the number of genes whose expression was changed by HLB was similar in these tissues. The expression of more genes was altered in SY 'Valencia' JV than in SY 'Hamlin' JV. More genes were also affected in AS 'Valencia' FF and VT than in AS 'Valencia' JV. Most genes whose expression was changed by HLB were classified as transporters or involved in carbohydrate metabolism. Physiological characteristics of HLB-infected and girdled fruit were compared to differentiate between HLB-specific and carbohydrate metabolism-related symptoms. SY and girdled fruit were smaller than H and ungirdled fruit, respectively, with poor juice quality. However, girdling did not cause misshapen fruit or differential peel coloration. Quantitative PCR analysis indicated that many selected genes changed their expression significantly in SY flavedo but not in girdled flavedo. Mechanisms regulating development of HLB symptoms may lie in the host disease response rather than being a direct consequence of carbohydrate starvation.
DOI: 10.1093/jxb/ers070
PubMed: 22407645
Links to Exploration step
pubmed:22407645Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Gene expression in Citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.</title><author><name sortKey="Liao, Hui Ling" sort="Liao, Hui Ling" uniqKey="Liao H" first="Hui-Ling" last="Liao">Hui-Ling Liao</name><affiliation><nlm:affiliation>University of Florida, IFAS, Horticultural Sciences Department, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850-2299, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K" last="Burns">Jacqueline K. Burns</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22407645</idno><idno type="pmid">22407645</idno><idno type="doi">10.1093/jxb/ers070</idno><idno type="wicri:Area/PubMed/Corpus">000617</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Gene expression in Citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.</title><author><name sortKey="Liao, Hui Ling" sort="Liao, Hui Ling" uniqKey="Liao H" first="Hui-Ling" last="Liao">Hui-Ling Liao</name><affiliation><nlm:affiliation>University of Florida, IFAS, Horticultural Sciences Department, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850-2299, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K" last="Burns">Jacqueline K. Burns</name></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbohydrate Metabolism (genetics)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (microbiology)</term><term>Expressed Sequence Tags</term><term>Fruit (anatomy & histology)</term><term>Fruit (genetics)</term><term>Fruit (microbiology)</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant (genetics)</term><term>Organ Specificity (genetics)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Plant Vascular Bundle (genetics)</term><term>Plant Vascular Bundle (microbiology)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Real-Time Polymerase Chain Reaction</term><term>Rhizobiaceae (physiology)</term><term>Trees (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Fruit</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Citrus sinensis</term><term>Fruit</term><term>Genes, Plant</term><term>Organ Specificity</term><term>Plant Diseases</term><term>Plant Vascular Bundle</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term><term>Plant Diseases</term><term>Plant Leaves</term><term>Plant Vascular Bundle</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Rhizobiaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Expressed Sequence Tags</term><term>Gene Expression Regulation, Plant</term><term>Real-Time Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Distribution of viable Candidatus Liberibacter asiaticus (CaLas) in sweet orange fruit and leaves ('Hamlin' and 'Valencia') and transcriptomic changes associated with huanglongbing (HLB) infection in fruit tissues are reported. Viable CaLas was present in most fruit tissues tested in HLB trees, with the highest titre detected in vascular tissue near the calyx abscission zone. Transcriptomic changes associated with HLB infection were analysed in flavedo (FF), vascular tissue (VT), and juice vesicles (JV) from symptomatic (SY), asymptomatic (AS), and healthy (H) fruit. In SY 'Hamlin', HLB altered the expression of more genes in FF and VT than in JV, whereas in SY 'Valencia', the number of genes whose expression was changed by HLB was similar in these tissues. The expression of more genes was altered in SY 'Valencia' JV than in SY 'Hamlin' JV. More genes were also affected in AS 'Valencia' FF and VT than in AS 'Valencia' JV. Most genes whose expression was changed by HLB were classified as transporters or involved in carbohydrate metabolism. Physiological characteristics of HLB-infected and girdled fruit were compared to differentiate between HLB-specific and carbohydrate metabolism-related symptoms. SY and girdled fruit were smaller than H and ungirdled fruit, respectively, with poor juice quality. However, girdling did not cause misshapen fruit or differential peel coloration. Quantitative PCR analysis indicated that many selected genes changed their expression significantly in SY flavedo but not in girdled flavedo. Mechanisms regulating development of HLB symptoms may lie in the host disease response rather than being a direct consequence of carbohydrate starvation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22407645</PMID><DateCreated><Year>2012</Year><Month>5</Month><Day>15</Day></DateCreated><DateCompleted><Year>2012</Year><Month>09</Month><Day>07</Day></DateCompleted><DateRevised><Year>2015</Year><Month>2</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>63</Volume><Issue>8</Issue><PubDate><Year>2012</Year><Month>May</Month></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J. Exp. Bot.</ISOAbbreviation></Journal><ArticleTitle>Gene expression in Citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.</ArticleTitle><Pagination><MedlinePgn>3307-19</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/ers070</ELocationID><Abstract><AbstractText>Distribution of viable Candidatus Liberibacter asiaticus (CaLas) in sweet orange fruit and leaves ('Hamlin' and 'Valencia') and transcriptomic changes associated with huanglongbing (HLB) infection in fruit tissues are reported. Viable CaLas was present in most fruit tissues tested in HLB trees, with the highest titre detected in vascular tissue near the calyx abscission zone. Transcriptomic changes associated with HLB infection were analysed in flavedo (FF), vascular tissue (VT), and juice vesicles (JV) from symptomatic (SY), asymptomatic (AS), and healthy (H) fruit. In SY 'Hamlin', HLB altered the expression of more genes in FF and VT than in JV, whereas in SY 'Valencia', the number of genes whose expression was changed by HLB was similar in these tissues. The expression of more genes was altered in SY 'Valencia' JV than in SY 'Hamlin' JV. More genes were also affected in AS 'Valencia' FF and VT than in AS 'Valencia' JV. Most genes whose expression was changed by HLB were classified as transporters or involved in carbohydrate metabolism. Physiological characteristics of HLB-infected and girdled fruit were compared to differentiate between HLB-specific and carbohydrate metabolism-related symptoms. SY and girdled fruit were smaller than H and ungirdled fruit, respectively, with poor juice quality. However, girdling did not cause misshapen fruit or differential peel coloration. Quantitative PCR analysis indicated that many selected genes changed their expression significantly in SY flavedo but not in girdled flavedo. Mechanisms regulating development of HLB symptoms may lie in the host disease response rather than being a direct consequence of carbohydrate starvation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liao</LastName><ForeName>Hui-Ling</ForeName><Initials>HL</Initials><AffiliationInfo><Affiliation>University of Florida, IFAS, Horticultural Sciences Department, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850-2299, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burns</LastName><ForeName>Jacqueline K</ForeName><Initials>JK</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>Mar</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Planta. 2000 Mar;210(4):636-43</RefSource><PMID Version="1">10787058</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Protoplasma. 2012 Jul;249(3):687-97</RefSource><PMID Version="1">21874517</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Cell. 2001 May;13(5):1221-30</RefSource><PMID Version="1">11340193</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Ann Bot. 2003 Jul;92(1):137-43</RefSource><PMID Version="1">12763756</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Exp Bot. 2003 Nov;54(392):2449-56</RefSource><PMID Version="1">14565949</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Planta. 2003 Nov;218(1):58-64</RefSource><PMID Version="1">12937983</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Mol Biol. 2003 Nov;53(5):701-14</RefSource><PMID Version="1">15010608</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Curr Microbiol. 1995 Mar;30(3):137-41</RefSource><PMID Version="1">7765847</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Physiol. 2004 Dec;136(4):4198-204</RefSource><PMID Version="1">15531709</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):1857-62</RefSource><PMID Version="1">16166678</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 2006 Apr 28;312(5773):523-4</RefSource><PMID Version="1">16645067</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Microbiol Methods. 2006 Jul;66(1):104-15</RefSource><PMID Version="1">16414133</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Exp Bot. 2006;57(14):3679-86</RefSource><PMID Version="1">16968877</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Physiol. 2008 Apr;146(4):1797-809</RefSource><PMID Version="1">18245454</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Phytopathology. 2008 Apr;98(4):387-96</RefSource><PMID Version="1">18944186</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Phytopathology. 2008 May;98(5):592-9</RefSource><PMID Version="1">18943228</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Phytopathology. 2009 Jan;99(1):50-7</RefSource><PMID Version="1">19055434</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Physiol. 2009 May;150(1):424-36</RefSource><PMID Version="1">19304931</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Physiol. 2009 Aug;150(4):1750-61</RefSource><PMID Version="1">19571312</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Plant Microbe Interact. 2009 Nov;22(11):1431-44</RefSource><PMID Version="1">19810812</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Phytopathology. 2009 Dec;99(12):1346-54</RefSource><PMID Version="1">19900000</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Agric Food Chem. 2010 Jan 27;58(2):1247-62</RefSource><PMID Version="1">20030384</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Agric Food Chem. 2010 May 26;58(10):6007-10</RefSource><PMID Version="1">20438136</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Food Sci. 2010 Mar;75(2):C199-207</RefSource><PMID Version="1">20492226</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Exp Bot. 2010 May;61(9):2469-78</RefSource><PMID Version="1">20388744</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Food Sci. 2010 May;75(4):S220-30</RefSource><PMID Version="1">20546425</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>New Phytol. 2010 Oct;188(1):161-74</RefSource><PMID Version="1">20704660</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Phytopathology. 2011 Oct;101(10):1242-50</RefSource><PMID Version="1">21714779</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Exp Bot. 2011 Oct;62(14):4851-61</RefSource><PMID Version="1">21725032</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Physiol. 2000 Oct;124(2):833-44</RefSource><PMID Version="1">11027731</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020224" MajorTopicYN="N">Expressed Sequence Tags</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009928" MajorTopicYN="N">Organ Specificity</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058526" MajorTopicYN="N">Plant Vascular Bundle</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012230" MajorTopicYN="N">Rhizobiaceae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><OtherID 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