Transcriptional profiling of canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) constitutively overexpressing a spermidine synthase gene.
Identifieur interne : 000506 ( PubMed/Corpus ); précédent : 000505; suivant : 000507Transcriptional profiling of canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) constitutively overexpressing a spermidine synthase gene.
Auteurs : Xing-Zheng Fu ; Ji-Hong LiuSource :
- BioMed research international [ 2314-6141 ] ; 2013.
English descriptors
- KwdEn :
- Cell Wall (metabolism), Citrus (genetics), Citrus (microbiology), DNA Primers, Disease Resistance (genetics), Gene Expression Profiling, Gene Expression Regulation, Plant, Genetic Engineering, Oligonucleotide Array Sequence Analysis, Plant Diseases (genetics), Plant Diseases (microbiology), Plant Leaves (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (microbiology), Spermidine Synthase (genetics), Spermidine Synthase (metabolism), Stress, Physiological, Transcription Factors (metabolism), Transcriptome, Xanthomonas.
- MESH :
- chemical , genetics : Spermidine Synthase.
- chemical , metabolism : Spermidine Synthase, Transcription Factors.
- chemical : DNA Primers.
- genetics : Citrus, Disease Resistance, Plant Diseases, Plants, Genetically Modified.
- metabolism : Cell Wall, Plant Leaves.
- microbiology : Citrus, Plant Diseases, Plants, Genetically Modified.
- Gene Expression Profiling, Gene Expression Regulation, Plant, Genetic Engineering, Oligonucleotide Array Sequence Analysis, Stress, Physiological, Transcriptome, Xanthomonas.
Abstract
Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating diseases affecting the citrus industry worldwide. In our previous study, the canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) plants were produced via constitutively overexpressing a spermidine synthase. To unravel the molecular mechanisms underlying Xcc resistance of the transgenic plants, in the present study global transcriptional profiling was compared between untransformed line (WT) and the transgenic line (TG9) by hybridizing with Affymetrix Citrus GeneChip. In total, 666 differentially expressed genes (DEGs) were identified, 448 upregulated, and 218 downregulated. The DEGs were classified into 33 categories after Gene ontology (GO) annotation, in which 68 genes are in response to stimulus and involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors. These genes and those related to starch and sucrose metabolism, glutathione metabolism, biosynthesis of phenylpropanoids, and plant hormones were hypothesized to play major roles in the canker resistance of TG9. Semiquantitative RT-PCR analysis showed that the transcript levels of several candidate genes in TG9 were significantly higher than in WT both before and after Xcc inoculation, indicating their potential association with canker disease.
DOI: 10.1155/2013/918136
PubMed: 23509803
Links to Exploration step
pubmed:23509803Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transcriptional profiling of canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) constitutively overexpressing a spermidine synthase gene.</title><author><name sortKey="Fu, Xing Zheng" sort="Fu, Xing Zheng" uniqKey="Fu X" first="Xing-Zheng" last="Fu">Xing-Zheng Fu</name><affiliation><nlm:affiliation>Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Ji Hong" sort="Liu, Ji Hong" uniqKey="Liu J" first="Ji-Hong" last="Liu">Ji-Hong Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23509803</idno><idno type="pmid">23509803</idno><idno type="doi">10.1155/2013/918136</idno><idno type="wicri:Area/PubMed/Corpus">000506</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transcriptional profiling of canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) constitutively overexpressing a spermidine synthase gene.</title><author><name sortKey="Fu, Xing Zheng" sort="Fu, Xing Zheng" uniqKey="Fu X" first="Xing-Zheng" last="Fu">Xing-Zheng Fu</name><affiliation><nlm:affiliation>Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Ji Hong" sort="Liu, Ji Hong" uniqKey="Liu J" first="Ji-Hong" last="Liu">Ji-Hong Liu</name></author></analytic><series><title level="j">BioMed research international</title><idno type="eISSN">2314-6141</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term><term>Citrus (genetics)</term><term>Citrus (microbiology)</term><term>DNA Primers</term><term>Disease Resistance (genetics)</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genetic Engineering</term><term>Oligonucleotide Array Sequence Analysis</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (microbiology)</term><term>Spermidine Synthase (genetics)</term><term>Spermidine Synthase (metabolism)</term><term>Stress, Physiological</term><term>Transcription Factors (metabolism)</term><term>Transcriptome</term><term>Xanthomonas</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Spermidine Synthase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Spermidine Synthase</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term><term>Disease Resistance</term><term>Plant Diseases</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus</term><term>Plant Diseases</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genetic Engineering</term><term>Oligonucleotide Array Sequence Analysis</term><term>Stress, Physiological</term><term>Transcriptome</term><term>Xanthomonas</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating diseases affecting the citrus industry worldwide. In our previous study, the canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) plants were produced via constitutively overexpressing a spermidine synthase. To unravel the molecular mechanisms underlying Xcc resistance of the transgenic plants, in the present study global transcriptional profiling was compared between untransformed line (WT) and the transgenic line (TG9) by hybridizing with Affymetrix Citrus GeneChip. In total, 666 differentially expressed genes (DEGs) were identified, 448 upregulated, and 218 downregulated. The DEGs were classified into 33 categories after Gene ontology (GO) annotation, in which 68 genes are in response to stimulus and involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors. These genes and those related to starch and sucrose metabolism, glutathione metabolism, biosynthesis of phenylpropanoids, and plant hormones were hypothesized to play major roles in the canker resistance of TG9. Semiquantitative RT-PCR analysis showed that the transcript levels of several candidate genes in TG9 were significantly higher than in WT both before and after Xcc inoculation, indicating their potential association with canker disease.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23509803</PMID><DateCreated><Year>2013</Year><Month>3</Month><Day>19</Day></DateCreated><DateCompleted><Year>2013</Year><Month>11</Month><Day>12</Day></DateCompleted><DateRevised><Year>2015</Year><Month>4</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2314-6141</ISSN><JournalIssue CitedMedium="Internet"><Volume>2013</Volume><PubDate><Year>2013</Year></PubDate></JournalIssue><Title>BioMed research international</Title><ISOAbbreviation>Biomed Res Int</ISOAbbreviation></Journal><ArticleTitle>Transcriptional profiling of canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) constitutively overexpressing a spermidine synthase gene.</ArticleTitle><Pagination><MedlinePgn>918136</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1155/2013/918136</ELocationID><Abstract><AbstractText>Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating diseases affecting the citrus industry worldwide. In our previous study, the canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) plants were produced via constitutively overexpressing a spermidine synthase. To unravel the molecular mechanisms underlying Xcc resistance of the transgenic plants, in the present study global transcriptional profiling was compared between untransformed line (WT) and the transgenic line (TG9) by hybridizing with Affymetrix Citrus GeneChip. In total, 666 differentially expressed genes (DEGs) were identified, 448 upregulated, and 218 downregulated. The DEGs were classified into 33 categories after Gene ontology (GO) annotation, in which 68 genes are in response to stimulus and involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors. These genes and those related to starch and sucrose metabolism, glutathione metabolism, biosynthesis of phenylpropanoids, and plant hormones were hypothesized to play major roles in the canker resistance of TG9. Semiquantitative RT-PCR analysis showed that the transcript levels of several candidate genes in TG9 were significantly higher than in WT both before and after Xcc inoculation, indicating their potential association with canker disease.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fu</LastName><ForeName>Xing-Zheng</ForeName><Initials>XZ</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Ji-Hong</ForeName><Initials>JH</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>Dec</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biomed Res Int</MedlineTA><NlmUniqueID>101600173</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.16</RegistryNumber><NameOfSubstance UI="D011436">Spermidine Synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Bioinformatics. 2005 Sep 15;21(18):3674-6</RefSource><PMID Version="1">16081474</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>BMC Plant Biol. 2011;11:55</RefSource><PMID 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MajorTopicYN="N">Citrus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011436" MajorTopicYN="N">Spermidine Synthase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014974" MajorTopicYN="Y">Xanthomonas</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC3591164</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>7</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>9</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>3</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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