MicroRNA Regulatory Mechanisms on Citrus sinensis leaves to Magnesium-Deficiency.
Identifieur interne : 000066 ( PubMed/Corpus ); précédent : 000065; suivant : 000067MicroRNA Regulatory Mechanisms on Citrus sinensis leaves to Magnesium-Deficiency.
Auteurs : Cui-Lan Ma ; Yi-Ping Qi ; Wei-Wei Liang ; Lin-Tong Yang ; Yi-Bin Lu ; Peng Guo ; Xin Ye ; Li-Song ChenSource :
- Frontiers in plant science ; 2016.
Abstract
Magnesium (Mg)-deficiency, which affects crop productivity and quality, widespreadly exists in many agricultural crops, including citrus. However, very limited data are available on Mg-deficiency-responsive microRNAs (miRNAs) in higher plants. Using Illumina sequencing, we isolated 75 (73 known and 2 novel) up- and 71 (64 known and 7 novel) down-regulated miRNAs from Mg-deficient Citrus sinensis leaves. In addition to the remarkable metabolic flexibility as indicated by the great alteration of miRNA expression, the adaptive responses of leaf miRNAs to Mg-deficiency might also involve the following several aspects: (a) up-regulating stress-related genes by down-regulating miR164, miR7812, miR5742, miR3946, and miR5158; (b) enhancing cell transport due to decreased expression of miR3946 and miR5158 and increased expression of miR395, miR1077, miR1160, and miR8019; (c) activating lipid metabolism-related genes by repressing miR158, miR5256, and miR3946; (d) inducing cell wall-related gene expansin 8A by repressing miR779; and (e) down-regulating the expression of genes involved in the maintenance of S, K and Cu by up-regulating miR395 and miR6426. To conclude, we isolated some new known miRNAs (i.e., miR7812, miR8019, miR6218, miR1533, miR6426, miR5256, miR5742, miR5561, miR5158, and miR5818) responsive to nutrient deficiencies and found some candidate miRNAs that might contribute to Mg-deficiency tolerance. Therefore, our results not only provide novel information about the responses of plant to Mg-deficiency, but also are useful for obtaining the key miRNAs for plant Mg-deficiency tolerance.
DOI: 10.3389/fpls.2016.00201
PubMed: 26973661
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College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Lin Tong" sort="Yang, Lin Tong" uniqKey="Yang L" first="Lin-Tong" last="Yang">Lin-Tong Yang</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lu, Yi Bin" sort="Lu, Yi Bin" uniqKey="Lu Y" first="Yi-Bin" last="Lu">Yi-Bin Lu</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Peng" sort="Guo, Peng" uniqKey="Guo P" first="Peng" last="Guo">Peng Guo</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ye, Xin" sort="Ye, Xin" uniqKey="Ye X" first="Xin" last="Ye">Xin Ye</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China; The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry UniversityFuzhou, China; Fujian Key Laboratory for Plant Molecular and Cell Biology, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26973661</idno><idno type="pmid">26973661</idno><idno type="doi">10.3389/fpls.2016.00201</idno><idno type="wicri:Area/PubMed/Corpus">000066</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">MicroRNA Regulatory Mechanisms on Citrus sinensis leaves to Magnesium-Deficiency.</title><author><name sortKey="Ma, Cui Lan" sort="Ma, Cui Lan" uniqKey="Ma C" first="Cui-Lan" last="Ma">Cui-Lan Ma</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Life Science, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Horticulture, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qi, Yi Ping" sort="Qi, Yi Ping" uniqKey="Qi Y" first="Yi-Ping" last="Qi">Yi-Ping Qi</name><affiliation><nlm:affiliation>Institute of Materia Medica, Fujian Academy of Medical Sciences Fuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liang, Wei Wei" sort="Liang, Wei Wei" uniqKey="Liang W" first="Wei-Wei" last="Liang">Wei-Wei Liang</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Lin Tong" sort="Yang, Lin Tong" uniqKey="Yang L" first="Lin-Tong" last="Yang">Lin-Tong Yang</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lu, Yi Bin" sort="Lu, Yi Bin" uniqKey="Lu Y" first="Yi-Bin" last="Lu">Yi-Bin Lu</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Peng" sort="Guo, Peng" uniqKey="Guo P" first="Peng" last="Guo">Peng Guo</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ye, Xin" sort="Ye, Xin" uniqKey="Ye X" first="Xin" last="Ye">Xin Ye</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name><affiliation><nlm:affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China; The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry UniversityFuzhou, China; Fujian Key Laboratory for Plant Molecular and Cell Biology, Fujian Agriculture and Forestry UniversityFuzhou, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Magnesium (Mg)-deficiency, which affects crop productivity and quality, widespreadly exists in many agricultural crops, including citrus. However, very limited data are available on Mg-deficiency-responsive microRNAs (miRNAs) in higher plants. Using Illumina sequencing, we isolated 75 (73 known and 2 novel) up- and 71 (64 known and 7 novel) down-regulated miRNAs from Mg-deficient Citrus sinensis leaves. In addition to the remarkable metabolic flexibility as indicated by the great alteration of miRNA expression, the adaptive responses of leaf miRNAs to Mg-deficiency might also involve the following several aspects: (a) up-regulating stress-related genes by down-regulating miR164, miR7812, miR5742, miR3946, and miR5158; (b) enhancing cell transport due to decreased expression of miR3946 and miR5158 and increased expression of miR395, miR1077, miR1160, and miR8019; (c) activating lipid metabolism-related genes by repressing miR158, miR5256, and miR3946; (d) inducing cell wall-related gene expansin 8A by repressing miR779; and (e) down-regulating the expression of genes involved in the maintenance of S, K and Cu by up-regulating miR395 and miR6426. To conclude, we isolated some new known miRNAs (i.e., miR7812, miR8019, miR6218, miR1533, miR6426, miR5256, miR5742, miR5561, miR5158, and miR5818) responsive to nutrient deficiencies and found some candidate miRNAs that might contribute to Mg-deficiency tolerance. Therefore, our results not only provide novel information about the responses of plant to Mg-deficiency, but also are useful for obtaining the key miRNAs for plant Mg-deficiency tolerance.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">26973661</PMID><DateCreated><Year>2016</Year><Month>3</Month><Day>14</Day></DateCreated><DateCompleted><Year>2016</Year><Month>03</Month><Day>14</Day></DateCompleted><DateRevised><Year>2016</Year><Month>3</Month><Day>16</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><JournalIssue CitedMedium="Print"><Volume>7</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>MicroRNA Regulatory Mechanisms on Citrus sinensis leaves to Magnesium-Deficiency.</ArticleTitle><Pagination><MedlinePgn>201</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2016.00201</ELocationID><Abstract><AbstractText>Magnesium (Mg)-deficiency, which affects crop productivity and quality, widespreadly exists in many agricultural crops, including citrus. However, very limited data are available on Mg-deficiency-responsive microRNAs (miRNAs) in higher plants. Using Illumina sequencing, we isolated 75 (73 known and 2 novel) up- and 71 (64 known and 7 novel) down-regulated miRNAs from Mg-deficient Citrus sinensis leaves. In addition to the remarkable metabolic flexibility as indicated by the great alteration of miRNA expression, the adaptive responses of leaf miRNAs to Mg-deficiency might also involve the following several aspects: (a) up-regulating stress-related genes by down-regulating miR164, miR7812, miR5742, miR3946, and miR5158; (b) enhancing cell transport due to decreased expression of miR3946 and miR5158 and increased expression of miR395, miR1077, miR1160, and miR8019; (c) activating lipid metabolism-related genes by repressing miR158, miR5256, and miR3946; (d) inducing cell wall-related gene expansin 8A by repressing miR779; and (e) down-regulating the expression of genes involved in the maintenance of S, K and Cu by up-regulating miR395 and miR6426. To conclude, we isolated some new known miRNAs (i.e., miR7812, miR8019, miR6218, miR1533, miR6426, miR5256, miR5742, miR5561, miR5158, and miR5818) responsive to nutrient deficiencies and found some candidate miRNAs that might contribute to Mg-deficiency tolerance. Therefore, our results not only provide novel information about the responses of plant to Mg-deficiency, but also are useful for obtaining the key miRNAs for plant Mg-deficiency tolerance.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Cui-Lan</ForeName><Initials>CL</Initials><AffiliationInfo><Affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Life Science, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Horticulture, Fujian Agriculture and Forestry UniversityFuzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Yi-Ping</ForeName><Initials>YP</Initials><AffiliationInfo><Affiliation>Institute of Materia Medica, Fujian Academy of Medical Sciences Fuzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Wei-Wei</ForeName><Initials>WW</Initials><AffiliationInfo><Affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Lin-Tong</ForeName><Initials>LT</Initials><AffiliationInfo><Affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Yi-Bin</ForeName><Initials>YB</Initials><AffiliationInfo><Affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Xin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Li-Song</ForeName><Initials>LS</Initials><AffiliationInfo><Affiliation>Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China; The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry UniversityFuzhou, China; Fujian Key Laboratory for Plant Molecular and Cell Biology, Fujian Agriculture and Forestry UniversityFuzhou, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>Mar</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><OtherID Source="NLM">PMC4778066</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Citrus sinensis</Keyword><Keyword MajorTopicYN="N">Illumina sequencing</Keyword><Keyword MajorTopicYN="N">Mg-deficiency</Keyword><Keyword MajorTopicYN="N">leaves</Keyword><Keyword MajorTopicYN="N">microRNA</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>9</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>2</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>15</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26973661</ArticleId><ArticleId IdType="doi">10.3389/fpls.2016.00201</ArticleId><ArticleId IdType="pmc">PMC4778066</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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