Laser-induced Fluorescence Spectroscopy (LIFS) for Discrimination of Genetically Close Sweet Orange Accessions (Citrus sinensis L. Osbeck).
Identifieur interne : 000029 ( PubMed/Corpus ); précédent : 000028; suivant : 000030Laser-induced Fluorescence Spectroscopy (LIFS) for Discrimination of Genetically Close Sweet Orange Accessions (Citrus sinensis L. Osbeck).
Auteurs : Thiago Massaiti Kuboyama Kubota ; Aida Bebeachibuli Magalhães ; Marina Nery Da Silva ; Paulino Ribeiro Villas Boas ; Valdenice M. Novelli ; Marinês Bastianel ; Cíntia H D. Sagawa ; Mariângela Cristofani-Yaly ; Débora Marcondes Bastos Pereira MiloriSource :
- Applied spectroscopy [ 1943-3530 ] ; 2016.
Abstract
Although there is substantial diversity among cultivated sweet oranges genotypes with respect to morphological, physiological, and agronomic traits, very little variation at DNA level has been observed. It is possible that this low DNA molecular variability is due to a narrow genetic basis commonly observed in this citrus group. The most different morphological characters observed were originated through mutations, which are maintained by vegetative propagation. Despite all molecular tools available for discrimination between these different accessions, in general, low polymorphism has been observed in all groups of sweet oranges and they may not be identified by molecular markers. In this context, this paper describes the results obtained by using laser-induced fluorescent spectroscopy (LIFS) as a tool to discriminate sweet orange accessions (Citrus sinensis L. Osbeck) including common, low acidity, pigmented, and navel orange groups, with very little variation at DNA level. The findings showed that LIFS combined with statistical methods is capable to discriminate different accessions. The basic idea is that citrus leaves have multiple fluorophores and concentration depends on their genetics and metabolism. Thus, we consider that the optical properties of citrus leaves may be different, depending on variety. The results have shown that the developed method, for the best classification rate, reaches an average sensitivity and specificity of 95% and 97.5%, respectively. An interesting application of this study is the development of an economically viable tool for early identification in seedling certification, in citrus breeding programs, in cultivar protection, or in germplasm core collection.
DOI: 10.1177/0003702816658668
PubMed: 27402688
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Osbeck).</title><author><name sortKey="Massaiti Kuboyama Kubota, Thiago" sort="Massaiti Kuboyama Kubota, Thiago" uniqKey="Massaiti Kuboyama Kubota T" first="Thiago" last="Massaiti Kuboyama Kubota">Thiago Massaiti Kuboyama Kubota</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil Sao Carlos Institute of Physics, University of Sao Paulo, São Carlos, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Bebeachibuli Magalhaes, Aida" sort="Bebeachibuli Magalhaes, Aida" uniqKey="Bebeachibuli Magalhaes A" first="Aida" last="Bebeachibuli Magalhães">Aida Bebeachibuli Magalhães</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil habibe.aida@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Nery Da Silva, Marina" sort="Nery Da Silva, Marina" uniqKey="Nery Da Silva M" first="Marina" last="Nery Da Silva">Marina Nery Da Silva</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Ribeiro Villas Boas, Paulino" sort="Ribeiro Villas Boas, Paulino" uniqKey="Ribeiro Villas Boas P" first="Paulino" last="Ribeiro Villas Boas">Paulino Ribeiro Villas Boas</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Novelli, Valdenice M" sort="Novelli, Valdenice M" uniqKey="Novelli V" first="Valdenice M" last="Novelli">Valdenice M. Novelli</name><affiliation><nlm:affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Bastianel, Marines" sort="Bastianel, Marines" uniqKey="Bastianel M" first="Marinês" last="Bastianel">Marinês Bastianel</name><affiliation><nlm:affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Sagawa, Cintia H D" sort="Sagawa, Cintia H D" uniqKey="Sagawa C" first="Cíntia H D" last="Sagawa">Cíntia H D. 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Osbeck).</title><author><name sortKey="Massaiti Kuboyama Kubota, Thiago" sort="Massaiti Kuboyama Kubota, Thiago" uniqKey="Massaiti Kuboyama Kubota T" first="Thiago" last="Massaiti Kuboyama Kubota">Thiago Massaiti Kuboyama Kubota</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil Sao Carlos Institute of Physics, University of Sao Paulo, São Carlos, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Bebeachibuli Magalhaes, Aida" sort="Bebeachibuli Magalhaes, Aida" uniqKey="Bebeachibuli Magalhaes A" first="Aida" last="Bebeachibuli Magalhães">Aida Bebeachibuli Magalhães</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil habibe.aida@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Nery Da Silva, Marina" sort="Nery Da Silva, Marina" uniqKey="Nery Da Silva M" first="Marina" last="Nery Da Silva">Marina Nery Da Silva</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Ribeiro Villas Boas, Paulino" sort="Ribeiro Villas Boas, Paulino" uniqKey="Ribeiro Villas Boas P" first="Paulino" last="Ribeiro Villas Boas">Paulino Ribeiro Villas Boas</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Novelli, Valdenice M" sort="Novelli, Valdenice M" uniqKey="Novelli V" first="Valdenice M" last="Novelli">Valdenice M. Novelli</name><affiliation><nlm:affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Bastianel, Marines" sort="Bastianel, Marines" uniqKey="Bastianel M" first="Marinês" last="Bastianel">Marinês Bastianel</name><affiliation><nlm:affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Sagawa, Cintia H D" sort="Sagawa, Cintia H D" uniqKey="Sagawa C" first="Cíntia H D" last="Sagawa">Cíntia H D. Sagawa</name><affiliation><nlm:affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Cristofani Yaly, Mariangela" sort="Cristofani Yaly, Mariangela" uniqKey="Cristofani Yaly M" first="Mariângela" last="Cristofani-Yaly">Mariângela Cristofani-Yaly</name><affiliation><nlm:affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Marcondes Bastos Pereira Milori, Debora" sort="Marcondes Bastos Pereira Milori, Debora" uniqKey="Marcondes Bastos Pereira Milori D" first="Débora" last="Marcondes Bastos Pereira Milori">Débora Marcondes Bastos Pereira Milori</name><affiliation><nlm:affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Applied spectroscopy</title><idno type="eISSN">1943-3530</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although there is substantial diversity among cultivated sweet oranges genotypes with respect to morphological, physiological, and agronomic traits, very little variation at DNA level has been observed. It is possible that this low DNA molecular variability is due to a narrow genetic basis commonly observed in this citrus group. The most different morphological characters observed were originated through mutations, which are maintained by vegetative propagation. Despite all molecular tools available for discrimination between these different accessions, in general, low polymorphism has been observed in all groups of sweet oranges and they may not be identified by molecular markers. In this context, this paper describes the results obtained by using laser-induced fluorescent spectroscopy (LIFS) as a tool to discriminate sweet orange accessions (Citrus sinensis L. Osbeck) including common, low acidity, pigmented, and navel orange groups, with very little variation at DNA level. The findings showed that LIFS combined with statistical methods is capable to discriminate different accessions. The basic idea is that citrus leaves have multiple fluorophores and concentration depends on their genetics and metabolism. Thus, we consider that the optical properties of citrus leaves may be different, depending on variety. The results have shown that the developed method, for the best classification rate, reaches an average sensitivity and specificity of 95% and 97.5%, respectively. An interesting application of this study is the development of an economically viable tool for early identification in seedling certification, in citrus breeding programs, in cultivar protection, or in germplasm core collection.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">27402688</PMID><DateCreated><Year>2016</Year><Month>7</Month><Day>12</Day></DateCreated><DateRevised><Year>2016</Year><Month>7</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1943-3530</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2016</Year><Month>Jul</Month><Day>8</Day></PubDate></JournalIssue><Title>Applied spectroscopy</Title><ISOAbbreviation>Appl Spectrosc</ISOAbbreviation></Journal><ArticleTitle>Laser-induced Fluorescence Spectroscopy (LIFS) for Discrimination of Genetically Close Sweet Orange Accessions (Citrus sinensis L. Osbeck).</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">0003702816658668</ELocationID><Abstract><AbstractText NlmCategory="UNASSIGNED">Although there is substantial diversity among cultivated sweet oranges genotypes with respect to morphological, physiological, and agronomic traits, very little variation at DNA level has been observed. It is possible that this low DNA molecular variability is due to a narrow genetic basis commonly observed in this citrus group. The most different morphological characters observed were originated through mutations, which are maintained by vegetative propagation. Despite all molecular tools available for discrimination between these different accessions, in general, low polymorphism has been observed in all groups of sweet oranges and they may not be identified by molecular markers. In this context, this paper describes the results obtained by using laser-induced fluorescent spectroscopy (LIFS) as a tool to discriminate sweet orange accessions (Citrus sinensis L. Osbeck) including common, low acidity, pigmented, and navel orange groups, with very little variation at DNA level. The findings showed that LIFS combined with statistical methods is capable to discriminate different accessions. The basic idea is that citrus leaves have multiple fluorophores and concentration depends on their genetics and metabolism. Thus, we consider that the optical properties of citrus leaves may be different, depending on variety. The results have shown that the developed method, for the best classification rate, reaches an average sensitivity and specificity of 95% and 97.5%, respectively. An interesting application of this study is the development of an economically viable tool for early identification in seedling certification, in citrus breeding programs, in cultivar protection, or in germplasm core collection.</AbstractText><CopyrightInformation>© The Author(s) 2016.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Massaiti Kuboyama Kubota</LastName><ForeName>Thiago</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil Sao Carlos Institute of Physics, University of Sao Paulo, São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bebeachibuli Magalhães</LastName><ForeName>Aida</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil habibe.aida@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nery da Silva</LastName><ForeName>Marina</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ribeiro Villas Boas</LastName><ForeName>Paulino</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Novelli</LastName><ForeName>Valdenice M</ForeName><Initials>VM</Initials><AffiliationInfo><Affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bastianel</LastName><ForeName>Marinês</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sagawa</LastName><ForeName>Cíntia H D</ForeName><Initials>CH</Initials><AffiliationInfo><Affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cristofani-Yaly</LastName><ForeName>Mariângela</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centro de Citricultura Sylvio Moreira of Instituto Agronômico (CCSM-IAC), Cordeirópolis, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marcondes Bastos Pereira Milori</LastName><ForeName>Débora</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Embrapa Instrumentation, São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="">JOURNAL ARTICLE</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>Jul</Month><Day>8</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Spectrosc</MedlineTA><NlmUniqueID>0372406</NlmUniqueID><ISSNLinking>0003-7028</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">LIFS</Keyword><Keyword MajorTopicYN="N">Laser-induced fluorescence spectroscopy</Keyword><Keyword MajorTopicYN="N">citrus leaf fluorophores</Keyword><Keyword MajorTopicYN="N">differentiation of very close orange varieties</Keyword><Keyword MajorTopicYN="N">instrumentation</Keyword><Keyword MajorTopicYN="N">sweet orange accession</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>6</Month><Day>2</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>7</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>7</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>7</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27402688</ArticleId><ArticleId IdType="pii">0003702816658668</ArticleId><ArticleId IdType="doi">10.1177/0003702816658668</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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