Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis).
Identifieur interne : 000024 ( PubMed/Corpus ); précédent : 000023; suivant : 000025Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis).
Auteurs : Xiaopeng Lu ; Xiongjun Cao ; Feifei Li ; Jing Li ; Jiang Xiong ; Guiyou Long ; Shangyin Cao ; Shenxi XieSource :
- Physiologia plantarum [ 1399-3054 ] ; 2016.
Abstract
Citrate, the predominant organic acid in citrus, determines the taste of these fruits. However, little is known about the synergic molecular processes regulating citrate accumulation. Using 'Dahongtiancheng' (Citrus sinensis) and 'Bingtangcheng' (C. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'Dahongtiancheng' and 'Bingtangcheng' exhibit significantly different patterns in citrate accumulation throughout fruit development, with the largest differences observed at 50-70 days after full bloom (DAFB). Comparative transcriptome profiling was performed for the endocarps of both cultivars at 50 and 70 DAFB. Over 34.5 million clean reads per library were successfully mapped to the reference database and 670-2630 differentially expressed genes (DEGs) were found in four libraries. Among the genes, five transcription factors were ascertained to be the candidates regulating citrate accumulation. Functional assignments of the DEGs indicated that photosynthesis, the citrate cycle and amino acid metabolism were significantly altered in 'Dahongtiancheng'. Physiological and molecular analyses suggested that high photosynthetic efficiency and partial impairment of citrate catabolism were crucial for the high-citrate trait, and amino acid biosynthesis was one of the important directions for citrate flux. The results reveal a global insight into the gene expression changes in a high-citrate compared with a low-citrate sweet orange. High accumulating efficiency and impaired degradation of citrate may be associated with the high-citrate trait of 'Dahongtiancheng'. Findings in this study increase understanding of the molecular processes regulating citrate accumulation in sweet orange.
DOI: 10.1111/ppl.12484
PubMed: 27507765
Links to Exploration step
pubmed:27507765Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis).</title><author><name sortKey="Lu, Xiaopeng" sort="Lu, Xiaopeng" uniqKey="Lu X" first="Xiaopeng" last="Lu">Xiaopeng Lu</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Xiongjun" sort="Cao, Xiongjun" uniqKey="Cao X" first="Xiongjun" last="Cao">Xiongjun Cao</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Feifei" sort="Li, Feifei" uniqKey="Li F" first="Feifei" last="Li">Feifei Li</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xiong, Jiang" sort="Xiong, Jiang" uniqKey="Xiong J" first="Jiang" last="Xiong">Jiang Xiong</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Long, Guiyou" sort="Long, Guiyou" uniqKey="Long G" first="Guiyou" last="Long">Guiyou Long</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Shangyin" sort="Cao, Shangyin" uniqKey="Cao S" first="Shangyin" last="Cao">Shangyin Cao</name><affiliation><nlm:affiliation>Zhengzhou Fruit Research Institute, Chinese academy of Agricultural Sciences, Zhengzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xie, Shenxi" sort="Xie, Shenxi" uniqKey="Xie S" first="Shenxi" last="Xie">Shenxi Xie</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China. shenxixie@163.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27507765</idno><idno type="pmid">27507765</idno><idno type="doi">10.1111/ppl.12484</idno><idno type="wicri:Area/PubMed/Corpus">000024</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis).</title><author><name sortKey="Lu, Xiaopeng" sort="Lu, Xiaopeng" uniqKey="Lu X" first="Xiaopeng" last="Lu">Xiaopeng Lu</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Xiongjun" sort="Cao, Xiongjun" uniqKey="Cao X" first="Xiongjun" last="Cao">Xiongjun Cao</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Feifei" sort="Li, Feifei" uniqKey="Li F" first="Feifei" last="Li">Feifei Li</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xiong, Jiang" sort="Xiong, Jiang" uniqKey="Xiong J" first="Jiang" last="Xiong">Jiang Xiong</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Long, Guiyou" sort="Long, Guiyou" uniqKey="Long G" first="Guiyou" last="Long">Guiyou Long</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Shangyin" sort="Cao, Shangyin" uniqKey="Cao S" first="Shangyin" last="Cao">Shangyin Cao</name><affiliation><nlm:affiliation>Zhengzhou Fruit Research Institute, Chinese academy of Agricultural Sciences, Zhengzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xie, Shenxi" sort="Xie, Shenxi" uniqKey="Xie S" first="Shenxi" last="Xie">Shenxi Xie</name><affiliation><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China. shenxixie@163.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrate, the predominant organic acid in citrus, determines the taste of these fruits. However, little is known about the synergic molecular processes regulating citrate accumulation. Using 'Dahongtiancheng' (Citrus sinensis) and 'Bingtangcheng' (C. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'Dahongtiancheng' and 'Bingtangcheng' exhibit significantly different patterns in citrate accumulation throughout fruit development, with the largest differences observed at 50-70 days after full bloom (DAFB). Comparative transcriptome profiling was performed for the endocarps of both cultivars at 50 and 70 DAFB. Over 34.5 million clean reads per library were successfully mapped to the reference database and 670-2630 differentially expressed genes (DEGs) were found in four libraries. Among the genes, five transcription factors were ascertained to be the candidates regulating citrate accumulation. Functional assignments of the DEGs indicated that photosynthesis, the citrate cycle and amino acid metabolism were significantly altered in 'Dahongtiancheng'. Physiological and molecular analyses suggested that high photosynthetic efficiency and partial impairment of citrate catabolism were crucial for the high-citrate trait, and amino acid biosynthesis was one of the important directions for citrate flux. The results reveal a global insight into the gene expression changes in a high-citrate compared with a low-citrate sweet orange. High accumulating efficiency and impaired degradation of citrate may be associated with the high-citrate trait of 'Dahongtiancheng'. Findings in this study increase understanding of the molecular processes regulating citrate accumulation in sweet orange.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">27507765</PMID><DateCreated><Year>2016</Year><Month>9</Month><Day>14</Day></DateCreated><DateRevised><Year>2016</Year><Month>9</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2016</Year><Month>Aug</Month><Day>10</Day></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis).</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/ppl.12484</ELocationID><Abstract><AbstractText NlmCategory="UNASSIGNED">Citrate, the predominant organic acid in citrus, determines the taste of these fruits. However, little is known about the synergic molecular processes regulating citrate accumulation. Using 'Dahongtiancheng' (Citrus sinensis) and 'Bingtangcheng' (C. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'Dahongtiancheng' and 'Bingtangcheng' exhibit significantly different patterns in citrate accumulation throughout fruit development, with the largest differences observed at 50-70 days after full bloom (DAFB). Comparative transcriptome profiling was performed for the endocarps of both cultivars at 50 and 70 DAFB. Over 34.5 million clean reads per library were successfully mapped to the reference database and 670-2630 differentially expressed genes (DEGs) were found in four libraries. Among the genes, five transcription factors were ascertained to be the candidates regulating citrate accumulation. Functional assignments of the DEGs indicated that photosynthesis, the citrate cycle and amino acid metabolism were significantly altered in 'Dahongtiancheng'. Physiological and molecular analyses suggested that high photosynthetic efficiency and partial impairment of citrate catabolism were crucial for the high-citrate trait, and amino acid biosynthesis was one of the important directions for citrate flux. The results reveal a global insight into the gene expression changes in a high-citrate compared with a low-citrate sweet orange. High accumulating efficiency and impaired degradation of citrate may be associated with the high-citrate trait of 'Dahongtiancheng'. Findings in this study increase understanding of the molecular processes regulating citrate accumulation in sweet orange.</AbstractText><CopyrightInformation>© 2016 Scandinavian Plant Physiology Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Xiaopeng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Centre for Citrus Improvement, Changsha, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Xiongjun</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Centre for Citrus Improvement, Changsha, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Feifei</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Horticulture, Hunan Academy of Agricultural Science, Changsha, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Centre for Citrus Improvement, Changsha, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiong</LastName><ForeName>Jiang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Centre for Citrus Improvement, Changsha, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Long</LastName><ForeName>Guiyou</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Centre for Citrus Improvement, Changsha, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Shangyin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Zhengzhou Fruit Research Institute, Chinese academy of Agricultural Sciences, Zhengzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Shenxi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China. shenxixie@163.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Centre for Citrus Improvement, Changsha, China. shenxixie@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="">JOURNAL ARTICLE</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>Aug</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>4</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>5</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>6</Month><Day>2</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27507765</ArticleId><ArticleId IdType="doi">10.1111/ppl.12484</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000024 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000024 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:27507765
|texte= Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:27507765" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |