Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).
Identifieur interne : 000E35 ( PubMed/Checkpoint ); précédent : 000E34; suivant : 000E36Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).
Auteurs : C. Betz [États-Unis] ; T G Mccollum ; R T MayerSource :
- Plant molecular biology [ 0167-4412 ] ; 2001.
English descriptors
- KwdEn :
- Citrus (enzymology), Cytochrome P-450 Enzyme System (genetics), DNA, Complementary, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Mixed Function Oxygenases (genetics), Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Trans-Cinnamate 4-Monooxygenase.
- MESH :
- chemical , genetics : Cytochrome P-450 Enzyme System, Mixed Function Oxygenases.
- enzymology : Citrus.
- chemical : DNA, Complementary, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Trans-Cinnamate 4-Monooxygenase.
Abstract
Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from 'not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.
PubMed: 11575728
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Betz</name><affiliation wicri:level="2"><nlm:affiliation>USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T G" last="Mccollum">T G Mccollum</name></author><author><name sortKey="Mayer, R T" sort="Mayer, R T" uniqKey="Mayer R" first="R T" last="Mayer">R T Mayer</name></author></analytic><series><title level="j">Plant molecular biology</title><idno type="ISSN">0167-4412</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus (enzymology)</term><term>Cytochrome P-450 Enzyme System (genetics)</term><term>DNA, Complementary</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Mixed Function Oxygenases (genetics)</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Trans-Cinnamate 4-Monooxygenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cytochrome P-450 Enzyme System</term><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Trans-Cinnamate 4-Monooxygenase</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from 'not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11575728</PMID><DateCreated><Year>2001</Year><Month>9</Month><Day>28</Day></DateCreated><DateCompleted><Year>2001</Year><Month>10</Month><Day>18</Day></DateCompleted><DateRevised><Year>2005</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0167-4412</ISSN><JournalIssue CitedMedium="Print"><Volume>46</Volume><Issue>6</Issue><PubDate><Year>2001</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant molecular biology</Title><ISOAbbreviation>Plant Mol. Biol.</ISOAbbreviation></Journal><ArticleTitle>Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).</ArticleTitle><Pagination><MedlinePgn>741-8</MedlinePgn></Pagination><Abstract><AbstractText>Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from 'not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Betz</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McCollum</LastName><ForeName>T G</ForeName><Initials>TG</Initials></Author><Author ValidYN="Y"><LastName>Mayer</LastName><ForeName>R T</ForeName><Initials>RT</Initials></Author></AuthorList><Language>ENG</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AF255013</AccessionNumber><AccessionNumber>AF255014</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Plant Mol Biol</MedlineTA><NlmUniqueID>9106343</NlmUniqueID><ISSNLinking>0167-4412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>9035-51-2</RegistryNumber><NameOfSubstance UI="D003577">Cytochrome P-450 Enzyme System</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.13.11</RegistryNumber><NameOfSubstance UI="D050564">Trans-Cinnamate 4-Monooxygenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002957" MajorTopicYN="N">Citrus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003577" MajorTopicYN="N">Cytochrome P-450 Enzyme System</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="Y">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050564" MajorTopicYN="N">Trans-Cinnamate 4-Monooxygenase</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>9</Month><Day>29</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>10</Month><Day>19</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>9</Month><Day>29</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11575728</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><noCountry><name sortKey="Mayer, R T" sort="Mayer, R T" uniqKey="Mayer R" first="R T" last="Mayer">R T Mayer</name><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T G" last="Mccollum">T G Mccollum</name></noCountry><country name="États-Unis"><region name="Floride"><name sortKey="Betz, C" sort="Betz, C" uniqKey="Betz C" first="C" last="Betz">C. 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