The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit.
Identifieur interne : 000B44 ( PubMed/Checkpoint ); précédent : 000B43; suivant : 000B45The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit.
Auteurs : Masaya Kato [Japon] ; Hikaru Matsumoto ; Yoshinori Ikoma ; Hitoshi Okuda ; Masamichi YanoSource :
- Journal of experimental botany [ 0022-0957 ] ; 2006.
English descriptors
- KwdEn :
- Abscisic Acid (metabolism), Carotenoids (metabolism), Citrus (genetics), Citrus (growth & development), Citrus (metabolism), DNA, Complementary (chemistry), Dioxygenases (metabolism), Fruit (growth & development), Fruit (metabolism), Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Genes, Plant, Oxygenases (metabolism), Plant Proteins, Recombinant Proteins (chemistry), Xanthophylls (metabolism).
- MESH :
- chemical , chemistry : DNA, Complementary, Recombinant Proteins.
- chemical , metabolism : Abscisic Acid, Carotenoids, Dioxygenases, Oxygenases, Xanthophylls.
- genetics : Citrus.
- growth & development : Citrus, Fruit.
- metabolism : Citrus, Fruit.
- Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Genes, Plant, Plant Proteins.
Abstract
To investigate the relationship between a carotenoid profile and gene expression for carotenoid cleavage dioxygenases, three citrus varieties that exhibit different 9-cis-violaxanthin levels in their juice sacs, Satsuma mandarin (Citrus unshiu Marc.; a variety accumulating a low level of 9-cis-violaxanthin), Valencia orange (Citrus sinensis Osbeck; variety accumulating a high level of 9-cis-violaxanthin), and Lisbon lemon (Citrus limon Burm.f.; a variety accumulating an undetectable level of 9-cis-violaxanthin) were used. Three cDNAs (CitCCD1, CitNCED2, and CitNCED3) were cloned. The recombinant CitCCD1 protein cleaved beta-cryptoxanthin, zeaxanthin, and all-trans-violaxanthin at the 9-10 and 9'-10' positions and 9-cis-violaxanthin at the 9'-10' position. The recombinant CitNCED2 and CitNCED3 proteins cleaved 9-cis-violaxanthin at the 11-12 position to form xanthoxin, a precursor of abscisic acid (ABA). The gene expression of CitCCD1 increased in the flavedos and juice sacs of the three varieties during maturation. In Satsuma mandarin, the gene expression of CitNCED2 and CitNCED3 increased noticeably, accompanying a massive accumulation of ABA in the flavedo and juice sacs. In Valencia orange, the gene expression of CitNCED3 increased with a slight elevation of the ABA level in the flavedo, whereas neither the gene expression of CitNCED2 nor the ABA level increased noticeably in the juice sacs. In Lisbon lemon, the gene expression of CitNCED2 increased remarkably, accompanying increases in the ABA level in the flavedo and juice sacs. These results suggest that, in the juice sacs, the efficient cleavage reaction for ABA synthesis reduces the 9-cis-violaxanthin level in Satsuma mandarin and Lisbon lemon, whereas the low cleavage reaction maintains the predominant 9-cis-violaxanthin accumulation in Valencia orange.
DOI: 10.1093/jxb/erj172
PubMed: 16714310
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit.</title><author><name sortKey="Kato, Masaya" sort="Kato, Masaya" uniqKey="Kato M" first="Masaya" last="Kato">Masaya Kato</name><affiliation wicri:level="1"><nlm:affiliation>Department of Citrus Research, National Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan. masayaka@agr.shizuoka.ac.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Citrus Research, National Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292</wicri:regionArea><wicri:noRegion>Shizuoka 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Matsumoto, Hikaru" sort="Matsumoto, Hikaru" uniqKey="Matsumoto H" first="Hikaru" last="Matsumoto">Hikaru Matsumoto</name></author><author><name sortKey="Ikoma, Yoshinori" sort="Ikoma, Yoshinori" uniqKey="Ikoma Y" first="Yoshinori" last="Ikoma">Yoshinori Ikoma</name></author><author><name sortKey="Okuda, Hitoshi" sort="Okuda, Hitoshi" uniqKey="Okuda H" first="Hitoshi" last="Okuda">Hitoshi Okuda</name></author><author><name sortKey="Yano, Masamichi" sort="Yano, Masamichi" uniqKey="Yano M" first="Masamichi" last="Yano">Masamichi Yano</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16714310</idno><idno type="pmid">16714310</idno><idno type="doi">10.1093/jxb/erj172</idno><idno type="wicri:Area/PubMed/Corpus">000C49</idno><idno type="wicri:Area/PubMed/Curation">000C49</idno><idno type="wicri:Area/PubMed/Checkpoint">000C49</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit.</title><author><name sortKey="Kato, Masaya" sort="Kato, Masaya" uniqKey="Kato M" first="Masaya" last="Kato">Masaya Kato</name><affiliation wicri:level="1"><nlm:affiliation>Department of Citrus Research, National Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan. masayaka@agr.shizuoka.ac.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Citrus Research, National Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292</wicri:regionArea><wicri:noRegion>Shizuoka 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Matsumoto, Hikaru" sort="Matsumoto, Hikaru" uniqKey="Matsumoto H" first="Hikaru" last="Matsumoto">Hikaru Matsumoto</name></author><author><name sortKey="Ikoma, Yoshinori" sort="Ikoma, Yoshinori" uniqKey="Ikoma Y" first="Yoshinori" last="Ikoma">Yoshinori Ikoma</name></author><author><name sortKey="Okuda, Hitoshi" sort="Okuda, Hitoshi" uniqKey="Okuda H" first="Hitoshi" last="Okuda">Hitoshi Okuda</name></author><author><name sortKey="Yano, Masamichi" sort="Yano, Masamichi" uniqKey="Yano M" first="Masamichi" last="Yano">Masamichi Yano</name></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="ISSN">0022-0957</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic Acid (metabolism)</term><term>Carotenoids (metabolism)</term><term>Citrus (genetics)</term><term>Citrus (growth & development)</term><term>Citrus (metabolism)</term><term>DNA, Complementary (chemistry)</term><term>Dioxygenases (metabolism)</term><term>Fruit (growth & development)</term><term>Fruit (metabolism)</term><term>Gene Expression Regulation, Developmental</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Oxygenases (metabolism)</term><term>Plant Proteins</term><term>Recombinant Proteins (chemistry)</term><term>Xanthophylls (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Abscisic Acid</term><term>Carotenoids</term><term>Dioxygenases</term><term>Oxygenases</term><term>Xanthophylls</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Citrus</term><term>Fruit</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus</term><term>Fruit</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Developmental</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Plant Proteins</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To investigate the relationship between a carotenoid profile and gene expression for carotenoid cleavage dioxygenases, three citrus varieties that exhibit different 9-cis-violaxanthin levels in their juice sacs, Satsuma mandarin (Citrus unshiu Marc.; a variety accumulating a low level of 9-cis-violaxanthin), Valencia orange (Citrus sinensis Osbeck; variety accumulating a high level of 9-cis-violaxanthin), and Lisbon lemon (Citrus limon Burm.f.; a variety accumulating an undetectable level of 9-cis-violaxanthin) were used. Three cDNAs (CitCCD1, CitNCED2, and CitNCED3) were cloned. The recombinant CitCCD1 protein cleaved beta-cryptoxanthin, zeaxanthin, and all-trans-violaxanthin at the 9-10 and 9'-10' positions and 9-cis-violaxanthin at the 9'-10' position. The recombinant CitNCED2 and CitNCED3 proteins cleaved 9-cis-violaxanthin at the 11-12 position to form xanthoxin, a precursor of abscisic acid (ABA). The gene expression of CitCCD1 increased in the flavedos and juice sacs of the three varieties during maturation. In Satsuma mandarin, the gene expression of CitNCED2 and CitNCED3 increased noticeably, accompanying a massive accumulation of ABA in the flavedo and juice sacs. In Valencia orange, the gene expression of CitNCED3 increased with a slight elevation of the ABA level in the flavedo, whereas neither the gene expression of CitNCED2 nor the ABA level increased noticeably in the juice sacs. In Lisbon lemon, the gene expression of CitNCED2 increased remarkably, accompanying increases in the ABA level in the flavedo and juice sacs. These results suggest that, in the juice sacs, the efficient cleavage reaction for ABA synthesis reduces the 9-cis-violaxanthin level in Satsuma mandarin and Lisbon lemon, whereas the low cleavage reaction maintains the predominant 9-cis-violaxanthin accumulation in Valencia orange.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16714310</PMID><DateCreated><Year>2006</Year><Month>7</Month><Day>11</Day></DateCreated><DateCompleted><Year>2006</Year><Month>10</Month><Day>25</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0022-0957</ISSN><JournalIssue CitedMedium="Print"><Volume>57</Volume><Issue>10</Issue><PubDate><Year>2006</Year></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J. Exp. Bot.</ISOAbbreviation></Journal><ArticleTitle>The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit.</ArticleTitle><Pagination><MedlinePgn>2153-64</MedlinePgn></Pagination><Abstract><AbstractText>To investigate the relationship between a carotenoid profile and gene expression for carotenoid cleavage dioxygenases, three citrus varieties that exhibit different 9-cis-violaxanthin levels in their juice sacs, Satsuma mandarin (Citrus unshiu Marc.; a variety accumulating a low level of 9-cis-violaxanthin), Valencia orange (Citrus sinensis Osbeck; variety accumulating a high level of 9-cis-violaxanthin), and Lisbon lemon (Citrus limon Burm.f.; a variety accumulating an undetectable level of 9-cis-violaxanthin) were used. Three cDNAs (CitCCD1, CitNCED2, and CitNCED3) were cloned. The recombinant CitCCD1 protein cleaved beta-cryptoxanthin, zeaxanthin, and all-trans-violaxanthin at the 9-10 and 9'-10' positions and 9-cis-violaxanthin at the 9'-10' position. The recombinant CitNCED2 and CitNCED3 proteins cleaved 9-cis-violaxanthin at the 11-12 position to form xanthoxin, a precursor of abscisic acid (ABA). The gene expression of CitCCD1 increased in the flavedos and juice sacs of the three varieties during maturation. In Satsuma mandarin, the gene expression of CitNCED2 and CitNCED3 increased noticeably, accompanying a massive accumulation of ABA in the flavedo and juice sacs. In Valencia orange, the gene expression of CitNCED3 increased with a slight elevation of the ABA level in the flavedo, whereas neither the gene expression of CitNCED2 nor the ABA level increased noticeably in the juice sacs. In Lisbon lemon, the gene expression of CitNCED2 increased remarkably, accompanying increases in the ABA level in the flavedo and juice sacs. These results suggest that, in the juice sacs, the efficient cleavage reaction for ABA synthesis reduces the 9-cis-violaxanthin level in Satsuma mandarin and Lisbon lemon, whereas the low cleavage reaction maintains the predominant 9-cis-violaxanthin accumulation in Valencia orange.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kato</LastName><ForeName>Masaya</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Citrus Research, National Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan. masayaka@agr.shizuoka.ac.jp</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Matsumoto</LastName><ForeName>Hikaru</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Ikoma</LastName><ForeName>Yoshinori</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Okuda</LastName><ForeName>Hitoshi</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Yano</LastName><ForeName>Masamichi</ForeName><Initials>M</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>May</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D024341">Xanthophylls</NameOfSubstance></Chemical><Chemical><RegistryNumber>36-88-4</RegistryNumber><NameOfSubstance UI="D002338">Carotenoids</NameOfSubstance></Chemical><Chemical><RegistryNumber>51C926029A</RegistryNumber><NameOfSubstance UI="C005613">violaxanthin</NameOfSubstance></Chemical><Chemical><RegistryNumber>72S9A8J5GW</RegistryNumber><NameOfSubstance UI="D000040">Abscisic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.-</RegistryNumber><NameOfSubstance UI="D010105">Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.11.-</RegistryNumber><NameOfSubstance UI="D049308">Dioxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.11.-</RegistryNumber><NameOfSubstance UI="C431939">carotenoid cleavage dioxygenase 1</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.11.51</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049308" MajorTopicYN="N">Dioxygenases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018507" MajorTopicYN="N">Gene Expression Regulation, Developmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010105" MajorTopicYN="N">Oxygenases</DescriptorName><QualifierName UI="Q000378" 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PubStatus="entrez"><Year>2006</Year><Month>5</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16714310</ArticleId><ArticleId IdType="pii">erj172</ArticleId><ArticleId IdType="doi">10.1093/jxb/erj172</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Ikoma, Yoshinori" sort="Ikoma, Yoshinori" uniqKey="Ikoma Y" first="Yoshinori" last="Ikoma">Yoshinori Ikoma</name><name sortKey="Matsumoto, Hikaru" sort="Matsumoto, Hikaru" uniqKey="Matsumoto H" first="Hikaru" last="Matsumoto">Hikaru Matsumoto</name><name sortKey="Okuda, Hitoshi" sort="Okuda, Hitoshi" uniqKey="Okuda H" first="Hitoshi" last="Okuda">Hitoshi Okuda</name><name sortKey="Yano, Masamichi" sort="Yano, Masamichi" uniqKey="Yano M" first="Masamichi" last="Yano">Masamichi Yano</name></noCountry><country name="Japon"><noRegion><name sortKey="Kato, Masaya" sort="Kato, Masaya" uniqKey="Kato M" first="Masaya" last="Kato">Masaya Kato</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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