The endophyte Curtobacterium flaccumfaciens reduces symptoms caused by Xylella fastidiosa in Catharanthus roseus.
Identifieur interne : 000A62 ( PubMed/Checkpoint ); précédent : 000A61; suivant : 000A63The endophyte Curtobacterium flaccumfaciens reduces symptoms caused by Xylella fastidiosa in Catharanthus roseus.
Auteurs : Paulo Teixeira Lacava [Brésil] ; Wenbin Li ; Welington Luiz Araújo ; João Lúcio Azevedo ; John Stephen HartungSource :
- Journal of microbiology (Seoul, Korea) [ 1225-8873 ] ; 2007.
English descriptors
- KwdEn :
- MESH :
- chemical : DNA Primers.
- genetics : Xylella.
- isolation & purification : Xylella.
- microbiology : Catharanthus, Plant Diseases.
- pathogenicity : Xylella.
- physiology : Micrococcaceae.
- Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
Citrus variegated chlorosis (CVC) is a disease of the sweet orange [Citrus sinensis (L.)], which is caused by Xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. Sweet orange trees have been occasionally observed to be infected by Xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. The principal endophytic bacterial species isolated from such CVC-asymptomatic citrus plants is Curtobacterium flaccumfaciens. The Madagascar periwinkle [Citrus sinensis (L.)] is a model plant which has been used to study X. fastidiosa in greenhouse environments. In order to characterize the interactions of X. fastidiosa and C. flaccumfaciens, periwinkle plants were inoculated separately with C. flaccumfaciens, X. fastidiosa, and both bacteria together. The number of flowers produced by the plants, the heights of the plants, and the exhibited disease symptoms were evaluated. PCR-primers for C. flaccumfaciens were designed in order to verify the presence of this endophytic bacterium in plant tissue, and to complement an existing assay for X. fastidiosa. These primers were capable of detecting C. flaccumfaciens in the periwinkle in the presence of X. fastidiosa. X. fastidiosa induced stunting and reduced the number of flowers produced by the periwinkle. When C. flaccumfaciens was inoculated together with X. fastidiosa, no stunting was observed. The number of flowers produced by our doubly- inoculated plants was an intermediate between the number produced by the plants inoculated with either of the bacteria separately. Our data indicate that C. flaccumfaciens interacted with X. fastidiosa in C. roseus, and reduced the severity of the disease symptoms induced by X. fastidiosa. Periwinkle is considered to be an excellent experimental system by which the interaction of C. flaccumfaciens and other endophytic bacteria with X. fastidiosa can be studied.
PubMed: 17978797
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The endophyte Curtobacterium flaccumfaciens reduces symptoms caused by Xylella fastidiosa in Catharanthus roseus.</title><author><name sortKey="Lacava, Paulo Teixeira" sort="Lacava, Paulo Teixeira" uniqKey="Lacava P" first="Paulo Teixeira" last="Lacava">Paulo Teixeira Lacava</name><affiliation wicri:level="4"><nlm:affiliation>Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz University of São Paulo, 13400-970, Piracicaba, SP, Brazil. ptlacava@esalq.usp.br</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz University of São Paulo, 13400-970, Piracicaba, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Li, Wenbin" sort="Li, Wenbin" uniqKey="Li W" first="Wenbin" last="Li">Wenbin Li</name></author><author><name sortKey="Araujo, Welington Luiz" sort="Araujo, Welington Luiz" uniqKey="Araujo W" first="Welington Luiz" last="Araújo">Welington Luiz Araújo</name></author><author><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name></author><author><name sortKey="Hartung, John Stephen" sort="Hartung, John Stephen" uniqKey="Hartung J" first="John Stephen" last="Hartung">John Stephen Hartung</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17978797</idno><idno type="pmid">17978797</idno><idno type="wicri:Area/PubMed/Corpus">000B08</idno><idno type="wicri:Area/PubMed/Curation">000B08</idno><idno type="wicri:Area/PubMed/Checkpoint">000B08</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The endophyte Curtobacterium flaccumfaciens reduces symptoms caused by Xylella fastidiosa in Catharanthus roseus.</title><author><name sortKey="Lacava, Paulo Teixeira" sort="Lacava, Paulo Teixeira" uniqKey="Lacava P" first="Paulo Teixeira" last="Lacava">Paulo Teixeira Lacava</name><affiliation wicri:level="4"><nlm:affiliation>Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz University of São Paulo, 13400-970, Piracicaba, SP, Brazil. ptlacava@esalq.usp.br</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz University of São Paulo, 13400-970, Piracicaba, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Li, Wenbin" sort="Li, Wenbin" uniqKey="Li W" first="Wenbin" last="Li">Wenbin Li</name></author><author><name sortKey="Araujo, Welington Luiz" sort="Araujo, Welington Luiz" uniqKey="Araujo W" first="Welington Luiz" last="Araújo">Welington Luiz Araújo</name></author><author><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name></author><author><name sortKey="Hartung, John Stephen" sort="Hartung, John Stephen" uniqKey="Hartung J" first="John Stephen" last="Hartung">John Stephen Hartung</name></author></analytic><series><title level="j">Journal of microbiology (Seoul, Korea)</title><idno type="ISSN">1225-8873</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Catharanthus (microbiology)</term><term>DNA Primers</term><term>Micrococcaceae (physiology)</term><term>Plant Diseases (microbiology)</term><term>Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Xylella (genetics)</term><term>Xylella (isolation & purification)</term><term>Xylella (pathogenicity)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Xylella</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Xylella</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Catharanthus</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Xylella</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Micrococcaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus variegated chlorosis (CVC) is a disease of the sweet orange [Citrus sinensis (L.)], which is caused by Xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. Sweet orange trees have been occasionally observed to be infected by Xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. The principal endophytic bacterial species isolated from such CVC-asymptomatic citrus plants is Curtobacterium flaccumfaciens. The Madagascar periwinkle [Citrus sinensis (L.)] is a model plant which has been used to study X. fastidiosa in greenhouse environments. In order to characterize the interactions of X. fastidiosa and C. flaccumfaciens, periwinkle plants were inoculated separately with C. flaccumfaciens, X. fastidiosa, and both bacteria together. The number of flowers produced by the plants, the heights of the plants, and the exhibited disease symptoms were evaluated. PCR-primers for C. flaccumfaciens were designed in order to verify the presence of this endophytic bacterium in plant tissue, and to complement an existing assay for X. fastidiosa. These primers were capable of detecting C. flaccumfaciens in the periwinkle in the presence of X. fastidiosa. X. fastidiosa induced stunting and reduced the number of flowers produced by the periwinkle. When C. flaccumfaciens was inoculated together with X. fastidiosa, no stunting was observed. The number of flowers produced by our doubly- inoculated plants was an intermediate between the number produced by the plants inoculated with either of the bacteria separately. Our data indicate that C. flaccumfaciens interacted with X. fastidiosa in C. roseus, and reduced the severity of the disease symptoms induced by X. fastidiosa. Periwinkle is considered to be an excellent experimental system by which the interaction of C. flaccumfaciens and other endophytic bacteria with X. fastidiosa can be studied.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17978797</PMID><DateCreated><Year>2007</Year><Month>11</Month><Day>5</Day></DateCreated><DateCompleted><Year>2007</Year><Month>12</Month><Day>06</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>5</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1225-8873</ISSN><JournalIssue CitedMedium="Print"><Volume>45</Volume><Issue>5</Issue><PubDate><Year>2007</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of microbiology (Seoul, Korea)</Title><ISOAbbreviation>J. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>The endophyte Curtobacterium flaccumfaciens reduces symptoms caused by Xylella fastidiosa in Catharanthus roseus.</ArticleTitle><Pagination><MedlinePgn>388-93</MedlinePgn></Pagination><Abstract><AbstractText>Citrus variegated chlorosis (CVC) is a disease of the sweet orange [Citrus sinensis (L.)], which is caused by Xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. Sweet orange trees have been occasionally observed to be infected by Xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. The principal endophytic bacterial species isolated from such CVC-asymptomatic citrus plants is Curtobacterium flaccumfaciens. The Madagascar periwinkle [Citrus sinensis (L.)] is a model plant which has been used to study X. fastidiosa in greenhouse environments. In order to characterize the interactions of X. fastidiosa and C. flaccumfaciens, periwinkle plants were inoculated separately with C. flaccumfaciens, X. fastidiosa, and both bacteria together. The number of flowers produced by the plants, the heights of the plants, and the exhibited disease symptoms were evaluated. PCR-primers for C. flaccumfaciens were designed in order to verify the presence of this endophytic bacterium in plant tissue, and to complement an existing assay for X. fastidiosa. These primers were capable of detecting C. flaccumfaciens in the periwinkle in the presence of X. fastidiosa. X. fastidiosa induced stunting and reduced the number of flowers produced by the periwinkle. When C. flaccumfaciens was inoculated together with X. fastidiosa, no stunting was observed. The number of flowers produced by our doubly- inoculated plants was an intermediate between the number produced by the plants inoculated with either of the bacteria separately. Our data indicate that C. flaccumfaciens interacted with X. fastidiosa in C. roseus, and reduced the severity of the disease symptoms induced by X. fastidiosa. Periwinkle is considered to be an excellent experimental system by which the interaction of C. flaccumfaciens and other endophytic bacteria with X. fastidiosa can be studied.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lacava</LastName><ForeName>Paulo Teixeira</ForeName><Initials>PT</Initials><AffiliationInfo><Affiliation>Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz University of São Paulo, 13400-970, Piracicaba, SP, Brazil. ptlacava@esalq.usp.br</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Wenbin</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Araújo</LastName><ForeName>Welington Luiz</ForeName><Initials>WL</Initials></Author><Author ValidYN="Y"><LastName>Azevedo</LastName><ForeName>João Lúcio</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Hartung</LastName><ForeName>John Stephen</ForeName><Initials>JS</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>J Microbiol</MedlineTA><NlmUniqueID>9703165</NlmUniqueID><ISSNLinking>1225-8873</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D030008" MajorTopicYN="N">Catharanthus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008835" MajorTopicYN="N">Micrococcaceae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044167" MajorTopicYN="N">Xylella</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>11</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>12</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>11</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17978797</ArticleId><ArticleId IdType="pii">2599</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><noCountry><name sortKey="Araujo, Welington Luiz" sort="Araujo, Welington Luiz" uniqKey="Araujo W" first="Welington Luiz" last="Araújo">Welington Luiz Araújo</name><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name><name sortKey="Hartung, John Stephen" sort="Hartung, John Stephen" uniqKey="Hartung J" first="John Stephen" last="Hartung">John Stephen Hartung</name><name sortKey="Li, Wenbin" sort="Li, Wenbin" uniqKey="Li W" first="Wenbin" last="Li">Wenbin Li</name></noCountry><country name="Brésil"><region name="État de São Paulo"><name sortKey="Lacava, Paulo Teixeira" sort="Lacava, Paulo Teixeira" uniqKey="Lacava P" first="Paulo Teixeira" last="Lacava">Paulo Teixeira Lacava</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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