Comparative proteomics of a lycopene-accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (Citrus sinensis [L.] osbeck).
Identifieur interne : 000948 ( PubMed/Checkpoint ); précédent : 000947; suivant : 000949Comparative proteomics of a lycopene-accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (Citrus sinensis [L.] osbeck).
Auteurs : Zhiyong Pan [République populaire de Chine] ; Qing Liu ; Ze Yun ; Rui Guan ; Wenfang Zeng ; Qiang Xu ; Xiuxin DengSource :
- Proteomics [ 1615-9861 ] ; 2009.
English descriptors
- KwdEn :
- Antioxidants (metabolism), Carotenoids (metabolism), Citrus sinensis (genetics), Citrus sinensis (metabolism), Fruit (drug effects), Fruit (metabolism), Gene Expression Regulation, Plant, Genes, Plant, Mutation, Oxidative Stress, Plant Proteins (analysis), Plant Proteins (metabolism), Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Transcription, Genetic, tert-Butylhydroperoxide (pharmacology).
- MESH :
- chemical , analysis : Plant Proteins.
- chemical , metabolism : Antioxidants, Carotenoids, Plant Proteins.
- drug effects : Fruit.
- genetics : Citrus sinensis.
- metabolism : Citrus sinensis, Fruit.
- chemical , pharmacology : tert-Butylhydroperoxide.
- Gene Expression Regulation, Plant, Genes, Plant, Mutation, Oxidative Stress, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Transcription, Genetic.
Abstract
A spontaneous sweet orange (Citrus sinenesis [L.] Osbeck) mutant 'Hong Anliu' is of high value due to lycopene accumulation in the pulp. In this study, we analyzed the proteomic alterations in the pulp of 'Hong Anliu' versus its wild type (WT) at four maturing stages by using 2-DE combined with MALDI-TOF-TOF MS. Among the 74 differentially expressed proteins identified, the majority are predicted to be involved in stress response, carbohydrate/energy metabolism and regulation, or protein fate, modification and degradation. Particularly, expression levels of six anti-oxidative enzymes were altered by the mutation; and assays of their respective enzymatic activities indicated an enhanced level of oxidative stress in 'Hong Anliu', implying a regulatory role of oxidative stress on carotenogenesis. This conclusion was further confirmed by our observation that treatment of fruit pulps with tert-butylhydroperoxide (a ROS progenitor) induced lycopene accumulation in 'Hong Anliu' only. Gene expression showed that genes predicted to function upstream of lycopene biosynthesis were generally upregulated in juice sacs, but downregulated in segment membranes in both 'Hong Anliu' and its WT. The result suggests an important role of post-transcriptional regulation on carotenogenesis since lycopene was induced in 'Hong Anliu' but not WT. The result also implies that carotenogenesis in juice sacs and segment membranes of citrus fruits may be regulated by different mechanisms.
DOI: 10.1002/pmic.200900092
PubMed: 19834898
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative proteomics of a lycopene-accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (Citrus sinensis [L.] osbeck).</title><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan</wicri:regionArea><wicri:noRegion>Wuhan</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Qing" sort="Liu, Qing" uniqKey="Liu Q" first="Qing" last="Liu">Qing Liu</name></author><author><name sortKey="Yun, Ze" sort="Yun, Ze" uniqKey="Yun Z" first="Ze" last="Yun">Ze Yun</name></author><author><name sortKey="Guan, Rui" sort="Guan, Rui" uniqKey="Guan R" first="Rui" last="Guan">Rui Guan</name></author><author><name sortKey="Zeng, Wenfang" sort="Zeng, Wenfang" uniqKey="Zeng W" first="Wenfang" last="Zeng">Wenfang Zeng</name></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19834898</idno><idno type="pmid">19834898</idno><idno type="doi">10.1002/pmic.200900092</idno><idno type="wicri:Area/PubMed/Corpus">000907</idno><idno type="wicri:Area/PubMed/Curation">000907</idno><idno type="wicri:Area/PubMed/Checkpoint">000907</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative proteomics of a lycopene-accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (Citrus sinensis [L.] osbeck).</title><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan</wicri:regionArea><wicri:noRegion>Wuhan</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Qing" sort="Liu, Qing" uniqKey="Liu Q" first="Qing" last="Liu">Qing Liu</name></author><author><name sortKey="Yun, Ze" sort="Yun, Ze" uniqKey="Yun Z" first="Ze" last="Yun">Ze Yun</name></author><author><name sortKey="Guan, Rui" sort="Guan, Rui" uniqKey="Guan R" first="Rui" last="Guan">Rui Guan</name></author><author><name sortKey="Zeng, Wenfang" sort="Zeng, Wenfang" uniqKey="Zeng W" first="Wenfang" last="Zeng">Wenfang Zeng</name></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name></author></analytic><series><title level="j">Proteomics</title><idno type="eISSN">1615-9861</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antioxidants (metabolism)</term><term>Carotenoids (metabolism)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (metabolism)</term><term>Fruit (drug effects)</term><term>Fruit (metabolism)</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Mutation</term><term>Oxidative Stress</term><term>Plant Proteins (analysis)</term><term>Plant Proteins (metabolism)</term><term>Proteomics</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term><term>Transcription, Genetic</term><term>tert-Butylhydroperoxide (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antioxidants</term><term>Carotenoids</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Fruit</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>tert-Butylhydroperoxide</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Mutation</term><term>Oxidative Stress</term><term>Proteomics</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term><term>Transcription, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A spontaneous sweet orange (Citrus sinenesis [L.] Osbeck) mutant 'Hong Anliu' is of high value due to lycopene accumulation in the pulp. In this study, we analyzed the proteomic alterations in the pulp of 'Hong Anliu' versus its wild type (WT) at four maturing stages by using 2-DE combined with MALDI-TOF-TOF MS. Among the 74 differentially expressed proteins identified, the majority are predicted to be involved in stress response, carbohydrate/energy metabolism and regulation, or protein fate, modification and degradation. Particularly, expression levels of six anti-oxidative enzymes were altered by the mutation; and assays of their respective enzymatic activities indicated an enhanced level of oxidative stress in 'Hong Anliu', implying a regulatory role of oxidative stress on carotenogenesis. This conclusion was further confirmed by our observation that treatment of fruit pulps with tert-butylhydroperoxide (a ROS progenitor) induced lycopene accumulation in 'Hong Anliu' only. Gene expression showed that genes predicted to function upstream of lycopene biosynthesis were generally upregulated in juice sacs, but downregulated in segment membranes in both 'Hong Anliu' and its WT. The result suggests an important role of post-transcriptional regulation on carotenogenesis since lycopene was induced in 'Hong Anliu' but not WT. The result also implies that carotenogenesis in juice sacs and segment membranes of citrus fruits may be regulated by different mechanisms.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19834898</PMID><DateCreated><Year>2009</Year><Month>12</Month><Day>22</Day></DateCreated><DateCompleted><Year>2010</Year><Month>02</Month><Day>22</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>24</Issue><PubDate><Year>2009</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>Comparative proteomics of a lycopene-accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (Citrus sinensis [L.] osbeck).</ArticleTitle><Pagination><MedlinePgn>5455-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.200900092</ELocationID><Abstract><AbstractText>A spontaneous sweet orange (Citrus sinenesis [L.] Osbeck) mutant 'Hong Anliu' is of high value due to lycopene accumulation in the pulp. In this study, we analyzed the proteomic alterations in the pulp of 'Hong Anliu' versus its wild type (WT) at four maturing stages by using 2-DE combined with MALDI-TOF-TOF MS. Among the 74 differentially expressed proteins identified, the majority are predicted to be involved in stress response, carbohydrate/energy metabolism and regulation, or protein fate, modification and degradation. Particularly, expression levels of six anti-oxidative enzymes were altered by the mutation; and assays of their respective enzymatic activities indicated an enhanced level of oxidative stress in 'Hong Anliu', implying a regulatory role of oxidative stress on carotenogenesis. This conclusion was further confirmed by our observation that treatment of fruit pulps with tert-butylhydroperoxide (a ROS progenitor) induced lycopene accumulation in 'Hong Anliu' only. Gene expression showed that genes predicted to function upstream of lycopene biosynthesis were generally upregulated in juice sacs, but downregulated in segment membranes in both 'Hong Anliu' and its WT. The result suggests an important role of post-transcriptional regulation on carotenogenesis since lycopene was induced in 'Hong Anliu' but not WT. The result also implies that carotenogenesis in juice sacs and segment membranes of citrus fruits may be regulated by different mechanisms.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Zhiyong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Qing</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Yun</LastName><ForeName>Ze</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Guan</LastName><ForeName>Rui</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Wenfang</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Xiuxin</ForeName><Initials>X</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000975">Antioxidants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>36-88-4</RegistryNumber><NameOfSubstance UI="D002338">Carotenoids</NameOfSubstance></Chemical><Chemical><RegistryNumber>955VYL842B</RegistryNumber><NameOfSubstance UI="D020122">tert-Butylhydroperoxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>SB0N2N0WV6</RegistryNumber><NameOfSubstance UI="C015329">lycopene</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000975" MajorTopicYN="N">Antioxidants</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002338" MajorTopicYN="N">Carotenoids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="Y">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020122" MajorTopicYN="N">tert-Butylhydroperoxide</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19834898</ArticleId><ArticleId IdType="doi">10.1002/pmic.200900092</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name><name sortKey="Guan, Rui" sort="Guan, Rui" uniqKey="Guan R" first="Rui" last="Guan">Rui Guan</name><name sortKey="Liu, Qing" sort="Liu, Qing" uniqKey="Liu Q" first="Qing" last="Liu">Qing Liu</name><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name><name sortKey="Yun, Ze" sort="Yun, Ze" uniqKey="Yun Z" first="Ze" last="Yun">Ze Yun</name><name sortKey="Zeng, Wenfang" sort="Zeng, Wenfang" uniqKey="Zeng W" first="Wenfang" last="Zeng">Wenfang Zeng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong 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