Structural and biochemical characteristics of citrus flowers associated with defence against a fungal pathogen.
Identifieur interne : 000277 ( PubMed/Checkpoint ); précédent : 000276; suivant : 000278Structural and biochemical characteristics of citrus flowers associated with defence against a fungal pathogen.
Auteurs : João Paulo Rodrigues Marques [Brésil] ; Lilian Amorim [Brésil] ; Geraldo José Silva-Junior [Brésil] ; Marcel Bellato Sp Sito [Brésil] ; Beatriz Appezzato-Da Gloria [Brésil]Source :
- AoB PLANTS ; 2014.
Abstract
The constitutive characters of plants can be structural or biochemical and play an important role in their defence against pathogens. Citrus postbloom fruit drop (PFD) caused by Colletotrichum spp. is one of the most important fungal diseases of citrus. The pathogen infects the flowers, leading to premature fruit drop and reducing citrus production. However, flower buds smaller than 8 mm long are usually not infected by Colletotrichum spp. Thus, this study investigated whether there are constitutive mechanisms in flower buds related to Colletotrichum spp. infection. We studied flower buds that were 2, 3, 4, 8, 12 and 15 mm long and petals, after anthesis, of sweet orange 'Valência' using light and scanning electron microscopy and histochemistry. We evaluated the effect of volatile organic compounds (VOCs) in flowers (R-limonene and linalool) on the in vitro growth of Colletotrichum acutatum. We found that the arrangement of the epidermal papillae in the petal primordia, the occurrence of prismatic crystals and the distribution of oil glands are the main differences between buds smaller than 8 mm and buds 8-15 mm long. Osmophores at the tips of petals produced and accumulated phenols, terpenes and lipophilic compounds. Flower buds smaller than 8 mm long have constitutive structural and biochemical barriers to Colletotrichum spp. infection. In addition, this is the first time that osmophores have been reported in citrus. Our study shows that natural terpenes of Citrus flowers inhibit the fungal growth in vitro, highlighting the potential use of terpenes for the chemical control of PFD in citrus.
DOI: 10.1093/aobpla/plu090
PubMed: 25535209
Affiliations:
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However, flower buds smaller than 8 mm long are usually not infected by Colletotrichum spp. Thus, this study investigated whether there are constitutive mechanisms in flower buds related to Colletotrichum spp. infection. We studied flower buds that were 2, 3, 4, 8, 12 and 15 mm long and petals, after anthesis, of sweet orange 'Valência' using light and scanning electron microscopy and histochemistry. We evaluated the effect of volatile organic compounds (VOCs) in flowers (R-limonene and linalool) on the in vitro growth of Colletotrichum acutatum. We found that the arrangement of the epidermal papillae in the petal primordia, the occurrence of prismatic crystals and the distribution of oil glands are the main differences between buds smaller than 8 mm and buds 8-15 mm long. Osmophores at the tips of petals produced and accumulated phenols, terpenes and lipophilic compounds. Flower buds smaller than 8 mm long have constitutive structural and biochemical barriers to Colletotrichum spp. infection. In addition, this is the first time that osmophores have been reported in citrus. Our study shows that natural terpenes of Citrus flowers inhibit the fungal growth in vitro, highlighting the potential use of terpenes for the chemical control of PFD in citrus.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">25535209</PMID><DateCreated><Year>2015</Year><Month>1</Month><Day>28</Day></DateCreated><DateCompleted><Year>2015</Year><Month>01</Month><Day>28</Day></DateCompleted><DateRevised><Year>2015</Year><Month>6</Month><Day>27</Day></DateRevised><Article PubModel="Electronic"><Journal><JournalIssue CitedMedium="Print"><Volume>7</Volume><PubDate><Year>2014</Year><Month>Dec</Month><Day>22</Day></PubDate></JournalIssue><Title>AoB PLANTS</Title><ISOAbbreviation>AoB Plants</ISOAbbreviation></Journal><ArticleTitle>Structural and biochemical characteristics of citrus flowers associated with defence against a fungal pathogen.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1093/aobpla/plu090</ELocationID><ELocationID EIdType="pii" ValidYN="Y">plu090</ELocationID><Abstract><AbstractText>The constitutive characters of plants can be structural or biochemical and play an important role in their defence against pathogens. Citrus postbloom fruit drop (PFD) caused by Colletotrichum spp. is one of the most important fungal diseases of citrus. The pathogen infects the flowers, leading to premature fruit drop and reducing citrus production. However, flower buds smaller than 8 mm long are usually not infected by Colletotrichum spp. Thus, this study investigated whether there are constitutive mechanisms in flower buds related to Colletotrichum spp. infection. We studied flower buds that were 2, 3, 4, 8, 12 and 15 mm long and petals, after anthesis, of sweet orange 'Valência' using light and scanning electron microscopy and histochemistry. We evaluated the effect of volatile organic compounds (VOCs) in flowers (R-limonene and linalool) on the in vitro growth of Colletotrichum acutatum. We found that the arrangement of the epidermal papillae in the petal primordia, the occurrence of prismatic crystals and the distribution of oil glands are the main differences between buds smaller than 8 mm and buds 8-15 mm long. Osmophores at the tips of petals produced and accumulated phenols, terpenes and lipophilic compounds. Flower buds smaller than 8 mm long have constitutive structural and biochemical barriers to Colletotrichum spp. infection. In addition, this is the first time that osmophores have been reported in citrus. Our study shows that natural terpenes of Citrus flowers inhibit the fungal growth in vitro, highlighting the potential use of terpenes for the chemical control of PFD in citrus.</AbstractText><CopyrightInformation>Published by Oxford University Press on behalf of the Annals of Botany Company.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Marques</LastName><ForeName>João Paulo Rodrigues</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Universidade de São Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Cx. Postal 9, CEP 13418-900, Piracicaba, SP, Brazil joaopaulormarques@yahoo.com.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Amorim</LastName><ForeName>Lilian</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Universidade de São Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Cx. Postal 9, CEP 13418-900, Piracicaba, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva-Junior</LastName><ForeName>Geraldo José</ForeName><Initials>GJ</Initials><AffiliationInfo><Affiliation>Fundo de Defesa da Citricultura - FUNDECITRUS, Av. Adhemar Pereira de Barros, 201 | CEP: 14807-040, Vila Melhado, Araraquara, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Spósito</LastName><ForeName>Marcel Bellato</ForeName><Initials>MB</Initials><AffiliationInfo><Affiliation>Universidade de São Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Cx. Postal 9, CEP 13418-900, Piracicaba, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Appezzato-da Gloria</LastName><ForeName>Beatriz</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Universidade de São Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Cx. Postal 9, CEP 13418-900, Piracicaba, SP, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>Dec</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>AoB Plants</MedlineTA><NlmUniqueID>101539425</NlmUniqueID></MedlineJournalInfo><OtherID Source="NLM">PMC4381744</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antimicrobial activity</Keyword><Keyword MajorTopicYN="N">Citrus sinensis</Keyword><Keyword MajorTopicYN="N">Colletotrichum acutatum</Keyword><Keyword MajorTopicYN="N">calcium oxalate crystals</Keyword><Keyword MajorTopicYN="N">citrus essential oils</Keyword><Keyword MajorTopicYN="N">osmophores</Keyword><Keyword MajorTopicYN="N">plant anatomy</Keyword><Keyword MajorTopicYN="N">volatile organic compounds</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>12</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>12</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>12</Month><Day>24</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25535209</ArticleId><ArticleId IdType="pii">plu090</ArticleId><ArticleId IdType="doi">10.1093/aobpla/plu090</ArticleId><ArticleId IdType="pmc">PMC4381744</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Marques, Joao Paulo Rodrigues" sort="Marques, Joao Paulo Rodrigues" uniqKey="Marques J" first="João Paulo Rodrigues" last="Marques">João Paulo Rodrigues Marques</name></region><name sortKey="Amorim, Lilian" sort="Amorim, Lilian" uniqKey="Amorim L" first="Lilian" last="Amorim">Lilian Amorim</name><name sortKey="Appezzato Da Gloria, Beatriz" sort="Appezzato Da Gloria, Beatriz" uniqKey="Appezzato Da Gloria B" first="Beatriz" last="Appezzato-Da Gloria">Beatriz Appezzato-Da Gloria</name><name sortKey="Silva Junior, Geraldo Jose" sort="Silva Junior, Geraldo Jose" uniqKey="Silva Junior G" first="Geraldo José" last="Silva-Junior">Geraldo José Silva-Junior</name><name sortKey="Sp Sito, Marcel Bellato" sort="Sp Sito, Marcel Bellato" uniqKey="Sp Sito M" first="Marcel Bellato" last="Sp Sito">Marcel Bellato Sp Sito</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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