A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh.
Identifieur interne : 000254 ( PubMed/Checkpoint ); précédent : 000253; suivant : 000255A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh.
Auteurs : Yunliu Zeng [États-Unis] ; Jiabin Du [États-Unis] ; Lun Wang [États-Unis] ; Zhiyong Pan [États-Unis] ; Qiang Xu [États-Unis] ; Shunyuan Xiao [États-Unis] ; Xiuxin Deng [États-Unis]Source :
- Plant physiology [ 1532-2548 ] ; 2015.
English descriptors
- KwdEn :
- Carotenoids (metabolism), Chloroplast Proteins (genetics), Chloroplast Proteins (metabolism), Chromatography, High Pressure Liquid, Citrus sinensis (genetics), Citrus sinensis (metabolism), Citrus sinensis (ultrastructure), Cluster Analysis, Gene Expression Regulation, Plant, Immunoblotting, Isotope Labeling (methods), Microscopy, Electron, Transmission, Plant Leaves (genetics), Plant Leaves (metabolism), Plant Leaves (ultrastructure), Plant Proteins (genetics), Plant Proteins (metabolism), Plastids (genetics), Plastids (metabolism), Plastids (ultrastructure), Proteome (classification), Proteome (genetics), Proteome (metabolism), Proteomics (methods), Reverse Transcriptase Polymerase Chain Reaction, Tandem Mass Spectrometry.
- MESH :
- chemical , classification : Proteome.
- chemical , genetics : Chloroplast Proteins, Plant Proteins, Proteome.
- chemical , metabolism : Carotenoids, Chloroplast Proteins, Plant Proteins, Proteome.
- genetics : Citrus sinensis, Plant Leaves, Plastids.
- metabolism : Citrus sinensis, Plant Leaves, Plastids.
- methods : Isotope Labeling, Proteomics.
- ultrastructure : Citrus sinensis, Plant Leaves, Plastids.
- Chromatography, High Pressure Liquid, Cluster Analysis, Gene Expression Regulation, Plant, Immunoblotting, Microscopy, Electron, Transmission, Reverse Transcriptase Polymerase Chain Reaction, Tandem Mass Spectrometry.
Abstract
Globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (Citrus sinensis) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. Subsequent isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,386 putative plastid-localized proteins, 1,016 of which were quantified by spectral counting. The iTRAQ values reflecting the expression abundance of three identified proteins were validated by immunoblotting. Based on iTRAQ data, chromoplastogenesis appeared to be associated with three major protein expression patterns: (1) marked decrease in abundance of the proteins participating in the translation machinery through ribosome assembly; (2) increase in abundance of the proteins involved in terpenoid biosynthesis (including carotenoids), stress responses (redox, ascorbate, and glutathione), and development; and (3) maintenance of the proteins for signaling and DNA and RNA. Interestingly, a strong increase in abundance of several plastoglobule-localized proteins coincided with the formation of plastoglobules in the chromoplast. The proteomic data also showed that stable functioning of protein import, suppression of ribosome assembly, and accumulation of chromoplast proteases are correlated with the amyloplast-to-chromoplast transition; thus, these processes may play a collective role in chromoplast biogenesis and differentiation. By contrast, the chromoplast senescence process was inferred to be associated with significant increases in stress response and energy supply. In conclusion, this comprehensive proteomic study identified many potentially new plastid-localized proteins and provides insights into the potential developmental and molecular mechanisms underlying chromoplast biogenesis, differentiation, and senescence in sweet orange flesh.
DOI: 10.1104/pp.15.00645
PubMed: 26056088
Affiliations:
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andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville</wicri:cityArea></affiliation></author><author><name sortKey="Du, Jiabin" sort="Du, Jiabin" uniqKey="Du J" first="Jiabin" last="Du">Jiabin Du</name><affiliation wicri:level="2"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville</wicri:cityArea></affiliation></author><author><name sortKey="Wang, Lun" sort="Wang, Lun" uniqKey="Wang L" first="Lun" last="Wang">Lun Wang</name><affiliation wicri:level="2"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville</wicri:cityArea></affiliation></author><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name><affiliation wicri:level="2"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville</wicri:cityArea></affiliation></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name><affiliation wicri:level="2"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville</wicri:cityArea></affiliation></author><author><name sortKey="Xiao, Shunyuan" sort="Xiao, Shunyuan" uniqKey="Xiao S" first="Shunyuan" last="Xiao">Shunyuan Xiao</name><affiliation wicri:level="2"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville</wicri:cityArea></affiliation></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name><affiliation wicri:level="2"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.) xxdeng@mail.hzau.edu.cn.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville</wicri:cityArea></affiliation></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carotenoids (metabolism)</term><term>Chloroplast Proteins (genetics)</term><term>Chloroplast Proteins (metabolism)</term><term>Chromatography, High Pressure Liquid</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (metabolism)</term><term>Citrus sinensis (ultrastructure)</term><term>Cluster Analysis</term><term>Gene Expression Regulation, Plant</term><term>Immunoblotting</term><term>Isotope Labeling (methods)</term><term>Microscopy, Electron, Transmission</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (ultrastructure)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plastids (genetics)</term><term>Plastids (metabolism)</term><term>Plastids (ultrastructure)</term><term>Proteome (classification)</term><term>Proteome (genetics)</term><term>Proteome (metabolism)</term><term>Proteomics (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chloroplast Proteins</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carotenoids</term><term>Chloroplast Proteins</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term><term>Plant Leaves</term><term>Plastids</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term><term>Plant Leaves</term><term>Plastids</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Isotope Labeling</term><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Citrus sinensis</term><term>Plant Leaves</term><term>Plastids</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Cluster Analysis</term><term>Gene Expression Regulation, Plant</term><term>Immunoblotting</term><term>Microscopy, Electron, Transmission</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Tandem Mass Spectrometry</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (Citrus sinensis) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. Subsequent isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,386 putative plastid-localized proteins, 1,016 of which were quantified by spectral counting. The iTRAQ values reflecting the expression abundance of three identified proteins were validated by immunoblotting. Based on iTRAQ data, chromoplastogenesis appeared to be associated with three major protein expression patterns: (1) marked decrease in abundance of the proteins participating in the translation machinery through ribosome assembly; (2) increase in abundance of the proteins involved in terpenoid biosynthesis (including carotenoids), stress responses (redox, ascorbate, and glutathione), and development; and (3) maintenance of the proteins for signaling and DNA and RNA. Interestingly, a strong increase in abundance of several plastoglobule-localized proteins coincided with the formation of plastoglobules in the chromoplast. The proteomic data also showed that stable functioning of protein import, suppression of ribosome assembly, and accumulation of chromoplast proteases are correlated with the amyloplast-to-chromoplast transition; thus, these processes may play a collective role in chromoplast biogenesis and differentiation. By contrast, the chromoplast senescence process was inferred to be associated with significant increases in stress response and energy supply. In conclusion, this comprehensive proteomic study identified many potentially new plastid-localized proteins and provides insights into the potential developmental and molecular mechanisms underlying chromoplast biogenesis, differentiation, and senescence in sweet orange flesh.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26056088</PMID><DateCreated><Year>2015</Year><Month>8</Month><Day>6</Day></DateCreated><DateCompleted><Year>2016</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2015</Year><Month>10</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>168</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol.</ISOAbbreviation></Journal><ArticleTitle>A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh.</ArticleTitle><Pagination><MedlinePgn>1648-65</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.15.00645</ELocationID><Abstract><AbstractText>Globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (Citrus sinensis) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. Subsequent isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,386 putative plastid-localized proteins, 1,016 of which were quantified by spectral counting. The iTRAQ values reflecting the expression abundance of three identified proteins were validated by immunoblotting. Based on iTRAQ data, chromoplastogenesis appeared to be associated with three major protein expression patterns: (1) marked decrease in abundance of the proteins participating in the translation machinery through ribosome assembly; (2) increase in abundance of the proteins involved in terpenoid biosynthesis (including carotenoids), stress responses (redox, ascorbate, and glutathione), and development; and (3) maintenance of the proteins for signaling and DNA and RNA. Interestingly, a strong increase in abundance of several plastoglobule-localized proteins coincided with the formation of plastoglobules in the chromoplast. The proteomic data also showed that stable functioning of protein import, suppression of ribosome assembly, and accumulation of chromoplast proteases are correlated with the amyloplast-to-chromoplast transition; thus, these processes may play a collective role in chromoplast biogenesis and differentiation. By contrast, the chromoplast senescence process was inferred to be associated with significant increases in stress response and energy supply. In conclusion, this comprehensive proteomic study identified many potentially new plastid-localized proteins and provides insights into the potential developmental and molecular mechanisms underlying chromoplast biogenesis, differentiation, and senescence in sweet orange flesh.</AbstractText><CopyrightInformation>© 2015 American Society of Plant Biologists. All Rights Reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Yunliu</ForeName><Initials>Y</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7969-6754</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Jiabin</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-3893-1065</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Lun</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Zhiyong</ForeName><Initials>Z</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-9343-3662</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1786-9696</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Shunyuan</ForeName><Initials>S</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1348-4879</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Xiuxin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); andInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.) xxdeng@mail.hzau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>Jun</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D060365">Chloroplast Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">Carotenoids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060365" MajorTopicYN="N">Chloroplast Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015151" MajorTopicYN="N">Immunoblotting</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007553" MajorTopicYN="N">Isotope Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018087" MajorTopicYN="N">Plastids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4528763</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>4</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>6</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>6</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>6</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26056088</ArticleId><ArticleId IdType="pii">pp.15.00645</ArticleId><ArticleId IdType="doi">10.1104/pp.15.00645</ArticleId><ArticleId IdType="pmc">PMC4528763</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Zeng, Yunliu" sort="Zeng, Yunliu" uniqKey="Zeng Y" first="Yunliu" last="Zeng">Yunliu Zeng</name></region><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name><name sortKey="Du, Jiabin" sort="Du, Jiabin" uniqKey="Du J" first="Jiabin" last="Du">Jiabin Du</name><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name><name sortKey="Wang, Lun" sort="Wang, Lun" uniqKey="Wang L" first="Lun" last="Wang">Lun Wang</name><name sortKey="Xiao, Shunyuan" sort="Xiao, Shunyuan" uniqKey="Xiao S" first="Shunyuan" last="Xiao">Shunyuan Xiao</name><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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