A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments
Identifieur interne : 001047 ( Pmc/Curation ); précédent : 001046; suivant : 001048A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments
Auteurs : María J. Rodrigo [Espagne] ; Berta Alquézar [Espagne] ; Enriqueta Al S [Espagne] ; Víctor Medina [Espagne] ; Lourdes Carmona [Espagne] ; Mark Bruno [Allemagne] ; Salim Al-Babili [Allemagne] ; Lorenzo Zacarías [Espagne]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2013.
Abstract
Url:
DOI: 10.1093/jxb/ert260
PubMed: 24006419
PubMed Central: 3808326
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fruit-specific apocarotenoid pigments</title>
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<author><name sortKey="Bruno, Mark" sort="Bruno, Mark" uniqKey="Bruno M" first="Mark" last="Bruno">Mark Bruno</name>
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<author><name sortKey="Al Babili, Salim" sort="Al Babili, Salim" uniqKey="Al Babili S" first="Salim" last="Al-Babili">Salim Al-Babili</name>
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<author><name sortKey="Zacarias, Lorenzo" sort="Zacarias, Lorenzo" uniqKey="Zacarias L" first="Lorenzo" last="Zacarías">Lorenzo Zacarías</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A novel carotenoid cleavage activity involved in the biosynthesis of <italic>Citrus</italic>
fruit-specific apocarotenoid pigments</title>
<author><name sortKey="Rodrigo, Maria J" sort="Rodrigo, Maria J" uniqKey="Rodrigo M" first="María J." last="Rodrigo">María J. Rodrigo</name>
<affiliation wicri:level="1"><nlm:aff id="AF0001"><institution>Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC)</institution>
,<addr-line>Av. Agustín Escardino 7, 46980 Paterna, Valencia</addr-line>
,<country>Spain</country>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Alquezar, Berta" sort="Alquezar, Berta" uniqKey="Alquezar B" first="Berta" last="Alquézar">Berta Alquézar</name>
<affiliation wicri:level="1"><nlm:aff id="AF0001"><institution>Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC)</institution>
,<addr-line>Av. Agustín Escardino 7, 46980 Paterna, Valencia</addr-line>
,<country>Spain</country>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Al S, Enriqueta" sort="Al S, Enriqueta" uniqKey="Al S E" first="Enriqueta" last="Al S">Enriqueta Al S</name>
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,<addr-line>Av. Agustín Escardino 7, 46980 Paterna, Valencia</addr-line>
,<country>Spain</country>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Medina, Victor" sort="Medina, Victor" uniqKey="Medina V" first="Víctor" last="Medina">Víctor Medina</name>
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,<addr-line>Av. Agustín Escardino 7, 46980 Paterna, Valencia</addr-line>
,<country>Spain</country>
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<country xml:lang="fr">Espagne</country>
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<author><name sortKey="Bruno, Mark" sort="Bruno, Mark" uniqKey="Bruno M" first="Mark" last="Bruno">Mark Bruno</name>
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,<addr-line>D-79104 Freiburg</addr-line>
,<country>Germany</country>
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<country xml:lang="fr">Allemagne</country>
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<author><name sortKey="Al Babili, Salim" sort="Al Babili, Salim" uniqKey="Al Babili S" first="Salim" last="Al-Babili">Salim Al-Babili</name>
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,<addr-line>D-79104 Freiburg</addr-line>
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<author><name sortKey="Zacarias, Lorenzo" sort="Zacarias, Lorenzo" uniqKey="Zacarias L" first="Lorenzo" last="Zacarías">Lorenzo Zacarías</name>
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<series><title level="j">Journal of Experimental Botany</title>
<idno type="ISSN">0022-0957</idno>
<idno type="eISSN">1460-2431</idno>
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<front><div type="abstract" xml:lang="en"><p><italic>Citrus</italic>
is the first tree crop in terms of fruit production. The colour of <italic>Citrus</italic>
fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C<sub>30</sub>
apocarotenoids, mainly β-citraurin (3-hydroxy-β-apo-8′-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and mandarins. Though carotenoid biosynthesis and its regulation have been extensively studied in <italic>Citrus</italic>
fruits, little is known about the formation of C<sub>30</sub>
apocarotenoids. The aim of this study was to the identify carotenoid cleavage enzyme(s) [CCD(s)] involved in the peel-specific C<sub>30</sub>
apocarotenoids. <italic>In silico</italic>
data mining revealed a new family of five <italic>CCD4-type</italic>
genes in <italic>Citrus</italic>
. One gene of this family, <italic>CCD4b1</italic>
, was expressed in reproductive and vegetative tissues of different <italic>Citrus</italic>
species in a pattern correlating with the accumulation of C<sub>30</sub>
apocarotenoids. Moreover, developmental processes and treatments which alter <italic>Citrus</italic>
fruit peel pigmentation led to changes of β-citraurin content and <italic>CCD4b1</italic>
transcript levels. These results point to the involvement of CCD4b1 in β-citraurin formation and indicate that the accumulation of this compound is determined by the availability of the presumed precursors zeaxanthin and β-cryptoxanthin. Functional analysis of CCD4b1 by <italic>in vitro</italic>
assays unequivocally demonstrated the asymmetric cleavage activity at the 7′,8′ double bond in zeaxanthin and β-cryptoxanthin, confirming its role in C<sub>30</sub>
apocarotenoid biosynthesis. Thus, a novel plant carotenoid cleavage activity targeting the 7′,8′ double bond of cyclic C<sub>40</sub>
carotenoids has been identified. These results suggest that the presented enzyme is responsible for the biosynthesis of C<sub>30</sub>
apocarotenoids in <italic>Citrus</italic>
which are key pigments in fruit coloration.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Exp Bot</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Exp. Bot</journal-id>
<journal-id journal-id-type="hwp">jexbot</journal-id>
<journal-id journal-id-type="publisher-id">jexbot</journal-id>
<journal-title-group><journal-title>Journal of Experimental Botany</journal-title>
</journal-title-group>
<issn pub-type="ppub">0022-0957</issn>
<issn pub-type="epub">1460-2431</issn>
<publisher><publisher-name>Oxford University Press</publisher-name>
<publisher-loc>UK</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24006419</article-id>
<article-id pub-id-type="pmc">3808326</article-id>
<article-id pub-id-type="doi">10.1093/jxb/ert260</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Paper</subject>
</subj-group>
</article-categories>
<title-group><article-title>A novel carotenoid cleavage activity involved in the biosynthesis of <italic>Citrus</italic>
fruit-specific apocarotenoid pigments</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Rodrigo</surname>
<given-names>María J.</given-names>
</name>
<xref ref-type="aff" rid="AF0001"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="c3">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Alquézar</surname>
<given-names>Berta</given-names>
</name>
<xref ref-type="aff" rid="AF0001"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="c1"><sup>†</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no"><name><surname>Alós</surname>
<given-names>Enriqueta</given-names>
</name>
<xref ref-type="aff" rid="AF0001"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no"><name><surname>Medina</surname>
<given-names>Víctor</given-names>
</name>
<xref ref-type="aff" rid="AF0001"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no"><name><surname>Carmona</surname>
<given-names>Lourdes</given-names>
</name>
<xref ref-type="aff" rid="AF0001"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no"><name><surname>Bruno</surname>
<given-names>Mark</given-names>
</name>
<xref ref-type="aff" rid="AF0002"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Al-Babili</surname>
<given-names>Salim</given-names>
</name>
<xref ref-type="aff" rid="AF0002"><sup>2</sup>
</xref>
<xref ref-type="corresp" rid="c2"><sup>‡</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no"><name><surname>Zacarías</surname>
<given-names>Lorenzo</given-names>
</name>
<xref ref-type="aff" rid="AF0001"><sup>1</sup>
</xref>
</contrib>
<aff id="AF0001"><sup>1</sup>
<institution>Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC)</institution>
,<addr-line>Av. Agustín Escardino 7, 46980 Paterna, Valencia</addr-line>
,<country>Spain</country>
</aff>
<aff id="AF0002"><sup>2</sup>
<institution>Faculty of Biology, University of Freiburg</institution>
,<addr-line>D-79104 Freiburg</addr-line>
,<country>Germany</country>
</aff>
</contrib-group>
<author-notes><corresp id="c1"><sup>†</sup>
Present address: <institution>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera</institution>
, <addr-line>Km. 4.5, 46113, Moncada, Valencia</addr-line>
, <country>Spain</country>
.</corresp>
<corresp id="c2"><sup>‡</sup>
Present address: <institution>BESE Division, King Abdullah University of Science and Technology (KAUST)</institution>
, <addr-line>Thuwal, 23955-6900</addr-line>
, <country>Kingdom of Saudi Arabia</country>
.</corresp>
<corresp id="c3">* To whom correspondence should be addressed. E-mail: <email>mjrodrigo@iata.csic.es</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>11</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>4</day>
<month>9</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>4</day>
<month>9</month>
<year>2013</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>64</volume>
<issue>14</issue>
<fpage>4461</fpage>
<lpage>4478</lpage>
<permissions><copyright-statement>© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.</copyright-statement>
<copyright-year>2013</copyright-year>
<license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by/3.0"><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">http://creativecommons.org/licenses/by/3.0/</ext-link>
), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract><p><italic>Citrus</italic>
is the first tree crop in terms of fruit production. The colour of <italic>Citrus</italic>
fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C<sub>30</sub>
apocarotenoids, mainly β-citraurin (3-hydroxy-β-apo-8′-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and mandarins. Though carotenoid biosynthesis and its regulation have been extensively studied in <italic>Citrus</italic>
fruits, little is known about the formation of C<sub>30</sub>
apocarotenoids. The aim of this study was to the identify carotenoid cleavage enzyme(s) [CCD(s)] involved in the peel-specific C<sub>30</sub>
apocarotenoids. <italic>In silico</italic>
data mining revealed a new family of five <italic>CCD4-type</italic>
genes in <italic>Citrus</italic>
. One gene of this family, <italic>CCD4b1</italic>
, was expressed in reproductive and vegetative tissues of different <italic>Citrus</italic>
species in a pattern correlating with the accumulation of C<sub>30</sub>
apocarotenoids. Moreover, developmental processes and treatments which alter <italic>Citrus</italic>
fruit peel pigmentation led to changes of β-citraurin content and <italic>CCD4b1</italic>
transcript levels. These results point to the involvement of CCD4b1 in β-citraurin formation and indicate that the accumulation of this compound is determined by the availability of the presumed precursors zeaxanthin and β-cryptoxanthin. Functional analysis of CCD4b1 by <italic>in vitro</italic>
assays unequivocally demonstrated the asymmetric cleavage activity at the 7′,8′ double bond in zeaxanthin and β-cryptoxanthin, confirming its role in C<sub>30</sub>
apocarotenoid biosynthesis. Thus, a novel plant carotenoid cleavage activity targeting the 7′,8′ double bond of cyclic C<sub>40</sub>
carotenoids has been identified. These results suggest that the presented enzyme is responsible for the biosynthesis of C<sub>30</sub>
apocarotenoids in <italic>Citrus</italic>
which are key pigments in fruit coloration.</p>
</abstract>
<kwd-group><title>Key words:</title>
<kwd>Apocarotenoid</kwd>
<kwd>carotenoid cleavage dioxygenase</kwd>
<kwd>carotenoid</kwd>
<kwd>β-citraurin</kwd>
<kwd><italic>Citrus</italic>
</kwd>
<kwd>fruit coloration.</kwd>
</kwd-group>
<counts><page-count count="18"></page-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
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