Extraction process optimization of polyphenols from Indian Citrus sinensis – as novel antiglycative agents in the management of diabetes mellitus
Identifieur interne : 000B62 ( Pmc/Curation ); précédent : 000B61; suivant : 000B63Extraction process optimization of polyphenols from Indian Citrus sinensis – as novel antiglycative agents in the management of diabetes mellitus
Auteurs : Asaithambi Shakthi Deve [Inde] ; Thiyagarajan Sathish Kumar [Inde] ; Kuppamuthu Kumaresan [Inde] ; Vinohar Stephen Rapheal [Inde]Source :
- Journal of Diabetes and Metabolic Disorders [ 2251-6581 ] ; 2014.
Abstract
Diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose level. It has become an epidemic disease in the 21st century where, India leads the world with largest number of diabetic subjects. Non-enzymatic glycosylation (glycation) is severe form of diabetes, occurs between reducing sugar and proteins which results in the formation of advanced glycation end products (AGEs) that leads to the other complicated secondary disorders. In this context,
Different extraction methods (shake flask, centrifugation and pressurized hot water) using various extractants (organic solvents, hot water and pressurized hot water) were adopted to investigate the
Pressurized hot water extraction of
The
Url:
DOI: 10.1186/2251-6581-13-11
PubMed: 24397983
PubMed Central: 3929254
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Extraction process optimization of polyphenols from Indian <italic>Citrus sinensis –</italic> as novel antiglycative agents in the management of diabetes mellitus</title><author><name sortKey="Shakthi Deve, Asaithambi" sort="Shakthi Deve, Asaithambi" uniqKey="Shakthi Deve A" first="Asaithambi" last="Shakthi Deve">Asaithambi Shakthi Deve</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu</wicri:regionArea></affiliation></author><author><name sortKey="Sathish Kumar, Thiyagarajan" sort="Sathish Kumar, Thiyagarajan" uniqKey="Sathish Kumar T" first="Thiyagarajan" last="Sathish Kumar">Thiyagarajan Sathish Kumar</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu</wicri:regionArea></affiliation></author><author><name sortKey="Kumaresan, Kuppamuthu" sort="Kumaresan, Kuppamuthu" uniqKey="Kumaresan K" first="Kuppamuthu" last="Kumaresan">Kuppamuthu Kumaresan</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu</wicri:regionArea></affiliation></author><author><name sortKey="Rapheal, Vinohar Stephen" sort="Rapheal, Vinohar Stephen" uniqKey="Rapheal V" first="Vinohar Stephen" last="Rapheal">Vinohar Stephen Rapheal</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24397983</idno><idno type="pmc">3929254</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929254</idno><idno type="RBID">PMC:3929254</idno><idno type="doi">10.1186/2251-6581-13-11</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000B63</idno><idno type="wicri:Area/Pmc/Curation">000B62</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Extraction process optimization of polyphenols from Indian <italic>Citrus sinensis –</italic> as novel antiglycative agents in the management of diabetes mellitus</title><author><name sortKey="Shakthi Deve, Asaithambi" sort="Shakthi Deve, Asaithambi" uniqKey="Shakthi Deve A" first="Asaithambi" last="Shakthi Deve">Asaithambi Shakthi Deve</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu</wicri:regionArea></affiliation></author><author><name sortKey="Sathish Kumar, Thiyagarajan" sort="Sathish Kumar, Thiyagarajan" uniqKey="Sathish Kumar T" first="Thiyagarajan" last="Sathish Kumar">Thiyagarajan Sathish Kumar</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu</wicri:regionArea></affiliation></author><author><name sortKey="Kumaresan, Kuppamuthu" sort="Kumaresan, Kuppamuthu" uniqKey="Kumaresan K" first="Kuppamuthu" last="Kumaresan">Kuppamuthu Kumaresan</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu</wicri:regionArea></affiliation></author><author><name sortKey="Rapheal, Vinohar Stephen" sort="Rapheal, Vinohar Stephen" uniqKey="Rapheal V" first="Vinohar Stephen" last="Rapheal">Vinohar Stephen Rapheal</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Diabetes and Metabolic Disorders</title><idno type="eISSN">2251-6581</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose level. It has become an epidemic disease in the 21st century where, India leads the world with largest number of diabetic subjects. Non-enzymatic glycosylation (glycation) is severe form of diabetes, occurs between reducing sugar and proteins which results in the formation of advanced glycation end products (AGEs) that leads to the other complicated secondary disorders. In this context, <italic>Mangifera indica</italic> (Mango), <italic>Syzygium cumini</italic> (Jambul), <italic>Vitis vinifera</italic> (Grapes), <italic>Citrus sinensis</italic> (Orange), <italic>Artocarpus heterophyllus</italic> (Jackfruit), <italic>Manilkara zapota</italic> (Sapodilla) seeds were evaluated for their antiglyation activity. Attempts were made to isolate the polyphenols in the seeds that have recorded the maximum activity.</p></sec><sec><title>Methods</title><p>Different extraction methods (shake flask, centrifugation and pressurized hot water) using various extractants (organic solvents, hot water and pressurized hot water) were adopted to investigate the <italic>in vitro</italic> antiglycation activity. Central composite (CCD) design based Response Surface Methodology (RSM) was espoused to optimize the extraction process of polyphenols from the fruit seeds that have recorded poor antiglycation activity. The PTLC analysis was performed to isolate the polyphenols (Flavonoids and phenolic acids) and LC-PDA-MS analysis was done for structure prediction.</p></sec><sec><title>Results</title><p>Pressurized hot water extraction of <italic>Artocarpus heterophyllus</italic> (87.52%) and <italic>Citrus sinensis</italic> seeds (74.79%) was found to possess high and low antiglycation activity, respectively. The RSM mediated optimization process adopted for the <italic>Citrus sinensis</italic> seeds have revealed that 1:15 solvent ratio (hexane to heptane), 6 minutes and 1:20 solid to liquid ratio as the optimal conditions for the extraction of polyphenols with a maximum antiglycation activity (89.79%). The LC-PDA-MS analysis of preparative thin layer chromatography (PTLC) eluates of <italic>Artocarpus heterophyllus</italic> seed has showed the presence of compounds like quercetin (301.2), 4-hydroxy phenyl acetic acid (149.0), rhamnosyl-di-hexosyl quercetin sulphate (857.6), quercetin-3-O-xyloside (428.2), rutin (613.4), diosmetin (298.1) and luteolin (283.0).</p></sec><sec><title>Conclusion</title><p>The <italic>Artocarpus heterophyllus</italic> was observed to possess a significant antiglycation activity and the activity of <italic>Citrus sinensis</italic> was improved after the optimization process, which proved that both the seeds may be used as a traditional medicine in the management of chronic diabetes mellitus.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Singh, S" uniqKey="Singh S">S Singh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bastaki, S" uniqKey="Bastaki S">S 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Diabetes Metab Disord</journal-id><journal-id journal-id-type="iso-abbrev">J Diabetes Metab Disord</journal-id><journal-title-group><journal-title>Journal of Diabetes and Metabolic Disorders</journal-title></journal-title-group><issn pub-type="epub">2251-6581</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24397983</article-id><article-id pub-id-type="pmc">3929254</article-id><article-id pub-id-type="publisher-id">2251-6581-13-11</article-id><article-id pub-id-type="doi">10.1186/2251-6581-13-11</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Extraction process optimization of polyphenols from Indian <italic>Citrus sinensis –</italic> as novel antiglycative agents in the management of diabetes mellitus</article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Shakthi Deve</surname><given-names>Asaithambi</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>pksshakthi@gmail.com</email></contrib><contrib contrib-type="author" corresp="yes" id="A2"><name><surname>Sathish kumar</surname><given-names>Thiyagarajan</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>sathishkumart29@gmail.com</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Kumaresan</surname><given-names>Kuppamuthu</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>kdpkumaresan@gmail.com</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Rapheal</surname><given-names>Vinohar Stephen</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>stevekct@gmail.com</email></contrib></contrib-group><aff id="I1"><label>1</label>Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamil Nadu, India</aff><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>7</day><month>1</month><year>2014</year></pub-date><volume>13</volume><fpage>11</fpage><lpage>11</lpage><history><date date-type="received"><day>21</day><month>6</month><year>2013</year></date><date date-type="accepted"><day>24</day><month>12</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2014 Shakthi Deve et al.; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2014</copyright-year><copyright-holder>Shakthi Deve et al.; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an open access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.jdmdonline.com/content/13/1/11"></self-uri><abstract><sec><title>Background</title><p>Diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose level. It has become an epidemic disease in the 21st century where, India leads the world with largest number of diabetic subjects. Non-enzymatic glycosylation (glycation) is severe form of diabetes, occurs between reducing sugar and proteins which results in the formation of advanced glycation end products (AGEs) that leads to the other complicated secondary disorders. In this context, <italic>Mangifera indica</italic> (Mango), <italic>Syzygium cumini</italic> (Jambul), <italic>Vitis vinifera</italic> (Grapes), <italic>Citrus sinensis</italic> (Orange), <italic>Artocarpus heterophyllus</italic> (Jackfruit), <italic>Manilkara zapota</italic> (Sapodilla) seeds were evaluated for their antiglyation activity. Attempts were made to isolate the polyphenols in the seeds that have recorded the maximum activity.</p></sec><sec><title>Methods</title><p>Different extraction methods (shake flask, centrifugation and pressurized hot water) using various extractants (organic solvents, hot water and pressurized hot water) were adopted to investigate the <italic>in vitro</italic> antiglycation activity. Central composite (CCD) design based Response Surface Methodology (RSM) was espoused to optimize the extraction process of polyphenols from the fruit seeds that have recorded poor antiglycation activity. The PTLC analysis was performed to isolate the polyphenols (Flavonoids and phenolic acids) and LC-PDA-MS analysis was done for structure prediction.</p></sec><sec><title>Results</title><p>Pressurized hot water extraction of <italic>Artocarpus heterophyllus</italic> (87.52%) and <italic>Citrus sinensis</italic> seeds (74.79%) was found to possess high and low antiglycation activity, respectively. The RSM mediated optimization process adopted for the <italic>Citrus sinensis</italic> seeds have revealed that 1:15 solvent ratio (hexane to heptane), 6 minutes and 1:20 solid to liquid ratio as the optimal conditions for the extraction of polyphenols with a maximum antiglycation activity (89.79%). The LC-PDA-MS analysis of preparative thin layer chromatography (PTLC) eluates of <italic>Artocarpus heterophyllus</italic> seed has showed the presence of compounds like quercetin (301.2), 4-hydroxy phenyl acetic acid (149.0), rhamnosyl-di-hexosyl quercetin sulphate (857.6), quercetin-3-O-xyloside (428.2), rutin (613.4), diosmetin (298.1) and luteolin (283.0).</p></sec><sec><title>Conclusion</title><p>The <italic>Artocarpus heterophyllus</italic> was observed to possess a significant antiglycation activity and the activity of <italic>Citrus sinensis</italic> was improved after the optimization process, which proved that both the seeds may be used as a traditional medicine in the management of chronic diabetes mellitus.</p></sec></abstract><kwd-group><kwd><italic>Artocarpus heterophyllus</italic></kwd><kwd><italic>Citrus sinensis</italic></kwd><kwd>Diabetes mellitus</kwd><kwd>Response surface methodology</kwd><kwd>Quercetin</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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