In silico search, characterization and validation of new EST-SSR markers in the genus Prunus
Identifieur interne : 000651 ( Pmc/Curation ); précédent : 000650; suivant : 000652In silico search, characterization and validation of new EST-SSR markers in the genus Prunus
Auteurs : Karim Sorkheh [Iran] ; Angela S. Prudencio [Espagne] ; Azim Ghebinejad [Iran] ; Mehrana Kohei Dehkordi [Iran] ; Deniz Erogul [Turquie] ; Manuel Rubio [Espagne] ; Pedro Martínez-G Mez [Espagne]Source :
- BMC Research Notes [ 1756-0500 ] ; 2016.
Abstract
Simple sequence repeats (SSRs) are defined as sequence repeat units between 1 and 6 bp that occur in both coding and non-coding regions abundant in eukaryotic genomes, which may affect the expression of genes. In this study, expressed sequence tags (ESTs) of eight
A total of 316 SSRs were identified using MISA software. Dinucleotide SSR motifs (26.31 %) were found to be the most abundant type of repeats, followed by tri- (14.58 %), tetra- (0.53 %), and penta- (0.27 %) nucleotide motifs. An attempt was made to design primer pairs for 316 identified SSRs but these were successful for only 175 SSR sequences. The positions of SSRs with respect to ORFs were detected, and annotation of sequences containing SSRs was performed to assign function to each sequence. SSRs were also characterized (in terms of position in the reference genome and associated gene) using the two available
Findings will aid analysis of functionally important molecular markers and facilitate the analysis of genetic diversity.
The online version of this article (doi:10.1186/s13104-016-2143-y) contains supplementary material, which is available to authorized users.
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DOI: 10.1186/s13104-016-2143-y
PubMed: 27389023
PubMed Central: 4937603
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In silico search, characterization and validation of new EST-SSR markers in the genus <italic>Prunus</italic></title><author><name sortKey="Sorkheh, Karim" sort="Sorkheh, Karim" uniqKey="Sorkheh K" first="Karim" last="Sorkheh">Karim Sorkheh</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz</wicri:regionArea></affiliation></author><author><name sortKey="Prudencio, Angela S" sort="Prudencio, Angela S" uniqKey="Prudencio A" first="Angela S." last="Prudencio">Angela S. Prudencio</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia</wicri:regionArea></affiliation></author><author><name sortKey="Ghebinejad, Azim" sort="Ghebinejad, Azim" uniqKey="Ghebinejad A" first="Azim" last="Ghebinejad">Azim Ghebinejad</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz</wicri:regionArea></affiliation></author><author><name sortKey="Dehkordi, Mehrana Kohei" sort="Dehkordi, Mehrana Kohei" uniqKey="Dehkordi M" first="Mehrana Kohei" last="Dehkordi">Mehrana Kohei Dehkordi</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Agronomy, Faculty of Agriculture, Payame Noor University, P.O. Box. 19395-3697, Tehran, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy, Faculty of Agriculture, Payame Noor University, P.O. Box. 19395-3697, Tehran</wicri:regionArea></affiliation></author><author><name sortKey="Erogul, Deniz" sort="Erogul, Deniz" uniqKey="Erogul D" first="Deniz" last="Erogul">Deniz Erogul</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Department of Horticulture, Faculty of Agriculture, University of Ege, Bornova, 35100 Izmir, Turkey</nlm:aff><country xml:lang="fr">Turquie</country><wicri:regionArea>Department of Horticulture, Faculty of Agriculture, University of Ege, Bornova, 35100 Izmir</wicri:regionArea></affiliation></author><author><name sortKey="Rubio, Manuel" sort="Rubio, Manuel" uniqKey="Rubio M" first="Manuel" last="Rubio">Manuel Rubio</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia</wicri:regionArea></affiliation></author><author><name sortKey="Martinez G Mez, Pedro" sort="Martinez G Mez, Pedro" uniqKey="Martinez G Mez P" first="Pedro" last="Martínez-G Mez">Pedro Martínez-G Mez</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27389023</idno><idno type="pmc">4937603</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937603</idno><idno type="RBID">PMC:4937603</idno><idno type="doi">10.1186/s13104-016-2143-y</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000652</idno><idno type="wicri:Area/Pmc/Curation">000651</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">In silico search, characterization and validation of new EST-SSR markers in the genus <italic>Prunus</italic></title><author><name sortKey="Sorkheh, Karim" sort="Sorkheh, Karim" uniqKey="Sorkheh K" first="Karim" last="Sorkheh">Karim Sorkheh</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz</wicri:regionArea></affiliation></author><author><name sortKey="Prudencio, Angela S" sort="Prudencio, Angela S" uniqKey="Prudencio A" first="Angela S." last="Prudencio">Angela S. Prudencio</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia</wicri:regionArea></affiliation></author><author><name sortKey="Ghebinejad, Azim" sort="Ghebinejad, Azim" uniqKey="Ghebinejad A" first="Azim" last="Ghebinejad">Azim Ghebinejad</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz</wicri:regionArea></affiliation></author><author><name sortKey="Dehkordi, Mehrana Kohei" sort="Dehkordi, Mehrana Kohei" uniqKey="Dehkordi M" first="Mehrana Kohei" last="Dehkordi">Mehrana Kohei Dehkordi</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Agronomy, Faculty of Agriculture, Payame Noor University, P.O. Box. 19395-3697, Tehran, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy, Faculty of Agriculture, Payame Noor University, P.O. Box. 19395-3697, Tehran</wicri:regionArea></affiliation></author><author><name sortKey="Erogul, Deniz" sort="Erogul, Deniz" uniqKey="Erogul D" first="Deniz" last="Erogul">Deniz Erogul</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Department of Horticulture, Faculty of Agriculture, University of Ege, Bornova, 35100 Izmir, Turkey</nlm:aff><country xml:lang="fr">Turquie</country><wicri:regionArea>Department of Horticulture, Faculty of Agriculture, University of Ege, Bornova, 35100 Izmir</wicri:regionArea></affiliation></author><author><name sortKey="Rubio, Manuel" sort="Rubio, Manuel" uniqKey="Rubio M" first="Manuel" last="Rubio">Manuel Rubio</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia</wicri:regionArea></affiliation></author><author><name sortKey="Martinez G Mez, Pedro" sort="Martinez G Mez, Pedro" uniqKey="Martinez G Mez P" first="Pedro" last="Martínez-G Mez">Pedro Martínez-G Mez</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Research Notes</title><idno type="eISSN">1756-0500</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Simple sequence repeats (SSRs) are defined as sequence repeat units between 1 and 6 bp that occur in both coding and non-coding regions abundant in eukaryotic genomes, which may affect the expression of genes. In this study, expressed sequence tags (ESTs) of eight <italic>Prunus</italic> species were analyzed for in silico mining of EST-SSRs, protein annotation, and open reading frames (ORFs), and the identification of codon repetitions.</p></sec><sec><title>Results</title><p>A total of 316 SSRs were identified using MISA software. Dinucleotide SSR motifs (26.31 %) were found to be the most abundant type of repeats, followed by tri- (14.58 %), tetra- (0.53 %), and penta- (0.27 %) nucleotide motifs. An attempt was made to design primer pairs for 316 identified SSRs but these were successful for only 175 SSR sequences. The positions of SSRs with respect to ORFs were detected, and annotation of sequences containing SSRs was performed to assign function to each sequence. SSRs were also characterized (in terms of position in the reference genome and associated gene) using the two available <italic>Prunus</italic> reference genomes (mei and peach). Finally, 38 SSR markers were validated across peach, almond, plum, and apricot genotypes. This validation showed a higher transferability level of EST-SSR developed in <italic>P. mume</italic> (mei) in comparison with the rest of species analyzed.</p></sec><sec><title>Conclusions</title><p>Findings will aid analysis of functionally important molecular markers and facilitate the analysis of genetic diversity.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s13104-016-2143-y) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Potter, D" uniqKey="Potter D">D Potter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kantety, Rv" uniqKey="Kantety R">RV Kantety</name></author><author><name sortKey="La Rota, M" uniqKey="La Rota M">M La Rota</name></author><author><name sortKey="Matthews, De" uniqKey="Matthews D">DE Matthews</name></author><author><name sortKey="Sorrells, Me" uniqKey="Sorrells M">ME Sorrells</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagaraj, Sh" uniqKey="Nagaraj S">SH Nagaraj</name></author><author><name sortKey="Gasser, Rb" uniqKey="Gasser R">RB Gasser</name></author><author><name sortKey="Ranganathan, S" uniqKey="Ranganathan S">S Ranganathan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamamoto, T" uniqKey="Yamamoto T">T Yamamoto</name></author><author><name sortKey="Mochida, K" uniqKey="Mochida K">K Mochida</name></author><author><name sortKey="Imai, T" uniqKey="Imai T">T Imai</name></author><author><name sortKey="Shi, Iz" uniqKey="Shi I">IZ Shi</name></author><author><name sortKey="Ogiwara, I" uniqKey="Ogiwara I">I Ogiwara</name></author><author><name sortKey="Hayashi, T" uniqKey="Hayashi T">T Hayashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vendramin, E" uniqKey="Vendramin E">E Vendramin</name></author><author><name sortKey="Dettori, Mt" uniqKey="Dettori M">MT Dettori</name></author><author><name sortKey="Giovinazzi, J" uniqKey="Giovinazzi J">J Giovinazzi</name></author><author><name sortKey="Micali, R" uniqKey="Micali R">R Micali</name></author><author><name sortKey="Quarta, R" uniqKey="Quarta R">R Quarta</name></author><author><name sortKey="Verde, I" uniqKey="Verde I">I Verde</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Decroocq, V" uniqKey="Decroocq V">V Decroocq</name></author><author><name sortKey="Fave, Mg" uniqKey="Fave M">MG Favé</name></author><author><name sortKey="Hagen, L" uniqKey="Hagen L">L Hagen</name></author><author><name sortKey="Bordenave, L" uniqKey="Bordenave L">L Bordenave</name></author><author><name sortKey="Decroocq, S" uniqKey="Decroocq S">S Decroocq</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hagen, Ls" uniqKey="Hagen L">LS Hagen</name></author><author><name sortKey="Chaib, J" uniqKey="Chaib J">J Chaib</name></author><author><name sortKey="Fad, B" uniqKey="Fad B">B Fad</name></author><author><name sortKey="Decrocq, V" uniqKey="Decrocq V">V Decrocq</name></author><author><name sortKey="Bouchet, P" uniqKey="Bouchet P">P Bouchet</name></author><author><name sortKey="Lambert, P" uniqKey="Lambert P">P Lambert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, Y" uniqKey="Xu Y">Y Xu</name></author><author><name sortKey="Ma, Rc" uniqKey="Ma R">RC Ma</name></author><author><name sortKey="Xie, H" uniqKey="Xie H">H Xie</name></author><author><name sortKey="Liu, Jt" uniqKey="Liu J">JT Liu</name></author><author><name sortKey="Cao, Mq" uniqKey="Cao M">MQ Cao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xie, H" uniqKey="Xie H">H Xie</name></author><author><name sortKey="Sui, Y" uniqKey="Sui Y">Y Sui</name></author><author><name sortKey="Chang, Fq" uniqKey="Chang F">FQ Chang</name></author><author><name sortKey="Xu, Y" uniqKey="Xu Y">Y Xu</name></author><author><name sortKey="Ma, Rc" uniqKey="Ma R">RC Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, X" uniqKey="Li X">X Li</name></author><author><name sortKey="Shangguan, L" uniqKey="Shangguan L">L Shangguan</name></author><author><name sortKey="Song, C" uniqKey="Song C">C Song</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author><author><name sortKey="Gao, Z" uniqKey="Gao Z">Z Gao</name></author><author><name sortKey="Yu, H" uniqKey="Yu H">H Yu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Yj" uniqKey="Wang Y">YJ Wang</name></author><author><name sortKey="Li, Xy" uniqKey="Li X">XY Li</name></author><author><name sortKey="Han, J" uniqKey="Han J">J Han</name></author><author><name sortKey="Fang, Wm" uniqKey="Fang W">WM Fang</name></author><author><name sortKey="Li, Xd" uniqKey="Li X">XD Li</name></author><author><name sortKey="Wang, Ss" uniqKey="Wang S">SS Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, C" uniqKey="Chen C">C Chen</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Choi, Ya" uniqKey="Choi Y">YA Choi</name></author><author><name sortKey="Huang, S" uniqKey="Huang S">S Huang</name></author><author><name sortKey="Gmitter, Fg" uniqKey="Gmitter F">FG Gmitter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, C" uniqKey="Chen C">C Chen</name></author><author><name sortKey="Bock, Ch" uniqKey="Bock C">CH Bock</name></author><author><name sortKey="Okie, Wr" uniqKey="Okie W">WR Okie</name></author><author><name sortKey="Gmitter, Fg" uniqKey="Gmitter F">FG Gmitter</name></author><author><name sortKey="Jung, S" uniqKey="Jung S">S Jung</name></author><author><name sortKey="Main, D" uniqKey="Main D">D Main</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dettori, Mt" uniqKey="Dettori M">MT Dettori</name></author><author><name sortKey="Micali, S" uniqKey="Micali S">S Micali</name></author><author><name sortKey="Giovinazzi, J" uniqKey="Giovinazzi J">J Giovinazzi</name></author><author><name sortKey="Scalabrin, S" uniqKey="Scalabrin S">S Scalabrin</name></author><author><name sortKey="Verde, I" uniqKey="Verde I">I Verde</name></author><author><name sortKey="Cipriani, G" uniqKey="Cipriani G">G Cipriani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gupta, Pk" uniqKey="Gupta P">PK Gupta</name></author><author><name sortKey="Rustgi, S" uniqKey="Rustgi S">S Rustgi</name></author><author><name sortKey="Sharma, S" uniqKey="Sharma S">S Sharma</name></author><author><name sortKey="Singh, R" uniqKey="Singh R">R Singh</name></author><author><name sortKey="Kumar, N" uniqKey="Kumar N">N Kumar</name></author><author><name sortKey="Balyan, Hs" uniqKey="Balyan H">HS Balyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, X" uniqKey="Huang X">X Huang</name></author><author><name sortKey="Madan, A" uniqKey="Madan A">A Madan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Varshney, Rk" uniqKey="Varshney R">RK Varshney</name></author><author><name sortKey="Thiel, T" uniqKey="Thiel T">T Thiel</name></author><author><name sortKey="Stein, N" uniqKey="Stein N">N Stein</name></author><author><name sortKey="Langridge, P" uniqKey="Langridge P">P Langridge</name></author><author><name sortKey="Graner, A" uniqKey="Graner A">A Graner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gupta, S" uniqKey="Gupta S">S Gupta</name></author><author><name sortKey="Shukla, R" uniqKey="Shukla R">R Shukla</name></author><author><name sortKey="Roy, S" uniqKey="Roy S">S Roy</name></author><author><name sortKey="Sen, N" uniqKey="Sen N">N Sen</name></author><author><name sortKey="Sharma, A" uniqKey="Sharma A">A Sharma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shanker, A" uniqKey="Shanker A">A Shanker</name></author><author><name sortKey="Bhargava, A" uniqKey="Bhargava A">A Bhargava</name></author><author><name sortKey="Bajpai, R" uniqKey="Bajpai R">R Bajpai</name></author><author><name sortKey="Singh, S" uniqKey="Singh S">S Singh</name></author><author><name sortKey="Srivastava, S" uniqKey="Srivastava S">S Srivastava</name></author><author><name sortKey="Sharma, V" uniqKey="Sharma V">V Sharma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poncet, V" uniqKey="Poncet V">V Poncet</name></author><author><name sortKey="Rondeau, M" uniqKey="Rondeau M">M Rondeau</name></author><author><name sortKey="Tranchant, C" uniqKey="Tranchant C">C Tranchant</name></author><author><name sortKey="Cayrel, A" uniqKey="Cayrel A">A Cayrel</name></author><author><name sortKey="Hamon, S" uniqKey="Hamon S">S Hamon</name></author><author><name sortKey="De Kochko, A" uniqKey="De Kochko A">A de Kochko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aggarwal, Rk" uniqKey="Aggarwal R">RK Aggarwal</name></author><author><name sortKey="Hendre, Ps" uniqKey="Hendre P">PS Hendre</name></author><author><name sortKey="Varshney, Rk" uniqKey="Varshney R">RK Varshney</name></author><author><name sortKey="Bhat, Pr" uniqKey="Bhat P">PR Bhat</name></author><author><name sortKey="Krishnakumar, V" uniqKey="Krishnakumar V">V Krishnakumar</name></author><author><name sortKey="Singh, L" uniqKey="Singh L">L Singh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pinto, Lr" uniqKey="Pinto L">LR Pinto</name></author><author><name sortKey="Oliveira, Km" uniqKey="Oliveira K">KM Oliveira</name></author><author><name sortKey="Ulian, Ec" uniqKey="Ulian E">EC Ulian</name></author><author><name sortKey="Garcia, Aaf" uniqKey="Garcia A">AAF Garcia</name></author><author><name sortKey="De Souza, Ap" uniqKey="De Souza A">AP de Souza</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pashley, Ch" uniqKey="Pashley C">CH Pashley</name></author><author><name sortKey="Ellis, Jr" uniqKey="Ellis J">JR Ellis</name></author><author><name sortKey="Mccauley, De" uniqKey="Mccauley D">DE McCauley</name></author><author><name sortKey="Burke, Jm" uniqKey="Burke J">JM Burke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heesacker, A" uniqKey="Heesacker A">A Heesacker</name></author><author><name sortKey="Kishore, Vk" uniqKey="Kishore V">VK Kishore</name></author><author><name sortKey="Gao, W" uniqKey="Gao W">W Gao</name></author><author><name sortKey="Tang, S" uniqKey="Tang S">S Tang</name></author><author><name sortKey="Kolkman, Jm" uniqKey="Kolkman J">JM Kolkman</name></author><author><name sortKey="Gingle, A" uniqKey="Gingle A">A Gingle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thiel, T" uniqKey="Thiel T">T Thiel</name></author><author><name sortKey="Michalek, W" uniqKey="Michalek W">W Michalek</name></author><author><name sortKey="Varshney, Rk" uniqKey="Varshney R">RK Varshney</name></author><author><name sortKey="Graner, A" uniqKey="Graner A">A Graner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, Jk" uniqKey="Yu J">JK Yu</name></author><author><name sortKey="Dake, Tm" uniqKey="Dake T">TM Dake</name></author><author><name sortKey="Singh, S" uniqKey="Singh S">S Singh</name></author><author><name sortKey="Benscher, D" uniqKey="Benscher D">D Benscher</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Gill, B" uniqKey="Gill B">B Gill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Acu A, Cv" uniqKey="Acu A C">CV Acuña</name></author><author><name sortKey="Fernandez, P" uniqKey="Fernandez P">P Fernández</name></author><author><name sortKey="Villalba, Pv" uniqKey="Villalba P">PV Villalba</name></author><author><name sortKey="Garcia, Mn" uniqKey="Garcia M">MN García</name></author><author><name sortKey="Hopp, He" uniqKey="Hopp H">HE Hopp</name></author><author><name sortKey="Poltri, Snm" uniqKey="Poltri S">SNM Poltri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berube, Y" uniqKey="Berube Y">Y Bérubé</name></author><author><name sortKey="Zhuang, J" uniqKey="Zhuang J">J Zhuang</name></author><author><name sortKey="Rungis, D" uniqKey="Rungis D">D Rungis</name></author><author><name sortKey="Ralph, S" uniqKey="Ralph S">S Ralph</name></author><author><name sortKey="Bohlmann, J" uniqKey="Bohlmann J">J Bohlmann</name></author><author><name sortKey="Ritland, K" uniqKey="Ritland K">K Ritland</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Filiz, E" uniqKey="Filiz E">E Filiz</name></author><author><name sortKey="Koc, I" uniqKey="Koc I">I Koc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, L" uniqKey="Qiu L">L Qiu</name></author><author><name sortKey="Yang, C" uniqKey="Yang C">C Yang</name></author><author><name sortKey="Tian, B" uniqKey="Tian B">B Tian</name></author><author><name sortKey="Yang, Jb" uniqKey="Yang J">JB Yang</name></author><author><name sortKey="Liu, A" uniqKey="Liu A">A Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, Jk" uniqKey="Yu J">JK Yu</name></author><author><name sortKey="Paik, H" uniqKey="Paik H">H Paik</name></author><author><name sortKey="Choi, Jp" uniqKey="Choi J">JP Choi</name></author><author><name sortKey="Han, Jh" uniqKey="Han J">JH Han</name></author><author><name sortKey="Choe, Jk" uniqKey="Choe J">JK Choe</name></author><author><name sortKey="Hur, Cg" uniqKey="Hur C">CG Hur</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Filiz, E" uniqKey="Filiz E">E Filiz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morgante, M" uniqKey="Morgante M">M Morgante</name></author><author><name sortKey="Hanafey, M" uniqKey="Hanafey M">M Hanafey</name></author><author><name sortKey="Powell, W" uniqKey="Powell W">W Powell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Andersen, Jr" uniqKey="Andersen J">JR Andersen</name></author><author><name sortKey="Lubberstedt, T" uniqKey="Lubberstedt T">T Lubberstedt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Varshney, Rk" uniqKey="Varshney R">RK Varshney</name></author><author><name sortKey="Graner, A" uniqKey="Graner A">A Graner</name></author><author><name sortKey="Sorrells, Me" uniqKey="Sorrells M">ME Sorrells</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jung, S" uniqKey="Jung S">S Jung</name></author><author><name sortKey="Jesudurai, C" uniqKey="Jesudurai C">C Jesudurai</name></author><author><name sortKey="Staton, M" uniqKey="Staton M">M Staton</name></author><author><name sortKey="Du, Z" uniqKey="Du Z">Z Du</name></author><author><name sortKey="Ficklin, S" uniqKey="Ficklin S">S Ficklin</name></author><author><name sortKey="Cho, I" uniqKey="Cho I">I Cho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bushakra, Jm" uniqKey="Bushakra J">JM Bushakra</name></author><author><name sortKey="Lewers, Ks" uniqKey="Lewers K">KS Lewers</name></author><author><name sortKey="Staton, Me" uniqKey="Staton M">ME Staton</name></author><author><name sortKey="Zhebentyayeva, T" uniqKey="Zhebentyayeva T">T Zhebentyayeva</name></author><author><name sortKey="Saski, Ca" uniqKey="Saski C">CA Saski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tripathi, Kp" uniqKey="Tripathi K">KP Tripathi</name></author><author><name sortKey="Roy, S" uniqKey="Roy S">S Roy</name></author><author><name sortKey="Khan, F" uniqKey="Khan F">F Khan</name></author><author><name sortKey="Shasany, Ak" uniqKey="Shasany A">AK Shasany</name></author><author><name sortKey="Sharma, A" uniqKey="Sharma A">A Sharma</name></author><author><name sortKey="Khanuja, Sps" uniqKey="Khanuja S">SPS Khanuja</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cardle, L" uniqKey="Cardle L">L Cardle</name></author><author><name sortKey="Ramsay, L" uniqKey="Ramsay L">L Ramsay</name></author><author><name sortKey="Milborne, D" uniqKey="Milborne D">D Milborne</name></author><author><name sortKey="Macaulay, M" uniqKey="Macaulay M">M Macaulay</name></author><author><name sortKey="Marshall, D" uniqKey="Marshall D">D Marshall</name></author><author><name sortKey="Waugh, R" uniqKey="Waugh R">R Waugh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Toth, G" uniqKey="Toth G">G Toth</name></author><author><name sortKey="Gaspari, Z" uniqKey="Gaspari Z">Z Gaspari</name></author><author><name sortKey="Jurka, J" uniqKey="Jurka J">J Jurka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melotto Passarin, Dm" uniqKey="Melotto Passarin D">DM Melotto-passarin</name></author><author><name sortKey="Tambarussi, Ev" uniqKey="Tambarussi E">EV Tambarussi</name></author><author><name sortKey="Dressano, K" uniqKey="Dressano K">K Dressano</name></author><author><name sortKey="De, Martin Vf" uniqKey="De M">Martin VF De</name></author><author><name sortKey="Carrer, H" uniqKey="Carrer H">H Carrer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tambarussi, Ev" uniqKey="Tambarussi E">EV Tambarussi</name></author><author><name sortKey="Melotto Passarin, Dm" uniqKey="Melotto Passarin D">DM Melotto-passarin</name></author><author><name sortKey="Barbosa, Al" uniqKey="Barbosa A">AL Barbosa</name></author><author><name sortKey="Brigati, Jb" uniqKey="Brigati J">JB Brigati</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palliyarakkal, Mk" uniqKey="Palliyarakkal M">MK Palliyarakkal</name></author><author><name sortKey="Ramaswamy, M" uniqKey="Ramaswamy M">M Ramaswamy</name></author><author><name sortKey="Vadivel, A" uniqKey="Vadivel A">A Vadivel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez G Mez, P" uniqKey="Martinez G Mez P">P Martínez-Gómez</name></author><author><name sortKey="Sanchez Perez, R" uniqKey="Sanchez Perez R">R Sánchez-Pérez</name></author><author><name sortKey="Rubio, M" uniqKey="Rubio M">M Rubio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Karaoglu, H" uniqKey="Karaoglu H">H Karaoglu</name></author><author><name sortKey="Lee, Cmy" uniqKey="Lee C">CMY Lee</name></author><author><name sortKey="Meyer, W" uniqKey="Meyer W">W Meyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scott, Kd" uniqKey="Scott K">KD Scott</name></author><author><name sortKey="Eggler, P" uniqKey="Eggler P">P Eggler</name></author><author><name sortKey="Seaton, F" uniqKey="Seaton F">F Seaton</name></author><author><name sortKey="Rossetto, M" uniqKey="Rossetto M">M Rossetto</name></author><author><name sortKey="Ablett, Em" uniqKey="Ablett E">EM Ablett</name></author><author><name sortKey="Lee, Ls" uniqKey="Lee L">LS Lee</name></author><author><name sortKey="Henry, Rj" uniqKey="Henry R">RJ Henry</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Varshney, Rk" uniqKey="Varshney R">RK Varshney</name></author><author><name sortKey="Kumar, A" uniqKey="Kumar A">A Kumar</name></author><author><name sortKey="Balyan, Hs" uniqKey="Balyan H">HS Balyan</name></author><author><name sortKey="Joy, Kr" uniqKey="Joy K">KR Joy</name></author><author><name sortKey="Prasad, M" uniqKey="Prasad M">M Prasad</name></author><author><name sortKey="Gupta, Pk" uniqKey="Gupta P">PK Gupta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Metzgar, D" uniqKey="Metzgar D">D Metzgar</name></author><author><name sortKey="Bytof, J" uniqKey="Bytof J">J Bytof</name></author><author><name sortKey="Wills, C" uniqKey="Wills C">C Wills</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez G Mez, P" uniqKey="Martinez G Mez P">P Martínez-Gómez</name></author><author><name sortKey="Crisosto, Ch" uniqKey="Crisosto C">CH Crisosto</name></author><author><name sortKey="Bonghi, C" uniqKey="Bonghi C">C Bonghi</name></author><author><name sortKey="Rubio, M" uniqKey="Rubio M">M Rubio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sorkheh, K" uniqKey="Sorkheh K">K Sorkheh</name></author><author><name sortKey="Malysheva Otto, V" uniqKey="Malysheva Otto V">V Malysheva-Otto</name></author><author><name sortKey="Wirthensohn, Mg" uniqKey="Wirthensohn M">MG Wirthensohn</name></author><author><name sortKey="Tarkesh Esfahani, S" uniqKey="Tarkesh Esfahani S">S Tarkesh-Esfahani</name></author><author><name sortKey="Martinez G Mez, P" uniqKey="Martinez G Mez P">P Martínez-Gómez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salazar, Ja" uniqKey="Salazar J">JA Salazar</name></author><author><name sortKey="Ruiz, D" uniqKey="Ruiz D">D Ruiz</name></author><author><name sortKey="Campoy, Ja" uniqKey="Campoy J">JA Campoy</name></author><author><name sortKey="Sanchez Perez, R" uniqKey="Sanchez Perez R">R Sánchez-Pérez</name></author><author><name sortKey="Crisosto, Ch" uniqKey="Crisosto C">CH Crisosto</name></author><author><name sortKey="Martinez Garcia, Pj" uniqKey="Martinez Garcia P">PJ Martínez-García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gonzalez, M" uniqKey="Gonzalez M">M González</name></author><author><name sortKey="Salazar, E" uniqKey="Salazar E">E Salazar</name></author><author><name sortKey="Castillo, J" uniqKey="Castillo J">J Castillo</name></author><author><name sortKey="Morales, P" uniqKey="Morales P">P Morales</name></author><author><name sortKey="Mura Jornet, I" uniqKey="Mura Jornet I">I Mura-Jornet</name></author><author><name sortKey="Maldonado, J" uniqKey="Maldonado J">J Maldonado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Whitfield, W" uniqKey="Whitfield W">W Whitfield</name></author><author><name sortKey="Band, R" uniqKey="Band R">R Band</name></author><author><name sortKey="Bonaldo, F" uniqKey="Bonaldo F">F Bonaldo</name></author><author><name sortKey="Kumar, G" uniqKey="Kumar G">G Kumar</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name></author><author><name sortKey="Pardinas, R" uniqKey="Pardinas R">R Pardinas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pertea, G" uniqKey="Pertea G">G Pertea</name></author><author><name sortKey="Huang, X" uniqKey="Huang X">X Huang</name></author><author><name sortKey="Liang, F" uniqKey="Liang F">F Liang</name></author><author><name sortKey="Antonescu, V" uniqKey="Antonescu V">V Antonescu</name></author><author><name sortKey="Sultana, R" uniqKey="Sultana R">R Sultana</name></author><author><name sortKey="Karamycheva, S" uniqKey="Karamycheva S">S Karamycheva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name></author><author><name sortKey="Chen, W" uniqKey="Chen W">W Chen</name></author><author><name sortKey="Sun, L" uniqKey="Sun L">L Sun</name></author><author><name sortKey="Zhao, F" uniqKey="Zhao F">F Zhao</name></author><author><name sortKey="Huang, B" uniqKey="Huang B">B Huang</name></author><author><name sortKey="Yang, W" uniqKey="Yang W">W Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Verde, I" uniqKey="Verde I">I Verde</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author><author><name sortKey="Scalabrin, S" uniqKey="Scalabrin S">S Scalabrin</name></author><author><name sortKey="Jung, S" uniqKey="Jung S">S Jung</name></author><author><name sortKey="Shu, S" uniqKey="Shu S">S Shu</name></author><author><name sortKey="Marroni, F" uniqKey="Marroni F">F Marroni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doyle, Jj" uniqKey="Doyle J">JJ Doyle</name></author><author><name sortKey="Doyle, Jl" uniqKey="Doyle J">JL Doyle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanchez Perez, R" uniqKey="Sanchez Perez R">R Sánchez-Pérez</name></author><author><name sortKey="Ballester, J" uniqKey="Ballester J">J Ballester</name></author><author><name sortKey="Dicenta, F" uniqKey="Dicenta F">F Dicenta</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Martinez G Mez, P" uniqKey="Martinez G Mez P">P Martínez-Gómez</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Res Notes</journal-id><journal-id journal-id-type="iso-abbrev">BMC Res Notes</journal-id><journal-title-group><journal-title>BMC Research Notes</journal-title></journal-title-group><issn pub-type="epub">1756-0500</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27389023</article-id><article-id pub-id-type="pmc">4937603</article-id><article-id pub-id-type="publisher-id">2143</article-id><article-id pub-id-type="doi">10.1186/s13104-016-2143-y</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>In silico search, characterization and validation of new EST-SSR markers in the genus <italic>Prunus</italic></article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Sorkheh</surname><given-names>Karim</given-names></name><address><email>karimsorkheh@gmail.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Prudencio</surname><given-names>Angela S.</given-names></name><address><email>asanchez@cebas.csic.es</email></address><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Ghebinejad</surname><given-names>Azim</given-names></name><address><email>azimgheibi@mihanmail.ir</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Dehkordi</surname><given-names>Mehrana Kohei</given-names></name><address><email>m.koohi@gmail.com</email></address><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author"><name><surname>Erogul</surname><given-names>Deniz</given-names></name><address><email>denizerogul@gmail.com</email></address><xref ref-type="aff" rid="Aff4"></xref></contrib><contrib contrib-type="author"><name><surname>Rubio</surname><given-names>Manuel</given-names></name><address><email>mrubio@cebas.csic.es</email></address><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Martínez-Gómez</surname><given-names>Pedro</given-names></name><address><email>pmartinez@cebas.csic.es</email></address><xref ref-type="aff" rid="Aff2"></xref></contrib><aff id="Aff1"><label></label>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box. 61355/144, Ahvaz, Iran</aff><aff id="Aff2"><label></label>Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), PO Box 164, E-30100 Espinardo-Murcia, Spain</aff><aff id="Aff3"><label></label>Department of Agronomy, Faculty of Agriculture, Payame Noor University, P.O. Box. 19395-3697, Tehran, Iran</aff><aff id="Aff4"><label></label>Department of Horticulture, Faculty of Agriculture, University of Ege, Bornova, 35100 Izmir, Turkey</aff></contrib-group><pub-date pub-type="epub"><day>7</day><month>7</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>7</day><month>7</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>9</volume><elocation-id>336</elocation-id><history><date date-type="received"><day>3</day><month>2</month><year>2016</year></date><date date-type="accepted"><day>30</day><month>6</month><year>2016</year></date></history><permissions><copyright-statement>© The Author(s) 2016</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>Simple sequence repeats (SSRs) are defined as sequence repeat units between 1 and 6 bp that occur in both coding and non-coding regions abundant in eukaryotic genomes, which may affect the expression of genes. In this study, expressed sequence tags (ESTs) of eight <italic>Prunus</italic> species were analyzed for in silico mining of EST-SSRs, protein annotation, and open reading frames (ORFs), and the identification of codon repetitions.</p></sec><sec><title>Results</title><p>A total of 316 SSRs were identified using MISA software. Dinucleotide SSR motifs (26.31 %) were found to be the most abundant type of repeats, followed by tri- (14.58 %), tetra- (0.53 %), and penta- (0.27 %) nucleotide motifs. An attempt was made to design primer pairs for 316 identified SSRs but these were successful for only 175 SSR sequences. The positions of SSRs with respect to ORFs were detected, and annotation of sequences containing SSRs was performed to assign function to each sequence. SSRs were also characterized (in terms of position in the reference genome and associated gene) using the two available <italic>Prunus</italic> reference genomes (mei and peach). Finally, 38 SSR markers were validated across peach, almond, plum, and apricot genotypes. This validation showed a higher transferability level of EST-SSR developed in <italic>P. mume</italic> (mei) in comparison with the rest of species analyzed.</p></sec><sec><title>Conclusions</title><p>Findings will aid analysis of functionally important molecular markers and facilitate the analysis of genetic diversity.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s13104-016-2143-y) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>EST-SSR</kwd><kwd>SSR mining</kwd><kwd>Functional domain marker</kwd><kwd><italic>Prunus</italic></kwd><kwd>In silico analysis</kwd><kwd>Breeding</kwd></kwd-group><funding-group><award-group><funding-source><institution>Seneca Foundation of the Region of Murcia</institution></funding-source><award-id>19879/GERM/15</award-id><principal-award-recipient><name><surname>Martínez-Gómez</surname><given-names>Pedro</given-names></name></principal-award-recipient></award-group><award-group><funding-source><institution>Spanish Ministry of Economy and Competiveness</institution></funding-source><award-id>AGL2015-68021-R</award-id><principal-award-recipient><name><surname>Rubio</surname><given-names>Manuel</given-names></name></principal-award-recipient></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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