Ectopic Expression of an Atypical Hydrophobic Group 5 LEA Protein from Wild Peanut, Arachis diogoi Confers Abiotic Stress Tolerance in Tobacco
Identifieur interne : 000378 ( Pmc/Curation ); précédent : 000377; suivant : 000379Ectopic Expression of an Atypical Hydrophobic Group 5 LEA Protein from Wild Peanut, Arachis diogoi Confers Abiotic Stress Tolerance in Tobacco
Auteurs : Akanksha Sharma [Inde] ; Dilip Kumar [Inde, Israël] ; Sumit Kumar [Inde] ; Sakshi Rampuria [Inde] ; Attipalli R. Reddy [Inde] ; Pulugurtha Bharadwaja Kirti [Inde]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
Late embryogenesis abundant (LEA) proteins are a group of hydrophilic proteins, which accumulate in plants under varied stress conditions like drought, salinity, extreme temperatures and oxidative stress suggesting their role in the protection of plants against these stresses. A transcript derived fragment (TDF) corresponding to
Url:
DOI: 10.1371/journal.pone.0150609
PubMed: 26938884
PubMed Central: 4777422
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ectopic Expression of an Atypical Hydrophobic Group 5 LEA Protein from Wild Peanut, <italic>Arachis diogoi</italic> Confers Abiotic Stress Tolerance in Tobacco</title><author><name sortKey="Sharma, Akanksha" sort="Sharma, Akanksha" uniqKey="Sharma A" first="Akanksha" last="Sharma">Akanksha Sharma</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author><author><name sortKey="Kumar, Dilip" sort="Kumar, Dilip" uniqKey="Kumar D" first="Dilip" last="Kumar">Dilip Kumar</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, Bet Dagan, 50250, Israel</addr-line></nlm:aff><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, Bet Dagan, 50250</wicri:regionArea></affiliation></author><author><name sortKey="Kumar, Sumit" sort="Kumar, Sumit" uniqKey="Kumar S" first="Sumit" last="Kumar">Sumit Kumar</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author><author><name sortKey="Rampuria, Sakshi" sort="Rampuria, Sakshi" uniqKey="Rampuria S" first="Sakshi" last="Rampuria">Sakshi Rampuria</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author><author><name sortKey="Reddy, Attipalli R" sort="Reddy, Attipalli R" uniqKey="Reddy A" first="Attipalli R." last="Reddy">Attipalli R. Reddy</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author><author><name sortKey="Kirti, Pulugurtha Bharadwaja" sort="Kirti, Pulugurtha Bharadwaja" uniqKey="Kirti P" first="Pulugurtha Bharadwaja" last="Kirti">Pulugurtha Bharadwaja Kirti</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26938884</idno><idno type="pmc">4777422</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777422</idno><idno type="RBID">PMC:4777422</idno><idno type="doi">10.1371/journal.pone.0150609</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000379</idno><idno type="wicri:Area/Pmc/Curation">000378</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Ectopic Expression of an Atypical Hydrophobic Group 5 LEA Protein from Wild Peanut, <italic>Arachis diogoi</italic> Confers Abiotic Stress Tolerance in Tobacco</title><author><name sortKey="Sharma, Akanksha" sort="Sharma, Akanksha" uniqKey="Sharma A" first="Akanksha" last="Sharma">Akanksha Sharma</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author><author><name sortKey="Kumar, Dilip" sort="Kumar, Dilip" uniqKey="Kumar D" first="Dilip" last="Kumar">Dilip Kumar</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, Bet Dagan, 50250, Israel</addr-line></nlm:aff><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, Bet Dagan, 50250</wicri:regionArea></affiliation></author><author><name sortKey="Kumar, Sumit" sort="Kumar, Sumit" uniqKey="Kumar S" first="Sumit" last="Kumar">Sumit Kumar</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author><author><name sortKey="Rampuria, Sakshi" sort="Rampuria, Sakshi" uniqKey="Rampuria S" first="Sakshi" last="Rampuria">Sakshi Rampuria</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author><author><name sortKey="Reddy, Attipalli R" sort="Reddy, Attipalli R" uniqKey="Reddy A" first="Attipalli R." last="Reddy">Attipalli R. Reddy</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author><author><name sortKey="Kirti, Pulugurtha Bharadwaja" sort="Kirti, Pulugurtha Bharadwaja" uniqKey="Kirti P" first="Pulugurtha Bharadwaja" last="Kirti">Pulugurtha Bharadwaja Kirti</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Late embryogenesis abundant (LEA) proteins are a group of hydrophilic proteins, which accumulate in plants under varied stress conditions like drought, salinity, extreme temperatures and oxidative stress suggesting their role in the protection of plants against these stresses. A transcript derived fragment (TDF) corresponding to <italic>LEA</italic> gene, which got differentially expressed in wild peanut, <italic>Arachis diogoi</italic> against the late leaf spot pathogen, <italic>Phaeoisariopsis personata</italic> was used in this study. We have cloned its full length cDNA by RACE-PCR, which was designated as <italic>AdLEA</italic>. <italic>AdLE</italic>A belongs to the atypical Group 5C of LEA protein family as confirmed by sequence analysis. Group 5C LEA protein subfamily contains Pfam LEA_2 domain and is highly hydrophobic. In native conditions, expression of <italic>AdLEA</italic> was upregulated considerably upon hormonal and abiotic stress treatments emphasizing its role in abiotic stress tolerance. Subcellular localization studies showed that AdLEA protein is distributed in both nucleus and cytosol. Ectopic expression of <italic>AdLEA</italic> in tobacco resulted in enhanced tolerance of plants to dehydration, salinity and oxidative stress with the transgenic plants showing higher chlorophyll content and reduced lipid peroxidation as compared to wild type plants. Overexpressed <italic>AdLEA</italic> tobacco plants maintained better photosynthetic efficiency under drought conditions as demonstrated by chlorophyll fluorescence measurements. These plants showed enhanced transcript accumulation of some stress-responsive genes. Our study also elucidates that ROS levels were significantly reduced in leaves and stomatal guard cells of transgenic plants upon stress treatments. These results suggest that <italic>AdLEA</italic> confers multiple stress tolerance to plants, which make it a potential gene for genetic modification in plants.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Sasaki, K" uniqKey="Sasaki K">K Sasaki</name></author><author><name sortKey="Christov, Nk" uniqKey="Christov N">NK Christov</name></author><author><name sortKey="Tsuda, S" uniqKey="Tsuda S">S Tsuda</name></author><author><name sortKey="Imai, R" uniqKey="Imai R">R Imai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dure, L" uniqKey="Dure L">L Dure</name></author><author><name sortKey="Pyle, Jb" uniqKey="Pyle J">JB Pyle</name></author><author><name sortKey="Chlan, Ca" uniqKey="Chlan C">CA Chlan</name></author><author><name sortKey="Baker, Jc" uniqKey="Baker J">JC Baker</name></author><author><name sortKey="Galau, Ga" uniqKey="Galau G">GA 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26938884</article-id><article-id pub-id-type="pmc">4777422</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0150609</article-id><article-id pub-id-type="publisher-id">PONE-D-15-44353</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Agriculture</subject><subj-group><subject>Agricultural Biotechnology</subject><subj-group><subject>Genetically Modified Organisms</subject><subj-group><subject>Genetically Modified Plants</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biotechnology</subject><subj-group><subject>Genetic Engineering</subject><subj-group><subject>Genetically Modified Organisms</subject><subj-group><subject>Genetically Modified Plants</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biotechnology</subject><subj-group><subject>Plant Biotechnology</subject><subj-group><subject>Genetically Modified Plants</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Biotechnology</subject><subj-group><subject>Genetically Modified Plants</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Plants</subject><subj-group><subject>Genetically Modified Plants</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Physiology</subject><subj-group><subject>Plant Defenses</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Pathology</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Ecology</subject><subj-group><subject>Plant Ecology</subject><subj-group><subject>Plant-Environment Interactions</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Ecology and Environmental Sciences</subject><subj-group><subject>Ecology</subject><subj-group><subject>Plant Ecology</subject><subj-group><subject>Plant-Environment Interactions</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Ecology</subject><subj-group><subject>Plant-Environment Interactions</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Anatomy</subject><subj-group><subject>Leaves</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Plants</subject><subj-group><subject>Seedlings</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Plants</subject><subj-group><subject>Flowering Plants</subject><subj-group><subject>Nicotiana</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Structures and Organelles</subject><subj-group><subject>Chloroplasts</subject><subj-group><subject>Chlorophyll</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Plant Cell Biology</subject><subj-group><subject>Chloroplasts</subject><subj-group><subject>Chlorophyll</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Cell Biology</subject><subj-group><subject>Chloroplasts</subject><subj-group><subject>Chlorophyll</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Types</subject><subj-group><subject>Plant Cells</subject><subj-group><subject>Chloroplasts</subject><subj-group><subject>Chlorophyll</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Plant Cell Biology</subject><subj-group><subject>Plant Cells</subject><subj-group><subject>Chloroplasts</subject><subj-group><subject>Chlorophyll</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Cell Biology</subject><subj-group><subject>Plant Cells</subject><subj-group><subject>Chloroplasts</subject><subj-group><subject>Chlorophyll</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Physical Sciences</subject><subj-group><subject>Materials Science</subject><subj-group><subject>Materials by Attribute</subject><subj-group><subject>Pigments</subject><subj-group><subject>Organic Pigments</subject><subj-group><subject>Chlorophyll</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Plant Biochemistry</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Biochemistry</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Molecular Biology</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Sequencing Techniques</subject><subj-group><subject>Sequence Analysis</subject><subj-group><subject>Sequence Motif Analysis</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Sequencing Techniques</subject><subj-group><subject>Sequence Analysis</subject><subj-group><subject>Sequence Motif Analysis</subject></subj-group></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Ectopic Expression of an Atypical Hydrophobic Group 5 LEA Protein from Wild Peanut, <italic>Arachis diogoi</italic> Confers Abiotic Stress Tolerance in Tobacco</article-title><alt-title alt-title-type="running-head">Group 5 LEA Confers Abiotic Stress Tolerance</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Sharma</surname><given-names>Akanksha</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib><contrib contrib-type="author"><name><surname>Kumar</surname><given-names>Dilip</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kumar</surname><given-names>Sumit</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Rampuria</surname><given-names>Sakshi</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Reddy</surname><given-names>Attipalli R.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kirti</surname><given-names>Pulugurtha Bharadwaja</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, Bet Dagan, 50250, Israel</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Ayele</surname><given-names>Belay T.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>University of Manitoba, CANADA</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: AS DK PBK. Performed the experiments: AS DK SK. Analyzed the data: AS. Contributed reagents/materials/analysis tools: SR ARR. Wrote the paper: AS.</p></fn><corresp id="cor001">* E-mail: <email>akankshasharma81@gmail.com</email></corresp></author-notes><pub-date pub-type="epub"><day>3</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>3</issue><elocation-id>e0150609</elocation-id><history><date date-type="received"><day>8</day><month>10</month><year>2015</year></date><date date-type="accepted"><day>16</day><month>2</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Sharma et al</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Sharma et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0150609.pdf"></self-uri><abstract><p>Late embryogenesis abundant (LEA) proteins are a group of hydrophilic proteins, which accumulate in plants under varied stress conditions like drought, salinity, extreme temperatures and oxidative stress suggesting their role in the protection of plants against these stresses. A transcript derived fragment (TDF) corresponding to <italic>LEA</italic> gene, which got differentially expressed in wild peanut, <italic>Arachis diogoi</italic> against the late leaf spot pathogen, <italic>Phaeoisariopsis personata</italic> was used in this study. We have cloned its full length cDNA by RACE-PCR, which was designated as <italic>AdLEA</italic>. <italic>AdLE</italic>A belongs to the atypical Group 5C of LEA protein family as confirmed by sequence analysis. Group 5C LEA protein subfamily contains Pfam LEA_2 domain and is highly hydrophobic. In native conditions, expression of <italic>AdLEA</italic> was upregulated considerably upon hormonal and abiotic stress treatments emphasizing its role in abiotic stress tolerance. Subcellular localization studies showed that AdLEA protein is distributed in both nucleus and cytosol. Ectopic expression of <italic>AdLEA</italic> in tobacco resulted in enhanced tolerance of plants to dehydration, salinity and oxidative stress with the transgenic plants showing higher chlorophyll content and reduced lipid peroxidation as compared to wild type plants. Overexpressed <italic>AdLEA</italic> tobacco plants maintained better photosynthetic efficiency under drought conditions as demonstrated by chlorophyll fluorescence measurements. These plants showed enhanced transcript accumulation of some stress-responsive genes. Our study also elucidates that ROS levels were significantly reduced in leaves and stomatal guard cells of transgenic plants upon stress treatments. These results suggest that <italic>AdLEA</italic> confers multiple stress tolerance to plants, which make it a potential gene for genetic modification in plants.</p></abstract><funding-group><funding-statement>This study was supported by funding from the Department of Biotechnology, Government of India (BT/PR/6835/PB/16/627/2005). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="11"></fig-count><table-count count="0"></table-count><page-count count="31"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information files.</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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