Potent Inhibition of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome in Bats, by Cold-Pressed, Terpeneless, Valencia Orange Oil
Identifieur interne : 000372 ( Pmc/Curation ); précédent : 000371; suivant : 000373Potent Inhibition of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome in Bats, by Cold-Pressed, Terpeneless, Valencia Orange Oil
Auteurs : Nicholas Boire [États-Unis] ; Sean Zhang [États-Unis] ; Joshua Khuvis [États-Unis] ; Rick Lee [États-Unis] ; Jennifer Rivers [États-Unis] ; Philip Crandall [États-Unis] ; M. Kevin Keel [États-Unis] ; Nicole Parrish [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
The causative agent of White-nose Syndrome (WNS),
Url:
DOI: 10.1371/journal.pone.0148473
PubMed: 26849057
PubMed Central: 4743999
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000373
Links to Exploration step
PMC:4743999Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Potent Inhibition of <italic>Pseudogymnoascus destructans</italic>
, the Causative Agent of White-Nose Syndrome in Bats, by Cold-Pressed, Terpeneless, Valencia Orange Oil</title>
<author><name sortKey="Boire, Nicholas" sort="Boire, Nicholas" uniqKey="Boire N" first="Nicholas" last="Boire">Nicholas Boire</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Sean" sort="Zhang, Sean" uniqKey="Zhang S" first="Sean" last="Zhang">Sean Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Khuvis, Joshua" sort="Khuvis, Joshua" uniqKey="Khuvis J" first="Joshua" last="Khuvis">Joshua Khuvis</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lee, Rick" sort="Lee, Rick" uniqKey="Lee R" first="Rick" last="Lee">Rick Lee</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Rivers, Jennifer" sort="Rivers, Jennifer" uniqKey="Rivers J" first="Jennifer" last="Rivers">Jennifer Rivers</name>
<affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Crandall, Philip" sort="Crandall, Philip" uniqKey="Crandall P" first="Philip" last="Crandall">Philip Crandall</name>
<affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>The University of Arkansas, Fayetteville, AR, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The University of Arkansas, Fayetteville, AR</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Keel, M Kevin" sort="Keel, M Kevin" uniqKey="Keel M" first="M. Kevin" last="Keel">M. Kevin Keel</name>
<affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>UC Davis School of Veterinary Medicine, Davis, CA, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>UC Davis School of Veterinary Medicine, Davis, CA</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Parrish, Nicole" sort="Parrish, Nicole" uniqKey="Parrish N" first="Nicole" last="Parrish">Nicole Parrish</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">26849057</idno>
<idno type="pmc">4743999</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743999</idno>
<idno type="RBID">PMC:4743999</idno>
<idno type="doi">10.1371/journal.pone.0148473</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000373</idno>
<idno type="wicri:Area/Pmc/Curation">000372</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Potent Inhibition of <italic>Pseudogymnoascus destructans</italic>
, the Causative Agent of White-Nose Syndrome in Bats, by Cold-Pressed, Terpeneless, Valencia Orange Oil</title>
<author><name sortKey="Boire, Nicholas" sort="Boire, Nicholas" uniqKey="Boire N" first="Nicholas" last="Boire">Nicholas Boire</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Sean" sort="Zhang, Sean" uniqKey="Zhang S" first="Sean" last="Zhang">Sean Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Khuvis, Joshua" sort="Khuvis, Joshua" uniqKey="Khuvis J" first="Joshua" last="Khuvis">Joshua Khuvis</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lee, Rick" sort="Lee, Rick" uniqKey="Lee R" first="Rick" last="Lee">Rick Lee</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Rivers, Jennifer" sort="Rivers, Jennifer" uniqKey="Rivers J" first="Jennifer" last="Rivers">Jennifer Rivers</name>
<affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Crandall, Philip" sort="Crandall, Philip" uniqKey="Crandall P" first="Philip" last="Crandall">Philip Crandall</name>
<affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>The University of Arkansas, Fayetteville, AR, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The University of Arkansas, Fayetteville, AR</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Keel, M Kevin" sort="Keel, M Kevin" uniqKey="Keel M" first="M. Kevin" last="Keel">M. Kevin Keel</name>
<affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>UC Davis School of Veterinary Medicine, Davis, CA, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>UC Davis School of Veterinary Medicine, Davis, CA</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Parrish, Nicole" sort="Parrish, Nicole" uniqKey="Parrish N" first="Nicole" last="Parrish">Nicole Parrish</name>
<affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Johns Hopkins School of Medicine, Baltimore, MD</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The causative agent of White-nose Syndrome (WNS), <italic>Pseudogymnoascus destructans</italic>
, has been shown to be fatal to several species of bats in North America. To date, no compounds or chemical control measures have been developed which eliminates the growth of the fungus in the environment or in affected animals. In the current study, we evaluated the activity of cold-pressed, terpeneless orange oil (CPT) against multiple isolates of <italic>P</italic>
. <italic>destructans in vitro</italic>
. For all assays, a modified Kirby-Bauer disk diffusion assay was used. Standardized spore suspensions were prepared, adjusted to a specific optical density, and used to plate fungal lawns. Plates were incubated at either 15°C or 4°C for up to 6 months and checked at regular intervals for growth. Once controls had grown, zones of inhibition were measured (mm) on test plates and compared to those obtained using current antifungal drugs. All <italic>P</italic>
. <italic>destructans</italic>
isolates were completely inhibited by 100% CPT (10 μL) at 1 month of incubation regardless of temperature (4°C and 15°C). Complete inhibition persisted up to 6 months following a single exposure at this concentration. Of the standard antifungals, only amphotericin B demonstrated any activity, resulting in zone diameters ranging from 58 mm to 74 mm. CPT, at the highest concentration tested (100%), had no significant effect against a variety of other environmental organisms including various filamentous fungi, bacteria and aerobic actinomycetes. Given that CPT is relatively non-toxic, the possibility exists that the all-natural, mixture could be used as an environmental pre-treatment to eradicate <italic>P</italic>
. <italic>destructans</italic>
from bat habitats. Additional studies are needed to assess any undesirable effects of CPT on bat behavior and health and overall impacts on other members of the interconnected ecosystem(s).</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Blehert, Ds" uniqKey="Blehert D">DS Blehert</name>
</author>
<author><name sortKey="Hicks, Ac" uniqKey="Hicks A">AC Hicks</name>
</author>
<author><name sortKey="Behr, M" uniqKey="Behr M">M Behr</name>
</author>
<author><name sortKey="Meteyer, Cu" uniqKey="Meteyer C">CU Meteyer</name>
</author>
<author><name sortKey="Berlowski Zier, Bm" uniqKey="Berlowski Zier B">BM Berlowski-Zier</name>
</author>
<author><name sortKey="Buckles, El" uniqKey="Buckles E">EL Buckles</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gargas, A" uniqKey="Gargas A">A Gargas</name>
</author>
<author><name sortKey="Trest, Mt" uniqKey="Trest M">MT Trest</name>
</author>
<author><name sortKey="Christensen, M" uniqKey="Christensen M">M Christensen</name>
</author>
<author><name sortKey="Volk, Tj" uniqKey="Volk T">TJ Volk</name>
</author>
<author><name sortKey="Blehert, Ds" uniqKey="Blehert D">DS Blehert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lorch, Jm" uniqKey="Lorch J">JM Lorch</name>
</author>
<author><name sortKey="Meteyer, Cu" uniqKey="Meteyer C">CU Meteyer</name>
</author>
<author><name sortKey="Behr, Mj" uniqKey="Behr M">MJ Behr</name>
</author>
<author><name sortKey="Boyles, Jg" uniqKey="Boyles J">JG Boyles</name>
</author>
<author><name sortKey="Cryan, Pm" uniqKey="Cryan P">PM Cryan</name>
</author>
<author><name sortKey="Hicks, Ac" uniqKey="Hicks A">AC Hicks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Warnecke, L" uniqKey="Warnecke L">L Warnecke</name>
</author>
<author><name sortKey="Turner, Jm" uniqKey="Turner J">JM Turner</name>
</author>
<author><name sortKey="Bollinger, Tk" uniqKey="Bollinger T">TK Bollinger</name>
</author>
<author><name sortKey="Lorch, Jm" uniqKey="Lorch J">JM Lorch</name>
</author>
<author><name sortKey="Misra, V" uniqKey="Misra V">V Misra</name>
</author>
<author><name sortKey="Cryan, Pm" uniqKey="Cryan P">PM Cryan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Turner, Gg" uniqKey="Turner G">GG Turner</name>
</author>
<author><name sortKey="Reeder, Dm" uniqKey="Reeder D">DM Reeder</name>
</author>
<author><name sortKey="Coleman, Jth" uniqKey="Coleman J">JTH Coleman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dzal, Y" uniqKey="Dzal Y">Y Dzal</name>
</author>
<author><name sortKey="Mcguire, Lp" uniqKey="Mcguire L">LP McGuire</name>
</author>
<author><name sortKey="Veselka, N" uniqKey="Veselka N">N Veselka</name>
</author>
<author><name sortKey="Fenton, Mb" uniqKey="Fenton M">MB Fenton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brooks, Rt" uniqKey="Brooks R">RT Brooks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blehert, Ds" uniqKey="Blehert D">DS Blehert</name>
</author>
<author><name sortKey="Lorch, Jm" uniqKey="Lorch J">JM Lorch</name>
</author>
<author><name sortKey="Ballmann, Ae" uniqKey="Ballmann A">AE Ballmann</name>
</author>
<author><name sortKey="Cryan, Pm" uniqKey="Cryan P">PM Cryan</name>
</author>
<author><name sortKey="Meteyer, Cu" uniqKey="Meteyer C">CU Meteyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Frick, Wf" uniqKey="Frick W">WF Frick</name>
</author>
<author><name sortKey="Pollock, Jf" uniqKey="Pollock J">JF Pollock</name>
</author>
<author><name sortKey="Hicks, Ac" uniqKey="Hicks A">AC Hicks</name>
</author>
<author><name sortKey="Langwig, Ke" uniqKey="Langwig K">KE Langwig</name>
</author>
<author><name sortKey="Reynolds, Ds" uniqKey="Reynolds D">DS Reynolds</name>
</author>
<author><name sortKey="Turner, Gg" uniqKey="Turner G">GG Turner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Willis, Ck" uniqKey="Willis C">CK Willis</name>
</author>
<author><name sortKey="Menzies, Ak" uniqKey="Menzies A">AK Menzies</name>
</author>
<author><name sortKey="Boyles, Jg" uniqKey="Boyles J">JG Boyles</name>
</author>
<author><name sortKey="Wojciechowski, Ms" uniqKey="Wojciechowski M">MS Wojciechowski</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boyles, Jg" uniqKey="Boyles J">JG Boyles</name>
</author>
<author><name sortKey="Willis, Ck" uniqKey="Willis C">CK Willis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Warnecke, L" uniqKey="Warnecke L">L Warnecke</name>
</author>
<author><name sortKey="Turner, Jm" uniqKey="Turner J">JM Turner</name>
</author>
<author><name sortKey="Bollinger, Tk" uniqKey="Bollinger T">TK Bollinger</name>
</author>
<author><name sortKey="Misra, V" uniqKey="Misra V">V Misra</name>
</author>
<author><name sortKey="Cryan, Pm" uniqKey="Cryan P">PM Cryan</name>
</author>
<author><name sortKey="Blehert, Ds" uniqKey="Blehert D">DS Blehert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cryan, Pm" uniqKey="Cryan P">PM Cryan</name>
</author>
<author><name sortKey="Meteyer, Cu" uniqKey="Meteyer C">CU Meteyer</name>
</author>
<author><name sortKey="Boyles, Jg" uniqKey="Boyles J">JG Boyles</name>
</author>
<author><name sortKey="Blehert, Ds" uniqKey="Blehert D">DS Blehert</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Cornelison, Ct" uniqKey="Cornelison C">CT Cornelison</name>
</author>
<author><name sortKey="Keel, Mk" uniqKey="Keel M">MK Keel</name>
</author>
<author><name sortKey="Gabriel, Kt" uniqKey="Gabriel K">KT Gabriel</name>
</author>
<author><name sortKey="Barlament, Ck" uniqKey="Barlament C">CK Barlament</name>
</author>
<author><name sortKey="Tucker, Ta" uniqKey="Tucker T">TA Tucker</name>
</author>
<author><name sortKey="Pierce, Ge" uniqKey="Pierce G">GE Pierce</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Hill, Je" uniqKey="Hill J">JE Hill</name>
</author>
<author><name sortKey="Smith, Jd" uniqKey="Smith J">JD Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boyles, Jg" uniqKey="Boyles J">JG Boyles</name>
</author>
<author><name sortKey="Cryan, Pm" uniqKey="Cryan P">PM Cryan</name>
</author>
<author><name sortKey="Mccracken, Gf" uniqKey="Mccracken G">GF McCracken</name>
</author>
<author><name sortKey="Kunz, Th" uniqKey="Kunz T">TH Kunz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chaturvedi, S" uniqKey="Chaturvedi S">S Chaturvedi</name>
</author>
<author><name sortKey="Rajkumar, Ss" uniqKey="Rajkumar S">SS Rajkumar</name>
</author>
<author><name sortKey="Li, X" uniqKey="Li X">X Li</name>
</author>
<author><name sortKey="Hurteau, Gj" uniqKey="Hurteau G">GJ Hurteau</name>
</author>
<author><name sortKey="Shtutman, M" uniqKey="Shtutman M">M Shtutman</name>
</author>
<author><name sortKey="Chaturvedi, V" uniqKey="Chaturvedi V">V Chaturvedi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muthaiyan, A" uniqKey="Muthaiyan A">A Muthaiyan</name>
</author>
<author><name sortKey="Martin, Em" uniqKey="Martin E">EM Martin</name>
</author>
<author><name sortKey="Natesan, S" uniqKey="Natesan S">S Natesan</name>
</author>
<author><name sortKey="Crandall, Pg" uniqKey="Crandall P">PG Crandall</name>
</author>
<author><name sortKey="Wilkinson, Bj" uniqKey="Wilkinson B">BJ Wilkinson</name>
</author>
<author><name sortKey="Ricke, Sc" uniqKey="Ricke S">SC Ricke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crandall, Pg" uniqKey="Crandall P">PG Crandall</name>
</author>
<author><name sortKey="Ricke, Sc" uniqKey="Ricke S">SC Ricke</name>
</author>
<author><name sortKey="O Ryan, Ca" uniqKey="O Ryan C">CA O’Bryan</name>
</author>
<author><name sortKey="Parrish, Nm" uniqKey="Parrish N">NM Parrish</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Burt, S" uniqKey="Burt S">S Burt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cox, Sd" uniqKey="Cox S">SD Cox</name>
</author>
<author><name sortKey="Gustafson, Je" uniqKey="Gustafson J">JE Gustafson</name>
</author>
<author><name sortKey="Mann, Cm" uniqKey="Mann C">CM Mann</name>
</author>
<author><name sortKey="Markham, Jl" uniqKey="Markham J">JL Markham</name>
</author>
<author><name sortKey="Liew, Yc" uniqKey="Liew Y">YC Liew</name>
</author>
<author><name sortKey="Hartland, Rp" uniqKey="Hartland R">RP Hartland</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rasooli, I" uniqKey="Rasooli I">I Rasooli</name>
</author>
<author><name sortKey="Rezaei, Mb" uniqKey="Rezaei M">MB Rezaei</name>
</author>
<author><name sortKey="Allameh, A" uniqKey="Allameh A">A Allameh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fisher, K" uniqKey="Fisher K">K Fisher</name>
</author>
<author><name sortKey="Phillips, Ca" uniqKey="Phillips C">CA Phillips</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Isman, Mb" uniqKey="Isman M">MB Isman</name>
</author>
<author><name sortKey="Miresmailli, S" uniqKey="Miresmailli S">S Miresmailli</name>
</author>
<author><name sortKey="Machial, C" uniqKey="Machial C">C Machial</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muthaiyan, A" uniqKey="Muthaiyan A">A Muthaiyan</name>
</author>
<author><name sortKey="Biswas, D" uniqKey="Biswas D">D Biswas</name>
</author>
<author><name sortKey="Crandall, Pg" uniqKey="Crandall P">PG Crandall</name>
</author>
<author><name sortKey="Wilkinson, Bj" uniqKey="Wilkinson B">BJ Wilkinson</name>
</author>
<author><name sortKey="Ricke, Sc" uniqKey="Ricke S">SC Ricke</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, CA USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26849057</article-id>
<article-id pub-id-type="pmc">4743999</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0148473</article-id>
<article-id pub-id-type="publisher-id">PONE-D-14-20130</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Organisms</subject>
<subj-group><subject>Animals</subject>
<subj-group><subject>Vertebrates</subject>
<subj-group><subject>Mammals</subject>
<subj-group><subject>Bats</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Organisms</subject>
<subj-group><subject>Fungi</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject>
<subj-group><subject>Pharmacology</subject>
<subj-group><subject>Drugs</subject>
<subj-group><subject>Antimicrobials</subject>
<subj-group><subject>Antifungals</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Microbiology</subject>
<subj-group><subject>Microbial Control</subject>
<subj-group><subject>Antimicrobials</subject>
<subj-group><subject>Antifungals</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Mycology</subject>
<subj-group><subject>Antifungals</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Biochemistry</subject>
<subj-group><subject>Lipids</subject>
<subj-group><subject>Oils</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject>
<subj-group><subject>Pharmacology</subject>
<subj-group><subject>Drugs</subject>
<subj-group><subject>Antimicrobials</subject>
<subj-group><subject>Antifungals</subject>
<subj-group><subject>Amphotericin</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Microbiology</subject>
<subj-group><subject>Microbial Control</subject>
<subj-group><subject>Antimicrobials</subject>
<subj-group><subject>Antifungals</subject>
<subj-group><subject>Amphotericin</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Mycology</subject>
<subj-group><subject>Antifungals</subject>
<subj-group><subject>Amphotericin</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Organisms</subject>
<subj-group><subject>Bacteria</subject>
<subj-group><subject>Nocardia</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Microbiology</subject>
<subj-group><subject>Medical Microbiology</subject>
<subj-group><subject>Microbial Pathogens</subject>
<subj-group><subject>Bacterial Pathogens</subject>
<subj-group><subject>Nocardia</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject>
<subj-group><subject>Pathology and Laboratory Medicine</subject>
<subj-group><subject>Pathogens</subject>
<subj-group><subject>Microbial Pathogens</subject>
<subj-group><subject>Bacterial Pathogens</subject>
<subj-group><subject>Nocardia</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Zoology</subject>
<subj-group><subject>Animal Diseases</subject>
<subj-group><subject>White Nose Syndrome</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject>
<subj-group><subject>Organisms</subject>
<subj-group><subject>Bacteria</subject>
<subj-group><subject>Actinobacteria</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Potent Inhibition of <italic>Pseudogymnoascus destructans</italic>
, the Causative Agent of White-Nose Syndrome in Bats, by Cold-Pressed, Terpeneless, Valencia Orange Oil</article-title>
<alt-title alt-title-type="running-head">Inhibition of <italic>P</italic>
. <italic>destructans</italic>
by CPT</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Boire</surname>
<given-names>Nicholas</given-names>
</name>
<xref ref-type="aff" rid="aff001"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Sean</given-names>
</name>
<xref ref-type="aff" rid="aff001"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Khuvis</surname>
<given-names>Joshua</given-names>
</name>
<xref ref-type="aff" rid="aff001"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lee</surname>
<given-names>Rick</given-names>
</name>
<xref ref-type="aff" rid="aff001"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rivers</surname>
<given-names>Jennifer</given-names>
</name>
<xref ref-type="aff" rid="aff002"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Crandall</surname>
<given-names>Philip</given-names>
</name>
<xref ref-type="aff" rid="aff003"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Keel</surname>
<given-names>M. Kevin</given-names>
</name>
<xref ref-type="aff" rid="aff004"><sup>4</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Parrish</surname>
<given-names>Nicole</given-names>
</name>
<xref ref-type="aff" rid="aff001"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor001">*</xref>
</contrib>
</contrib-group>
<aff id="aff001"><label>1</label>
<addr-line>The Johns Hopkins School of Medicine, Baltimore, MD, United States of America</addr-line>
</aff>
<aff id="aff002"><label>2</label>
<addr-line>The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States of America</addr-line>
</aff>
<aff id="aff003"><label>3</label>
<addr-line>The University of Arkansas, Fayetteville, AR, United States of America</addr-line>
</aff>
<aff id="aff004"><label>4</label>
<addr-line>UC Davis School of Veterinary Medicine, Davis, CA, United States of America</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Baker</surname>
<given-names>Michelle L.</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1"><addr-line>CSIRO, AUSTRALIA</addr-line>
</aff>
<author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>
The authors have declared that no competing interests exist.</p>
</fn>
<fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: NP SZ MKK PC. Performed the experiments: NB JK RL JR. Analyzed the data: NP NB JR PC. Contributed reagents/materials/analysis tools: NP SZ MKK. Wrote the paper: NB NP SZ MKK PC.</p>
</fn>
<corresp id="cor001">* E-mail: <email>nicholasboire@gmail.com</email>
</corresp>
</author-notes>
<pub-date pub-type="epub"><day>5</day>
<month>2</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="collection"><year>2016</year>
</pub-date>
<volume>11</volume>
<issue>2</issue>
<elocation-id>e0148473</elocation-id>
<history><date date-type="received"><day>5</day>
<month>5</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>20</day>
<month>1</month>
<year>2016</year>
</date>
</history>
<permissions><copyright-statement>© 2016 Boire et al</copyright-statement>
<copyright-year>2016</copyright-year>
<copyright-holder>Boire et al</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>
, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
</license>
</permissions>
<self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0148473.pdf"></self-uri>
<abstract><p>The causative agent of White-nose Syndrome (WNS), <italic>Pseudogymnoascus destructans</italic>
, has been shown to be fatal to several species of bats in North America. To date, no compounds or chemical control measures have been developed which eliminates the growth of the fungus in the environment or in affected animals. In the current study, we evaluated the activity of cold-pressed, terpeneless orange oil (CPT) against multiple isolates of <italic>P</italic>
. <italic>destructans in vitro</italic>
. For all assays, a modified Kirby-Bauer disk diffusion assay was used. Standardized spore suspensions were prepared, adjusted to a specific optical density, and used to plate fungal lawns. Plates were incubated at either 15°C or 4°C for up to 6 months and checked at regular intervals for growth. Once controls had grown, zones of inhibition were measured (mm) on test plates and compared to those obtained using current antifungal drugs. All <italic>P</italic>
. <italic>destructans</italic>
isolates were completely inhibited by 100% CPT (10 μL) at 1 month of incubation regardless of temperature (4°C and 15°C). Complete inhibition persisted up to 6 months following a single exposure at this concentration. Of the standard antifungals, only amphotericin B demonstrated any activity, resulting in zone diameters ranging from 58 mm to 74 mm. CPT, at the highest concentration tested (100%), had no significant effect against a variety of other environmental organisms including various filamentous fungi, bacteria and aerobic actinomycetes. Given that CPT is relatively non-toxic, the possibility exists that the all-natural, mixture could be used as an environmental pre-treatment to eradicate <italic>P</italic>
. <italic>destructans</italic>
from bat habitats. Additional studies are needed to assess any undesirable effects of CPT on bat behavior and health and overall impacts on other members of the interconnected ecosystem(s).</p>
</abstract>
<funding-group><funding-statement>The authors have no support or funding to report.</funding-statement>
</funding-group>
<counts><fig-count count="3"></fig-count>
<table-count count="1"></table-count>
<page-count count="10"></page-count>
</counts>
<custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name>
<meta-value>Our circumstances are not covered by the questions above and we need the journal's help to make the data available.</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
<notes><title>Data Availability</title>
<p>Our circumstances are not covered by the questions above and we need the journal's help to make the data available.</p>
</notes>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000372 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000372 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:4743999 |texte= Potent Inhibition of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome in Bats, by Cold-Pressed, Terpeneless, Valencia Orange Oil }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:26849057" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
![]() | This area was generated with Dilib version V0.6.25. | ![]() |