Transient Expression of Candidatus Liberibacter Asiaticus Effector Induces Cell Death in Nicotiana benthamiana
Identifieur interne : 000332 ( Pmc/Curation ); précédent : 000331; suivant : 000333Transient Expression of Candidatus Liberibacter Asiaticus Effector Induces Cell Death in Nicotiana benthamiana
Auteurs : Marco Pitino [États-Unis] ; Cheryl M. Armstrong [États-Unis] ; Liliana M. Cano [États-Unis] ; Yongping Duan [États-Unis]Source :
- Frontiers in Plant Science [ 1664-462X ] ; 2016.
Abstract
Url:
DOI: 10.3389/fpls.2016.00982
PubMed: 27458468
PubMed Central: 4933711
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transient Expression of <italic>Candidatus</italic> Liberibacter Asiaticus Effector Induces Cell Death in <italic>Nicotiana benthamiana</italic></title><author><name sortKey="Pitino, Marco" sort="Pitino, Marco" uniqKey="Pitino M" first="Marco" last="Pitino">Marco Pitino</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>U.S. Horticultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture</institution><country>Fort Pierce, FL, USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Armstrong, Cheryl M" sort="Armstrong, Cheryl M" uniqKey="Armstrong C" first="Cheryl M." last="Armstrong">Cheryl M. Armstrong</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>U.S. Horticultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture</institution><country>Fort Pierce, FL, USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Cano, Liliana M" sort="Cano, Liliana M" uniqKey="Cano L" first="Liliana M." last="Cano">Liliana M. Cano</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Institute of Food and Agricultural Sciences, Department of Plant Pathology, Indian River Research and Education Center, University of Florida</institution><country>Fort Pierce, FL, USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Duan, Yongping" sort="Duan, Yongping" uniqKey="Duan Y" first="Yongping" last="Duan">Yongping Duan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>U.S. Horticultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture</institution><country>Fort Pierce, FL, USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea></wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27458468</idno><idno type="pmc">4933711</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933711</idno><idno type="RBID">PMC:4933711</idno><idno type="doi">10.3389/fpls.2016.00982</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000333</idno><idno type="wicri:Area/Pmc/Curation">000332</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Transient Expression of <italic>Candidatus</italic> Liberibacter Asiaticus Effector Induces Cell Death in <italic>Nicotiana benthamiana</italic></title><author><name sortKey="Pitino, Marco" sort="Pitino, Marco" uniqKey="Pitino M" first="Marco" last="Pitino">Marco Pitino</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>U.S. Horticultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture</institution><country>Fort Pierce, FL, USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Armstrong, Cheryl M" sort="Armstrong, Cheryl M" uniqKey="Armstrong C" first="Cheryl M." last="Armstrong">Cheryl M. Armstrong</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>U.S. Horticultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture</institution><country>Fort Pierce, FL, USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Cano, Liliana M" sort="Cano, Liliana M" uniqKey="Cano L" first="Liliana M." last="Cano">Liliana M. Cano</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Institute of Food and Agricultural Sciences, Department of Plant Pathology, Indian River Research and Education Center, University of Florida</institution><country>Fort Pierce, FL, USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Duan, Yongping" sort="Duan, Yongping" uniqKey="Duan Y" first="Yongping" last="Duan">Yongping Duan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>U.S. Horticultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture</institution><country>Fort Pierce, FL, USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea></wicri:regionArea></affiliation></author></analytic><series><title level="j">Frontiers in Plant Science</title><idno type="eISSN">1664-462X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Candidatus</italic> Liberibacter asiaticus “Las” is a phloem-limited bacterial plant pathogen, and the most prevalent species of Liberibacter associated with citrus huanglongbing (HLB), a devastating disease of citrus worldwide. Although, the complete sequence of the Las genome provides the basis for studying functional genomics of Las and molecular mechanisms of Las-plant interactions, the functional characterization of Las effectors remains a slow process since remains to be cultured. Like other plant pathogens, Las may deliver effector proteins into host cells and modulate a variety of host cellular functions for their infection progression. In this study, we identified 16 putative Las effectors via bioinformatics, and transiently expressed them in <italic>Nicotiana benthamiana</italic>. Diverse subcellular localization with different shapes and aggregation patterns of the effector candidates were revealed by UV- microscopy after transient expression in leaf tissue. Intriguingly, one of the 16 candidates, Las5315mp (mature protein), was localized in the chloroplast and induced cell death at 3 days post inoculation (dpi) in <italic>N. benthamiana</italic>. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Plant Sci</journal-id><journal-id journal-id-type="iso-abbrev">Front Plant Sci</journal-id><journal-id journal-id-type="publisher-id">Front. Plant Sci.</journal-id><journal-title-group><journal-title>Frontiers in Plant Science</journal-title></journal-title-group><issn pub-type="epub">1664-462X</issn><publisher><publisher-name>Frontiers Media S.A.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27458468</article-id><article-id pub-id-type="pmc">4933711</article-id><article-id pub-id-type="doi">10.3389/fpls.2016.00982</article-id><article-categories><subj-group subj-group-type="heading"><subject>Plant Science</subject><subj-group><subject>Original Research</subject></subj-group></subj-group></article-categories><title-group><article-title>Transient Expression of <italic>Candidatus</italic> Liberibacter Asiaticus Effector Induces Cell Death in <italic>Nicotiana benthamiana</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Pitino</surname><given-names>Marco</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/342386/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Armstrong</surname><given-names>Cheryl M.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/350332/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Cano</surname><given-names>Liliana M.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/202283/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Duan</surname><given-names>Yongping</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn001"><sup>*</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/337521/overview"></uri></contrib></contrib-group><aff id="aff1"><sup>1</sup><institution>U.S. Horticultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture</institution><country>Fort Pierce, FL, USA</country></aff><aff id="aff2"><sup>2</sup><institution>Institute of Food and Agricultural Sciences, Department of Plant Pathology, Indian River Research and Education Center, University of Florida</institution><country>Fort Pierce, FL, USA</country></aff><author-notes><fn fn-type="edited-by"><p>Edited by: Pietro Daniele Spanu, Imperial College London, UK</p></fn><fn fn-type="edited-by"><p>Reviewed by: William Underwood, Agricultural Research Service, United States Department of Agriculture, USA; Daolong Dou, Nanjing Agricultural University, China</p></fn><corresp id="fn001">*Correspondence: Yongping Duan <email xlink:type="simple">yongping.duan@aus.usda.gov</email></corresp><fn fn-type="other" id="fn002"><p>This article was submitted to Plant Biotic Interactions, a section of the journal Frontiers in Plant Science</p></fn></author-notes><pub-date pub-type="epub"><day>06</day><month>7</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>7</volume><elocation-id>982</elocation-id><history><date date-type="received"><day>14</day><month>4</month><year>2016</year></date><date date-type="accepted"><day>21</day><month>6</month><year>2016</year></date></history><permissions><copyright-statement>Copyright © 2016 Pitino, Armstrong, Cano and Duan.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Pitino, Armstrong, Cano and Duan</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p></license></permissions><abstract><p><italic>Candidatus</italic> Liberibacter asiaticus “Las” is a phloem-limited bacterial plant pathogen, and the most prevalent species of Liberibacter associated with citrus huanglongbing (HLB), a devastating disease of citrus worldwide. Although, the complete sequence of the Las genome provides the basis for studying functional genomics of Las and molecular mechanisms of Las-plant interactions, the functional characterization of Las effectors remains a slow process since remains to be cultured. Like other plant pathogens, Las may deliver effector proteins into host cells and modulate a variety of host cellular functions for their infection progression. In this study, we identified 16 putative Las effectors via bioinformatics, and transiently expressed them in <italic>Nicotiana benthamiana</italic>. Diverse subcellular localization with different shapes and aggregation patterns of the effector candidates were revealed by UV- microscopy after transient expression in leaf tissue. Intriguingly, one of the 16 candidates, Las5315mp (mature protein), was localized in the chloroplast and induced cell death at 3 days post inoculation (dpi) in <italic>N. benthamiana</italic>. Moreover, Las5315mp induced strong callose deposition in plant cells. This study provides new insights into the localizations and potential roles of these Las effectors <italic>in planta</italic>.</p></abstract><kwd-group><kwd>huanglongbing</kwd><kwd><italic>Candidatus</italic> Liberibacter asiaticus</kwd><kwd>bacterial effectors</kwd><kwd>cell death</kwd><kwd><italic>Nicotiana benthamiana</italic></kwd><kwd>callose deposition</kwd><kwd>chloroplast localization</kwd></kwd-group><funding-group><award-group><funding-source id="cn001">Citrus Research and Development Foundation<named-content content-type="fundref-id">10.13039/100007565</named-content></funding-source><award-id rid="cn001">717</award-id></award-group></funding-group><counts><fig-count count="7"></fig-count><table-count count="1"></table-count><equation-count count="0"></equation-count><ref-count count="84"></ref-count><page-count count="13"></page-count><word-count count="8235"></word-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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