Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>Barriopsis iraniana</italic> and <italic>Phaeobotryon cupressi</italic>: two new species of the <italic>Botryosphaeriaceae</italic> from trees in Iran
</title><author><name sortKey="Abdollahzadeh, J" sort="Abdollahzadeh, J" uniqKey="Abdollahzadeh J" first="J." last="Abdollahzadeh">J. Abdollahzadeh</name><affiliation><nlm:aff id="A1">Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.</nlm:aff></affiliation></author><author><name sortKey="Mohammadi Goltapeh, E" sort="Mohammadi Goltapeh, E" uniqKey="Mohammadi Goltapeh E" first="E." last="Mohammadi Goltapeh">E. Mohammadi Goltapeh</name><affiliation><nlm:aff id="A1">Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.</nlm:aff></affiliation></author><author><name sortKey="Javadi, A" sort="Javadi, A" uniqKey="Javadi A" first="A." last="Javadi">A. Javadi</name><affiliation><nlm:aff id="A2">Department of Botany, Iranian Research Institute of Plant Protection, P.O. Box 1454, Tehran 19395, Iran.</nlm:aff></affiliation></author><author><name sortKey="Shams Bakhsh, M" sort="Shams Bakhsh, M" uniqKey="Shams Bakhsh M" first="M." last="Shams-Bakhsh">M. Shams-Bakhsh</name><affiliation><nlm:aff id="A1">Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.</nlm:aff></affiliation></author><author><name sortKey="Zare, R" sort="Zare, R" uniqKey="Zare R" first="R." last="Zare">R. Zare</name><affiliation><nlm:aff id="A2">Department of Botany, Iranian Research Institute of Plant Protection, P.O. Box 1454, Tehran 19395, Iran.</nlm:aff></affiliation></author><author><name sortKey="Phillips, A J L" sort="Phillips, A J L" uniqKey="Phillips A" first="A. J. L." last="Phillips">A. J. L. Phillips</name><affiliation><nlm:aff id="A3">Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20198156</idno><idno type="pmc">2802722</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802722</idno><idno type="RBID">PMC:2802722</idno><idno type="doi">10.3767/003158509X467552</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">001252</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>Barriopsis iraniana</italic> and <italic>Phaeobotryon cupressi</italic>: two new species of the <italic>Botryosphaeriaceae</italic> from trees in Iran
</title><author><name sortKey="Abdollahzadeh, J" sort="Abdollahzadeh, J" uniqKey="Abdollahzadeh J" first="J." last="Abdollahzadeh">J. Abdollahzadeh</name><affiliation><nlm:aff id="A1">Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.</nlm:aff></affiliation></author><author><name sortKey="Mohammadi Goltapeh, E" sort="Mohammadi Goltapeh, E" uniqKey="Mohammadi Goltapeh E" first="E." last="Mohammadi Goltapeh">E. Mohammadi Goltapeh</name><affiliation><nlm:aff id="A1">Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.</nlm:aff></affiliation></author><author><name sortKey="Javadi, A" sort="Javadi, A" uniqKey="Javadi A" first="A." last="Javadi">A. Javadi</name><affiliation><nlm:aff id="A2">Department of Botany, Iranian Research Institute of Plant Protection, P.O. Box 1454, Tehran 19395, Iran.</nlm:aff></affiliation></author><author><name sortKey="Shams Bakhsh, M" sort="Shams Bakhsh, M" uniqKey="Shams Bakhsh M" first="M." last="Shams-Bakhsh">M. Shams-Bakhsh</name><affiliation><nlm:aff id="A1">Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.</nlm:aff></affiliation></author><author><name sortKey="Zare, R" sort="Zare, R" uniqKey="Zare R" first="R." last="Zare">R. Zare</name><affiliation><nlm:aff id="A2">Department of Botany, Iranian Research Institute of Plant Protection, P.O. Box 1454, Tehran 19395, Iran.</nlm:aff></affiliation></author><author><name sortKey="Phillips, A J L" sort="Phillips, A J L" uniqKey="Phillips A" first="A. J. L." last="Phillips">A. J. L. Phillips</name><affiliation><nlm:aff id="A3">Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal;</nlm:aff></affiliation></author></analytic><series><title level="j">Persoonia</title><idno type="ISSN">0031-5850</idno><idno type="eISSN">1878-9080</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Species in the <italic>Botryosphaeriaceae</italic> are well known as pathogens and saprobes of woody hosts, but little is known about the species that occur in Iran. In a recent survey of this family in Iran two fungi with diplodia-like anamorphs were isolated from various tree hosts. These two fungi were fully characterised in terms of morphology of the anamorphs in culture, and sequences of the ITS1/ITS2 regions of the ribosomal DNA operon and partial sequences of the translation elongation factor 1-α. Phylogenetic analyses placed them within a clade consisting of <italic>Barriopsis</italic> and <italic>Phaeobotryon</italic> species, but they were clearly distinct from known species in these genera. Therefore, they are described here as two new species, namely <italic>Barriopsis iraniana</italic> on <italic>Citrus</italic>, <italic>Mangifera</italic> and <italic>Olea</italic>, and <italic>Phaeobotryon cupressi</italic> on <italic>Cupressus sempervirens</italic>.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Alves, A" uniqKey="Alves A">A Alves</name></author><author><name sortKey="Correia, A" uniqKey="Correia A">A Correia</name></author><author><name sortKey="Luque, J" uniqKey="Luque J">J Luque</name></author><author><name sortKey="Phillips, Ajl" uniqKey="Phillips A">AJL Phillips</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alves, A" uniqKey="Alves A">A Alves</name></author><author><name sortKey="Correia, A" uniqKey="Correia A">A Correia</name></author><author><name sortKey="Phillips, Ajl" uniqKey="Phillips A">AJL Phillips</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alves, A" uniqKey="Alves A">A Alves</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Correia, A" uniqKey="Correia A">A Correia</name></author><author><name sortKey="Phillips, Ajl" uniqKey="Phillips A">AJL Phillips</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arx, Ja Von" uniqKey="Arx J">JA von Arx</name></author><author><name sortKey="Muller, E" uniqKey="Muller E">E Müller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arx, Ja Von" uniqKey="Arx J">JA von Arx</name></author><author><name sortKey="Muller, E" uniqKey="Muller E">E Müller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barr, Me" uniqKey="Barr M">ME Barr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Slippers, B" uniqKey="Slippers B">B Slippers</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author><author><name sortKey="Rheeder, J" uniqKey="Rheeder J">J Rheeder</name></author><author><name sortKey="Marasas, Wfo" uniqKey="Marasas W">WFO Marasas</name></author><author><name sortKey="Phillips, Ajl" uniqKey="Phillips A">AJL Phillips</name></author><author><name sortKey="Alves, A" uniqKey="Alves A">A Alves</name></author><author><name sortKey="Burgess, T" uniqKey="Burgess T">T Burgess</name></author><author><name sortKey="Barber, P" uniqKey="Barber P">P Barber</name></author><author><name sortKey="Groenewald, Jz" uniqKey="Groenewald J">JZ Groenewald</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Damm, U" uniqKey="Damm U">U Damm</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Fourie, Ph" uniqKey="Fourie P">PH Fourie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denman, S" uniqKey="Denman S">S Denman</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Groenewald, Jz" uniqKey="Groenewald J">JZ Groenewald</name></author><author><name sortKey="Slippers, B" uniqKey="Slippers B">B Slippers</name></author><author><name sortKey="Wingfield, Bd" uniqKey="Wingfield B">BD Wingfield</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denman, S" uniqKey="Denman S">S Denman</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Taylor, Je" uniqKey="Taylor J">JE Taylor</name></author><author><name sortKey="Kang, J C" uniqKey="Kang J">J-C Kang</name></author><author><name sortKey="Pascoe, I" uniqKey="Pascoe I">I Pascoe</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Farris, Js" uniqKey="Farris J">JS Farris</name></author><author><name sortKey="Kallersjo, M" uniqKey="Kallersjo M">M Kallersjo</name></author><author><name sortKey="Kluge, Ag" uniqKey="Kluge A">AG Kluge</name></author><author><name sortKey="Bult, C" uniqKey="Bult C">C Bult</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hillis, Dm" uniqKey="Hillis D">DM Hillis</name></author><author><name sortKey="Bull, Jj" uniqKey="Bull J">JJ Bull</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huelsenbeck, Jp" uniqKey="Huelsenbeck J">JP Huelsenbeck</name></author><author><name sortKey="Bull, Jj" uniqKey="Bull J">JJ Bull</name></author><author><name sortKey="Cunningham, Cv" uniqKey="Cunningham C">CV Cunningham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jacobs, Ka" uniqKey="Jacobs K">KA Jacobs</name></author><author><name sortKey="Rehner, Sa" uniqKey="Rehner S">SA Rehner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lazzizera, C" uniqKey="Lazzizera C">C Lazzizera</name></author><author><name sortKey="Frisullo, S" uniqKey="Frisullo S">S Frisullo</name></author><author><name sortKey="Alves, A" uniqKey="Alves A">A Alves</name></author><author><name sortKey="Phillips, Ajl" uniqKey="Phillips A">AJL Phillips</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marincowitz, S" uniqKey="Marincowitz S">S Marincowitz</name></author><author><name sortKey="Groenewald, Jz" uniqKey="Groenewald J">JZ Groenewald</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Niekerk, Jm Van" uniqKey="Niekerk J">JM van Niekerk</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Groenewald, Jz" uniqKey="Groenewald J">JZ Groenewald</name></author><author><name sortKey="Fourie, Ph" uniqKey="Fourie P">PH Fourie</name></author><author><name sortKey="Halleen, F" uniqKey="Halleen F">F Halleen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="O Onnell, K" uniqKey="O Onnell K">K O’Donnell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Page, Rd" uniqKey="Page R">RD Page</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pavlic, D" uniqKey="Pavlic D">D Pavlic</name></author><author><name sortKey="Slippers, B" uniqKey="Slippers B">B Slippers</name></author><author><name sortKey="Coutinho, Ta" uniqKey="Coutinho T">TA Coutinho</name></author><author><name sortKey="Gryzenhout, M" uniqKey="Gryzenhout M">M Gryzenhout</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pavlic, D" uniqKey="Pavlic D">D Pavlic</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author><author><name sortKey="Barber, P" uniqKey="Barber P">P Barber</name></author><author><name sortKey="Slippers, B" uniqKey="Slippers B">B Slippers</name></author><author><name sortKey="Hardy, Gesj" uniqKey="Hardy G">GESJ Hardy</name></author><author><name sortKey="Burgess, Ti" uniqKey="Burgess T">TI Burgess</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petrak, F" uniqKey="Petrak F">F Petrak</name></author><author><name sortKey="Deighton, Fc" uniqKey="Deighton F">FC Deighton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Phillips, Ajl" uniqKey="Phillips A">AJL Phillips</name></author><author><name sortKey="Alves, A" uniqKey="Alves A">A Alves</name></author><author><name sortKey="Correia, A" uniqKey="Correia A">A Correia</name></author><author><name sortKey="Luque, J" uniqKey="Luque J">J Luque</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Phillips, Ajl" uniqKey="Phillips A">AJL Phillips</name></author><author><name sortKey="Alves, A" uniqKey="Alves A">A Alves</name></author><author><name sortKey="Pennycook, Sr" uniqKey="Pennycook S">SR Pennycook</name></author><author><name sortKey="Johnston, Pr" uniqKey="Johnston P">PR Johnston</name></author><author><name sortKey="Ramaley, A" uniqKey="Ramaley A">A Ramaley</name></author><author><name sortKey="Akulov, A" uniqKey="Akulov A">A Akulov</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Phillips, Ajl" uniqKey="Phillips A">AJL Phillips</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Alves, A" uniqKey="Alves A">A Alves</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rannala, B" uniqKey="Rannala B">B Rannala</name></author><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rayner, Rw" uniqKey="Rayner R">RW Rayner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reader, U" uniqKey="Reader U">U Reader</name></author><author><name sortKey="Broda, P" uniqKey="Broda P">P Broda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez, F" uniqKey="Rodriguez F">F Rodriguez</name></author><author><name sortKey="Oliver, Jf" uniqKey="Oliver J">JF Oliver</name></author><author><name sortKey="Marin, A" uniqKey="Marin A">A Marin</name></author><author><name sortKey="Medina, Jr" uniqKey="Medina J">JR Medina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ronquist, F" uniqKey="Ronquist F">F Ronquist</name></author><author><name sortKey="Huelsenbeck, Jp" uniqKey="Huelsenbeck J">JP Huelsenbeck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slippers, B" uniqKey="Slippers B">B Slippers</name></author><author><name sortKey="Fourie, G" uniqKey="Fourie G">G Fourie</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Coutinho, Ta" uniqKey="Coutinho T">TA Coutinho</name></author><author><name sortKey="Wingfield, Bd" uniqKey="Wingfield B">BD Wingfield</name></author><author><name sortKey="Carnegie, Aj" uniqKey="Carnegie A">AJ Carnegie</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slippers, B" uniqKey="Slippers B">B Slippers</name></author><author><name sortKey="Smit, Wa" uniqKey="Smit W">WA Smit</name></author><author><name sortKey="Crous, Pw" uniqKey="Crous P">PW Crous</name></author><author><name sortKey="Coutinho, Ta" uniqKey="Coutinho T">TA Coutinho</name></author><author><name sortKey="Wingfield, Bd" uniqKey="Wingfield B">BD Wingfield</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slippers, B" uniqKey="Slippers B">B Slippers</name></author><author><name sortKey="Wingfield, Mj" uniqKey="Wingfield M">MJ Wingfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stevens, Ne" uniqKey="Stevens N">NE Stevens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Swofford, Dl" uniqKey="Swofford D">DL Swofford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taylor, K" uniqKey="Taylor K">K Taylor</name></author><author><name sortKey="Barber, Pa" uniqKey="Barber P">PA Barber</name></author><author><name sortKey="Hardy, Gesj" uniqKey="Hardy G">GESJ Hardy</name></author><author><name sortKey="Burgess, Ti" uniqKey="Burgess T">TI Burgess</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Theissen, F" uniqKey="Theissen F">F Theissen</name></author><author><name sortKey="Sydow, H" uniqKey="Sydow H">H Sydow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Theissen, F" uniqKey="Theissen F">F Theissen</name></author><author><name sortKey="Sydow, H" uniqKey="Sydow H">H Sydow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, Jd" uniqKey="Thompson J">JD Thompson</name></author><author><name sortKey="Gibson, Tj" uniqKey="Gibson T">TJ Gibson</name></author><author><name sortKey="Plewniak, F" uniqKey="Plewniak F">F Plewniak</name></author><author><name sortKey="Jeanmougin, F" uniqKey="Jeanmougin F">F Jeanmougin</name></author><author><name sortKey="Higgins, Dg" uniqKey="Higgins D">DG Higgins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="White, Tj" uniqKey="White T">TJ White</name></author><author><name sortKey="Bruns, T" uniqKey="Bruns T">T Bruns</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S Lee</name></author><author><name sortKey="Taylor, J" uniqKey="Taylor J">J Taylor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, S" uniqKey="Zhou S">S Zhou</name></author><author><name sortKey="Stanosz, Gr" uniqKey="Stanosz G">GR Stanosz</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Persoonia</journal-id><journal-id journal-id-type="publisher-id">Persoonia</journal-id><journal-title-group><journal-title>Persoonia</journal-title></journal-title-group><issn pub-type="ppub">0031-5850</issn><issn pub-type="epub">1878-9080</issn><publisher><publisher-name>Nationaal Herbarium Nederland & Centraallbureau voor Schimmelcultures</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20198156</article-id><article-id pub-id-type="pmc">2802722</article-id><article-id pub-id-type="doi">10.3767/003158509X467552</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title><italic>Barriopsis iraniana</italic> and <italic>Phaeobotryon cupressi</italic>: two new species of the <italic>Botryosphaeriaceae</italic> from trees in Iran
</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Abdollahzadeh</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="A1"><sup>1</sup></xref><xref ref-type="corresp" rid="COR1"></xref></contrib><contrib contrib-type="author"><name><surname>Mohammadi Goltapeh</surname><given-names>E.</given-names></name><xref ref-type="aff" rid="A1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Javadi</surname><given-names>A.</given-names></name><xref ref-type="aff" rid="A2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Shams-bakhsh</surname><given-names>M.</given-names></name><xref ref-type="aff" rid="A1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zare</surname><given-names>R.</given-names></name><xref ref-type="aff" rid="A2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Phillips</surname><given-names>A.J.L.</given-names></name><xref ref-type="aff" rid="A3"><sup>3</sup></xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.</aff><aff id="A2"><label>2</label>Department of Botany, Iranian Research Institute of Plant Protection, P.O. Box 1454, Tehran 19395, Iran.</aff><aff id="A3"><label>3</label>Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal;</aff><author-notes><corresp id="COR1">corresponding author e-mail: <email>alp@fct.unl.pt</email>.
</corresp></author-notes><pub-date pub-type="epub"><day>16</day><month>7</month><year>2009</year></pub-date><pub-date pub-type="ppub"><month>12</month><year>2009</year></pub-date><volume>23</volume><fpage>1</fpage><lpage>8</lpage><history><date date-type="received"><day>27</day><month>4</month><year>2009</year></date><date date-type="accepted"><day>17</day><month>6</month><year>2009</year></date></history><permissions><copyright-statement>© 2009 Nationaal Herbarium Nederland & Centraalbureau voor Schimmelcultures</copyright-statement><copyright-year>2009</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode"><license-p>You are free to share - to copy, distribute and transmit the work, under the following conditions:</license-p><license-p>Attribution: You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work).</license-p><license-p>Non-commercial: You may not use this work for commercial purposes.</license-p><license-p>No derivative works: You may not alter, transform, or build upon this work.</license-p><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode">http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode.</ext-link> Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights.</license-p></license></permissions><abstract abstract-type="executive-summary"><p>Species in the <italic>Botryosphaeriaceae</italic> are well known as pathogens and saprobes of woody hosts, but little is known about the species that occur in Iran. In a recent survey of this family in Iran two fungi with diplodia-like anamorphs were isolated from various tree hosts. These two fungi were fully characterised in terms of morphology of the anamorphs in culture, and sequences of the ITS1/ITS2 regions of the ribosomal DNA operon and partial sequences of the translation elongation factor 1-α. Phylogenetic analyses placed them within a clade consisting of <italic>Barriopsis</italic> and <italic>Phaeobotryon</italic> species, but they were clearly distinct from known species in these genera. Therefore, they are described here as two new species, namely <italic>Barriopsis iraniana</italic> on <italic>Citrus</italic>, <italic>Mangifera</italic> and <italic>Olea</italic>, and <italic>Phaeobotryon cupressi</italic> on <italic>Cupressus sempervirens</italic>.</p></abstract><kwd-group><kwd><italic>Citrus</italic></kwd><kwd>EF 1-α</kwd><kwd>ITS</kwd><kwd><italic>Mangifera</italic></kwd><kwd><italic>Olea</italic></kwd><kwd>phylogeny</kwd><kwd>systematics</kwd><kwd>taxonomy</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>INTRODUCTION</title><p>Species of the <italic>Botryosphaeriaceae</italic> are cosmopolitan and occur on a wide range of plant hosts (<xref ref-type="bibr" rid="R4">von Arx & Müller 1954</xref>, <xref ref-type="bibr" rid="R6">Barr 1987</xref>). They can be primary pathogens, opportunists, endophytes or saprobes (<xref ref-type="bibr" rid="R10">Denman et al. 2000</xref>, <xref ref-type="bibr" rid="R32">Slippers & Wingfield 2007</xref>). While the family is well circumscribed, segregation of genera within the <italic>Botryosphaeriaceae</italic> has proven to be problematic. <xref ref-type="bibr" rid="R4">Von Arx & Müller (1954)</xref> made extensive synonymies under <italic>Botryosphaeria</italic> and included several genera known to have pigmented ascospores. In this way they effectively broadened the concept of <italic>Botryosphaeria</italic> to include species with hyaline ascospores, brown, aseptate ascospores, and brown, 1-septate ascospores. At least 18 anamorph genera have been associated with <italic>Botryosphaeria</italic>, including <italic>Diplodia</italic>, <italic>Lasiodiplodia</italic>, <italic>Fusicoccum</italic> and <italic>Sphaeropsis</italic>. Of these, only <italic>Fusicoccum</italic> and <italic>Diplodia</italic> were recognised by <xref ref-type="bibr" rid="R10">Denman et al. (2000)</xref>, and this was supported by several studies (<xref ref-type="bibr" rid="R14">Jacobs & Rehner 1998</xref>, <xref ref-type="bibr" rid="R41">Zhou & Stanosz 2001</xref>, <xref ref-type="bibr" rid="R1">Alves et al. 2004</xref>). <xref ref-type="bibr" rid="R20">Pavlic et al. (2004)</xref> employed morphological and phylogenetic data to separate <italic>Lasiodiplodia</italic> from <italic>Diplodia</italic>. Later, <xref ref-type="bibr" rid="R23">Phillips et al. (2005)</xref> further broadened the concept by including <italic>Dothiorella</italic> within <italic>Botryosphaeria</italic>.</p><p>In a phylogenetic study based on 28S rDNA sequence data, <xref ref-type="bibr" rid="R7">Crous et al. (2006)</xref> recognised 10 lineages within <italic>Botryosphaeria</italic> corresponding to individual genera. A further lineage representing <italic>Aplosporella</italic> was subsequently added (<xref ref-type="bibr" rid="R8">Damm et al. 2007</xref>), and <xref ref-type="bibr" rid="R24">Phillips et al. (2008)</xref> recognised a further five genera bringing the total to 16.</p><p>The <italic>Botryosphaeriaceae</italic> has been the subject of numerous critical studies on the species associated with different hosts including grapevines (<xref ref-type="bibr" rid="R17">van Niekerk et al. 2004</xref>), <italic>Eucalyptus</italic> (<xref ref-type="bibr" rid="R31">Slippers et al. 2004</xref>), <italic>Olea</italic> (<xref ref-type="bibr" rid="R15">Lazzizera et al. 2008</xref>), <italic>Prunus</italic> (<xref ref-type="bibr" rid="R32">Slippers et al. 2007</xref>, <xref ref-type="bibr" rid="R8">Damm et al. 2007</xref>) and <italic>Protea</italic> (<xref ref-type="bibr" rid="R9">Denman et al. 2003</xref>, <xref ref-type="bibr" rid="R16">Marincowitz et al. 2008</xref>). Such studies have yielded several new species, thus revealing the diversity within this family. Furthermore, intensive sampling in different regions of the world has also revealed many new species (<xref ref-type="bibr" rid="R21">Pavlic et al. 2008</xref>, <xref ref-type="bibr" rid="R36">Taylor et al. 2009</xref>). Despite the importance attributed to the species in this family, there have been no studies on the <italic>Botryosphaeriaceae</italic> in Iran.</p><p>In the course of a survey of <italic>Botryosphaeriaceae</italic> in Iran during 2005–2007, besides some 14 known species, two new species with diplodia-like conidia were encountered. The aim of the present study was to characterise the species and to describe them based on DNA sequence data and morphology.</p></sec><sec id="s2" sec-type="methods"><title>MATERIALS AND METHODS</title><sec id="s2a"><title>Isolates and isolation</title><p>Infected branches, fruits and leaves with various disease symptoms, including dieback, canker, rot and necrosis, were collected from <italic>Cupressus sempervirens</italic>, <italic>Mangifera indica</italic>, <italic>Citrus</italic> sp. and <italic>Olea</italic> sp. in northern and southern provinces of Iran. Isolations were made by transferring conidia to potato-dextrose agar (PDA; Difco Laboratories). After incubating at 25 °C for 12 h, single germinating conidia were transferred to fresh PDA plates. Some isolates were obtained by plating pieces of tissue taken from the junction of the diseased and healthy areas of the samples, after surface sterilisation (1–4 min in 70 % ethanol), on PDA supplemented with 100 mg chloramphenicol. Representative isolates were deposited at the Iranian Research Institute of Plant Protection (IRAN, Tehran, Iran) and the Centraalbureau voor Schimmelcultures (CBS, Utrecht, The Netherlands).</p></sec><sec id="s2b"><title>Morphology</title><p>Sporulation was induced by culturing the isolates on 2 % tap water agar bearing pieces of double-autoclaved, halved poplar twigs or pine needles under near-ultraviolet light in a 12 h light-dark regime for 2–6 wk at 25 °C. Vertical sections through conidiomata were made with a Leica CM1100 cryostat microtome. The conidiogenous layer was dissected from conidiomata formed in culture. Structures were mounted in 100 % lactic acid and digital images were recorded with a Leica DFC320 camera on a Leica DMR HC microscope. Measurements were made with the Leica IM500 measurement module. For each isolate the mean, standard deviation and 95 % confidence interval were calculated from measurements of at least 50 conidia. Dimensions are presented as a range with extremes in parentheses. Dimensions of other fungal structures are given as the range of at least 20 measurements. Colony morphology, colour (<xref ref-type="bibr" rid="R27">Rayner 1970</xref>), and growth rates between 5 and 35 °C in 5 °C intervals, were determined on 2 % malt extract agar (MEA; Difco Laboratories) in the dark.</p></sec><sec id="s2c"><title>DNA extraction, PCR amplification and sequencing</title><p>Isolates were grown on 2 % malt extract broth (MEB) and incubated at room temperature for 4–7 d. Mycelium was collected by filtration. Mycelial mats were washed with sterile distilled water and freeze-dried with an Edward MicroModulyo 1.5K System (England) freeze drier. Genomic DNA was obtained by a modification of the method described by <xref ref-type="bibr" rid="R28">Reader & Broda (1985)</xref>. The mycelium was ground in liquid nitrogen in 1.5 mL microtubes. Five hundred microlitres of extraction buffer (200 mM Tris-HCl pH 8.5, 250 mM NaCl, 25 mM EDTA pH 8.0, 1 % SDS) was added, the mixture thoroughly vortexed, and incubated at 65 °C for 1 h. Subsequently 500 μL of chloroform was added. The mixture was shaken gently and centrifuged at 13 000 rpm for 1 h at 4 °C. The supernatant was transferred to a new microtube and template DNA was precipitated overnight at −20 °C with 0.54 volume of ice-cold isopropanol and 3 M sodium acetate (0.1 volume). The DNA was pelleted at 13 000 rpm for 10 min at 4 °C. The resulting pellets were washed with 100 μL of cold 70 % ethanol and dried at room temperature. The dried pellets of template DNA were re-suspended in 100 μL of distilled water and incubated at 65 °C for 1 h. DNA concentrations were determined with a NanoDrop<sup>®</sup> ND-1000 spectrophotometer and DNA was stored at −80 °C.</p><p>The PCR reactions were carried out with Taq DNA polymerase, nucleotides and buffers supplied by MBI Fermentas (Vilnius, Lithuania), and PCR reaction mixtures were prepared according to <xref ref-type="bibr" rid="R1">Alves et al. (2004)</xref>, with the addition of 5 % DMSO to improve the amplification of some difficult DNA templates. All primers used were synthesised by STAB Vida Lda. (Portugal). The ITS plus D1/D2 region of the LSU and the translation elongation factor 1-α (EF-1α) were amplified using the primer pairs ITS1 (<xref ref-type="bibr" rid="R40">White et al. 1990</xref>) /NL4 (<xref ref-type="bibr" rid="R18">O’Donnell 1993</xref>) and EF1-688F/EF1-1251R, respectively, as described by <xref ref-type="bibr" rid="R3">Alves et al. (2008)</xref>. Nucleotide sequences of the ITS and EF-1α regions were determined using the primers ITS1/ITS4 (<xref ref-type="bibr" rid="R40">White et al. 1990</xref>) and EF1-688F/EF1-1251R (<xref ref-type="bibr" rid="R3">Alves et al. 2008</xref>). Both strands of the PCR products were sequenced by STAB Vida Lda (Portugal). Sequences of both DNA regions of additional isolates were retrieved from GenBank (<xref ref-type="table" rid="T1">Table 1</xref>). New sequences were deposited in GenBank (<xref ref-type="table" rid="T1">Table 1</xref>) and the alignment and trees in TreeBase (study accession number S2392, matrix accession number M4535).</p></sec><sec id="s2d"><title>Phylogenetic analyses</title><p>The nucleotide sequences were aligned with ClustalX v1.83 (<xref ref-type="bibr" rid="R39">Thompson et al. 1997</xref>), using the following parameters: pairwise alignment parameters (gap opening = 10, gap extension = 0.1) and multiple alignment parameters (gap opening = 10, gap extension = 0.2, transition weight = 0.5, delay divergent sequences = 25 %). Alignments were checked and manual adjustments were made where necessary. Phylogenetic analyses were carried out using PAUP v4.0b10 (<xref ref-type="bibr" rid="R35">Swofford 2003</xref>) for maximum-parsimony (MP) analysis and MrBayes v 3.0b4 (<xref ref-type="bibr" rid="R30">Ronquist & Huelsenbeck 2003</xref>) for the Bayesian analysis. Trees were visualised with TreeView (<xref ref-type="bibr" rid="R19">Page 1996</xref>). Maximum-parsimony analysis was performed using the heuristic search option with 1 000 random taxon additions and tree bisection and reconnection (TBR) as the branch-swapping algorithm. All characters were unordered and of equal weight and gaps were treated as fifth character. Branches of zero length were collapsed and all multiple, equally parsimonious trees were saved. The robustness of the most parsimonious trees was evaluated by 1 000 bootstrap replications (<xref ref-type="bibr" rid="R12">Hillis & Bull 1993</xref>). Other measures used were consistency index (CI), retention index (RI) and homoplasy index (HI). A partition homogeneity test was done to determine the possibility of combining the ITS and EF1-α datasets (<xref ref-type="bibr" rid="R11">Farris et al. 1995</xref>, <xref ref-type="bibr" rid="R13">Huelsenbeck et al. 1996</xref>).</p><p>Bayesian analyses employing a Markov Chain Monte Carlo (MCMC) method were performed. The general time-reversible model of evolution (<xref ref-type="bibr" rid="R29">Rodriguez et al. 1990</xref>), including estimation of invariable sites and assuming a discrete gamma distribution with six rate categories (GTR+I+Γ) was used. Four MCMC chains were run simultaneously, starting from random trees, for 10<sup>6</sup> generations. Trees were sampled every 100th generation for a total of 10<sup>4</sup> trees. The first 10<sup>3</sup> trees were discarded as the burn-in phase of each analysis. Posterior probabilities (<xref ref-type="bibr" rid="R26">Rannala & Yang 1996</xref>) were determined from a majority-rule consensus tree generated from the remaining 9 000 trees. The analysis was repeated three times starting from different random trees to ensure trees from the same tree space were being sampled during each analysis.</p></sec></sec><sec id="s3"><title>RESULTS</title><sec id="s3a"><title>DNA phylogeny</title><p>The ITS and EF1-α sequences for the 11 isolates studied were combined and aligned with 41 sequences of 22 taxa retrieved from GenBank, representing a selection of genera and species in the <italic>Botryosphaeriaceae</italic>. Incomplete portions at the ends of the sequences were excluded from the analyses. The combined dataset after alignment consisted of 941 characters including alignment gaps. A partition homogeneity test in PAUP was not significant (P = 0.52) indicating that the individual datasets were congruent and produced trees with the same topology. Therefore the two datasets were combined in a single analysis. Of the 941 characters, 446 were constant, while 10 were variable and parsimony-uninformative. Maximum parsimony analysis of the remaining 485 parsimony-informative characters resulted in a single tree of 1 340 steps (HI = 0.343). The overall topology of the 50 % majority rule consensus tree of 9 000 trees sampled in the Bayesian analysis had a similar topology as the MP tree (TreeBase S2392), which is presented in <xref ref-type="fig" rid="F1">Fig. 1</xref>.</p><p>Ten clades were identified, each corresponding to a separate genus. Isolates obtained in this study clustered in clades 1 and 2, corresponding to <italic>Phaeobotryon</italic> and <italic>Barriopsis</italic>. The five isolates from <italic>C. sempervirens</italic> clustered in a subclade of clade 1, separate from <italic>P. mamane</italic>. The remaining six isolates from this study formed a subclade within the <italic>Barriopsis</italic> clade (clade 2) separate from <italic>B. fusca</italic>. In both cases bootstrap support for the subclades was high.</p></sec><sec id="s3b"><title>Morphology</title><p>All isolates studied here produced pycnidia on pine needles and <italic>Populus</italic> twigs on WA within 2–3 wk. No ascomata were seen either on the host or in culture. Based on morphology and phylogenetic positions, these isolates were separated into two species, one in <italic>Barriopsis</italic> and the other in <italic>Phaeobotryon</italic>. On account of their unique morphology and phylogeny they are described here as two new species.</p></sec><sec id="s3c"><title>Taxonomy</title><p><bold><italic>Barriopsis iraniana</italic></bold> Abdollahzadeh, Zare & A.J.L. Phillips, <italic>sp. nov</italic>. — MycoBank MB513235; <xref ref-type="fig" rid="F2">Fig. 2</xref></p><p><italic>Teleomorph</italic>. Unknown.</p><p>Conidiomata brunnea vel nigra, uni- vel multilocularia, globosa. Cellulae conidiogenae hyalinae, cylindricae, conidio primo holoblastico, posteriora phialidica, proliferatione in eodem plano periclinaliter incrassatae. Conidia (22.7–)27–27.4(−37.7) × (12.8–)16.2–16.6(−21.5) μm, ovoidea, utrinque rotundata, primum hyalina, longitudinaliter striata, maturitate brunnea et 1–3 septa formantia.</p><p><italic>Typus</italic>. IRAN 13939F.</p><p><italic>Etymology</italic>. The name refers to Iran where the fungus was discovered.</p><p><italic>Conidiomata</italic> stromatic, pycnidial, superficial, dark brown to black, covered with dense mycelium, on pine needles mainly unilocular and up to 600 μm diam; on <italic>Populus</italic> twigs mostly multilocular, individual or aggregated, thick-walled, ostiolate. <italic>Ostiole</italic> central, circular, non-papillate. <italic>Paraphyses</italic> arising from the conidiogenous layer, extending above the level of developing conidia, up to 70 μm long, 3.5 μm wide, thin-walled, hyaline, usually aseptate, sometimes becoming up to 2–3-septate, not constricted at the septa, tip rounded, occasionally branched. <italic>Conidiophores</italic> absent. <italic>Conidiogenous</italic><italic>cells</italic> 7–12 × 3–5 μm, hyaline, thin-walled, smooth, cylindrical, holoblastic, proliferating at the same level, with visible periclinal thickening. <italic>Conidia</italic> thick-walled, initially hyaline, aseptate with longitudinal striations, striations visible on hyaline conidia even while attached to conidiogenous cells; oval, both ends broadly rounded, becoming brown, aseptate or 1–3-septate, with prominent longitudinal striations, wall smooth, (22.7–)24–30 × (12.8–)14–18(−21.5) μm, 95 % confidence limits = 27–27.4 × 16.2–16.6 μm (av. ± S.D. = 27.2 ± 1.8 × 16.4 ± 1.3 μm, l/w ratio = 1.7 ± 0.16). <italic>Chlamydospores</italic> catenate, intercalary, brown, smooth, thick-walled, formed within the agar medium.</p><p>Culture characteristics — Colonies with appressed mycelial mat and fluffy aerial mycelium in the middle, becoming dull green to olivaceous-black at the surface, and dull green to grey-olivaceous at the reverse after 2 wk in the dark at 25 °C. Colonies reaching 45–50 mm diam on MEA after 4 d in the dark at 25 °C. Cardinal temperatures for growth; min 5 °C, max > 35 °C, opt 25–30 °C.</p><p>Substrates — Endophytic in stems of <italic>Citrus</italic> sp., <italic>Mangifera indica</italic>, <italic>Olea</italic> sp.</p><p>Known distribution — Hormozgan Province, Iran.</p><p><italic>Specimens examined</italic>. <sc>Iran</sc>. Hormozgan Province, Minab, Hajikhademi, on twigs of <italic>Mangifera indica</italic>, 27 Feb. 2007, <italic>J. Abdollahzadeh & A. Javadi</italic>, holotype IRAN 13939F, culture ex-type IRAN 1448C = CBS 124698. Other isolates are listed in <xref ref-type="table" rid="T1">Table 1</xref>.</p><p>Notes — Conidia of <italic>Barriopsis iraniana</italic> are significantly larger than those of <italic>B. fusca</italic>, the only other species known in this genus. The only available culture of <italic>B. fusca</italic> (CBS 174.26, ex-type) has lost its ability to sporulate. According to <xref ref-type="bibr" rid="R34">Stevens (1926)</xref> the anamorph is lasiodiplodia-like with hyaline conidia that become dark-brown and septate with irregular longitudinal striations. These characters of the anamorphs of <italic>Barriopsis</italic> are confirmed in the present study. Furthermore, we have shown that, in contrast to <italic>Lasiodiplodia</italic>, the conidia of <italic>Barriopsis</italic> are striate at a very early stage of development and the striations are clearly visible in young, hyaline conidia (<xref ref-type="fig" rid="F1">Fig. 1d–i</xref>). This is an unusual character not found in any other genus of the <italic>Botryosphaeriaceae</italic>. We did not encounter the teleomorph of <italic>B. iraniana</italic> and it did not form in culture.</p><p><bold><italic>Phaeobotryon cupressi</italic></bold> Abdollahzadeh, Zare & A.J.L. Phillips, <italic>sp. nov</italic>. — MycoBank MB513236; <xref ref-type="fig" rid="F3">Fig. 3</xref></p><p><italic>Teleomorph</italic>. Unknown</p><p>Conidiomata brunnea vel nigra, uni- vel multilocularia, globosa. Cellulae conidiogenae hyalinae, cylindricae, holoblasticae, conidio primo holoblastico, posteriora phialidica, proliferatione in eodem plano periclinaliter incrassatae. Conidia (19.8–)24.6–25(−30) × (10.2–)12.2–12.5(−17) μm, ovoidea, utrinque rotundata, hyalina, aseptata.</p><p><italic>Typus</italic>. IRAN 13940F.</p><p><italic>Etymology</italic>. Name refers to <italic>Cupressus</italic>, the host genus on which the fungus was discovered.</p><p><italic>Conidiomata</italic> stromatic, pycnidial, superficial, dark-brown to black, mostly unilocular on pine needles and up to 650 μm diam, mostly multilocular on <italic>Populus</italic> twigs, individual or aggregated, thick-walled, ostiolate. <italic>Ostiole</italic> central, circular, non-papillate. <italic>Paraphyses</italic> hyaline, thin-walled, arising from the conidiogenous layer, extending above the level of developing conidia, up to 42 μm long, 4.8 μm wide, usually aseptate, sometimes becoming up to 2-septate, tip rounded, occasionally branched. <italic>Conidiophores</italic> absent. <italic>Conidiogenous cells</italic> hyaline, smooth, thin-walled, cylindrical, 7–14 × 2–5 μm, holoblastic, phialidic, proliferating internally with visible periclinal thickening. <italic>Conidia</italic> thick-walled, initially hyaline, oval, both ends broadly rounded, aseptate, (19.8–)21–28(−30) × (10.2–)11–15(−17) μm, 95 % confidence limits = 24.1–25 × 12.2–12.5 μm (χ̅ ± S.D. = 24.8 ± 1.9 × 12.4 ± 1.3 μm, l/w ratio = 2 ± 0.3), forming a single septum at germination, rarely becoming brown and 1-septate, internally verruculose when aged. <italic>Microconidiomata</italic> globose, dark-brown to black, superficial, occasionally immersed in pine needle or <italic>Populus</italic> tissue. <italic>Microconidiophores</italic> cylindrical, 7–13 × 1.5–2.5 μm, hyaline, aseptate becoming 1–2-septate, branched. <italic>Microconidiogenous cells</italic> hyaline, thin-walled, phialidic, proliferating internally, giving rise to periclinal thickening, 6–10 × 1–2 μm. <italic>Microconidia</italic> oval, thin-walled, hyaline, aseptate 2–4 × 1–2. <italic>Chlamydospores</italic> intercalary, brown, smooth, thick-walled, formed within the agar medium.</p><p>Cultural characteristics — Colonies with abundant aerial mycelium towards periphery, appressed in the centre, becoming grey-olivaceous to olivaceous-grey at the surface, and grey-olivaceous in reverse after 2 wk in the dark at 25 °C. Colonies on MEA reaching 46–53 mm diam after 4 d in the dark at 25 °C. Cardinal temperatures for growth; min 5 °C, max > 35 °C, opt 25 °C.</p><p>Substrate — Endophytic in stems of <italic>Cupressus sempervirens</italic>.</p><p>Known distribution — Golestan Province, Iran.</p><p><italic>Specimens examined</italic>. <sc>Iran</sc>, Golestan Province, Gorgan, City Park, on twigs of <italic>Cupressus sempervirens</italic>, 15 Aug. 2006, <italic>M.A. Aghajani</italic>, holotype IRAN 13940F, culture ex-type IRAN 1455C = CBS 124700. Other isolates are listed in <xref ref-type="table" rid="T1">Table 1</xref>.</p><p>Notes — This species differs from <italic>P. quercicola</italic> and <italic>P. mamane</italic> in its smaller conidia, and has thus far only been collected from <italic>Cupressus</italic> species. The hyaline, aseptate conidia of <italic>P. cupressi</italic> are superficially similar to those of other <italic>Diplodia</italic> species with hyaline conidia. Furthermore, conidial dimensions of <italic>P. cupressi</italic> are similar to those of <italic>Diplodia cupressi</italic> (21.5–30.5 × 12–16) as reported by <xref ref-type="bibr" rid="R2">Alves et al. (2006)</xref>. Microconidia have been reported for <italic>P. quercicola</italic> (<xref ref-type="bibr" rid="R23">Phillips et al. 2005</xref>), <italic>P. mamane</italic> (<xref ref-type="bibr" rid="R24">Phillips et al. 2008</xref>) and <italic>P. cupressi</italic> (this paper). They have also been reported in <italic>D. cupressi</italic> (<xref ref-type="bibr" rid="R2">Alves et al. 2006</xref>), but not in other <italic>Diplodia</italic> species (<xref ref-type="bibr" rid="R1">Alves et al. 2004</xref>, <xref ref-type="bibr" rid="R8">Damm et al. 2007</xref>, <xref ref-type="bibr" rid="R25">Phillips et al. 2007</xref>, <xref ref-type="bibr" rid="R15">Lazzizera et al. 2008</xref>). Thus it is possible that <italic>P. cupressi</italic> has been mistaken for <italic>D. cupressi</italic> in the past. Pycnidial paraphyses in <italic>Phaeobotryon</italic> clearly distinguish this genus from <italic>Diplodia</italic>.</p></sec></sec><sec id="s4"><title>DISCUSSION</title><p>This paper forms part of a larger study of the <italic>Botryosphaeriaceae</italic> from Iran, and is the first attempt to characterise the species present in this country. Two new species are described, one in <italic>Barriopsis</italic> and another in <italic>Phaeobotryon</italic>. These species could be distinguished based on their DNA sequence data and unique morphological characteristics. Only a few species are thus far known from these genera, and confirmed reports have been infrequent.</p><p><italic>Barriopsis</italic> was introduced when <xref ref-type="bibr" rid="R24">Phillips et al. (2008)</xref> transferred <italic>Physalospora fusca</italic> to <italic>Barriopsis fusca</italic>. <xref ref-type="bibr" rid="R34">Stevens (1926)</xref> originally placed this species in <italic>Physalospora</italic>, but was obviously hesitant to do so on account of its brown ascospores. <xref ref-type="bibr" rid="R22">Petrak & Deighton (1952)</xref> then transferred it to <italic>Phaeobotryosphaeria</italic> as <italic>Phaeobotryosphaeria fusca</italic>. Although <xref ref-type="bibr" rid="R4">von Arx & Müller (1954)</xref> considered <italic>Phaeobotryosphaeria</italic> a synonym of <italic>Botryosphaeria</italic>, <xref ref-type="bibr" rid="R24">Phillips et al. (2008)</xref> showed that it is morphologically and phylogenetically distinct from other genera in the <italic>Botryosphaeriaceae</italic>. However, the fungus considered by <xref ref-type="bibr" rid="R34">Stevens (1926)</xref> and <xref ref-type="bibr" rid="R22">Petrak & Deighton (1952)</xref> does not have apiculi on its ascospores, and thus does not fall within the concept of <italic>Phaeobotryosphaeria</italic>, which has small, hyaline apiculi on the ascospores. It was for this reason that <xref ref-type="bibr" rid="R24">Phillips et al. (2008)</xref> introduced the genus <italic>Barriopsis</italic>. <italic>Barriopsis iraniana</italic> is only the second species to be described in this genus. The new data on morphology of the anamorph, as reported in this paper, reveal further distinctions from other genera in the <italic>Botryosphaeriaceae</italic>, namely the striations visible on conidia at an early stage of development.</p><p><italic>Phaeobotryon</italic> was introduced by <xref ref-type="bibr" rid="R38">Theissen & Sydow (1915)</xref> to accommodate <italic>Dothidea cercidis</italic>. In the original description of <italic>D. cercidis</italic> the ascospores were reported to be hyaline. However, <xref ref-type="bibr" rid="R37">Theissen & Sydow (1914)</xref> observed them to become brown with age and subsequently (<xref ref-type="bibr" rid="R38">Theissen & Sydow 1915</xref>) introduced the genus <italic>Phaeobotryon</italic>. <xref ref-type="bibr" rid="R4">Von Arx & Müller (1954</xref>, <xref ref-type="bibr" rid="R5">1975</xref>) placed <italic>Phaeobotryon</italic> in synonymy with <italic>Botryosphaeria</italic> in their broader concept of this genus. However, <xref ref-type="bibr" rid="R24">Phillips et al. (2008)</xref> considered <italic>Phaeobotryon</italic> as morphologically and phylogenetically distinct from other genera in the <italic>Botryosphaeriaceae</italic> and thus reinstated the name. The genus at present consists of four species (<italic>P. cercidis</italic>, <italic>P. cupressi</italic>, <italic>P. mamane</italic> and <italic>P. quercicola</italic>), while the status of <italic>P. disruptum</italic> and <italic>P. euganeum</italic> remains uncertain. Cultures are available for only two of these, <italic>P. mamane</italic> and <italic>P. cupressi</italic>, and therefore these were the only two for which DNA sequence data are available. <xref ref-type="bibr" rid="R24">Phillips et al. (2008)</xref> did not observe conidia of <italic>P. cercidis</italic>, but they reported the conidia of <italic>P. mamane</italic> as brown and 1–2-septate. <xref ref-type="bibr" rid="R23">Phillips et al. (2005)</xref> found hyaline, aseptate conidia associated with <italic>P. quercicola</italic>, and considered these to be the anamorph. <italic>Phaeobotryon cupressi</italic> has smaller conidia than both <italic>P. mamane</italic> and <italic>P. quercicola</italic>, and the three species can be distinguished easily on the basis of conidial dimensions. No information is available regarding conidial characters of the remaining <italic>Phaeobotryon</italic> species, namely <italic>P. cercidis</italic>, <italic>P. disruptum</italic> and <italic>P. euganeum</italic>, since they were described based on features of the teleomorphs only and no anamorphic characters were considered.</p><p><italic>Barriopsis</italic> appears to be confined to regions with tropical or subtropical climates. The type species, <italic>B. fusca</italic>, was originally collected from <italic>Citrus</italic> and an unknown woody host in Cuba (<xref ref-type="bibr" rid="R34">Stevens 1926</xref>). A search of the Systematic Mycology and Microbiology Laboratory Fungal Database (April 2009; <ext-link ext-link-type="uri" xlink:href="http://nt.ars-grin.gov/fungaldatabases">http://nt.ars-grin.gov/fungaldatabases</ext-link> revealed that the majority of reports of this species are from warmer climates. In the present study, <italic>B. iraniana</italic> was found only in the subtropical southern part of Iran. In contrast to <italic>Barriopsis</italic>, <italic>Phaeobotryon</italic> appears to have a wider distribution, and species have been reported from Germany, USA (Carolina and Hawaii) and the northern, temperate regions of Iran.</p><p>Both <italic>B. iraniana</italic> and <italic>P. cupressi</italic> were isolated from diseased plants. However, pathogenicity was not tested for either species and their role as causal agents of plant diseases is not known. Furthermore, as far as we are aware, pathogenicity of none of the other species in <italic>Barriopsis</italic> and <italic>Phaeobotryon</italic> is known.</p></sec></body><back><ack><p>Much of this work was financed by the European Regional Development Fund and Fundação para a Ciência e a Tecnologia (FCT) Portugal under project PPCDT/AGR/56140/2004. A.J.L. Phillips was supported by grant number SFRH/BCC/15810/2005 from FCT, and J. Abdollahzadeh received a grant from Studienstiftung Mykologie (Köln). Dr M.A. Aghajani, Agriculture Research Centre of Gorgan, provided samples of <italic>C. sempervirens</italic> with <italic>P. cupressi</italic>. Prof. W. Gams (Baarn, The Netherlands) is thanked for useful comments on the final draft of the manuscript.</p></ack><ref-list><title>REFERENCES</title><ref id="R1"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Alves</surname><given-names>A</given-names></name><name><surname>Correia</surname><given-names>A</given-names></name><name><surname>Luque</surname><given-names>J</given-names></name><name><surname>Phillips</surname><given-names>AJL</given-names></name></person-group>
<year>2004</year>
<article-title>Botryosphaeria corticola, sp. nov. on Quercus species, with notes and description of Botryosphaeria stevensii and its anamorph, Diplodia mutila</article-title>.
<source>Mycologia</source><volume>96</volume>:
<fpage>598</fpage>–
<lpage>613</lpage></mixed-citation></ref><ref id="R2"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Alves</surname><given-names>A</given-names></name><name><surname>Correia</surname><given-names>A</given-names></name><name><surname>Phillips</surname><given-names>AJL</given-names></name></person-group>
<year>2006</year>
<article-title>Multi-gene genealogies and morphological data support Diplodia cupressi sp. nov., previously recognized as D. pinea f. sp. cupressi, as a distinct species</article-title>.
<source>Fungal Diversity</source><volume>23</volume>:
<fpage>1</fpage>–
<lpage>15</lpage></mixed-citation></ref><ref id="R3"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Alves</surname><given-names>A</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Correia</surname><given-names>A</given-names></name><name><surname>Phillips</surname><given-names>AJL</given-names></name></person-group>
<year>2008</year>
<article-title>Morphological and molecular data reveal cryptic species in Lasiodiplodia theobromae</article-title>.
<source>Fungal Diversity</source><volume>28</volume>:
<fpage>1</fpage>–
<lpage>13</lpage></mixed-citation></ref><ref id="R4"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Arx</surname><given-names>JA von</given-names></name><name><surname>Müller</surname><given-names>E</given-names></name></person-group>
<year>1954</year>
<article-title>Die Gattungen der amerosporen Pyrenomyceten</article-title>.
<source>Beiträge zur Kryptogamenflora der Schweiz 11,</source><volume>1</volume>:
<fpage>1</fpage>–
<lpage>434</lpage></mixed-citation></ref><ref id="R5"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Arx</surname><given-names>JA von</given-names></name><name><surname>Müller</surname><given-names>E</given-names></name></person-group>
<year>1975</year>
<article-title>A re-evaluation of the bitunicate ascomycetes with keys to families and genera</article-title>.
<source>Studies in Mycology</source><volume>9</volume>:
<fpage>1</fpage>–
<lpage>159</lpage></mixed-citation></ref><ref id="R6"><mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Barr</surname><given-names>ME</given-names></name></person-group>
<year>1987</year>
<source>Prodromus to class Loculoascomycetes</source>
<publisher-loc>Amherst, Massachusetts</publisher-loc>:
<publisher-name>Published by the author</publisher-name></mixed-citation></ref><ref id="R7"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Slippers</surname><given-names>B</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name><name><surname>Rheeder</surname><given-names>J</given-names></name><name><surname>Marasas</surname><given-names>WFO</given-names></name><name><surname>Phillips</surname><given-names>AJL</given-names></name><name><surname>Alves</surname><given-names>A</given-names></name><name><surname>Burgess</surname><given-names>T</given-names></name><name><surname>Barber</surname><given-names>P</given-names></name><name><surname>Groenewald</surname><given-names>JZ</given-names></name></person-group>
<year>2006</year>
<article-title>Phylogenetic lineages in the Botryosphaeriaceae</article-title>.
<source>Studies in Mycology</source><volume>55</volume>:
<fpage>235</fpage>–
<lpage>253</lpage><pub-id pub-id-type="pmid">18490983</pub-id></mixed-citation></ref><ref id="R8"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Damm</surname><given-names>U</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Fourie</surname><given-names>PH</given-names></name></person-group>
<year>2007</year>
<article-title>Botryosphaeriaceae as potential pathogens of Prunus in South Africa, with descriptions of Diplodia africana and Lasiodiplodia plurivora sp. nov</article-title>.
<source>Mycologia</source><volume>99</volume>:
<fpage>664</fpage>–
<lpage>680</lpage><pub-id pub-id-type="pmid">18268901</pub-id></mixed-citation></ref><ref id="R9"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Denman</surname><given-names>S</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Groenewald</surname><given-names>JZ</given-names></name><name><surname>Slippers</surname><given-names>B</given-names></name><name><surname>Wingfield</surname><given-names>BD</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name></person-group>
<year>2003</year>
<article-title>Circumscription of Botryosphaeria species associated with Proteaceae based on morphology and DNA sequence data</article-title>.
<source>Mycologia</source><volume>95</volume>:
<fpage>294</fpage>–
<lpage>307</lpage></mixed-citation></ref><ref id="R10"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Denman</surname><given-names>S</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Taylor</surname><given-names>JE</given-names></name><name><surname>Kang</surname><given-names>J-C</given-names></name><name><surname>Pascoe</surname><given-names>I</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name></person-group>
<year>2000</year>
<article-title>An overview of the taxonomic history of Botryosphaeria, and a re-evaluation of its anamorphs based on morphology and ITS rDNA phylogeny</article-title>.
<source>Studies in Mycology</source><volume>45</volume>:
<fpage>129</fpage>–
<lpage>140</lpage></mixed-citation></ref><ref id="R11"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Farris</surname><given-names>JS</given-names></name><name><surname>Kallersjo</surname><given-names>M</given-names></name><name><surname>Kluge</surname><given-names>AG</given-names></name><name><surname>Bult</surname><given-names>C</given-names></name></person-group>
<year>1995</year>
<article-title>Testing significance of incongruence</article-title>.
<source>Cladistics</source><volume>10</volume>:
<fpage>315</fpage>–
<lpage>319</lpage></mixed-citation></ref><ref id="R12"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hillis</surname><given-names>DM</given-names></name><name><surname>Bull</surname><given-names>JJ</given-names></name></person-group>
<year>1993</year>
<article-title>An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis</article-title>.
<source>Systematic Biology</source><volume>42</volume>:
<fpage>182</fpage>–
<lpage>192</lpage></mixed-citation></ref><ref id="R13"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Huelsenbeck</surname><given-names>JP</given-names></name><name><surname>Bull</surname><given-names>JJ</given-names></name><name><surname>Cunningham</surname><given-names>CV</given-names></name></person-group>
<year>1996</year>
<article-title>Combining data in phylogenetic analysis</article-title>.
<source>Trends in Ecology & Evolution</source><volume>11</volume>:
<fpage>152</fpage>–
<lpage>158</lpage></mixed-citation></ref><ref id="R14"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jacobs</surname><given-names>KA</given-names></name><name><surname>Rehner</surname><given-names>SA</given-names></name></person-group>
<year>1998</year>
<article-title>Comparison of cultural and morphological characters and ITS sequences in anamorphs of Botryosphaeria and related taxa</article-title>.
<source>Mycologia</source><volume>90</volume>:
<fpage>601</fpage>–
<lpage>610</lpage></mixed-citation></ref><ref id="R15"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lazzizera</surname><given-names>C</given-names></name><name><surname>Frisullo</surname><given-names>S</given-names></name><name><surname>Alves</surname><given-names>A</given-names></name><name><surname>Phillips</surname><given-names>AJL</given-names></name></person-group>
<year>2008</year>
<article-title>Morphology, phylogeny and pathogenicity of Botryosphaeria and Neofusicoccum species associated with drupe rot of olives in Southern Italy</article-title>.
<source>Plant Pathology</source><volume>57</volume>:
<fpage>948</fpage>–
<lpage>956</lpage></mixed-citation></ref><ref id="R16"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Marincowitz</surname><given-names>S</given-names></name><name><surname>Groenewald</surname><given-names>JZ</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name></person-group>
<year>2008</year>
<article-title>Species of Botryosphaeriaceae occurring on Proteaceae</article-title>.
<source>Persoonia</source><volume>21</volume>:
<fpage>111</fpage>–
<lpage>118</lpage></mixed-citation></ref><ref id="R17"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Niekerk</surname><given-names>JM van</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Groenewald</surname><given-names>JZ</given-names></name><name><surname>Fourie</surname><given-names>PH</given-names></name><name><surname>Halleen</surname><given-names>F</given-names></name></person-group>
<year>2004</year>
<article-title>DNA phylogeny, morphology and pathogenicity of Botryosphaeria species on grapevines</article-title>.
<source>Mycologia</source><volume>96</volume>:
<fpage>781</fpage>–
<lpage>798</lpage></mixed-citation></ref><ref id="R18"><mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>O’Donnell</surname><given-names>K</given-names></name></person-group>
<year>1993</year>
<article-title>Fusarium and its near relatives</article-title>.
In:
<person-group person-group-type="editor"><name><surname>Reynolds</surname><given-names>DR</given-names></name><name><surname>Taylor</surname><given-names>JW</given-names></name></person-group>(eds),
<source>The fungal holomorph: Mitotic, meiotic and pleomorphic speciation in fungal systematics: 225–233</source>
<publisher-name>CAB International</publisher-name>,
<publisher-loc>Wallingford, UK</publisher-loc></mixed-citation></ref><ref id="R19"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Page</surname><given-names>RD</given-names></name></person-group>
<year>1996</year>
<article-title>TreeView: an application to display phylogenetic trees on personal computers</article-title>.
<source>Computer Applications in the Biosciences</source><volume>12</volume>:
<fpage>357</fpage>–
<lpage>358</lpage><pub-id pub-id-type="pmid">8902363</pub-id></mixed-citation></ref><ref id="R20"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pavlic</surname><given-names>D</given-names></name><name><surname>Slippers</surname><given-names>B</given-names></name><name><surname>Coutinho</surname><given-names>TA</given-names></name><name><surname>Gryzenhout</surname><given-names>M</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name></person-group>
<year>2004</year>
<article-title>Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum in South Africa</article-title>.
<source>Studies in Mycology</source><volume>50</volume>:
<fpage>313</fpage>–
<lpage>322</lpage></mixed-citation></ref><ref id="R21"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pavlic</surname><given-names>D</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name><name><surname>Barber</surname><given-names>P</given-names></name><name><surname>Slippers</surname><given-names>B</given-names></name><name><surname>Hardy</surname><given-names>GESJ</given-names></name><name><surname>Burgess</surname><given-names>TI</given-names></name></person-group>
<year>2008</year>
<article-title>Seven new species of the Botryosphaeriaceae from baobab and other native trees in Western Australia</article-title>.
<source>Mycologia</source><volume>100</volume>:
<fpage>851</fpage>–
<lpage>866</lpage><pub-id pub-id-type="pmid">19202840</pub-id></mixed-citation></ref><ref id="R22"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Petrak</surname><given-names>F</given-names></name><name><surname>Deighton</surname><given-names>FC</given-names></name></person-group>
<year>1952</year>
<article-title>Beiträge zur Pilzeflora von Sierra Leone</article-title>.
<source>Sydowia</source><volume>6</volume>:
<fpage>309</fpage>–
<lpage>322</lpage></mixed-citation></ref><ref id="R23"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Phillips</surname><given-names>AJL</given-names></name><name><surname>Alves</surname><given-names>A</given-names></name><name><surname>Correia</surname><given-names>A</given-names></name><name><surname>Luque</surname><given-names>J</given-names></name></person-group>
<year>2005</year>
<article-title>Two new species of Botryosphaeria with brown, 1-septate ascospores and Dothiorella anamorphs</article-title>.
<source>Mycologia</source><volume>97</volume>:
<fpage>513</fpage>–
<lpage>529</lpage><pub-id pub-id-type="pmid">16396358</pub-id></mixed-citation></ref><ref id="R24"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Phillips</surname><given-names>AJL</given-names></name><name><surname>Alves</surname><given-names>A</given-names></name><name><surname>Pennycook</surname><given-names>SR</given-names></name><name><surname>Johnston</surname><given-names>PR</given-names></name><name><surname>Ramaley</surname><given-names>A</given-names></name><name><surname>Akulov</surname><given-names>A</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name></person-group>
<year>2008</year>
<article-title>Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryosphaeriaceae</article-title>.
<source>Persoonia</source><volume>21</volume>:
<fpage>29</fpage>–
<lpage>55</lpage></mixed-citation></ref><ref id="R25"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Phillips</surname><given-names>AJL</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Alves</surname><given-names>A</given-names></name></person-group>
<year>2007</year>
<article-title>Diplodia seriata, the anamorph of “Botryosphaeria” obtusa</article-title>.
<source>Fungal Diversity</source><volume>25</volume>:
<fpage>141</fpage>–
<lpage>155</lpage></mixed-citation></ref><ref id="R26"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rannala</surname><given-names>B</given-names></name><name><surname>Yang</surname><given-names>Z</given-names></name></person-group>
<year>1996</year>
<article-title>Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference</article-title>.
<source>Journal of Molecular Evolution</source><volume>43</volume>:
<fpage>304</fpage>–
<lpage>311</lpage><pub-id pub-id-type="pmid">8703097</pub-id></mixed-citation></ref><ref id="R27"><mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Rayner</surname><given-names>RW</given-names></name></person-group>
<year>1970</year>
<source>A mycological colour chart</source>
<publisher-loc>Kew, Surrey, UK</publisher-loc>:
<publisher-name>CMI and British Mycological Society</publisher-name></mixed-citation></ref><ref id="R28"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Reader</surname><given-names>U</given-names></name><name><surname>Broda</surname><given-names>P</given-names></name></person-group>
<year>1985</year>
<article-title>Rapid preparation of DNA from filamentous fungi</article-title>.
<source>Letters in Applied Microbiology</source><volume>1</volume>:
<fpage>17</fpage>–
<lpage>20</lpage></mixed-citation></ref><ref id="R29"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rodriguez</surname><given-names>F</given-names></name><name><surname>Oliver</surname><given-names>JF</given-names></name><name><surname>Marin</surname><given-names>A</given-names></name><name><surname>Medina</surname><given-names>JR</given-names></name></person-group>
<year>1990</year>
<article-title>The general stochastic model of nucleotide substitutions</article-title>.
<source>Journal of Theoretical Biology</source><volume>142</volume>:
<fpage>485</fpage>–
<lpage>501</lpage><pub-id pub-id-type="pmid">2338834</pub-id></mixed-citation></ref><ref id="R30"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ronquist</surname><given-names>F</given-names></name><name><surname>Huelsenbeck</surname><given-names>JP</given-names></name></person-group>
<year>2003</year>
<article-title>MrBayes3: Bayesian phylogenetic inference under mixed models</article-title>.
<source>Bioinformatics</source><volume>19</volume>:
<fpage>1572</fpage>–
<lpage>1574</lpage><pub-id pub-id-type="pmid">12912839</pub-id></mixed-citation></ref><ref id="R31"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Slippers</surname><given-names>B</given-names></name><name><surname>Fourie</surname><given-names>G</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Coutinho</surname><given-names>TA</given-names></name><name><surname>Wingfield</surname><given-names>BD</given-names></name><name><surname>Carnegie</surname><given-names>AJ</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name></person-group>
<year>2004</year>
<article-title>Speciation and distribution of Botryosphaeria spp. on native and introduced Eucalyptus trees in Australia and South Africa</article-title>.
<source>Studies in Mycology</source><volume>50</volume>:
<fpage>343</fpage>–
<lpage>358</lpage></mixed-citation></ref><ref id="R32"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Slippers</surname><given-names>B</given-names></name><name><surname>Smit</surname><given-names>WA</given-names></name><name><surname>Crous</surname><given-names>PW</given-names></name><name><surname>Coutinho</surname><given-names>TA</given-names></name><name><surname>Wingfield</surname><given-names>BD</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name></person-group>
<year>2007</year>
<article-title>Taxonomy, phylogeny and identification of Botryosphaeriaceae associated with pome and stone fruit trees in South Africa and other regions of the world</article-title>.
<source>Plant Pathology</source><volume>56</volume>:
<fpage>128</fpage>–
<lpage>139</lpage></mixed-citation></ref><ref id="R33"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Slippers</surname><given-names>B</given-names></name><name><surname>Wingfield</surname><given-names>MJ</given-names></name></person-group>
<year>2007</year>
<article-title>Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact</article-title>.
<source>Fungal Biology Reviews</source><volume>21</volume>:
<fpage>90</fpage>–
<lpage>106</lpage></mixed-citation></ref><ref id="R34"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Stevens</surname><given-names>NE</given-names></name></person-group>
<year>1926</year>
<article-title>Two species of Physalospora on Citrus and other hosts</article-title>.
<source>Mycologia</source><volume>18</volume>:
<fpage>206</fpage>–
<lpage>217</lpage></mixed-citation></ref><ref id="R35"><mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Swofford</surname><given-names>DL</given-names></name></person-group>
<year>2003</year>
<source>PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods) v4</source>
<publisher-name>Sinauer Associates</publisher-name>,
<publisher-loc>Sunderland, Massachusetts</publisher-loc></mixed-citation></ref><ref id="R36"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Taylor</surname><given-names>K</given-names></name><name><surname>Barber</surname><given-names>PA</given-names></name><name><surname>Hardy</surname><given-names>GESJ</given-names></name><name><surname>Burgess</surname><given-names>TI</given-names></name></person-group>
<year>2009</year>
<article-title>Botryosphaeriaceae from tuart (Eucalyptus gomphocephala) woodland, including the description of four new species</article-title>.
<source>Mycological Research</source><volume>113</volume>:
<fpage>337</fpage>–
<lpage>353</lpage><pub-id pub-id-type="pmid">19070663</pub-id></mixed-citation></ref><ref id="R37"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Theissen</surname><given-names>F</given-names></name><name><surname>Sydow</surname><given-names>H</given-names></name></person-group>
<year>1914</year>
<article-title>Dothideazeen studien II</article-title>.
<source>Annales Mycologici</source><volume>12</volume>:
<fpage>268</fpage>–
<lpage>281</lpage></mixed-citation></ref><ref id="R38"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Theissen</surname><given-names>F</given-names></name><name><surname>Sydow</surname><given-names>H</given-names></name></person-group>
<year>1915</year>
<article-title>Die Dothideales</article-title>.
<source>Annales Mycologici</source><volume>13</volume>:
<fpage>149</fpage>–
<lpage>746</lpage></mixed-citation></ref><ref id="R39"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Thompson</surname><given-names>JD</given-names></name><name><surname>Gibson</surname><given-names>TJ</given-names></name><name><surname>Plewniak</surname><given-names>F</given-names></name><name><surname>Jeanmougin</surname><given-names>F</given-names></name><name><surname>Higgins</surname><given-names>DG</given-names></name></person-group>
<year>1997</year>
<article-title>The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools</article-title>.
<source>Nucleaic Acids Research</source><volume>25</volume>:
<fpage>4876</fpage>–
<lpage>4882</lpage></mixed-citation></ref><ref id="R40"><mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>White</surname><given-names>TJ</given-names></name><name><surname>Bruns</surname><given-names>T</given-names></name><name><surname>Lee</surname><given-names>S</given-names></name><name><surname>Taylor</surname><given-names>J</given-names></name></person-group>
<year>1990</year>
<article-title>Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics</article-title>.
In:
<person-group person-group-type="editor"><name><surname>Innis</surname><given-names>MA</given-names></name><name><surname>Gelfand</surname><given-names>DH</given-names></name><name><surname>Sninsky</surname><given-names>JJ</given-names></name><name><surname>White</surname><given-names>TJ</given-names></name></person-group>(eds),
<source>PCR Protocols: a guide to methods and applications: 315–322</source>
<publisher-name>Academic Press</publisher-name>,
<publisher-loc>San Diego, California, USA</publisher-loc></mixed-citation></ref><ref id="R41"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhou</surname><given-names>S</given-names></name><name><surname>Stanosz</surname><given-names>GR</given-names></name></person-group>
<year>2001</year>
<article-title>Relationships among Botryosphaeria species and associated anamorphic fungi inferred from the analyses of ITS and 5.8S rDNA sequences</article-title>.
<source>Mycologia</source><volume>93</volume>:
<fpage>516</fpage>–
<lpage>527</lpage></mixed-citation></ref></ref-list></back><floats-group><table-wrap id="T1" orientation="portrait" position="float"><label>Table 1</label><caption><p>Isolates included in the phylogenetic study.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1">Species</th><th align="left" rowspan="1" colspan="1">Culture no.<xref ref-type="table-fn" rid="tfn1-per-23-1"><sup>1</sup></xref></th><th align="left" rowspan="1" colspan="1">Substrate</th><th align="left" rowspan="1" colspan="1">Locality</th><th align="left" rowspan="1" colspan="1">Collector</th><th colspan="2" align="left" rowspan="1">GenBank<xref ref-type="table-fn" rid="tfn2-per-23-2"><sup>2</sup></xref><hr></hr></th></tr><tr><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th align="left" rowspan="1" colspan="1">ITS</th><th align="left" rowspan="1" colspan="1">EF</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Barriopsis fusca</italic></td><td align="left" rowspan="1" colspan="1">CBS 174.26</td><td align="left" rowspan="1" colspan="1"><italic>Citrus</italic> sp</td><td align="left" rowspan="1" colspan="1">Cuba</td><td align="left" rowspan="1" colspan="1">N.E. Stevens</td><td align="left" rowspan="1" colspan="1">EU673330</td><td align="left" rowspan="1" colspan="1">EU673296</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Barriopsis iraniana</italic></td><td align="left" rowspan="1" colspan="1">IRAN1448C</td><td align="left" rowspan="1" colspan="1"><italic>Mangifera indica</italic></td><td align="left" rowspan="1" colspan="1">Minab, Iran</td><td align="left" rowspan="1" colspan="1">J. Abdollahzadeh & A. Javadi</td><td align="left" rowspan="1" colspan="1">FJ919663</td><td align="left" rowspan="1" colspan="1">FJ919652</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1449C</td><td align="left" rowspan="1" colspan="1"><italic>Olea</italic> sp.</td><td align="left" rowspan="1" colspan="1">Rodan, Iran</td><td align="left" rowspan="1" colspan="1">J. Abdollahzadeh & A. Javadi</td><td align="left" rowspan="1" colspan="1">FJ919665</td><td align="left" rowspan="1" colspan="1">FJ919654</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1450C</td><td align="left" rowspan="1" colspan="1"><italic>Citrus</italic> sp.</td><td align="left" rowspan="1" colspan="1">Minab, Iran</td><td align="left" rowspan="1" colspan="1">J. Abdollahzadeh & A. Javadi</td><td align="left" rowspan="1" colspan="1">FJ919667</td><td align="left" rowspan="1" colspan="1">FJ919656</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1451C</td><td align="left" rowspan="1" colspan="1"><italic>Citrus</italic> sp.</td><td align="left" rowspan="1" colspan="1">Minab, Iran</td><td align="left" rowspan="1" colspan="1">J. Abdollahzadeh & A. Javadi</td><td align="left" rowspan="1" colspan="1">FJ919668</td><td align="left" rowspan="1" colspan="1">FJ919657</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1452C</td><td align="left" rowspan="1" colspan="1"><italic>Citrus</italic> sp.</td><td align="left" rowspan="1" colspan="1">Minab, Iran</td><td align="left" rowspan="1" colspan="1">J. Abdollahzadeh & A. Javadi</td><td align="left" rowspan="1" colspan="1">FJ919666</td><td align="left" rowspan="1" colspan="1">FJ919655</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1453C</td><td align="left" rowspan="1" colspan="1"><italic>Mangifera indica</italic></td><td align="left" rowspan="1" colspan="1">Minab, Iran</td><td align="left" rowspan="1" colspan="1">J. Abdollahzadeh & A. Javadi</td><td align="left" rowspan="1" colspan="1">FJ919664</td><td align="left" rowspan="1" colspan="1">FJ919653</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Botryosphaeria corticis</italic></td><td align="left" rowspan="1" colspan="1">CBS119047</td><td align="left" rowspan="1" colspan="1"><italic>Vaccinium corymbosum</italic></td><td align="left" rowspan="1" colspan="1">USA</td><td align="left" rowspan="1" colspan="1">P.V. Oudemans</td><td align="left" rowspan="1" colspan="1">DQ299245</td><td align="left" rowspan="1" colspan="1">EU017539</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">ATCC22927</td><td align="left" rowspan="1" colspan="1"><italic>Vaccinium</italic> sp.</td><td align="left" rowspan="1" colspan="1">USA</td><td align="left" rowspan="1" colspan="1">R.D. Millholland</td><td align="left" rowspan="1" colspan="1">DQ299247</td><td align="left" rowspan="1" colspan="1">EU673291</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Botryosphaeria dothidea</italic></td><td align="left" rowspan="1" colspan="1">CBS 110302</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">Portugal</td><td align="left" rowspan="1" colspan="1">A.J.L. Phillips</td><td align="left" rowspan="1" colspan="1">AY259092</td><td align="left" rowspan="1" colspan="1">AY573218</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CMW 8000</td><td align="left" rowspan="1" colspan="1"><italic>Prunus</italic> sp.</td><td align="left" rowspan="1" colspan="1">Switzerland</td><td align="left" rowspan="1" colspan="1">B. Slippers</td><td align="left" rowspan="1" colspan="1">AY236949</td><td align="left" rowspan="1" colspan="1">AY236898</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Diplodia corticola</italic></td><td align="left" rowspan="1" colspan="1">CBS 112549</td><td align="left" rowspan="1" colspan="1"><italic>Quercus suber</italic></td><td align="left" rowspan="1" colspan="1">Portugal</td><td align="left" rowspan="1" colspan="1">A. Alves</td><td align="left" rowspan="1" colspan="1">AY259100</td><td align="left" rowspan="1" colspan="1">AY573227</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 112546</td><td align="left" rowspan="1" colspan="1"><italic>Quercus ilex</italic></td><td align="left" rowspan="1" colspan="1">Spain</td><td align="left" rowspan="1" colspan="1">M.E. Sánchez & A. Trapero</td><td align="left" rowspan="1" colspan="1">AY259090</td><td align="left" rowspan="1" colspan="1">EU673310</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Diplodia mutila</italic></td><td align="left" rowspan="1" colspan="1">CBS 112553</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">Portugal</td><td align="left" rowspan="1" colspan="1">A.J.L. Phillips</td><td align="left" rowspan="1" colspan="1">AY259093</td><td align="left" rowspan="1" colspan="1">AY573219</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 230.30</td><td align="left" rowspan="1" colspan="1"><italic>Phoenix dactylifera</italic></td><td align="left" rowspan="1" colspan="1">USA</td><td align="left" rowspan="1" colspan="1">L.L. Huillier</td><td align="left" rowspan="1" colspan="1">DQ458886</td><td align="left" rowspan="1" colspan="1">DQ458869</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Diplodia seriata</italic></td><td align="left" rowspan="1" colspan="1">CBS 112555</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">Portugal</td><td align="left" rowspan="1" colspan="1">A.J.L. Phillips</td><td align="left" rowspan="1" colspan="1">AY259094</td><td align="left" rowspan="1" colspan="1">AY573220</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 119049</td><td align="left" rowspan="1" colspan="1"><italic>Vitis</italic> sp.</td><td align="left" rowspan="1" colspan="1">Italy</td><td align="left" rowspan="1" colspan="1">L. Mugnai</td><td align="left" rowspan="1" colspan="1">DQ458889</td><td align="left" rowspan="1" colspan="1">DQ458874</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Dothiorella iberica</italic></td><td align="left" rowspan="1" colspan="1">CBS 115041</td><td align="left" rowspan="1" colspan="1"><italic>Quercus ilex</italic></td><td align="left" rowspan="1" colspan="1">Spain</td><td align="left" rowspan="1" colspan="1">J. Luque</td><td align="left" rowspan="1" colspan="1">AY573202</td><td align="left" rowspan="1" colspan="1">AY573222</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 113188</td><td align="left" rowspan="1" colspan="1"><italic>Quercus suber</italic></td><td align="left" rowspan="1" colspan="1">Spain</td><td align="left" rowspan="1" colspan="1">M.E. Sánchez & A. Trapero</td><td align="left" rowspan="1" colspan="1">AY573198</td><td align="left" rowspan="1" colspan="1">EU673278</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Dothiorella sarmentorum</italic></td><td align="left" rowspan="1" colspan="1">IMI 63581b</td><td align="left" rowspan="1" colspan="1"><italic>Ulmus</italic> sp.</td><td align="left" rowspan="1" colspan="1">UK</td><td align="left" rowspan="1" colspan="1">E.A. Ellis</td><td align="left" rowspan="1" colspan="1">AY573212</td><td align="left" rowspan="1" colspan="1">AY573235</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 115038</td><td align="left" rowspan="1" colspan="1"><italic>Malus pumila</italic></td><td align="left" rowspan="1" colspan="1">Netherlands</td><td align="left" rowspan="1" colspan="1">A.J.L. Phillips</td><td align="left" rowspan="1" colspan="1">AY573206</td><td align="left" rowspan="1" colspan="1">AY57322</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Lasiodiplodia gonubiensis</italic></td><td align="left" rowspan="1" colspan="1">CBS 115812</td><td align="left" rowspan="1" colspan="1"><italic>Syzygium cordatum</italic></td><td align="left" rowspan="1" colspan="1">South Africa</td><td align="left" rowspan="1" colspan="1">D. Pavlic</td><td align="left" rowspan="1" colspan="1">DQ458892</td><td align="left" rowspan="1" colspan="1">DQ458877</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Lasiodiplodia parv</italic>a</td><td align="left" rowspan="1" colspan="1">CBS 494.78</td><td align="left" rowspan="1" colspan="1"><italic>Cassava</italic>-field soil</td><td align="left" rowspan="1" colspan="1">Colombia</td><td align="left" rowspan="1" colspan="1">O. Rangel</td><td align="left" rowspan="1" colspan="1">EF622084</td><td align="left" rowspan="1" colspan="1">EF622064</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 456.78</td><td align="left" rowspan="1" colspan="1"><italic>Cassava</italic>-field soil</td><td align="left" rowspan="1" colspan="1">Colombia</td><td align="left" rowspan="1" colspan="1">O. Rangel</td><td align="left" rowspan="1" colspan="1">EF622083</td><td align="left" rowspan="1" colspan="1">EF622063</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Lasiodiplodia pseudotheobromae</italic></td><td align="left" rowspan="1" colspan="1">CBS 116459</td><td align="left" rowspan="1" colspan="1"><italic>Gmelina arborea</italic></td><td align="left" rowspan="1" colspan="1">Costa Rica</td><td align="left" rowspan="1" colspan="1">J. Carranza-Velásquez</td><td align="left" rowspan="1" colspan="1">EF622077</td><td align="left" rowspan="1" colspan="1">EF622057</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 374.54</td><td align="left" rowspan="1" colspan="1"><italic>Coffea</italic> sp.</td><td align="left" rowspan="1" colspan="1">Zaire</td><td align="left" rowspan="1" colspan="1">Unknown</td><td align="left" rowspan="1" colspan="1">EF622080</td><td align="left" rowspan="1" colspan="1">EF622059</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Neodeightonia phoenicum</italic></td><td align="left" rowspan="1" colspan="1">CBS 169.34</td><td align="left" rowspan="1" colspan="1"><italic>Phoenix dactylifera</italic></td><td align="left" rowspan="1" colspan="1">USA</td><td align="left" rowspan="1" colspan="1">H.S. Fawcett</td><td align="left" rowspan="1" colspan="1">EU673338</td><td align="left" rowspan="1" colspan="1">EU673307</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 122528</td><td align="left" rowspan="1" colspan="1"><italic>Phoenix dactylifera</italic></td><td align="left" rowspan="1" colspan="1">Spain</td><td align="left" rowspan="1" colspan="1">F. Garcia</td><td align="left" rowspan="1" colspan="1">EU673340</td><td align="left" rowspan="1" colspan="1">EU673309</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Neodeightonia subglobosa</italic></td><td align="left" rowspan="1" colspan="1">CBS 448.91</td><td align="left" rowspan="1" colspan="1">keratomycosis in eye</td><td align="left" rowspan="1" colspan="1">UK</td><td align="left" rowspan="1" colspan="1">Unknown</td><td align="left" rowspan="1" colspan="1">EU673337</td><td align="left" rowspan="1" colspan="1">EU673306</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Neofusicoccum luteum</italic></td><td align="left" rowspan="1" colspan="1">CBS 110299</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">Portugal</td><td align="left" rowspan="1" colspan="1">A.J.L. Phillips</td><td align="left" rowspan="1" colspan="1">AY259091</td><td align="left" rowspan="1" colspan="1">AY573217</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 110497</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">Portugal</td><td align="left" rowspan="1" colspan="1">A.J.L. Phillips</td><td align="left" rowspan="1" colspan="1">EU673311</td><td align="left" rowspan="1" colspan="1">EU673277</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Neofusicoccum parvum</italic></td><td align="left" rowspan="1" colspan="1">CMW 9081</td><td align="left" rowspan="1" colspan="1"><italic>Populus nigra</italic></td><td align="left" rowspan="1" colspan="1">New Zealand</td><td align="left" rowspan="1" colspan="1">G.J. Samuels</td><td align="left" rowspan="1" colspan="1">AY236943</td><td align="left" rowspan="1" colspan="1">AY236888</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 110301</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">Portugal</td><td align="left" rowspan="1" colspan="1">A.J.L. Phillips</td><td align="left" rowspan="1" colspan="1">AY259098</td><td align="left" rowspan="1" colspan="1">AY573221</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Phaeobotryon cupressi</italic></td><td align="left" rowspan="1" colspan="1">IRAN1454C</td><td align="left" rowspan="1" colspan="1"><italic>Cupressus sempervirens</italic></td><td align="left" rowspan="1" colspan="1">Gorgan, Iran</td><td align="left" rowspan="1" colspan="1">M.A. Aghajani</td><td align="left" rowspan="1" colspan="1">FJ919673</td><td align="left" rowspan="1" colspan="1">FJ919662</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1455C</td><td align="left" rowspan="1" colspan="1"><italic>Cupressus sempervirens</italic></td><td align="left" rowspan="1" colspan="1">Gorgan, Iran</td><td align="left" rowspan="1" colspan="1">M.A. Aghajani</td><td align="left" rowspan="1" colspan="1">FJ919672</td><td align="left" rowspan="1" colspan="1">FJ919661</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1456C</td><td align="left" rowspan="1" colspan="1"><italic>Cupressus sempervirens</italic></td><td align="left" rowspan="1" colspan="1">Gorgan, Iran</td><td align="left" rowspan="1" colspan="1">M.A. Aghajani</td><td align="left" rowspan="1" colspan="1">FJ919670</td><td align="left" rowspan="1" colspan="1">FJ919659</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1457C</td><td align="left" rowspan="1" colspan="1"><italic>Cupressus sempervirens</italic></td><td align="left" rowspan="1" colspan="1">Gorgan, Iran</td><td align="left" rowspan="1" colspan="1">M.A. Aghajani</td><td align="left" rowspan="1" colspan="1">FJ919669</td><td align="left" rowspan="1" colspan="1">FJ919658</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">IRAN1458C</td><td align="left" rowspan="1" colspan="1"><italic>Cupressus sempervirens</italic></td><td align="left" rowspan="1" colspan="1">Gorgan, Iran</td><td align="left" rowspan="1" colspan="1">M.A. Aghajani</td><td align="left" rowspan="1" colspan="1">FJ919671</td><td align="left" rowspan="1" colspan="1">FJ919660</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Phaeobotryon mamane</italic></td><td align="left" rowspan="1" colspan="1">CPC 12440</td><td align="left" rowspan="1" colspan="1"><italic>Sophora chrysophylla</italic></td><td align="left" rowspan="1" colspan="1">Hawaii</td><td align="left" rowspan="1" colspan="1">W. Gams</td><td align="left" rowspan="1" colspan="1">EU673332</td><td align="left" rowspan="1" colspan="1">EU673298</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CPC 12442</td><td align="left" rowspan="1" colspan="1"><italic>Sophora chrysophylla</italic></td><td align="left" rowspan="1" colspan="1">Hawaii</td><td align="left" rowspan="1" colspan="1">W. Gams</td><td align="left" rowspan="1" colspan="1">EU673333</td><td align="left" rowspan="1" colspan="1">EU673299</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Phaeobotryosphaeria citrigena</italic></td><td align="left" rowspan="1" colspan="1">ICMP 16812</td><td align="left" rowspan="1" colspan="1"><italic>Citrus sinensis</italic></td><td align="left" rowspan="1" colspan="1">New Zealand</td><td align="left" rowspan="1" colspan="1">S.R. Pennycook, P.R. Johnston & B.C. Paulus</td><td align="left" rowspan="1" colspan="1">EU673328</td><td align="left" rowspan="1" colspan="1">EU673294</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">ICMP 16818</td><td align="left" rowspan="1" colspan="1"><italic>Citrus sinensis</italic></td><td align="left" rowspan="1" colspan="1">New Zealand</td><td align="left" rowspan="1" colspan="1">S.R. Pennycook, P.R. Johnston & B.C. Paulus</td><td align="left" rowspan="1" colspan="1">EU673329</td><td align="left" rowspan="1" colspan="1">EU673295</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Phaeobotryosphaeria porosa</italic></td><td align="left" rowspan="1" colspan="1">CBS 110496</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">South Africa</td><td align="left" rowspan="1" colspan="1">J.M. van Niekerk</td><td align="left" rowspan="1" colspan="1">AY343379</td><td align="left" rowspan="1" colspan="1">AY343340</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Phaeobotryosphaeria visci</italic></td><td align="left" rowspan="1" colspan="1">CBS 100163</td><td align="left" rowspan="1" colspan="1"><italic>Viscum album</italic></td><td align="left" rowspan="1" colspan="1">Luxembourg</td><td align="left" rowspan="1" colspan="1">H.A. van der Aa</td><td align="left" rowspan="1" colspan="1">EU673324</td><td align="left" rowspan="1" colspan="1">EU673292</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 186.97</td><td align="left" rowspan="1" colspan="1"><italic>Viscum album</italic></td><td align="left" rowspan="1" colspan="1">Germany</td><td align="left" rowspan="1" colspan="1">T. Graefenhan</td><td align="left" rowspan="1" colspan="1">EU673325</td><td align="left" rowspan="1" colspan="1">EU673293</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Spencermartinsia vitícola</italic></td><td align="left" rowspan="1" colspan="1">CBS 117009</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">Spain</td><td align="left" rowspan="1" colspan="1">J. Luque & S. Martos</td><td align="left" rowspan="1" colspan="1">AY905554</td><td align="left" rowspan="1" colspan="1">AY905559</td></tr><tr><td rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">CBS 117006</td><td align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td align="left" rowspan="1" colspan="1">Spain</td><td align="left" rowspan="1" colspan="1">J. Luque & S. Martos</td><td align="left" rowspan="1" colspan="1">AY905555</td><td align="left" rowspan="1" colspan="1">AY905562</td></tr></tbody></table><table-wrap-foot><fn id="tfn1-per-23-1" fn-type="other"><p><sup>1</sup> ATCC: American Type Culture Collection; CBS: Centraalbureau voor Schimmelcultures, The Netherlands; CMW: M.J. Wingfield, FABI, University of Pretoria, South Africa; CPC: Collection of Pedro Crous housed at CBS; ICMP: International Collection of Micro-organisms from Plants, Landcare Research, New Zealand; IMI: CABI Bioscience, Egham, UK; IRAN: Iranian Fungal Culture Collection, Iranian Research Institute of Plant Protection, Iran.</p></fn><fn id="tfn2-per-23-2" fn-type="other"><p><sup>2</sup> ITS: Internal transcribed spacers 1 and 2 together with 5.8S nrDNA; EF: Translation elongation factor 1-α partial sequence.</p></fn></table-wrap-foot></table-wrap><fig id="F1" orientation="portrait" position="float"><label>Fig. 1</label><caption><p>Single most parsimonious tree resulting from maximum parsimony analysis of combined ITS and EF1-α sequence data. MP bootstrap values are given at the nodes. The tree was rooted to two isolates of <italic>Pseudofusicoccum stromaticum</italic>. The new species are in <bold>bold</bold> face.</p></caption><graphic xlink:href="per-23-1-g001"></graphic></fig><fig id="F2" orientation="portrait" position="float"><label>Fig. 2</label><caption><p><italic>Barriopsis iraniana</italic> holotype. a. Conidiomata on pine needles in culture; b, c. conidia developing on conidiogenous cells between paraphyses; d. young conidium showing longitudinal striations while attached to a conidiogenous cell; e. hyaline, striate conidia; f–i. hyaline and brown, striate conidia, 1- and 3-septate conidia can be seen in f and g; j. catenulate chlamydospores. — Scale bars: a = 250 μm; b, c, e–i = 10 μm; d= 5 μm; j = 40 μm.</p></caption><graphic xlink:href="per-23-1-g002"></graphic></fig><fig id="F3" orientation="portrait" position="float"><label>Fig. 3</label><caption><p><italic>Phaeobotryon cupressi</italic> holotype. a. Conidiomata formed on pine needles in culture; b. sectioned conidioma; c. paraphyses and developing conidia; d, e. microconidiogenous cells; f. microconidia; g. conidia and conidiogenous cells; h. hyaline conidia; i, j. brown, aseptate conidia; k. germinating conidia; l. chlamydospores and a hyaline conidium. — Scale bars: a = 250 μm; b = 100 μm; c, h, i, k, l = 10 μm; d, e = 2.5 μm; f, g, j = 5 μm.</p></caption><graphic xlink:href="per-23-1-g003"></graphic></fig></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001252 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 001252 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.25. |  |