Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A fruit quality gene map of <italic>Prunus</italic></title><author><name sortKey="Ogundiwin, Ebenezer A" sort="Ogundiwin, Ebenezer A" uniqKey="Ogundiwin E" first="Ebenezer A" last="Ogundiwin">Ebenezer A. Ogundiwin</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author><author><name sortKey="Peace, Cameron P" sort="Peace, Cameron P" uniqKey="Peace C" first="Cameron P" last="Peace">Cameron P. Peace</name><affiliation><nlm:aff id="I2">Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA 99164 USA</nlm:aff></affiliation></author><author><name sortKey="Gradziel, Thomas M" sort="Gradziel, Thomas M" uniqKey="Gradziel T" first="Thomas M" last="Gradziel">Thomas M. Gradziel</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author><author><name sortKey="Parfitt, Dan E" sort="Parfitt, Dan E" uniqKey="Parfitt D" first="Dan E" last="Parfitt">Dan E. Parfitt</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author><author><name sortKey="Bliss, Fredrick A" sort="Bliss, Fredrick A" uniqKey="Bliss F" first="Fredrick A" last="Bliss">Fredrick A. Bliss</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author><author><name sortKey="Crisosto, Carlos H" sort="Crisosto, Carlos H" uniqKey="Crisosto C" first="Carlos H" last="Crisosto">Carlos H. Crisosto</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19995417</idno><idno type="pmc">2797820</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797820</idno><idno type="RBID">PMC:2797820</idno><idno type="doi">10.1186/1471-2164-10-587</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000E09</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A fruit quality gene map of <italic>Prunus</italic></title><author><name sortKey="Ogundiwin, Ebenezer A" sort="Ogundiwin, Ebenezer A" uniqKey="Ogundiwin E" first="Ebenezer A" last="Ogundiwin">Ebenezer A. Ogundiwin</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author><author><name sortKey="Peace, Cameron P" sort="Peace, Cameron P" uniqKey="Peace C" first="Cameron P" last="Peace">Cameron P. Peace</name><affiliation><nlm:aff id="I2">Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA 99164 USA</nlm:aff></affiliation></author><author><name sortKey="Gradziel, Thomas M" sort="Gradziel, Thomas M" uniqKey="Gradziel T" first="Thomas M" last="Gradziel">Thomas M. Gradziel</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author><author><name sortKey="Parfitt, Dan E" sort="Parfitt, Dan E" uniqKey="Parfitt D" first="Dan E" last="Parfitt">Dan E. Parfitt</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author><author><name sortKey="Bliss, Fredrick A" sort="Bliss, Fredrick A" uniqKey="Bliss F" first="Fredrick A" last="Bliss">Fredrick A. Bliss</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author><author><name sortKey="Crisosto, Carlos H" sort="Crisosto, Carlos H" uniqKey="Crisosto C" first="Carlos H" last="Crisosto">Carlos H. Crisosto</name><affiliation><nlm:aff id="I1">Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</nlm:aff></affiliation></author></analytic><series><title level="j">BMC Genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p><italic>Prunus </italic>fruit development, growth, ripening, and senescence includes major biochemical and sensory changes in texture, color, and flavor. The genetic dissection of these complex processes has important applications in crop improvement, to facilitate maximizing and maintaining stone fruit quality from production and processing through to marketing and consumption. Here we present an integrated fruit quality gene map of <italic>Prunus </italic>containing 133 genes putatively involved in the determination of fruit texture, pigmentation, flavor, and chilling injury resistance.</p></sec><sec><title>Results</title><p>A genetic linkage map of 211 markers was constructed for an intraspecific peach (<italic>Prunus persica</italic>) progeny population, Pop-DG, derived from a canning peach cultivar 'Dr. Davis' and a fresh market cultivar 'Georgia Belle'. The Pop-DG map covered 818 cM of the peach genome and included three morphological markers, 11 ripening candidate genes, 13 cold-responsive genes, 21 novel EST-SSRs from the ChillPeach database, 58 previously reported SSRs, 40 RAFs, 23 SRAPs, 14 IMAs, and 28 accessory markers from candidate gene amplification. The Pop-DG map was co-linear with the <italic>Prunus </italic>reference T × E map, with 39 SSR markers in common to align the maps. A further 158 markers were bin-mapped to the reference map: 59 ripening candidate genes, 50 cold-responsive genes, and 50 novel EST-SSRs from ChillPeach, with deduced locations in Pop-DG via comparative mapping. Several candidate genes and EST-SSRs co-located with previously reported major trait loci and quantitative trait loci for chilling injury symptoms in Pop-DG.</p></sec><sec><title>Conclusion</title><p>The candidate gene approach combined with bin-mapping and availability of a community-recognized reference genetic map provides an efficient means of locating genes of interest in a target genome. We highlight the co-localization of fruit quality candidate genes with previously reported fruit quality QTLs. The fruit quality gene map developed here is a valuable tool for dissecting the genetic architecture of fruit quality traits in <italic>Prunus </italic>crops.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Abbott, A" uniqKey="Abbott A">A Abbott</name></author><author><name sortKey="Georgi, L" uniqKey="Georgi L">L Georgi</name></author><author><name sortKey="Yvergniaux, D" uniqKey="Yvergniaux D">D Yvergniaux</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Blenda, A" uniqKey="Blenda A">A Blenda</name></author><author><name sortKey="Reighard, G" uniqKey="Reighard G">G Reighard</name></author><author><name sortKey="Inigo, M" uniqKey="Inigo M">M Inigo</name></author><author><name sortKey="Sosinski, B" uniqKey="Sosinski B">B Sosinski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shulaev, V" uniqKey="Shulaev V">V Shulaev</name></author><author><name sortKey="Korban, Ss" uniqKey="Korban S">SS Korban</name></author><author><name sortKey="Sosinski, B" uniqKey="Sosinski B">B Sosinski</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author><author><name sortKey="Aldwinckle, Hs" uniqKey="Aldwinckle H">HS Aldwinckle</name></author><author><name sortKey="Folta, Km" uniqKey="Folta K">KM Folta</name></author><author><name sortKey="Iezzoni, A" uniqKey="Iezzoni A">A Iezzoni</name></author><author><name sortKey="Main, D" uniqKey="Main D">D Main</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Dandekar, Am" uniqKey="Dandekar A">AM Dandekar</name></author><author><name sortKey="Lewers, K" uniqKey="Lewers K">K Lewers</name></author><author><name sortKey="Brown, Sk" uniqKey="Brown S">SK Brown</name></author><author><name sortKey="Davis, Tm" uniqKey="Davis T">TM Davis</name></author><author><name sortKey="Gardiner, Se" uniqKey="Gardiner S">SE Gardiner</name></author><author><name sortKey="Potter, D" uniqKey="Potter D">D Potter</name></author><author><name sortKey="Veilleux, Re" uniqKey="Veilleux R">RE Veilleux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Byrne, Dh" uniqKey="Byrne D">DH Byrne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhebentyayeva, Tn" uniqKey="Zhebentyayeva T">TN Zhebentyayeva</name></author><author><name sortKey="Swire Clark, G" uniqKey="Swire Clark G">G Swire-Clark</name></author><author><name sortKey="Georgi, Ll" uniqKey="Georgi L">LL Georgi</name></author><author><name sortKey="Garay, L" uniqKey="Garay L">L Garay</name></author><author><name sortKey="Jung, S" uniqKey="Jung S">S Jung</name></author><author><name sortKey="Forrest, S" uniqKey="Forrest S">S Forrest</name></author><author><name sortKey="Blenda, Av" uniqKey="Blenda A">AV Blenda</name></author><author><name sortKey="Blackmon, B" uniqKey="Blackmon B">B Blackmon</name></author><author><name sortKey="Mook, J" uniqKey="Mook J">J Mook</name></author><author><name sortKey="Horn, R" uniqKey="Horn R">R Horn</name></author><author><name sortKey="Howad, W" uniqKey="Howad W">W Howad</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Main, D" uniqKey="Main D">D Main</name></author><author><name sortKey="Tomkins, Jp" uniqKey="Tomkins J">JP Tomkins</name></author><author><name sortKey="Sosinski, B" uniqKey="Sosinski B">B Sosinski</name></author><author><name sortKey="Baird, Wv" uniqKey="Baird W">WV Baird</name></author><author><name sortKey="Reighard, Gl" uniqKey="Reighard G">GL Reighard</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jung, S" uniqKey="Jung S">S Jung</name></author><author><name sortKey="Staton, M" uniqKey="Staton M">M Staton</name></author><author><name sortKey="Lee, T" uniqKey="Lee T">T Lee</name></author><author><name sortKey="Blenda, A" uniqKey="Blenda A">A Blenda</name></author><author><name sortKey="Svancara, R" uniqKey="Svancara R">R Svancara</name></author><author><name sortKey="Abbott, A" uniqKey="Abbott A">A Abbott</name></author><author><name sortKey="Main, D" uniqKey="Main D">D Main</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Joobeur, T" uniqKey="Joobeur T">T Joobeur</name></author><author><name sortKey="Viruel, Ma" uniqKey="Viruel M">MA Viruel</name></author><author><name sortKey="De Vicente, Mc" uniqKey="De Vicente M">MC de Vicente</name></author><author><name sortKey="Jauregui, B" uniqKey="Jauregui B">B Jauregui</name></author><author><name sortKey="Ballester, J" uniqKey="Ballester J">J Ballester</name></author><author><name sortKey="Dettori, Mt" uniqKey="Dettori M">MT Dettori</name></author><author><name sortKey="Verde, I" uniqKey="Verde I">I Verde</name></author><author><name sortKey="Truco, Mj" uniqKey="Truco M">MJ Truco</name></author><author><name sortKey="Messeguer, R" uniqKey="Messeguer R">R Messeguer</name></author><author><name sortKey="Battle, I" uniqKey="Battle I">I Battle</name></author><author><name sortKey="Quarta, R" uniqKey="Quarta R">R Quarta</name></author><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aranzana, Mj" uniqKey="Aranzana M">MJ Aranzana</name></author><author><name sortKey="Pineda, A" uniqKey="Pineda A">A Pineda</name></author><author><name sortKey="Cosson, P" uniqKey="Cosson P">P Cosson</name></author><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Ascasibar, J" uniqKey="Ascasibar J">J Ascasibar</name></author><author><name sortKey="Cipriani, G" uniqKey="Cipriani G">G Cipriani</name></author><author><name sortKey="Ryder, Cd" uniqKey="Ryder C">CD Ryder</name></author><author><name sortKey="Testolini, R" uniqKey="Testolini R">R Testolini</name></author><author><name sortKey="Abbott, A" uniqKey="Abbott A">A Abbott</name></author><author><name sortKey="King, Gj" uniqKey="King G">GJ King</name></author><author><name sortKey="Iezzoni, Af" uniqKey="Iezzoni A">AF Iezzoni</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Graziano, E" uniqKey="Graziano E">E Graziano</name></author><author><name sortKey="Joobeur, T" uniqKey="Joobeur T">T Joobeur</name></author><author><name sortKey="Garriga Caldere, F" uniqKey="Garriga Caldere F">F Garriga-Caldere</name></author><author><name sortKey="Cosson, P" uniqKey="Cosson P">P Cosson</name></author><author><name sortKey="Howard, W" uniqKey="Howard W">W Howard</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Yamamoto, T" uniqKey="Yamamoto T">T Yamamoto</name></author><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Scorza, R" uniqKey="Scorza R">R Scorza</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Howad, W" uniqKey="Howad W">W Howad</name></author><author><name sortKey="Yamamoto, T" uniqKey="Yamamoto T">T Yamamoto</name></author><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Testolin, R" uniqKey="Testolin R">R Testolin</name></author><author><name sortKey="Cosson, P" uniqKey="Cosson P">P Cosson</name></author><author><name sortKey="Cipriani, G" uniqKey="Cipriani G">G Cipriani</name></author><author><name sortKey="Monforte, Aj" uniqKey="Monforte A">AJ Monforte</name></author><author><name sortKey="Georgi, L" uniqKey="Georgi L">L Georgi</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Foolad, Mr" uniqKey="Foolad M">MR Foolad</name></author><author><name sortKey="Arulsekar, S" uniqKey="Arulsekar S">S Arulsekar</name></author><author><name sortKey="Becerra, V" uniqKey="Becerra V">V Becerra</name></author><author><name sortKey="Bliss, Fa" uniqKey="Bliss F">FA Bliss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bliss, Fa" uniqKey="Bliss F">FA Bliss</name></author><author><name sortKey="Arulsekar, S" uniqKey="Arulsekar S">S Arulsekar</name></author><author><name sortKey="Foolad, Mr" uniqKey="Foolad M">MR Foolad</name></author><author><name sortKey="Becerra, V" uniqKey="Becerra V">V Becerra</name></author><author><name sortKey="Gillen, Am" uniqKey="Gillen A">AM Gillen</name></author><author><name sortKey="Warburton, Ml" uniqKey="Warburton M">ML Warburton</name></author><author><name sortKey="Dandekar, Am" uniqKey="Dandekar A">AM Dandekar</name></author><author><name sortKey="Kocsisne, Gm" uniqKey="Kocsisne G">GM Kocsisne</name></author><author><name sortKey="Mydin, Kk" uniqKey="Mydin K">KK Mydin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Cosson, P" uniqKey="Cosson P">P Cosson</name></author><author><name sortKey="Howad, W" uniqKey="Howad W">W Howad</name></author><author><name sortKey="Capdeville, G" uniqKey="Capdeville G">G Capdeville</name></author><author><name sortKey="Bosselut, N" uniqKey="Bosselut N">N Bosselut</name></author><author><name sortKey="Claverie, M" uniqKey="Claverie M">M Claverie</name></author><author><name sortKey="Voisin, R" uniqKey="Voisin R">R Voisin</name></author><author><name sortKey="Poizat, C" uniqKey="Poizat C">C Poizat</name></author><author><name sortKey="Lafargue, B" uniqKey="Lafargue B">B Lafargue</name></author><author><name sortKey="Baron, O" uniqKey="Baron O">O Baron</name></author><author><name sortKey="Laigret, F" uniqKey="Laigret F">F Laigret</name></author><author><name sortKey="Kleinhentz, M" uniqKey="Kleinhentz M">M Kleinhentz</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Esmenjaud, D" uniqKey="Esmenjaud D">D Esmenjaud</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rajapakse, S" uniqKey="Rajapakse S">S Rajapakse</name></author><author><name sortKey="Belthoff, Le" uniqKey="Belthoff L">LE Belthoff</name></author><author><name sortKey="He, G" uniqKey="He G">G He</name></author><author><name sortKey="Estager, Ae" uniqKey="Estager A">AE Estager</name></author><author><name sortKey="Scorza, R" uniqKey="Scorza R">R Scorza</name></author><author><name sortKey="Verde, I" uniqKey="Verde I">I Verde</name></author><author><name sortKey="Ballard, Re" uniqKey="Ballard R">RE Ballard</name></author><author><name sortKey="Baird, Wv" uniqKey="Baird W">WV Baird</name></author><author><name sortKey="Callahan, A" uniqKey="Callahan A">A Callahan</name></author><author><name sortKey="Monet, R" uniqKey="Monet R">R Monet</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Viruel, Ma" uniqKey="Viruel M">MA Viruel</name></author><author><name sortKey="Messeguer, R" uniqKey="Messeguer R">R Messeguer</name></author><author><name sortKey="De Vicente, Mc" uniqKey="De Vicente M">MC de Vicente</name></author><author><name sortKey="Garcia Mas, J" uniqKey="Garcia Mas J">J Garcia-Mas</name></author><author><name sortKey="Puigdomenech, P" uniqKey="Puigdomenech P">P Puigdomenech</name></author><author><name sortKey="Vargas, Fj" uniqKey="Vargas F">FJ Vargas</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Joobeur, T" uniqKey="Joobeur T">T Joobeur</name></author><author><name sortKey="Periam, N" uniqKey="Periam N">N Periam</name></author><author><name sortKey="De Vicente, Mc" uniqKey="De Vicente M">MC de Vicente</name></author><author><name sortKey="King, Gj" uniqKey="King G">GJ King</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanchez Perez, R" uniqKey="Sanchez Perez R">R Sánchez-Pérez</name></author><author><name sortKey="Howad, W" uniqKey="Howad W">W Howad</name></author><author><name sortKey="Dicenta, F" uniqKey="Dicenta F">F Dicenta</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Martinez G Mez, P" uniqKey="Martinez G Mez P">P Martínez-Gómez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hurtado, Ma" uniqKey="Hurtado M">MA Hurtado</name></author><author><name sortKey="Vilanova, S" uniqKey="Vilanova S">S Vilanova</name></author><author><name sortKey="Romero, C" uniqKey="Romero C">C Romero</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author><author><name sortKey="Llacer, G" uniqKey="Llacer G">G Llacer</name></author><author><name sortKey="Badenes, Ml" uniqKey="Badenes M">ML Badenes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vilanova, S" uniqKey="Vilanova S">S Vilanova</name></author><author><name sortKey="Romero, C" uniqKey="Romero C">C Romero</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author><author><name sortKey="Llacer, G" uniqKey="Llacer G">G Llacer</name></author><author><name sortKey="Badenes, Ml" uniqKey="Badenes M">ML Badenes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lambert, P" uniqKey="Lambert P">P Lambert</name></author><author><name sortKey="Hagen, Ls" uniqKey="Hagen L">LS Hagen</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Audergon, Jm" uniqKey="Audergon J">JM Audergon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dondini, L" uniqKey="Dondini L">L Dondini</name></author><author><name sortKey="Lain, O" uniqKey="Lain O">O Lain</name></author><author><name sortKey="Geuna, F" uniqKey="Geuna F">F Geuna</name></author><author><name sortKey="Banfi, R" uniqKey="Banfi R">R Banfi</name></author><author><name sortKey="Gaiotti, F" uniqKey="Gaiotti F">F Gaiotti</name></author><author><name sortKey="Tartarini, S" uniqKey="Tartarini S">S Tartarini</name></author><author><name sortKey="Bassi, D" uniqKey="Bassi D">D Bassi</name></author><author><name sortKey="Testolin, R" uniqKey="Testolin R">R Testolin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olmstead, Jw" uniqKey="Olmstead J">JW Olmstead</name></author><author><name sortKey="Sebolt, Am" uniqKey="Sebolt A">AM Sebolt</name></author><author><name sortKey="Cabrera, A" uniqKey="Cabrera A">A Cabrera</name></author><author><name sortKey="Sooriyapathirana, Sh" uniqKey="Sooriyapathirana S">SH Sooriyapathirana</name></author><author><name sortKey="Iriarte, G" uniqKey="Iriarte G">G Iriarte</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Chen, Cy" uniqKey="Chen C">CY Chen</name></author><author><name sortKey="Knaap, E Van Der" uniqKey="Knaap E">E van der Knaap</name></author><author><name sortKey="Iezzoni, Af" uniqKey="Iezzoni A">AF Iezzoni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chaparro, Jx" uniqKey="Chaparro J">JX Chaparro</name></author><author><name sortKey="Werner, Dj" uniqKey="Werner D">DJ Werner</name></author><author><name sortKey="O Malley, D" uniqKey="O Malley D">D O'Malley</name></author><author><name sortKey="Sederoff, Rr" uniqKey="Sederoff R">RR Sederoff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Pronier, V" uniqKey="Pronier V">V Pronier</name></author><author><name sortKey="Parvery, C" uniqKey="Parvery C">C Parvery</name></author><author><name sortKey="Rothan, C" uniqKey="Rothan C">C Rothan</name></author><author><name sortKey="Guye, A" uniqKey="Guye A">A Guye</name></author><author><name sortKey="Monet, R" uniqKey="Monet R">R Monet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author><author><name sortKey="Rajapakse, S" uniqKey="Rajapakse S">S Rajapakse</name></author><author><name sortKey="Sosinski, B" uniqKey="Sosinski B">B Sosinski</name></author><author><name sortKey="Lu, Zx" uniqKey="Lu Z">ZX Lu</name></author><author><name sortKey="Sossey Alaoui, K" uniqKey="Sossey Alaoui K">K Sossey-Alaoui</name></author><author><name sortKey="Gannavarapu, M" uniqKey="Gannavarapu M">M Gannavarapu</name></author><author><name sortKey="Reighard, G" uniqKey="Reighard G">G Reighard</name></author><author><name sortKey="Ballard, Re" uniqKey="Ballard R">RE Ballard</name></author><author><name sortKey="Baird, Wv" uniqKey="Baird W">WV Baird</name></author><author><name sortKey="Scorza, R" uniqKey="Scorza R">R Scorza</name></author><author><name sortKey="Callahan, A" uniqKey="Callahan A">A Callahan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, Z X" uniqKey="Lu Z">Z-X Lu</name></author><author><name sortKey="Sosinski, B" uniqKey="Sosinski B">B Sosinski</name></author><author><name sortKey="Reighard, Gl" uniqKey="Reighard G">GL Reighard</name></author><author><name sortKey="Baird, Wv" uniqKey="Baird W">WV Baird</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamamoto, T" uniqKey="Yamamoto T">T Yamamoto</name></author><author><name sortKey="Shimada, T" uniqKey="Shimada T">T Shimada</name></author><author><name sortKey="Imai, T" uniqKey="Imai T">T Imai</name></author><author><name sortKey="Yaegaki, H" uniqKey="Yaegaki H">H Yaegaki</name></author><author><name sortKey="Haji, T" uniqKey="Haji T">T Haji</name></author><author><name sortKey="Matsuta, N" uniqKey="Matsuta N">N Matsuta</name></author><author><name sortKey="Yamaguchi, M" uniqKey="Yamaguchi M">M Yamaguchi</name></author><author><name sortKey="Hayashi, T" uniqKey="Hayashi T">T Hayashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Cosson, P" uniqKey="Cosson P">P Cosson</name></author><author><name sortKey="Boudehri, K" uniqKey="Boudehri K">K Boudehri</name></author><author><name sortKey="Renaud, C" uniqKey="Renaud C">C Renaud</name></author><author><name sortKey="Capdeville, G" uniqKey="Capdeville G">G Capdeville</name></author><author><name sortKey="Tauzin, Y" uniqKey="Tauzin Y">Y Tauzin</name></author><author><name sortKey="Laigret, F" uniqKey="Laigret F">F Laigret</name></author><author><name sortKey="Moing, A" uniqKey="Moing A">A Moing</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bailey, Js" uniqKey="Bailey J">JS Bailey</name></author><author><name sortKey="French, Ap" uniqKey="French A">AP French</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peace, Cp" uniqKey="Peace C">CP Peace</name></author><author><name sortKey="Ahmad, R" uniqKey="Ahmad R">R Ahmad</name></author><author><name sortKey="Gradziel, Tm" uniqKey="Gradziel T">TM Gradziel</name></author><author><name sortKey="Dandekar, Am" uniqKey="Dandekar A">AM Dandekar</name></author><author><name sortKey="Crisosto, Ch" uniqKey="Crisosto C">CH Crisosto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peace, Cp" uniqKey="Peace C">CP Peace</name></author><author><name sortKey="Crisosto, Ch" uniqKey="Crisosto C">CH Crisosto</name></author><author><name sortKey="Gradziel, Tm" uniqKey="Gradziel T">TM Gradziel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogundiwin, Ea" uniqKey="Ogundiwin E">EA Ogundiwin</name></author><author><name sortKey="Peace, Cp" uniqKey="Peace C">CP Peace</name></author><author><name sortKey="Nicolet, Cm" uniqKey="Nicolet C">CM Nicolet</name></author><author><name sortKey="Rashbrook, Vk" uniqKey="Rashbrook V">VK Rashbrook</name></author><author><name sortKey="Gradziel, Tm" uniqKey="Gradziel T">TM Gradziel</name></author><author><name sortKey="Bliss, Fa" uniqKey="Bliss F">FA Bliss</name></author><author><name sortKey="Parfitt, D" uniqKey="Parfitt D">D Parfitt</name></author><author><name sortKey="Crisosto, Ch" uniqKey="Crisosto C">CH Crisosto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quilot, B" uniqKey="Quilot B">B Quilot</name></author><author><name sortKey="Wu, Bh" uniqKey="Wu B">BH Wu</name></author><author><name sortKey="Kervella, J" uniqKey="Kervella J">J Kervella</name></author><author><name sortKey="Genard, M" uniqKey="Genard M">M Genard</name></author><author><name sortKey="Foulongne, M" uniqKey="Foulongne M">M Foulongne</name></author><author><name sortKey="Moreau, K" uniqKey="Moreau K">K Moreau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogundiwin, Ea" uniqKey="Ogundiwin E">EA Ogundiwin</name></author><author><name sortKey="Marti, C" uniqKey="Marti C">C Martí</name></author><author><name sortKey="Forment, J" uniqKey="Forment J">J Forment</name></author><author><name sortKey="Pons, C" uniqKey="Pons C">C Pons</name></author><author><name sortKey="Granell, A" uniqKey="Granell A">A Granell</name></author><author><name sortKey="Gradziel, Tm" uniqKey="Gradziel T">TM Gradziel</name></author><author><name sortKey="Peace, Cp" uniqKey="Peace C">CP Peace</name></author><author><name sortKey="Crisosto, Ch" uniqKey="Crisosto C">CH Crisosto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monet, R" uniqKey="Monet R">R Monet</name></author><author><name sortKey="Guye, A" uniqKey="Guye A">A Guye</name></author><author><name sortKey="Roy, M" uniqKey="Roy M">M Roy</name></author><author><name sortKey="Dachary, N" uniqKey="Dachary N">N Dachary</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Etienne, C" uniqKey="Etienne C">C Etienne</name></author><author><name sortKey="Moing, A" uniqKey="Moing A">A Moing</name></author><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Raymond, P" uniqKey="Raymond P">P Raymond</name></author><author><name sortKey="Monet, R" uniqKey="Monet R">R Monet</name></author><author><name sortKey="Rothan, C" uniqKey="Rothan C">C Rothan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peace, Cp" uniqKey="Peace C">CP Peace</name></author><author><name sortKey="Crisosto, Ch" uniqKey="Crisosto C">CH Crisosto</name></author><author><name sortKey="Garner, Dt" uniqKey="Garner D">DT Garner</name></author><author><name sortKey="Dandekar, Am" uniqKey="Dandekar A">AM Dandekar</name></author><author><name sortKey="Gradziel, Tm" uniqKey="Gradziel T">TM Gradziel</name></author><author><name sortKey="Bliss, Fa" uniqKey="Bliss F">FA Bliss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogundiwin, Ea" uniqKey="Ogundiwin E">EA Ogundiwin</name></author><author><name sortKey="Peace, Cp" uniqKey="Peace C">CP Peace</name></author><author><name sortKey="Gradziel, Tm" uniqKey="Gradziel T">TM Gradziel</name></author><author><name sortKey="Dandekar, Am" uniqKey="Dandekar A">AM Dandekar</name></author><author><name sortKey="Bliss, Fa" uniqKey="Bliss F">FA Bliss</name></author><author><name sortKey="Crisosto, Ch" uniqKey="Crisosto C">CH Crisosto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lopukhina, A" uniqKey="Lopukhina A">A Lopukhina</name></author><author><name sortKey="Dettenberg, M" uniqKey="Dettenberg M">M Dettenberg</name></author><author><name sortKey="Weiler, Ew" uniqKey="Weiler E">EW Weiler</name></author><author><name sortKey="Hollander Czytko, H" uniqKey="Hollander Czytko H">H Hollander-Czytko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Trainotti, L" uniqKey="Trainotti L">L Trainotti</name></author><author><name sortKey="Zanin, D" uniqKey="Zanin D">D Zanin</name></author><author><name sortKey="Casadoro, G" uniqKey="Casadoro G">G Casadoro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bouvier, F" uniqKey="Bouvier F">F Bouvier</name></author><author><name sortKey="D Harlingue, A" uniqKey="D Harlingue A">A D'Harlingue</name></author><author><name sortKey="Backhaus, Ra" uniqKey="Backhaus R">RA Backhaus</name></author><author><name sortKey="Kumagai, Mh" uniqKey="Kumagai M">MH Kumagai</name></author><author><name sortKey="Camara, B" uniqKey="Camara B">B Camara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marin Rodriguez, Mc" uniqKey="Marin Rodriguez M">MC Marin-Rodriguez</name></author><author><name sortKey="Orchard, J" uniqKey="Orchard J">J Orchard</name></author><author><name sortKey="Seymour, Gb" uniqKey="Seymour G">GB Seymour</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tipping, Aj" uniqKey="Tipping A">AJ Tipping</name></author><author><name sortKey="Mcpherson, Mj" uniqKey="Mcpherson M">MJ McPherson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luan, S" uniqKey="Luan S">S Luan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Moing, A" uniqKey="Moing A">A Moing</name></author><author><name sortKey="Rothan, C" uniqKey="Rothan C">C Rothan</name></author><author><name sortKey="Svanella, L" uniqKey="Svanella L">L Svanella</name></author><author><name sortKey="Pronier, V" uniqKey="Pronier V">V Pronier</name></author><author><name sortKey="Guye, A" uniqKey="Guye A">A Guye</name></author><author><name sortKey="Plomion, C" uniqKey="Plomion C">C Plomion</name></author><author><name sortKey="Monet, R" uniqKey="Monet R">R Monet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miclet, E" uniqKey="Miclet E">E Miclet</name></author><author><name sortKey="Stoven, V" uniqKey="Stoven V">V Stoven</name></author><author><name sortKey="Michels, Pam" uniqKey="Michels P">PAM Michels</name></author><author><name sortKey="Opperdoes, Fr" uniqKey="Opperdoes F">FR Opperdoes</name></author><author><name sortKey="Lallemand, J V" uniqKey="Lallemand J">J-V Lallemand</name></author><author><name sortKey="Duffieux, F" uniqKey="Duffieux F">F Duffieux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ishiguro, S" uniqKey="Ishiguro S">S Ishiguro</name></author><author><name sortKey="Nakamura, K" uniqKey="Nakamura K">K Nakamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Downs, Cg" uniqKey="Downs C">CG Downs</name></author><author><name sortKey="Somerfield, Sd" uniqKey="Somerfield S">SD Somerfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goldsmith Fischman, S" uniqKey="Goldsmith Fischman S">S Goldsmith-Fischman</name></author><author><name sortKey="Kuzin, A" uniqKey="Kuzin A">A Kuzin</name></author><author><name sortKey="Edstrom, Wc" uniqKey="Edstrom W">WC Edstrom</name></author><author><name sortKey="Benach, J" uniqKey="Benach J">J Benach</name></author><author><name sortKey="Shastry, R" uniqKey="Shastry R">R Shastry</name></author><author><name sortKey="Xiao, R" uniqKey="Xiao R">R Xiao</name></author><author><name sortKey="Acton, Tb" uniqKey="Acton T">TB Acton</name></author><author><name sortKey="Honig, B" uniqKey="Honig B">B Honig</name></author><author><name sortKey="Montelione, Gt" uniqKey="Montelione G">GT Montelione</name></author><author><name sortKey="Hunt, Jf" uniqKey="Hunt J">JF Hunt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Milewski, S" uniqKey="Milewski S">S Milewski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kader, Aa" uniqKey="Kader A">AA Kader</name></author><author><name sortKey="Chordas, A" uniqKey="Chordas A">A Chordas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="O Neill, Sd" uniqKey="O Neill S">SD O'Neill</name></author><author><name sortKey="Tong, Y" uniqKey="Tong Y">Y Tong</name></author><author><name sortKey="Sporlein, B" uniqKey="Sporlein B">B Sporlein</name></author><author><name sortKey="Forkmann, G" uniqKey="Forkmann G">G Forkmann</name></author><author><name sortKey="Yoder, Ji" uniqKey="Yoder J">JI Yoder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bonghi, C" uniqKey="Bonghi C">C Bonghi</name></author><author><name sortKey="Rascio, N" uniqKey="Rascio N">N Rascio</name></author><author><name sortKey="Ramina, A" uniqKey="Ramina A">A Ramina</name></author><author><name sortKey="Casadoro, G" uniqKey="Casadoro G">G Casadoro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martin, T" uniqKey="Martin T">T Martin</name></author><author><name sortKey="Frommer, Wb" uniqKey="Frommer W">WB Frommer</name></author><author><name sortKey="Salanoubat, M" uniqKey="Salanoubat M">M Salanoubat</name></author><author><name sortKey="Willmitzer, L" uniqKey="Willmitzer L">L Willmitzer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cunningham, Fx" uniqKey="Cunningham F">FX Cunningham</name></author><author><name sortKey="Sun, Z" uniqKey="Sun Z">Z Sun</name></author><author><name sortKey="Chamovitz, D" uniqKey="Chamovitz D">D Chamovitz</name></author><author><name sortKey="Hirschberg, J" uniqKey="Hirschberg J">J Hirschberg</name></author><author><name sortKey="Grantt, E" uniqKey="Grantt E">E Grantt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalaitzis, P" uniqKey="Kalaitzis P">P Kalaitzis</name></author><author><name sortKey="Solomos, T" uniqKey="Solomos T">T Solomos</name></author><author><name sortKey="Tucket, Ml" uniqKey="Tucket M">ML Tucket</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hong, Sb" uniqKey="Hong S">SB Hong</name></author><author><name sortKey="Tucker, Ml" uniqKey="Tucker M">ML Tucker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, S" uniqKey="Chang S">S Chang</name></author><author><name sortKey="Tan, C" uniqKey="Tan C">C Tan</name></author><author><name sortKey="Frankel, En" uniqKey="Frankel E">EN Frankel</name></author><author><name sortKey="Barrett, Dm" uniqKey="Barrett D">DM Barrett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ruperti, B" uniqKey="Ruperti B">B Ruperti</name></author><author><name sortKey="Bonghi, C" uniqKey="Bonghi C">C Bonghi</name></author><author><name sortKey="Rasori, A" uniqKey="Rasori A">A Rasori</name></author><author><name sortKey="Ramina, A" uniqKey="Ramina A">A Ramina</name></author><author><name sortKey="Tonutti, P" uniqKey="Tonutti P">P Tonutti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamada, K" uniqKey="Yamada K">K Yamada</name></author><author><name sortKey="Niwa, N" uniqKey="Niwa N">N Niwa</name></author><author><name sortKey="Shiratake, K" uniqKey="Shiratake K">K Shiratake</name></author><author><name sortKey="Yamaki, S" uniqKey="Yamaki S">S Yamaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jaakola, L" uniqKey="Jaakola L">L Jaakola</name></author><author><name sortKey="Maatta, K" uniqKey="Maatta K">K Maatta</name></author><author><name sortKey="Pirttila, An" uniqKey="Pirttila A">AN Pirttila</name></author><author><name sortKey="Torronen, R" uniqKey="Torronen R">R Torronen</name></author><author><name sortKey="Karenlampi, S" uniqKey="Karenlampi S">S Karenlampi</name></author><author><name sortKey="Hohtola, A" uniqKey="Hohtola A">A Hohtola</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fridman, E" uniqKey="Fridman E">E Fridman</name></author><author><name sortKey="Zamir, D" uniqKey="Zamir D">D Zamir</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Iyidogan, Nf" uniqKey="Iyidogan N">NF Iyidogan</name></author><author><name sortKey="Bayindirli, A" uniqKey="Bayindirli A">A Bayindirli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Jones, L" uniqKey="Jones L">L Jones</name></author><author><name sortKey="Mcqueen Mason, S" uniqKey="Mcqueen Mason S">S McQueen-Mason</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Trainotti, L" uniqKey="Trainotti L">L Trainotti</name></author><author><name sortKey="Pavanello, A" uniqKey="Pavanello A">A Pavanello</name></author><author><name sortKey="Zanin, D" uniqKey="Zanin D">D Zanin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bagnoli, F" uniqKey="Bagnoli F">F Bagnoli</name></author><author><name sortKey="Danti, S" uniqKey="Danti S">S Danti</name></author><author><name sortKey="Magherini, V" uniqKey="Magherini V">V Magherini</name></author><author><name sortKey="Cozza, R" uniqKey="Cozza R">R Cozza</name></author><author><name sortKey="Innocenti, Am" uniqKey="Innocenti A">AM Innocenti</name></author><author><name sortKey="Racchi, Ml" uniqKey="Racchi M">ML Racchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, Z" uniqKey="Wu Z">Z Wu</name></author><author><name sortKey="Burns, Jk" uniqKey="Burns J">JK Burns</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dal Cin, V" uniqKey="Dal Cin V">V Dal Cin</name></author><author><name sortKey="Danesin, M" uniqKey="Danesin M">M Danesin</name></author><author><name sortKey="Boschetti, A" uniqKey="Boschetti A">A Boschetti</name></author><author><name sortKey="Dorigoni, A" uniqKey="Dorigoni A">A Dorigoni</name></author><author><name sortKey="Ramina, A" uniqKey="Ramina A">A Ramina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chaves, Als" uniqKey="Chaves A">ALS Chaves</name></author><author><name sortKey="De Mello Farias, Pc" uniqKey="De Mello Farias P">PC de Mello-Farias</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tieman, D" uniqKey="Tieman D">D Tieman</name></author><author><name sortKey="Taylor, M" uniqKey="Taylor M">M Taylor</name></author><author><name sortKey="Schauer, N" uniqKey="Schauer N">N Schauer</name></author><author><name sortKey="Fernie, Ar" uniqKey="Fernie A">AR Fernie</name></author><author><name sortKey="Hanson, Ad" uniqKey="Hanson A">AD Hanson</name></author><author><name sortKey="Klee, Hj" uniqKey="Klee H">HJ Klee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dirlewanger, E" uniqKey="Dirlewanger E">E Dirlewanger</name></author><author><name sortKey="Cosson, P" uniqKey="Cosson P">P Cosson</name></author><author><name sortKey="Tavaud, M" uniqKey="Tavaud M">M Tavaud</name></author><author><name sortKey="Aranzana, Mj" uniqKey="Aranzana M">MJ Aranzana</name></author><author><name sortKey="Poizat, C" uniqKey="Poizat C">C Poizat</name></author><author><name sortKey="Zanetto, A" uniqKey="Zanetto A">A Zanetto</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author><author><name sortKey="Laigret, R" uniqKey="Laigret R">R Laigret</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aranzana, Mj" uniqKey="Aranzana M">MJ Aranzana</name></author><author><name sortKey="Garcia Mas, J" uniqKey="Garcia Mas J">J Garcia-Mas</name></author><author><name sortKey="Carbo, J" uniqKey="Carbo J">J Carbo</name></author><author><name sortKey="Arus, P" uniqKey="Arus P">P Arús</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sosinski, B" uniqKey="Sosinski B">B Sosinski</name></author><author><name sortKey="Gannavarapu, M" uniqKey="Gannavarapu M">M Gannavarapu</name></author><author><name sortKey="Hager, Ld" uniqKey="Hager L">LD Hager</name></author><author><name sortKey="Beck, Le" uniqKey="Beck L">LE Beck</name></author><author><name sortKey="King, Gj" uniqKey="King G">GJ King</name></author><author><name sortKey="Ryder, Cd" uniqKey="Ryder C">CD Ryder</name></author><author><name sortKey="Rajapakse, S" uniqKey="Rajapakse S">S Rajapakse</name></author><author><name sortKey="Baird, Wv" uniqKey="Baird W">WV Baird</name></author><author><name sortKey="Ballard, Re" uniqKey="Ballard R">RE Ballard</name></author><author><name sortKey="Abbott, Ag" uniqKey="Abbott A">AG Abbott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cantini, C" uniqKey="Cantini C">C Cantini</name></author><author><name sortKey="Iezzoni, Af" uniqKey="Iezzoni A">AF Iezzoni</name></author><author><name sortKey="Lamboy, Wf" uniqKey="Lamboy W">WF Lamboy</name></author><author><name sortKey="Boritzki, M" uniqKey="Boritzki M">M Boritzki</name></author><author><name sortKey="Struss, D" uniqKey="Struss D">D Struss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Struss, D" uniqKey="Struss D">D Struss</name></author><author><name sortKey="Ahmad, R" uniqKey="Ahmad R">R Ahmad</name></author><author><name sortKey="Southwick, Sm" uniqKey="Southwick S">SM Southwick</name></author><author><name sortKey="Boritzki, M" uniqKey="Boritzki M">M Boritzki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cipriani, G" uniqKey="Cipriani G">G Cipriani</name></author><author><name sortKey="Lot, G" uniqKey="Lot G">G Lot</name></author><author><name sortKey="Huang, Wg" uniqKey="Huang W">WG Huang</name></author><author><name sortKey="Marrazzo, Mt" uniqKey="Marrazzo M">MT Marrazzo</name></author><author><name sortKey="Peterlunger, E" uniqKey="Peterlunger E">E Peterlunger</name></author><author><name sortKey="Testolin, R" uniqKey="Testolin R">R Testolin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Testolin, R" uniqKey="Testolin R">R Testolin</name></author><author><name sortKey="Marrazzo, T" uniqKey="Marrazzo T">T Marrazzo</name></author><author><name sortKey="Cipriani, G" uniqKey="Cipriani G">G Cipriani</name></author><author><name sortKey="Quarta, R" uniqKey="Quarta R">R Quarta</name></author><author><name sortKey="Verde, I" uniqKey="Verde I">I Verde</name></author><author><name sortKey="Dettori, Mt" uniqKey="Dettori M">MT Dettori</name></author><author><name sortKey="Pancaldi, M" uniqKey="Pancaldi M">M Pancaldi</name></author><author><name sortKey="Sansavini, S" uniqKey="Sansavini S">S Sansavini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Testolin, R" uniqKey="Testolin R">R Testolin</name></author><author><name sortKey="Messina, R" uniqKey="Messina R">R Messina</name></author><author><name sortKey="Lain, O" uniqKey="Lain O">O Lain</name></author><author><name sortKey="Marrazzo, Mt" uniqKey="Marrazzo M">MT Marrazzo</name></author><author><name sortKey="Huang, Wg" uniqKey="Huang W">WG Huang</name></author><author><name sortKey="Cipriani, G" uniqKey="Cipriani G">G Cipriani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waldron, J" uniqKey="Waldron J">J Waldron</name></author><author><name sortKey="Peace, Cp" uniqKey="Peace C">CP Peace</name></author><author><name sortKey="Searle, Ir" uniqKey="Searle I">IR Searle</name></author><author><name sortKey="Furtado, A" uniqKey="Furtado A">A Furtado</name></author><author><name sortKey="Wade, N" uniqKey="Wade N">N Wade</name></author><author><name sortKey="Graham, Mw" uniqKey="Graham M">MW Graham</name></author><author><name sortKey="Carroll, Bj" uniqKey="Carroll B">BJ Carroll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Michelmore, Rw" uniqKey="Michelmore R">RW Michelmore</name></author><author><name sortKey="Paran, I" uniqKey="Paran I">I Paran</name></author><author><name sortKey="Kesseli, Rv" uniqKey="Kesseli R">RV Kesseli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ahmad, R" uniqKey="Ahmad R">R Ahmad</name></author><author><name sortKey="Potter, D" uniqKey="Potter D">D Potter</name></author><author><name sortKey="Southwick, Sm" uniqKey="Southwick S">SM Southwick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rozen, S" uniqKey="Rozen S">S Rozen</name></author><author><name sortKey="Skaletsky, Hj" uniqKey="Skaletsky H">HJ Skaletsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Ooijen, Jw" uniqKey="Van Ooijen J">JW Van Ooijen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kosambi, Dd" uniqKey="Kosambi D">DD Kosambi</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Genomics</journal-id><journal-title-group><journal-title>BMC Genomics</journal-title></journal-title-group><issn pub-type="epub">1471-2164</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19995417</article-id><article-id pub-id-type="pmc">2797820</article-id><article-id pub-id-type="publisher-id">1471-2164-10-587</article-id><article-id pub-id-type="doi">10.1186/1471-2164-10-587</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research article</subject></subj-group></article-categories><title-group><article-title>A fruit quality gene map of <italic>Prunus</italic></article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes" id="A1"><name><surname>Ogundiwin</surname><given-names>Ebenezer A</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>ogundiwin@gmail.com</email></contrib><contrib contrib-type="author" id="A2"><name><surname>Peace</surname><given-names>Cameron P</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>cpeace@wsu.edu</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Gradziel</surname><given-names>Thomas M</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>tmgradziel@ucdavis.edu</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Parfitt</surname><given-names>Dan E</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>deparfitt@ucdavis.edu</email></contrib><contrib contrib-type="author" id="A5"><name><surname>Bliss</surname><given-names>Fredrick A</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>fbliss@dcn.org</email></contrib><contrib contrib-type="author" id="A6"><name><surname>Crisosto</surname><given-names>Carlos H</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>carlos@uckac.edu</email></contrib></contrib-group><aff id="I1"><label>1</label>Plant Sciences Department, University of California Davis, 1 Shields Ave., Davis CA 95616, USA</aff><aff id="I2"><label>2</label>Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA 99164 USA</aff><pub-date pub-type="collection"><year>2009</year></pub-date><pub-date pub-type="epub"><day>8</day><month>12</month><year>2009</year></pub-date><volume>10</volume><fpage>587</fpage><lpage>587</lpage><history><date date-type="received"><day>16</day><month>3</month><year>2009</year></date><date date-type="accepted"><day>8</day><month>12</month><year>2009</year></date></history><permissions><copyright-statement>Copyright ©2009 Ogundiwin et al; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2009</copyright-year><copyright-holder>Ogundiwin et al; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.biomedcentral.com/1471-2164/10/587"></self-uri><abstract><sec><title>Background</title><p><italic>Prunus </italic>fruit development, growth, ripening, and senescence includes major biochemical and sensory changes in texture, color, and flavor. The genetic dissection of these complex processes has important applications in crop improvement, to facilitate maximizing and maintaining stone fruit quality from production and processing through to marketing and consumption. Here we present an integrated fruit quality gene map of <italic>Prunus </italic>containing 133 genes putatively involved in the determination of fruit texture, pigmentation, flavor, and chilling injury resistance.</p></sec><sec><title>Results</title><p>A genetic linkage map of 211 markers was constructed for an intraspecific peach (<italic>Prunus persica</italic>) progeny population, Pop-DG, derived from a canning peach cultivar 'Dr. Davis' and a fresh market cultivar 'Georgia Belle'. The Pop-DG map covered 818 cM of the peach genome and included three morphological markers, 11 ripening candidate genes, 13 cold-responsive genes, 21 novel EST-SSRs from the ChillPeach database, 58 previously reported SSRs, 40 RAFs, 23 SRAPs, 14 IMAs, and 28 accessory markers from candidate gene amplification. The Pop-DG map was co-linear with the <italic>Prunus </italic>reference T × E map, with 39 SSR markers in common to align the maps. A further 158 markers were bin-mapped to the reference map: 59 ripening candidate genes, 50 cold-responsive genes, and 50 novel EST-SSRs from ChillPeach, with deduced locations in Pop-DG via comparative mapping. Several candidate genes and EST-SSRs co-located with previously reported major trait loci and quantitative trait loci for chilling injury symptoms in Pop-DG.</p></sec><sec><title>Conclusion</title><p>The candidate gene approach combined with bin-mapping and availability of a community-recognized reference genetic map provides an efficient means of locating genes of interest in a target genome. We highlight the co-localization of fruit quality candidate genes with previously reported fruit quality QTLs. The fruit quality gene map developed here is a valuable tool for dissecting the genetic architecture of fruit quality traits in <italic>Prunus </italic>crops.</p></sec></abstract></article-meta></front><body><sec><title>Background</title><p>Molecular genetic linkage maps have become a major tool in genetics, genomics and breeding of plant and animal species. Linkage maps provide opportunities for unlocking the complex genetics of quantitatively inherited traits through the localization of quantitative trait loci (QTL), identification and positional cloning of individual genes, development of genome-wide physical maps, assembly and annotation of whole genome sequence, and serve as a repository of markers useful in marker-assisted breeding (MAB) of crop and animal species. Among the most informative maps for MAB are those constructed using parent genotypes directly involved in breeding programs.</p><p>Peach is one of the best genetically characterized species in the Rosaceae family [<xref ref-type="bibr" rid="B1">1</xref>,<xref ref-type="bibr" rid="B2">2</xref>], and the most economically important crop in <italic>Prunus </italic>[<xref ref-type="bibr" rid="B1">1</xref>], a genus that also includes nectarine, plum, apricot, cherry, and almond. The small genome size and expanding genomic resources of peach highlight peach as a model species for genomics studies of tree fruits [<xref ref-type="bibr" rid="B1">1</xref>-<xref ref-type="bibr" rid="B4">4</xref>]. Details of these genetic and genomic resources are updated and described on the Genomic Database for Rosaceae (GDR) [<xref ref-type="bibr" rid="B5">5</xref>].</p><p>While numerous <italic>Prunus </italic>species linkage maps have been published, the interspecific linkage map (T × E) developed from an interspecific cross of almond ("Texas") with peach ("Earlygold") is the most saturated of all these linkage maps [<xref ref-type="bibr" rid="B6">6</xref>-<xref ref-type="bibr" rid="B8">8</xref>]. Due to this saturation, a high degree of polymorphism, and extensive co-linearity and synteny among <italic>Prunus </italic>genomes [<xref ref-type="bibr" rid="B9">9</xref>,<xref ref-type="bibr" rid="B10">10</xref>], research community consensus has established the T × E map as the reference map for all <italic>Prunus </italic>species. The most recent published version of the T × E map contains 562 markers spanning 519 cM with an average density of 0.9 cM per marker [<xref ref-type="bibr" rid="B8">8</xref>]. Building on the reference status of T × E, a bin-mapping strategy was developed [<xref ref-type="bibr" rid="B11">11</xref>]. In this technique, recombination patterns in six progeny of the T × E mapping population were used to reduce the <italic>Prunus </italic>genome to 67 "bins" of 7.8 cM average length and to further populate the reference map with an additional 264 microsatellite-derived markers [<xref ref-type="bibr" rid="B11">11</xref>]. Other interspecific <italic>Prunus </italic>linkage maps were derived from almond 'Padre' × peach selection 54P545 [<xref ref-type="bibr" rid="B12">12</xref>,<xref ref-type="bibr" rid="B13">13</xref>], and myrobalan plum clone P.2175 × almond-peach hybrid clone GN22 [<xref ref-type="bibr" rid="B14">14</xref>]. Interspecific maps are easily saturated with markers due to the high level of polymorphism between parent genotypes. However, they are limited in their immediate applicability to cultivar improvement via MAS when compared to intraspecific maps because markers that are polymorphic between species are often not polymorphic within species. This is especially true for peach which has a narrow genetic base [<xref ref-type="bibr" rid="B15">15</xref>]. Reported intraspecific <italic>Prunus </italic>linkage maps include those of almond [<xref ref-type="bibr" rid="B16">16</xref>-<xref ref-type="bibr" rid="B18">18</xref>], apricot [<xref ref-type="bibr" rid="B19">19</xref>-<xref ref-type="bibr" rid="B22">22</xref>], sweet cherry [<xref ref-type="bibr" rid="B23">23</xref>], and peach [<xref ref-type="bibr" rid="B15">15</xref>,<xref ref-type="bibr" rid="B24">24</xref>-<xref ref-type="bibr" rid="B29">29</xref>]. The ultimate stated goal of most linkage map construction efforts for <italic>Prunus </italic>crop species is the development of breeder-friendly MAB tools. Potential benefits of MAB are particularly great for these crop species because of their long juvenility and requirements for large field planting spaces.</p><p>The concept of fruit quality of <italic>Prunus </italic>fruit crops includes both its attainment, such changes in color, flavor, and texture as fruit develop, grow, and ripen, and its maintenance following harvest from the tree as the perishable tissues senesce. <italic>Prunus </italic>fruit development, growth, ripening, and senescence includes major biochemical and sensory changes in texture, color, and flavor. The genetic dissection of these complex processes has important applications in crop improvement, to facilitate maximizing and maintaining stone fruit quality from production and processing through to marketing and consumption.</p><p>The goal of the present study was to develop a genomic resource to facilitate the genetic dissection of <italic>Prunus </italic>fruit quality traits. This paper reports the genetic mapping in the <italic>Prunus </italic>genome of candidate genes for fruit texture, pigmentation, flavor, and cold-responsiveness of peach, using both an intraspecific peach population to create a linkage map for genetic analyses of fruit quality and chilling injury (CI), and the interspecific <italic>Prunus </italic>reference map. The utility of the "fruit quality gene map" developed here for <italic>Prunus </italic>is demonstrated by highlighting co-localization of fruit quality QTLs with mapped fruit quality candidate genes.</p></sec><sec><title>Results</title><sec><title>Morphological markers</title><p>Peach blossom petals can be large and showy or small and curved on margins (non-showy). Non-showy is dominant to showy [<xref ref-type="bibr" rid="B30">30</xref>]. 'Dr. Davis' and 'Georgia Belle' are both heterozygous and therefore non-showy for this locus. Pop-DG progeny segregated as 115 non-showy: 37 showy, fitting the expected Mendelian ratio of 3:1 (<italic>χ</italic><sup>2 </sup>= 0.04; <italic>P </italic>= 0.98). This trait was designated <italic>Sh </italic>and mapped to the middle of linkage group G8 of Pop-DG and flanked by SSR marker CPPCT006 at 5 cM above and CI resistance CG marker <italic>Unk5 </italic>at 11.2 cM below. Segregation and mapping of the peach mesocarp color (<italic>Y</italic>) and the freestone melting flesh (<italic>F-M</italic>) loci have been reported previously for Pop-DG [<xref ref-type="bibr" rid="B31">31</xref>,<xref ref-type="bibr" rid="B32">32</xref>].</p></sec><sec><title>Molecular marker polymorphism in Pop-DG</title><p>Three types of reproducible marker polymorphism were observed on the PAGE profiles of the CG PCR products: fragment size polymorphism of the targeted gene fragments, additional markers that were designated as "CG accessory markers" generated elsewhere in the profile than the main CG fragments, and single strand conformation polymorphism (SSCP). Under the conditions used for PAGE, some reproducible sharp or shadowy banding patterns were observed in association with the target PCR product. We have proved this to be mobility shifts characteristic of SSCP resulting from SNPs within the amplicons [<xref ref-type="bibr" rid="B33">33</xref>]. The SSCP phenomenon also occurred for many SSRs (Figure <xref ref-type="fig" rid="F1">1</xref>).</p><fig id="F1" position="float"><label>Figure 1</label><caption><p><bold>Polyacrylamide gel profile of SSR marker BPPCT036</bold>. The gel profile shows the re-assignment of BPPCT036 from linkage group G4 (bin 4:63) to bin 1:73 on the <italic>Prunus </italic>T × E reference map.</p></caption><graphic xlink:href="1471-2164-10-587-1"></graphic></fig><p>Of all 229 SSR primer pairs screened for polymorphism in Pop-DG, 68 (~30%) were polymorphic and produced 79 SSR markers (Figure <xref ref-type="fig" rid="F2">2</xref>). Polymorphism was higher in the 133 genomic SSRs (37%) than in the 96 EST-SSRs (23%). Of the 76 novel ChillPeach EST-SSRs tested in Pop-DG, 17 (22%) were polymorphic, on par with the public EST-SSR polymorphism. The remaining 59 ChillPeach EST-SSRs were screened on the T × E bin set, out of which 51 (86%) were polymorphic. Approximately 13% and 18% polymorphism was obtained for CI resistance CGs and other CGs in Pop-DG, respectively, while 71% and 86% polymorphism was obtained in T × E, respectively (Figure <xref ref-type="fig" rid="F2">2</xref>). Marker polymorphism in Pop-DG was therefore at least four to five times less than in T × E for all classes of marker tested.</p><fig id="F2" position="float"><label>Figure 2</label><caption><p><bold>Level of polymorphism of three molecular marker types in Pop-DG and T × E</bold>. The intraspecific peach population (Pop-DG) compared with the interspecific <italic>Prunus </italic>population (T × E); SSR = simple sequence repeat, CG = fruit ripening candidate genes, CIRG = CI resistance genes.</p></caption><graphic xlink:href="1471-2164-10-587-2"></graphic></fig></sec><sec><title>Pop-DG linkage map</title><p>The Pop-DG intraspecific peach linkage map contained a total of 211 markers (208 molecular and three morphological) distributed over eight linkage groups corresponding to the haploid chromosome number of peach (Figure <xref ref-type="fig" rid="F3">3</xref>). The map covered 818.2 cM of the peach genome with an average of 4.0 cM interval between markers. The markers on Pop-DG map consisted of three Mendelian trait loci, 24 CGs, 79 SSRs, 40 RAFs, 23 SRAPs, 14 IMAs, and 28 CG accessory markers associated with CGs. Of the 79 SSR markers on Pop-DG, 39 were shared with the published <italic>Prunus </italic>T × E reference map (Figure <xref ref-type="fig" rid="F3">3</xref>). These common markers enabled the determination of linkage group orientation and assignment of linkage group numbers for the Pop-DG map. Shared markers were co-linear between Pop-DG and T × E except in three cases. Marker positions for BPPCT024, BPPCT030, and pchgms1 were inverted at the lower end of linkage group G2 of Pop-DG compared to G2 of T × E, positions of BPPCT021 and UDP96-008 were inverted in the middle of G3 of Pop-DG compared to G3 of T × E, and positions of BPPCT026 and CPPCT004 were inverted towards the upper end of G5 of Pop-DG compared to G5 of T × E. One SSR marker (BPPCT036) that was originally placed on linkage group G4 of T × E [<xref ref-type="bibr" rid="B7">7</xref>] mapped to the distal end of G1 in Pop-DG. To resolve this discrepancy, BPPCT036 was tested on the T × E bin set which confirmed its true location in bin 1:73 (Figure <xref ref-type="fig" rid="F1">1</xref>), corresponding to its position on the Pop-DG linkage map.</p><fig id="F3" position="float"><label>Figure 3</label><caption><p><bold>Genetic linkage map of Pop-DG with fruit texture, flavor, pigment, and CI resistance genes</bold>. Pop-DG = 'Dr. Davis × 'Georgia Belle'. Open vertical bars represent linkage groups. Vertical solid bars represent linkage groups of the T × E <italic>Prunus </italic>reference map (Dirlewanger et al. 2004; Howard et al. 2005) showing the bins and anchored with linkage groups of Pop-DG. Positions of SSR markers on the T × E map corresponding to the Pop-DG map are connected by dotted lines. Genetic markers are to the right side of each linkage group of Pop-DG, genetic distances (cM) are to the left. Markers in bold are fruit texture, pigment, flavor, and CI resistance candidate genes. Markers with prefix 'C-' are novel <italic>Prunus </italic>EST-SSRs obtained from the ChillPeach database (Ogundiwin et al. 2008). RAF and SRAP markers start with prefixes 'r' and 's', respectively. Accessory markers are italicized. Markers in blue fonts were heterozygous in 'Georgia Belle' only, markers in red fonts were heterozygous in 'Dr. Davis' only, and all other markers were heterozygous in both parents.</p></caption><graphic xlink:href="1471-2164-10-587-3"></graphic></fig><p>The features of CGs mapped to Pop-DG are presented in Table <xref ref-type="table" rid="T1">1</xref>. Eleven markers were derived from fruit quality-related CGs (Table <xref ref-type="table" rid="T1">1</xref>), seven of which were texture CGs. One of these texture CGs, <italic>endoPG </italic>(endopolygalacturonase), mapped to linkage group G4 as previously reported (Peace et al. 2005a). The others were two pectin methylesterases (<italic>PME1 </italic>&<italic>PME5</italic>) on G1 and G7, another polygalacturonase (<italic>PG4</italic>) on G8, pectate lyase (<italic>PL2</italic>) on G1, alpha-L-arabinofuranosidase (<italic>Ara</italic>) on G5, and a MADS box transcription factor similar to tomato ripening inhibitor (<italic>RIN</italic>) on G4. Three pigmentation CGs mapped to Pop-DG. These were beta-carotene hydroxylase (<italic>BCH</italic>) on G2, leucoanthocyanidin dioxygenase (<italic>PpLDOX</italic>) on G5 as previously reported by Ogundiwin et al. (2008), and zeaxanthin epoxidase (<italic>ZXE2</italic>) on G7. A flavor CG for sucrose phosphate synthase (<italic>SPS</italic>) mapped to G1. Thirteen mapped Pop-DG gene markers were obtained from CI resistance CGs. Seven of these have functional annotation: Aux/IAA protein (<italic>Aux-IAA</italic>) on G1, chloroplast nucleoid DNA binding (<italic>CND</italic>) on G1, thaumatin-like protein1 precursor (<italic>TP1</italic>) on G3, serine protease-like protein (<italic>SPP</italic>) on G7, tonoplast intrinsic protein (<italic>TIP</italic>) on G7, indole-3-acetic acid-induced protein ARG2 (<italic>Arg2</italic>) on G8, and sulfate transporter (<italic>ST</italic>) on G8. The other six had no functional annotation (labeled with the prefix "Unk" for "unknown") on G1, G2, G3, G8, and two on G6. Some dominant markers were generated from accessory fragments amplified alongside major amplicons of a few candidate genes (See Additional file <xref ref-type="supplementary-material" rid="S1">1</xref> - Table S1). Seventeen of such markers were mapped to Pop-DG.</p><table-wrap id="T1" position="float"><label>Table 1</label><caption><p>Features of candidate and cold responsive genes mapped to Pop-DG</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="center">LG</th><th align="left">Marker Code</th><th align="left">Functional Annotation</th><th align="left">Clone/Accession #</th><th align="left">EST Source</th><th align="left">CG type<sup>a</sup></th></tr></thead><tbody><tr><td align="center">1</td><td align="center">PL2</td><td align="left">Pectate lyase</td><td align="left">BU041363</td><td align="left">GDR</td><td align="left">Texture</td></tr><tr><td></td><td align="center">Unk23</td><td align="left">similar to F19P19.4 protein related cluster</td><td align="left">PP1004A08-T7_c_s</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td></td><td align="center">PME1</td><td align="left">pectinesterase, putative</td><td align="left">BU043277</td><td align="left">GDR</td><td align="left">Texture</td></tr><tr><td></td><td align="center">CND</td><td align="left">Chloroplast nucleoid DNA binding protein related cluster</td><td align="left">PPN018D10-T7_c_s</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td></td><td align="center">SPS</td><td align="left">Sucrose phosphate synthase</td><td align="left">DY653691</td><td align="left">GDR</td><td align="left">Flavor</td></tr><tr><td></td><td align="center">Aux-IAA</td><td align="left">Aux/IAA protein related cluster</td><td align="left">CL78Contig1</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td colspan="6"><hr></hr></td></tr><tr><td align="center">2</td><td align="center">BCH</td><td align="left">Beta-carotene-hydroxylase</td><td align="left">BU044761</td><td align="left">GDR</td><td align="left">Pigment</td></tr><tr><td></td><td align="center">Unk20</td><td align="left">OSJNBb0039L24.13 protein</td><td align="left">CL1095Contig1</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td colspan="6"><hr></hr></td></tr><tr><td align="center">3</td><td align="center">Unk13</td><td align="left">highly similar to OSJNBb0004A17.4 protein related cluster</td><td align="left">CL32Contig2</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td></td><td align="center">TP1</td><td align="left">Thaumatin-like protein 1 precursor</td><td align="left">PPN003H07-T7_c_s</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td colspan="6"><hr></hr></td></tr><tr><td align="center">4</td><td align="center">RIN</td><td align="left">Similar to <italic>Solanum lycopersicum </italic>MADS-RIN MADS box transcription factor</td><td align="left">BU045116</td><td align="left">GDR</td><td align="left">Texture</td></tr><tr><td></td><td align="center">endoPG</td><td align="left">endopolygalacturonase</td><td align="left">BU040689</td><td align="left">GDR</td><td align="left">Texture</td></tr><tr><td colspan="6"><hr></hr></td></tr><tr><td align="center">5</td><td align="center">PpLDOX</td><td align="left">Leucoanthocyanidin dioxygenase</td><td align="left">EU292217</td><td align="left">Ogundiwin et al., 2008</td><td align="left">Pigment</td></tr><tr><td></td><td align="center">Ara</td><td align="left">Alpha-L-arabinofuranosidase</td><td align="left">DQ486870</td><td align="left">NCBI</td><td align="left">Texture</td></tr><tr><td colspan="6"><hr></hr></td></tr><tr><td align="center">6</td><td align="center">Unk10</td><td align="left">No annotation available</td><td align="left">PP1005B10-T7_c_s</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td></td><td align="center">Unk19</td><td align="left">No annotation available</td><td align="left">PPN024C05-T7_c_s</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td colspan="6"><hr></hr></td></tr><tr><td align="center">7</td><td align="center">SPP</td><td align="left">Serine protease-like protein related cluster</td><td align="left">PPN007C09-T7_c_s</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td></td><td align="center">PME5</td><td align="left">pectin methylesterase - like protein</td><td align="left">BU044844</td><td align="left">GDR</td><td align="left">Texture</td></tr><tr><td></td><td align="center">TIP</td><td align="left">Tonoplast intrinsic protein related cluster</td><td align="left">PP1003C07-T7_c_s</td><td align="left">ChillPeach</td><td align="left">ChillPeach</td></tr><tr><td></td><td align="center">ZXE2</td><td align="left">Zeaxanthin epoxidase</td><td align="left">CL377Contig1</td><td align="left">ChillPeach</td><td align="left">Pigment</td></tr><tr><td colspan="6"><hr></hr></td></tr><tr><td align="center">8</td><td align="center">Unk5</td><td align="left">No annotation available</td><td align="left">PP1004F11-T7_c_s</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td></td><td align="center">PG4</td><td align="left"><italic>P. persica </italic>PG gene</td><td align="left">X77231</td><td align="left">NCBI</td><td align="left">Texture</td></tr><tr><td></td><td align="center">ST</td><td align="left">Sulfate transporter 3.1</td><td align="left">PPN065F08-T7_c_s</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr><tr><td></td><td align="center">Arg2</td><td align="left">Indole-3-acetic acid-induced protein ARG2 related cluster</td><td align="left">CL704Contig1</td><td align="left">ChillPeach</td><td align="left">CIRG</td></tr></tbody></table><table-wrap-foot><p><sup>a</sup>: CIRG = chilling injury resistance genes</p></table-wrap-foot></table-wrap><p>Features of the 18 ChillPeach ESTs that produced 21 EST-SSRs mapped to Pop-DG are provided (See Additional file <xref ref-type="supplementary-material" rid="S2">2</xref> - Table S2). Eight of these have annotated functions: two zinc finger-RING type, thioredoxin domain 2, POZ/BTB containing protein, biotin synthase, lysine ketoglutarate reductase, transfactor-like protein, TRNA intron endonuclease, and farnesyltransferase beta subunit. Other ChillPeach EST-SSRs on Pop-DG have no known functional annotation.</p></sec><sec><title>Bin-mapping CGs and ChillPeach EST-SSRs to the <italic>Prunus </italic>reference T × E map</title><p>The bin-mapping technique developed for the <italic>Prunus </italic>T × E reference map enabled the mapping of 158 markers that were monomorphic in Pop-DG (Figure <xref ref-type="fig" rid="F4">4</xref>; See Additional file <xref ref-type="supplementary-material" rid="S3">3</xref> - Table S3). These included 109 CGs and 49 novel ChillPeach EST-SSRs. The bin-mapped CGs included 35 texture and nine pigmentation CGs. All markers mapped to 53 of the 67 T × E bins. Approximately 30 of the 50 bin-mapped ChillPeach EST-SSR markers (60%) were derived from ESTs with known GO annotations (See Additional file <xref ref-type="supplementary-material" rid="S2">2</xref> - Table S2). Others were unknown.</p><fig id="F4" position="float"><label>Figure 4</label><caption><p><bold>Candidate genes (CGs) and novel <italic>Prunus </italic>EST-SSR markers bin-mapped to the <italic>Prunus </italic>T × E reference map</bold>. CI resistance CGs are in bold fonts, texture CGs are underlined, CGs related to fruit pigmentation are italicized, other CGs are asterisked, and new <italic>Prunus </italic>EST-SSRs are in normal font.</p></caption><graphic xlink:href="1471-2164-10-587-4"></graphic></fig></sec></sec><sec><title>Discussion</title><p>We have developed a detailed fruit quality and ripening gene map for <italic>Prunus</italic>. The fruit quality gene map contains 133 candidate genes (CGs) implicated in fruit ripening, softening, flavor, and pigmentation, and chilling injury resistance. The Pop-DG peach map is almost entirely co-linear with the <italic>Prunus </italic>reference T × E map such that locations of markers and quantitative trait loci (QTLs) located on Pop-DG can be readily cross-referenced to T × E and other <italic>Prunus </italic>maps aligned to T × E. Similarly, markers and QTLs in other <italic>Prunus </italic>maps (and other Rosaceae crop maps as comparative genomics advances in this family) can be compared to the <italic>Prunus </italic>fruit quality gene map to identify genes controlling fruit ripening and sensory quality.</p><sec><title>Co-linearity between Pop-DG and T × E maps</title><p>Using a reference genetic map with available bin-mapping resources to map genes and other DNA sequences not polymorphic within crop-specific maps is a powerful means of identifying marker-trait associations. The degree of resolution offered by bin-mapping in T × E (1 to 30 cM bins) is at the same scale as typical QTL mapping, such that detection of co-location (and equally useful, lack of co-location) of candidate genes with previously mapped QTLs is readily achievable. The remarkable conservation of co-linearity among the genomes of <italic>Prunus </italic>species [<xref ref-type="bibr" rid="B8">8</xref>] was exploited by bin-mapping to the T × E reference map many CGs that were monomorphic in Pop-DG. The higher rate of polymorphism observed in T × E compared to Pop-DG is not surprising because T × E is an F<sub>2 </sub>population from an interspecific cross. Pop-DG's polymorphism results from a relatively high heterozygosity in 'Georgia Belle' compared to modern cultivars, low heterozygosity in 'Dr. Davis' arising from a pedigree of closely related yellow non-melting canning peaches, and divergent breeding histories of these two parent cultivars. We expect that intraspecific peach populations derived from modern cultivars within the same market type (fresh or canning) will display even less polymorphism than Pop-DG. Inversion of marker positions observed between Pop-DG and T × E in three locations (linkage groups G2, G3, and G5) were among marker pairs that were close together on the T × E map (< 10 cM), suggesting that they are more probably caused by errors in the assignment of marker order than to inversion of chromosome fragments [<xref ref-type="bibr" rid="B8">8</xref>].</p></sec><sec><title>Simply inherited traits mapped to Pop-DG</title><p>Markers on the Pop-DG linkage map included three simply-inherited Mendelian quality and morphological traits: freestone/clingstone and melting flesh/non-melting flesh (<italic>F-M</italic>), mesocarp color (<italic>Y</italic>), and non-showy flower petals (<italic>Sh</italic>). The <italic>Sh </italic>locus mapped to linkage group G8 of Pop-DG. This is the first report of its genomic location since its inheritance was elucidated in the early to mid 1940s. Confirmation of its location on G8 is emerging from some unpublished results (Shenghua Fan and Tatyana Zhebentyayeva, personal communication). This is the only Mendelian trait mapped to G8 of <italic>Prunus </italic>to date. Linkage group G8 has proved difficult to map in some intraspecific peach mapping efforts [<xref ref-type="bibr" rid="B29">29</xref>,<xref ref-type="bibr" rid="B34">34</xref>]. In this report also, G8 was the linkage group with fewest number of markers. This could partly explain why it took so long to determine the genomic location of the <italic>Sh </italic>locus. The <italic>F-M </italic>(on linkage group G4) and <italic>Y </italic>(on linkage group G1) loci are among the list of 28 simply-inherited Mendelian traits mapped to <italic>Prunus </italic>genome in prior studies [<xref ref-type="bibr" rid="B8">8</xref>], and their locations are confirmed in Pop-DG.</p></sec><sec><title>Novel <italic>Prunus </italic>EST-SSR markers</title><p>Seventy new <italic>Prunus </italic>EST-SSR markers were mapped either directly to Pop-DG (21 markers) or bin-mapped to the T × E reference map (49 markers). The markers were obtained from the ChillPeach EST database [<xref ref-type="bibr" rid="B35">35</xref>]. The ChillPeach database is a specialized collection of ESTs from peach mesocarp tissue subjected to cold storage and ripening. The new set of mapped EST-SSR markers provides additional resources for molecular marker analyses in <italic>Prunus </italic>species.</p></sec><sec><title>Identity of mapped CGs</title><p>CG sequence identities were confirmed for <italic>endoPG </italic>[<xref ref-type="bibr" rid="B32">32</xref>], <italic>PpLDOX </italic>[<xref ref-type="bibr" rid="B33">33</xref>], and <italic>RIN </italic>(data not shown), where fragment lengths and DNA sequence of amplicons corresponded to original EST sequences. The identity of remaining CGs was confirmed by fragment length, where the most intense amplicons were either the expected size (approximately two-thirds of CGs) or were 80-1300 bp larger presumably due to the inclusion of one or more introns (and confirmed for the aforementioned cases). Identity of CG amplicons was also supported in many cases by previously reported map locations (described below). Further validation via sequencing would be desirable for the remaining CGs, and is the next recommended step for pursuit of specific QTL candidates for fruit quality traits of interest.</p></sec><sec><title>Comparing locations of CGs bin-mapped on T × E to other <italic>Prunus </italic>maps</title><p>Several previously mapped fruit candidate genes in <italic>Prunus </italic>corresponded to their genome locations in the present study. The locations of three CGs bin-mapped to T × E in this study corresponded to a previous interspecific 'Padre' × 54P455 map [<xref ref-type="bibr" rid="B13">13</xref>]. Catalase was mapped as an isozyme locus (<italic>Cat1</italic>) to the top of linkage group G5 of the 'Padre' × 54P455 map. We bin-mapped this texture CG to T × E bin 5:04. In earlier studies, the peach fruit acidity locus (<italic>D</italic>) was mapped to the top of G5 [<xref ref-type="bibr" rid="B25">25</xref>] and a tight linkage between <italic>Cat1 </italic>and the <italic>D </italic>locus was also reported [<xref ref-type="bibr" rid="B36">36</xref>]. Another isozyme, isocitrate dehydrogenase, was mapped as <italic>ICD </italic>to the top of linkage group G3 of 'Padre' × 54P455 [<xref ref-type="bibr" rid="B13">13</xref>] and as Icdh1 to T × E [<xref ref-type="bibr" rid="B37">37</xref>], and we bin-mapped this flavor CG as <italic>ICDH </italic>to T × E bin 3:06. Dehydrin was mapped as <italic>DHN1 </italic>and <italic>Dehy2 </italic>to the middle of group G7 of 'Padre' × 54P455 [<xref ref-type="bibr" rid="B13">13</xref>] and T × E (in this report), respectively.</p><p>The gene encoding polygalacturonase inhibiting protein (<italic>PGIP</italic>) mapped to different locations in the two studies; it mapped to G7 in 'Padre' × 54P455 and to G6 on T × E. Different members of the <italic>PGIP </italic>gene family may have been mapped in the two separate studies. Another independent study bin-mapped the genes <italic>endoPG </italic>(as EPPCU1775) to bin 4:63, <italic>ACO1 </italic>(as MD206a) to 3:36, <italic>ACO3 </italic>(as MD205a) to 4:18, and <italic>PG4 </italic>(as MD207a) to 8:63 to T × E [<xref ref-type="bibr" rid="B11">11</xref>]. <italic>PG4 </italic>was also mapped as isozyme <italic>PG </italic>to the same end of G8 in an almond linkage map [<xref ref-type="bibr" rid="B16">16</xref>]. <italic>PEPC </italic>was mapped as <italic>PEPc </italic>in a 'Ferjalou Jalousia' × 'Fantasia' map [<xref ref-type="bibr" rid="B37">37</xref>] in the same region as our <italic>PEPC </italic>(3:22). These authors [<xref ref-type="bibr" rid="B37">37</xref>] also reported the location of another ten candidate genes for sweetness and acidity, and a different expansin gene to those mapped here, to various locations on a peach map using the T × E population. Three further fruit quality CGs were mapped in a separate study [<xref ref-type="bibr" rid="B11">11</xref>]: NADP dependent sorbitol 6-phosphate dehydrogenase (as MD201a) for sweetness to bin 8:19, H+ ATPase (as MD203a) for sweetness/acidity to bin 1:73, and endo-beta-1,4-glucanase for texture to bin 5:41 (a different gene family member to our <italic>EGase </italic>of bin 3:22). Additional fruit quality candidate genes have been located in the <italic>Prunus </italic>genome during hybridization-based efforts to physically map peach ESTs [<xref ref-type="bibr" rid="B4">4</xref>].</p></sec><sec><title>Co-locations of candidate genes and chilling injury QTLs</title><p>The co-linearity between the Pop-DG and T × E maps has begun to yield benefits in dissecting the genetic control of fruit quality traits in peach. We are using the fruit quality gene map to better understand the genetics of resistance to chilling injury, particularly focusing on the major symptoms of mealiness, browning, and bleeding. At least two cases of co-localizations of chilling injury QTLs and CGs mapped to Pop-DG/T × E were previously reported: <italic>endoPG </italic>on G4 with major QTLs for mealiness and bleeding [<xref ref-type="bibr" rid="B31">31</xref>,<xref ref-type="bibr" rid="B38">38</xref>,<xref ref-type="bibr" rid="B39">39</xref>] as well as to the <italic>Freestone-Melting flesh </italic>locus [<xref ref-type="bibr" rid="B31">31</xref>,<xref ref-type="bibr" rid="B32">32</xref>] and <italic>PpLDOX </italic>on G5 with cold storage-induced browning [<xref ref-type="bibr" rid="B33">33</xref>].</p><sec><title>Mealiness</title><p>A peach homolog of tomato MADS-RIN ripening inhibitor gene (<italic>RIN</italic>), necessary for fruit ripening in tomato, mapped close (6 cM) to <italic>endoPG</italic>. Two <italic>RIN </italic>accessory markers (<italic>rin-a </italic>and <italic>rin-b</italic>) also mapped distally to <italic>endoPG</italic>. The functional role of <italic>RIN </italic>in peach fruit ripening, mealiness, or bleeding has not yet been established. Four additional minor QTLs were reported for mealiness in Pop-DG corresponding approximately to T × E bins 4:18, 4:22-4:27, 4:28, and 6:80-6:84 [<xref ref-type="bibr" rid="B39">39</xref>]. We mapped one texture CG (<italic>ACO3</italic>) to bin 4:18, one CI resistance CG (<italic>Unk17</italic>) to bin 4:27, two CI resistance CGs (<italic>TAT </italic>and <italic>Unk28</italic>) to bin 4:28, and two CI resistance CGs (<italic>Unk15 </italic>and <italic>Unk24</italic>) to bins 6:80-6:84. <italic>ACO3 </italic>encodes 1-aminocyclopropane-1-carboxylate oxidase, a critical enzyme in ethylene biosynthesis. <italic>TAT </italic>(tyrosine aminotransferase) is an inducible protein in the plant methyl jasmonate defense system [<xref ref-type="bibr" rid="B40">40</xref>]. <italic>Unk15</italic>, <italic>Unk17</italic>, <italic>Unk24</italic>, and <italic>Unk28 </italic>are genes of unknown functions that were differentially regulated in cold-treated peach mesocarp tissue [<xref ref-type="bibr" rid="B35">35</xref>]. Each of these CGs are potential markers for mealiness resistance and warrant further investigation.</p></sec><sec><title>Flesh browning</title><p>Some CGs mapped to locations where QTLs have been reported for cold storage-induced flesh browning in peach fruit. A major browning QTL was located on linkage group G5 of Pop-DG corresponding to bin 5:21 of T × E [<xref ref-type="bibr" rid="B33">33</xref>,<xref ref-type="bibr" rid="B39">39</xref>]. <italic>PpLDOX </italic>(leucoanthocyanidin dioxygenase), which was initially mapped to this bin, was later fine-mapped to Pop-DG, and evidence of its association with browning was established [<xref ref-type="bibr" rid="B33">33</xref>]. The two minor browning QTLs reported on linkage group G2 of Pop-DG [<xref ref-type="bibr" rid="B39">39</xref>] correspond roughly to bins 2:08-2:13 and 2:20-2:25 of T × E map. Four ripening CGs (<italic>PL3</italic>, <italic>Gal2</italic>, <italic>Exp4</italic>, and <italic>ERP</italic>) and one pigmentation CG (<italic>NXCE</italic>) mapped to bin 2:08, and one CI resistance CG (<italic>O-6FAD</italic>) mapped to bin 2:13. Also one ripening CG (<italic>Unk3</italic>) and one CI resistance CG (<italic>RHB1</italic>) mapped to bin 2:25. <italic>PL3 </italic>(pectate lyase), <italic>Gal2 </italic>(beta-galactosidase), and <italic>EXP4 </italic>(expansin) are cell wall-degrading enzymes while <italic>ERP </italic>(ethylene-responsive small gtp-binding) is an ethylene-related protein. <italic>Unk3 </italic>is a protein of unknown function which was up-regulated by ethylene in peach mesocarp tissue [<xref ref-type="bibr" rid="B41">41</xref>]. <italic>NXCE </italic>(neoxanthin cleavage enzyme) is an abscisic acid biosynthesis gene that acts by oxidative cleavage of a carotenoid neoxanthin [<xref ref-type="bibr" rid="B42">42</xref>]. <italic>O-6FAD </italic>(omega-6 fatty acid desaturase) and <italic>RHB1 </italic>(RING-H2 finger protein) were up-regulated in cold-treated peach mesocarp tissue [<xref ref-type="bibr" rid="B35">35</xref>]. These CGs may be useful markers for developing resistance to cold-induced browning in stone fruit.</p></sec><sec><title>Cold-induced bleeding</title><p>In addition to the major QTL detected in peach fruit at the <italic>F-M</italic>/<italic>endoPG </italic>locus on G4 of Pop-DG, two minor QTLs were also reported for cold-induced bleeding on Pop-DG linkage group G1 [<xref ref-type="bibr" rid="B39">39</xref>], corresponding to T × E bins 1:14 and 1:34. A texture CG (<italic>PL2</italic>) and a CI resistance CG (<italic>Unk23</italic>) mapped close to the minor bleeding QTL peak at the top end of G1. Two texture CGs (<italic>PME3 </italic>and <italic>Ara2</italic>) and four CI resistance CGs (<italic>CIPK1</italic>, <italic>PDK2</italic>, <italic>C-CoAR</italic>, and <italic>Unk8</italic>) mapped to bin 1:14, while <italic>PCCAO </italic>(pigmentation CG) and <italic>Unk25 </italic>(CI resistance CG) mapped to bin 1:34. <italic>PL2 </italic>(pectate lyase), <italic>PME3 </italic>(pectin methylesterase), and <italic>Ara2 </italic>(alpha-L-arabinofuranosidase) are cell wall-degrading enzymes [<xref ref-type="bibr" rid="B41">41</xref>,<xref ref-type="bibr" rid="B43">43</xref>]. <italic>PPCAO </italic>(peroxisomal copper-containing amine oxidase) catalyzes the oxidation of amines to aldehyde, ammonia and hydrogen peroxide [<xref ref-type="bibr" rid="B44">44</xref>]. <italic>CIPK1 </italic>(calcineurin B-like protein-interacting protein kinase), involved in plant calcium signaling [<xref ref-type="bibr" rid="B45">45</xref>], <italic>PDK2 </italic>(pyruvate dehydogenase kinase), <italic>C-CoAR </italic>(cinnamoyl-CoA reductase), <italic>Unk8</italic>, and <italic>Unk25 </italic>were up-regulated in cold-treated mesocarp tissue of peach [<xref ref-type="bibr" rid="B35">35</xref>]. These CGs will be further investigated for possible roles in the formation of bleeding and developing bleeding-free peach cultivars.</p></sec></sec><sec><title>Inferred co-locations of candidate genes and other published fruit quality traits</title><p>Several CGs mapped to genomic regions corresponding to fruit quality QTLs reported in other studies. Examples are those reported by [<xref ref-type="bibr" rid="B34">34</xref>] and [<xref ref-type="bibr" rid="B46">46</xref>].</p><sec><title>Putative candidate genes for fruit quality QTLs on P1908 × 'Summergrand' map [<xref ref-type="bibr" rid="B34">34</xref>]</title><p>On linkage group G1 QTLs were detected by [<xref ref-type="bibr" rid="B34">34</xref>] for fructose (<italic>Fru</italic><sub>1,2</sub>), sweetness (<italic>Swe</italic><sub>2</sub>), quinic acid (<italic>Qui</italic><sub>1</sub>), fruit cheek diameter (<italic>FCheekD</italic><sub>2</sub>), fruit mass (<italic>FMass</italic><sub>2</sub>), and fruit suture diameter (<italic>FSutureD</italic><sub>2</sub>) in the region corresponding to the T × E bin 1:26 on which we bin-mapped <italic>Polyub </italic>(Polyubiquitin) and <italic>Unk6</italic>; QTLs for citric acid (<italic>Cit</italic><sub>1</sub>) and total sugar (<italic>TSugar</italic><sub>1</sub>) in the region equivalent to bin 1:50 of T × E on which we bin-mapped SAMDC (S-adenosylmethionine decarboxylase), <italic>Unk29</italic>, <italic>4CL </italic>(4-coumarate-CoA ligase-like protein), <italic>C-0472 </italic>(6-phosphogluconolactonase), <italic>C-PPN36E09</italic>, <italic>C-PPN73A08</italic>; QTLs for total sugar (<italic>TSugar</italic><sub>2</sub>) and quinic acid (<italic>Qui</italic><sub>2</sub>) in the region corresponding to bin 1:52-1:55 of T × E where we bin-mapped <italic>Chitb </italic>(Chitinase Ib), <italic>Unk9 </italic>and <italic>SREB </italic>(Sucrose-responsive element binding protein). Notable among these gene markers are those encoding sucrose-responsive element binding protein (<italic>SREB</italic>) and phosphogluconolactonase (<italic>C-0472</italic>) for their possible involvement in sugar biosynthesis [<xref ref-type="bibr" rid="B47">47</xref>] and inducible expression [<xref ref-type="bibr" rid="B48">48</xref>], respectively. In addition, [<xref ref-type="bibr" rid="B34">34</xref>] localized QTLs for sucrose (<italic>Suc</italic><sub>1</sub>, <italic>Suc</italic><sub>2</sub>), glucose (<italic>Glu</italic><sub>2</sub>), and quinic acid (<italic>Qui</italic><sub>1</sub>) on linkage group G7 region comparable to bin 7:56 of T × E containing bin-mapped CG <italic>AspS </italic>(Asparagine synthetase). The expression of <italic>AspS </italic>has been shown to increase with a decrease in sucrose levels [<xref ref-type="bibr" rid="B49">49</xref>].</p><p>The following QTLs were also detected for peach fruit quality on linkage group G4 by [<xref ref-type="bibr" rid="B34">34</xref>]: fruit mass (<italic>FMass</italic><sub>2</sub>), fruit polar diameter (<italic>FPolarD</italic><sub>2</sub>), soluble solid concentrate (<italic>SSC</italic><sub>1,2</sub>) and juiciness (<italic>Jui</italic><sub>2</sub>) on a region corresponding to bin 4:18 of T × E on which <italic>ACO3 </italic>and <italic>C-0212 </italic>(Acetyl Co-A acetyltransferase) were bin-mapped; and citric acid (<italic>Cit</italic><sub>2</sub>), quinic acid (<italic>Qui</italic><sub>2</sub>), total acid (<italic>TAcid</italic><sub>2</sub>), sorbitol (<italic>Sor</italic><sub>1,2</sub>), fructose (<italic>Fru</italic><sub>1</sub>) and malic acid (<italic>Mal</italic><sub>1</sub>) on a region equivalent to bin 4:27 - 4:28 of T × E on which were bin-mapped <italic>Unk17</italic>, <italic>Unk28</italic>, <italic>C-1077</italic>, and <italic>TAT </italic>(putative tyrosine aminotransferase). The authors also localized the following peach fruit quality QTLs on linkage group G5: glucose (<italic>Glu</italic><sub>2</sub>), fruit suture diameter (<italic>FSutureD</italic><sub>2</sub>), and fruit cheek diameter (<italic>FCheekD</italic><sub>2</sub>), as well as the major locus controlling fruit acidity, <italic>D</italic>, on a region corresponding approximately to bin 5:04 of T × E on which we bin-mapped <italic>PAE1</italic>, <italic>PAE2</italic>, and <italic>Cat1</italic>; red skin coloration (<italic>SRColor</italic><sub>2</sub>), dry flesh mass content (<italic>DFMC</italic><sub>1</sub>), soluble solid concentrate (<italic>SSC</italic><sub>1</sub>), fruit mass (<italic>FMass</italic><sub>1</sub>), fruit polar diameter (<italic>FPolarD</italic><sub>1,2</sub>), and fruit suture diameter (<italic>FSutureD</italic><sub>1</sub>) on bin 5:46-5:49 of T × E on which <italic>PL, C-PP03C02</italic>, and <italic>C-PPN05E11 </italic>(SufE-like protein) were bin-mapped. SulfE-related proteins have been implicated in Fe-S metabolism and export [<xref ref-type="bibr" rid="B50">50</xref>].</p></sec><sec><title>Putative candidate genes for fruit quality QTLs on 'Ferjalou Jalousia' × 'Fantasia' map [<xref ref-type="bibr" rid="B46">46</xref>]</title><p>A number of fruit quality QTLs (including fresh weight, sucrose, and SSC) were detected by [<xref ref-type="bibr" rid="B46">46</xref>] on linkage group G6 of 'Ferjalou Jalousia' × 'Fantasia' map, on the region corresponding to bin 6:74-6:84 of the T × E linkage map. To this bin, we mapped <italic>AGAT </italic>(Alanine--glyoxylate aminotransferase), <italic>CDTPL </italic>(C-terminal domain phosphatase-like), <italic>Sod4a </italic>(Superoxide dismutase), <italic>Unk5</italic>, <italic>Unk24</italic>, <italic>C-1182 </italic>(BZIP transcription factor bZIP105), <italic>C-1290</italic>, <italic>C-PPN12C07</italic>, <italic>C-PPN19B01</italic>, and <italic>C-PPN31E06 </italic>(Glutamine-fructose-6-phosphate transaminase). Glutamine-fructose-6-phosphate transaminase is an important enzyme in biosynthesis of amino sugar-containing macromolecules [<xref ref-type="bibr" rid="B51">51</xref>]. Also on linkage group G4, Dirlewanger et al (1999) localized QTLs for SSC and fructose to a region equivalent to the T × E bin 4:46. The candidate genes mapped to this bin were <italic>SeCy </italic>(Sesquiterpene cyclase), <italic>SAMM </italic>(S-adenosylmethionine:2-demethylmenaquinone methyltransferase), and <italic>GPPDE </italic>(glycerophosphoryl diester phosphodiesterase).</p></sec></sec></sec><sec><title>Conclusion</title><p>We have developed a fruit quality gene map for <italic>Prunus </italic>by determining the genomic locations of 133 fruit quality candidate genes with an intraspecific peach population, Pop-DG, and the <italic>Prunus </italic>reference map, T × E. Sufficient SSR marker anchoring between both maps allowed easy cross-referencing of marker and trait locus positions. We demonstrate here the use of this gene map for dissecting the molecular genetics of CI in peach. Using the results of microarray experiments that studied gene expression in cold-treated peach mesocarp tissue, 63 cold-responsive genes were located on the fruit quality gene map, allowing the detection of co-locations of these CI resistance CGs with QTLs for CI symptoms. We also highlight new CGs for previously reported <italic>Prunus </italic>QTLs of other fruit quality traits. The fruit quality gene map presented here is expected to be a valuable resource for the genetic analysis of fruit ripening and related fruit quality traits in <italic>Prunus </italic>species.</p></sec><sec sec-type="methods"><title>Methods</title><sec><title>Mapping population and T × E bin set</title><p>Pop-DG is a peach intraspecific cross between 'Dr. Davis' (female parent) and 'Georgia Belle' (pollen parent). 'Dr. Davis' is a modern canning peach cultivar while 'Georgia Belle' is a century-old fresh market peach cultivar. These cultivars contrast for many fruit quality and other traits (Table <xref ref-type="table" rid="T2">2</xref>, 3). Pop-DG, created and managed to study the genetics of fruit quality attainment and maintenance, particularly resistance to CI, in peach and nectarine, was established in two nearby orchards at Kearney Agricultural Center (Parlier, CA, USA). The first orchard was established in 1998 containing 51 verified hybrids [<xref ref-type="bibr" rid="B32">32</xref>]. Each progeny genotype was represented by two trees in the orchard; one tree planted on its own roots and the other tree on 'Nemaguard' rootstock. The second orchard was established in 2002, containing single trees of 277 true hybrid progeny on their own roots. All 51 progeny of the first orchard and 101 progeny of the second orchard (152 true hybrid progeny total) were used for Pop-DG linkage mapping. For bin-mapping in T × E, DNA samples of the bin set of the 'Texas' × 'Earlygold' population ('Earlygold' the F<sub>1 </sub>plant, and six F<sub>2 </sub>plants) [<xref ref-type="bibr" rid="B11">11</xref>], kindly provided by Dr. Werner Howad, were used. We also included the second parent - 'Texas' in each test.</p><table-wrap id="T2" position="float"><label>Table 2</label><caption><p>Fruit quality attributes of Pop-DG parent cultivars, 'Dr. Davis' and 'Georgia Belle'</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left">Trait<sup>a</sup></th><th align="left">'Dr. Davis'</th><th align="left">'Georgia Belle'</th></tr></thead><tbody><tr><td align="left">Ripening date</td><td align="left">Later</td><td align="left">Earlier</td></tr><tr><td align="left">Skin color</td><td align="left">Blush on orange ground</td><td align="left">Green/yellow, no blush</td></tr><tr><td align="left">Flesh color</td><td align="left">Yellow-orange</td><td align="left">White-cream</td></tr><tr><td align="left">Stone adhesion</td><td align="left">Clingstone</td><td align="left">Freestone</td></tr><tr><td align="left">Flesh texture</td><td align="left">Firm, non-melting flesh</td><td align="left">Soft, melting flesh</td></tr><tr><td align="left">Aroma</td><td align="left">Bland</td><td align="left">Sharp</td></tr><tr><td align="left">Sweetness (SSC)</td><td align="left">11.5</td><td align="left">13.0</td></tr><tr><td align="left">Acidity (TA)</td><td align="left">High</td><td align="left">Low</td></tr><tr><td align="left">Mealiness</td><td align="left">None</td><td align="left">High susceptibility</td></tr><tr><td align="left">Browning</td><td align="left">Medium susceptibility</td><td align="left">High susceptibility</td></tr><tr><td align="left">Bleeding</td><td align="left">High susceptibility</td><td align="left">Low susceptibility</td></tr></tbody></table><table-wrap-foot><p><sup>a</sup>: TA = titratable acidity, SSC = soluble solids concentrate</p></table-wrap-foot></table-wrap></sec><sec><title>Morphological markers</title><p>Pop-DG segregated for three Mendelian morphological traits: freestone melting flesh/clingstone non-melting flesh, white/yellow flesh color, and non-showy/showy flower petals. These traits were scored visually and included in linkage analysis alongside molecular markers.</p></sec><sec><title>Molecular markers</title><p>Various classes of molecular markers were evaluated. These marker classes consisted of genomic sequence-derived simple sequence repeats (SSR), expressed sequence tag [EST]-derived SSRs (EST-SSR), ethylene-related candidate genes (CG), texture CGs, pigmentation CGs, flavor CGs, CI resistance CGs, sequence-related amplified polymorphisms (SRAP), randomly amplified DNA fingerprinting (RAF), and inter-microsatellite amplification (IMA).</p><p>Candidate genes were nominated from published works and review articles on physiology and biochemistry of fruit ripening, softening, color (pigmentation), and flavor [<xref ref-type="bibr" rid="B37">37</xref>,<xref ref-type="bibr" rid="B41">41</xref>,<xref ref-type="bibr" rid="B43">43</xref>,<xref ref-type="bibr" rid="B52">52</xref>-<xref ref-type="bibr" rid="B71">71</xref>]. EST sequences of most of the CGs (including those described below) were obtained from the GDR database [<xref ref-type="bibr" rid="B5">5</xref>]. Others were obtained from the ChillPeach database [<xref ref-type="bibr" rid="B35">35</xref>] and GenBank.</p><p>CGs assembled for texture included those putatively encoding ethylene-related enzymes (e.g. aminocyclopropane-1-carboxylate synthases, aminocyclopropane-1-carboxylate oxidases, ethylene receptors, ethylene responsive element binding proteins, s-adenosyl-1-methionine synthases, peptide methionine sulfoxide redutase, and ripening inhibitor protein), although ethylene-related genes are also relevant for other fruit ripening processes. Other texture CGs were those putatively encoding cell wall-degrading enzymes (e.g. polygalacturonases, pectinesterases, pectate lyases, glucanases, mannosidases, xyloglucans, glycosylases and expansins). As indicated by [<xref ref-type="bibr" rid="B41">41</xref>], cell wall synthesis enzymes were also included (e.g glycosyltransferases and fiber protein enzymes), and from the same study, several genes of unknown function but strongly up-regulated by ethylene in ripening peach fruit were also included.</p><p>For pigmentation (skin and flesh color, including browning and bruising), candidate genes were chosen from the carotenoid and anthocyanin biosynthesis pathways (e.g. neoxanthin cleavage enzyme, leucoanthocyanidin dioxygenase, anthocyanidin-3-glucoside rhamnosyltransferase, beta-carotene hydroxylase, lycopene beta cyclase, peroxisomal copper containing amine oxidase, zeanthin epoxidase, geranylgeranyl pyrophosphate synthase, zeta carotene desaturase and phytoene desaturase). Genes encoding diphenol oxidases and polyphenol oxidases were also included.</p><p>Flavor CGs included sugar and acid biosynthesis pathway genes (e.g. sucrose synthase, hydroxyl methylglutaryl CoA reductase, cell wall invertase, sorbitol dehydrogenase, phosphoenolpyruvate carboxylase, chalcone synthase, polyketide synthase, alcohol dehydrogenase, and aromatic amino acid decarboxylase).</p><p>CI resistance CGs were cold-responsive genes obtained from the results of microarray analysis of cold-treated peach mesocarp tissues versus untreated mesocarp tissues [<xref ref-type="bibr" rid="B35">35</xref>]. Selection of CI resistance CGs were made as follows: 25 top performing genes, 24 genes common to peach and <italic>Arabidopsis thaliana </italic>(ChillPeach microarray data compared with ColdArrayDB: <ext-link ext-link-type="uri" xlink:href="http://cold.stanford.edu/cgi-bin/data.cgi">http://cold.stanford.edu/cgi-bin/data.cgi</ext-link>), and 39 genes unique to peach (i.e. not found in ColdArrayDB).</p><p>Published reports were the source of all genomic sequence SSRs (see below) and some EST-SSRs, while most EST-SSRs were newly obtained from the ChillPeach EST database [<xref ref-type="bibr" rid="B35">35</xref>]. A total of 153 published <italic>Prunus </italic>SSRs were screened for polymorphism between the Pop-DG mapping parents. These were <italic>P. persica </italic>SSRs with the prefix BPPCT [<xref ref-type="bibr" rid="B72">72</xref>], CPPCT [<xref ref-type="bibr" rid="B73">73</xref>], EPPCU (GDR database: [<xref ref-type="bibr" rid="B5">5</xref>]), Pchcms & Pchgms [<xref ref-type="bibr" rid="B74">74</xref>], PS [<xref ref-type="bibr" rid="B75">75</xref>], UCD-CH [<xref ref-type="bibr" rid="B76">76</xref>], and UDP [<xref ref-type="bibr" rid="B77">77</xref>,<xref ref-type="bibr" rid="B78">78</xref>], and <italic>P. dulcis </italic>SSRs with the prefix UDA [<xref ref-type="bibr" rid="B79">79</xref>]. The ChillPeach database provided 76 new EST-SSRs for screening, and the markers were labeled with the prefix 'C-' followed by the clone or contig number (e.g. C-PPN28F07 and C-1128).</p><p>RAF markers were obtained according to [<xref ref-type="bibr" rid="B80">80</xref>] protocols using Operon decamer primers AA18, B12, B15, D19, E02, E11, E16, P04, P07 and W06 after preliminary screening of many others via the bulked segregant analysis approach [<xref ref-type="bibr" rid="B81">81</xref>] with mealiness phenotypic extremes. SRAP marker analysis was conducted according to [<xref ref-type="bibr" rid="B82">82</xref>]. One IMA primer (IMA08: (GA)<sup>8</sup>GT; [<xref ref-type="bibr" rid="B25">25</xref>]) was also used to generate additional molecular markers for Pop-DG.</p></sec><sec><title>PCR and PAGE</title><p>All PCR primers were designed using Primer3 software [<xref ref-type="bibr" rid="B83">83</xref>]. EST sequences of CGs were examined for microsatellite motifs, and whenever ESTs were part of a contig, the contig was examined for SNPs and indels. Primers were designed to exploit these polymorphic features. Where these features were not observable, sequences at the 3'-end of the ESTs were used for designing primers to avoid long introns and target less-conserved 3' UTRs. Generally, primers were designed to limit expected amplicon size to ≤ 300 bp such that a PCR product even with an intron as long as 1 kb would still be observable on the large (50 × 38 cm) PAGE plate and 1 bp indels of intron-less amplified fragments could be detected. PCR and PAGE conditions were as reported in Peace et al. (2005b). Primer sequences, annealing temperatures, expected and observed amplicon sizes, and type of polymorphisms are provided (See Additional files <xref ref-type="supplementary-material" rid="S3">3</xref> and <xref ref-type="supplementary-material" rid="S4">4</xref>).</p></sec><sec><title>Map construction</title><p>Linkage analysis was conducted with JoinMap<sup>® </sup>4 [<xref ref-type="bibr" rid="B84">84</xref>]. Linkage parameters were set as 3.0 minimum LOD and 0.45 maximum recombination fraction. The Kosambi mapping function [<xref ref-type="bibr" rid="B85">85</xref>] was used to convert recombination fraction to map distances in centimorgans (cM). The marker data type was set as cross-pollination (CP). The bin-mapping procedure followed [<xref ref-type="bibr" rid="B11">11</xref>].</p></sec></sec><sec><title>Authors' contributions</title><p>EAO, CPP, TMG, FAB and CHC conceived the study. TMG, FAB and CHC generated and established the Pop-DG mapping population. EAO and CPP conducted molecular marker discovery and genotyping, and EAO carried out linkage analysis, map construction and manuscript preparation. DEP also assisted in data analysis. DEP, CPP and FAB provided a very thorough review of the manuscript. All authors read and approved the final manuscript.</p></sec><sec sec-type="supplementary-material"><title>Supplementary Material</title><supplementary-material content-type="local-data" id="S1"><caption><title>Additional file 1</title><p><bold>Table S1- Features of dominant amplicons (accessory markers) generated alongside the target PCR products of candidate and cold responsive genes mapped to Pop-DG</bold>. The data provided represent information on accession number, map location, and fragment size information of dominant amplicons (accessory markers) generated alongside the target PCR products of candidate and cold responsive genes mapped to Pop-DG.</p></caption><media xlink:href="1471-2164-10-587-S1.DOC" mimetype="text" mime-subtype="plain"><caption><p>Click here for file</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="S2"><caption><title>Additional file 2</title><p><bold>Table S2 - Characteristics of 71 new <italic>Prunus </italic>ChillPeach EST-SSR markers mapped to the peach/<italic>Prunus </italic>genome</bold>. The data provided represent information on unigene, map location, and functional annotation of 71 new <italic>Prunus </italic>ChillPeach EST-SSR markers mapped to the peach/<italic>Prunus </italic>genome.</p></caption><media xlink:href="1471-2164-10-587-S2.DOC" mimetype="text" mime-subtype="plain"><caption><p>Click here for file</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="S3"><caption><title>Additional file 3</title><p><bold>Table S3 - Characteristics of candidate genes (CGs) bin-mapped to the T × E reference <italic>Prunus </italic>map</bold>. The data provided represent information on the genome location (bin name), marker code, clone/accession number, source of ESTs, and CG class of all CGs bin-mapped to the T × E reference <italic>Prunus </italic>map.</p></caption><media xlink:href="1471-2164-10-587-S3.DOC" mimetype="text" mime-subtype="plain"><caption><p>Click here for file</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="S4"><caption><title>Additional file 4</title><p><bold>Table S4 - Primer details for candidate genes and EST-SSRs</bold>. The data provided represent information on the primer sequence, annealing temperature, amplicons size, and type of polymorphisms for candidate genes and EST-SSRs.</p></caption><media xlink:href="1471-2164-10-587-S4.DOC" mimetype="text" mime-subtype="plain"><caption><p>Click here for file</p></caption></media></supplementary-material></sec></body><back><sec><title>Acknowledgements</title><p>The assistance of Drs. Howad and Arús for supplying the T × E bin-set DNA samples is gratefully acknowledged. We thank Dr. Vanaja Konduri for early work on Pop-DG mapping, Dr. Riaz Ahmad for generating SRAP marker data, Josh Williamson for initial investigations of pigmentation CGs in Pop-DG, and Daniel Edge-Garza and Jose Soto for DNA extraction of most of Pop-DG. This research was supported by UC Discovery Grants (bio03-10394 & bio05-10527) with the Industry-University Cooperative Research Program; by Research Grant No US-4027-07 from BARD, the United States - Israel Binational Agricultural Research and Development Fund; and by California Tree Fruit Agreement.</p></sec><ref-list><ref id="B1"><mixed-citation publication-type="journal"><name><surname>Abbott</surname><given-names>A</given-names></name><name><surname>Georgi</surname><given-names>L</given-names></name><name><surname>Yvergniaux</surname><given-names>D</given-names></name><name><surname>Wang</surname><given-names>Y</given-names></name><name><surname>Blenda</surname><given-names>A</given-names></name><name><surname>Reighard</surname><given-names>G</given-names></name><name><surname>Inigo</surname><given-names>M</given-names></name><name><surname>Sosinski</surname><given-names>B</given-names></name><article-title>Peach: The model genome for Rosaceae</article-title><source>Acta Hort</source><year>2002</year><volume>575</volume><fpage>145</fpage><lpage>155</lpage></mixed-citation></ref><ref id="B2"><mixed-citation publication-type="journal"><name><surname>Shulaev</surname><given-names>V</given-names></name><name><surname>Korban</surname><given-names>SS</given-names></name><name><surname>Sosinski</surname><given-names>B</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><name><surname>Aldwinckle</surname><given-names>HS</given-names></name><name><surname>Folta</surname><given-names>KM</given-names></name><name><surname>Iezzoni</surname><given-names>A</given-names></name><name><surname>Main</surname><given-names>D</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><name><surname>Dandekar</surname><given-names>AM</given-names></name><name><surname>Lewers</surname><given-names>K</given-names></name><name><surname>Brown</surname><given-names>SK</given-names></name><name><surname>Davis</surname><given-names>TM</given-names></name><name><surname>Gardiner</surname><given-names>SE</given-names></name><name><surname>Potter</surname><given-names>D</given-names></name><name><surname>Veilleux</surname><given-names>RE</given-names></name><article-title>Multiple models for Rosaceae genomics</article-title><source>Plant Physiol</source><year>2008</year><volume>157</volume><fpage>985</fpage><lpage>1003</lpage><pub-id pub-id-type="doi">10.1104/pp.107.115618</pub-id></mixed-citation></ref><ref id="B3"><mixed-citation publication-type="journal"><name><surname>Byrne</surname><given-names>DH</given-names></name><article-title>Isozyme variability in four diploid stone fruits compared with other woody perennial plants</article-title><source>J Hered</source><year>1990</year><volume>81</volume><fpage>68</fpage><lpage>71</lpage></mixed-citation></ref><ref id="B4"><mixed-citation publication-type="journal"><name><surname>Zhebentyayeva</surname><given-names>TN</given-names></name><name><surname>Swire-Clark</surname><given-names>G</given-names></name><name><surname>Georgi</surname><given-names>LL</given-names></name><name><surname>Garay</surname><given-names>L</given-names></name><name><surname>Jung</surname><given-names>S</given-names></name><name><surname>Forrest</surname><given-names>S</given-names></name><name><surname>Blenda</surname><given-names>AV</given-names></name><name><surname>Blackmon</surname><given-names>B</given-names></name><name><surname>Mook</surname><given-names>J</given-names></name><name><surname>Horn</surname><given-names>R</given-names></name><name><surname>Howad</surname><given-names>W</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><name><surname>Main</surname><given-names>D</given-names></name><name><surname>Tomkins</surname><given-names>JP</given-names></name><name><surname>Sosinski</surname><given-names>B</given-names></name><name><surname>Baird</surname><given-names>WV</given-names></name><name><surname>Reighard</surname><given-names>GL</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><article-title>A framework physical map for the peach, a model Rosaceae species</article-title><source>Tree Genet Genomes</source><year>2008</year><volume>4</volume><fpage>745</fpage><lpage>756</lpage><pub-id pub-id-type="doi">10.1007/s11295-008-0147-z</pub-id></mixed-citation></ref><ref id="B5"><mixed-citation publication-type="journal"><name><surname>Jung</surname><given-names>S</given-names></name><name><surname>Staton</surname><given-names>M</given-names></name><name><surname>Lee</surname><given-names>T</given-names></name><name><surname>Blenda</surname><given-names>A</given-names></name><name><surname>Svancara</surname><given-names>R</given-names></name><name><surname>Abbott</surname><given-names>A</given-names></name><name><surname>Main</surname><given-names>D</given-names></name><article-title>GDR (Genome Database for Rosaceae): integrated web-database for Rosaceae genomics and genetics data</article-title><source>Nucleic Acids Res</source><year>2008</year><volume>36</volume><fpage>D1034</fpage><lpage>D1040</lpage><pub-id pub-id-type="doi">10.1093/nar/gkm803</pub-id><pub-id pub-id-type="pmid">17932055</pub-id></mixed-citation></ref><ref id="B6"><mixed-citation publication-type="journal"><name><surname>Joobeur</surname><given-names>T</given-names></name><name><surname>Viruel</surname><given-names>MA</given-names></name><name><surname>de Vicente</surname><given-names>MC</given-names></name><name><surname>Jauregui</surname><given-names>B</given-names></name><name><surname>Ballester</surname><given-names>J</given-names></name><name><surname>Dettori</surname><given-names>MT</given-names></name><name><surname>Verde</surname><given-names>I</given-names></name><name><surname>Truco</surname><given-names>MJ</given-names></name><name><surname>Messeguer</surname><given-names>R</given-names></name><name><surname>Battle</surname><given-names>I</given-names></name><name><surname>Quarta</surname><given-names>R</given-names></name><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><article-title>Construction of a saturated linkage map for <italic>Prunus </italic>using an almond × peach F2 progeny</article-title><source>Theor Appl Genet</source><year>1998</year><volume>97</volume><fpage>1034</fpage><lpage>1041</lpage><pub-id pub-id-type="doi">10.1007/s001220050988</pub-id></mixed-citation></ref><ref id="B7"><mixed-citation publication-type="journal"><name><surname>Aranzana</surname><given-names>MJ</given-names></name><name><surname>Pineda</surname><given-names>A</given-names></name><name><surname>Cosson</surname><given-names>P</given-names></name><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Ascasibar</surname><given-names>J</given-names></name><name><surname>Cipriani</surname><given-names>G</given-names></name><name><surname>Ryder</surname><given-names>CD</given-names></name><name><surname>Testolini</surname><given-names>R</given-names></name><name><surname>Abbott</surname><given-names>A</given-names></name><name><surname>King</surname><given-names>GJ</given-names></name><name><surname>Iezzoni</surname><given-names>AF</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><article-title>A set of simple-sequence repeat (SSR) markers covering the <italic>Prunus </italic>genome</article-title><source>Theor Appl Genet</source><year>2003</year><volume>106</volume><fpage>819</fpage><lpage>825</lpage><pub-id pub-id-type="pmid">12647055</pub-id></mixed-citation></ref><ref id="B8"><mixed-citation publication-type="journal"><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Graziano</surname><given-names>E</given-names></name><name><surname>Joobeur</surname><given-names>T</given-names></name><name><surname>Garriga-Caldere</surname><given-names>F</given-names></name><name><surname>Cosson</surname><given-names>P</given-names></name><name><surname>Howard</surname><given-names>W</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><article-title>Comparative mapping and marker assisted selection in Rosaceae fruit crops</article-title><source>Proc Natl Acad Sci USA</source><year>2004</year><volume>101</volume><fpage>9891</fpage><lpage>9896</lpage><pub-id pub-id-type="doi">10.1073/pnas.0307937101</pub-id><pub-id pub-id-type="pmid">15159547</pub-id></mixed-citation></ref><ref id="B9"><mixed-citation publication-type="book"><name><surname>Arús</surname><given-names>P</given-names></name><name><surname>Yamamoto</surname><given-names>T</given-names></name><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><person-group person-group-type="editor">Janick J</person-group><article-title>Synteny in the Rosaceae</article-title><source>Plant Breeding Reviews</source><year>2006</year><volume>27</volume><publisher-name>Hoboken: Wiley</publisher-name><fpage>172</fpage><lpage>211</lpage></mixed-citation></ref><ref id="B10"><mixed-citation publication-type="book"><name><surname>Abbott</surname><given-names>AG</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><name><surname>Scorza</surname><given-names>R</given-names></name><person-group person-group-type="editor">Kole C</person-group><article-title>Peach</article-title><source>Genome mapping and molecular breeding in plants</source><year>2006</year><publisher-name>Berlin: Springer</publisher-name><fpage>137</fpage><lpage>156</lpage></mixed-citation></ref><ref id="B11"><mixed-citation publication-type="journal"><name><surname>Howad</surname><given-names>W</given-names></name><name><surname>Yamamoto</surname><given-names>T</given-names></name><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Testolin</surname><given-names>R</given-names></name><name><surname>Cosson</surname><given-names>P</given-names></name><name><surname>Cipriani</surname><given-names>G</given-names></name><name><surname>Monforte</surname><given-names>AJ</given-names></name><name><surname>Georgi</surname><given-names>L</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><article-title>Mapping with a few plants: using selective mapping for microsatellite saturation of the <italic>Prunus </italic>reference map</article-title><source>Genetics</source><year>2005</year><volume>171</volume><fpage>1305</fpage><lpage>1309</lpage><pub-id pub-id-type="doi">10.1534/genetics.105.043661</pub-id><pub-id pub-id-type="pmid">16118196</pub-id></mixed-citation></ref><ref id="B12"><mixed-citation publication-type="journal"><name><surname>Foolad</surname><given-names>MR</given-names></name><name><surname>Arulsekar</surname><given-names>S</given-names></name><name><surname>Becerra</surname><given-names>V</given-names></name><name><surname>Bliss</surname><given-names>FA</given-names></name><article-title>A genetic map of <italic>Prunus </italic>based on an interspecific cross between peach and almond</article-title><source>Theor Appl Genet</source><year>1995</year><volume>91</volume><fpage>262</fpage><lpage>269</lpage><pub-id pub-id-type="doi">10.1007/BF00220887</pub-id></mixed-citation></ref><ref id="B13"><mixed-citation publication-type="journal"><name><surname>Bliss</surname><given-names>FA</given-names></name><name><surname>Arulsekar</surname><given-names>S</given-names></name><name><surname>Foolad</surname><given-names>MR</given-names></name><name><surname>Becerra</surname><given-names>V</given-names></name><name><surname>Gillen</surname><given-names>AM</given-names></name><name><surname>Warburton</surname><given-names>ML</given-names></name><name><surname>Dandekar</surname><given-names>AM</given-names></name><name><surname>Kocsisne</surname><given-names>GM</given-names></name><name><surname>Mydin</surname><given-names>KK</given-names></name><article-title>An expanded genetic linkage map of <italic>Prunus </italic>based on an interspecific cross between almond and peach</article-title><source>Genome</source><year>2002</year><volume>45</volume><fpage>520</fpage><lpage>529</lpage><pub-id pub-id-type="doi">10.1139/g02-011</pub-id><pub-id pub-id-type="pmid">12033621</pub-id></mixed-citation></ref><ref id="B14"><mixed-citation publication-type="journal"><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Cosson</surname><given-names>P</given-names></name><name><surname>Howad</surname><given-names>W</given-names></name><name><surname>Capdeville</surname><given-names>G</given-names></name><name><surname>Bosselut</surname><given-names>N</given-names></name><name><surname>Claverie</surname><given-names>M</given-names></name><name><surname>Voisin</surname><given-names>R</given-names></name><name><surname>Poizat</surname><given-names>C</given-names></name><name><surname>Lafargue</surname><given-names>B</given-names></name><name><surname>Baron</surname><given-names>O</given-names></name><name><surname>Laigret</surname><given-names>F</given-names></name><name><surname>Kleinhentz</surname><given-names>M</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><name><surname>Esmenjaud</surname><given-names>D</given-names></name><article-title>Microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid - location of root-knot nematode resistance genes</article-title><source>Theor Appl Genet</source><year>2004</year><volume>109</volume><fpage>827</fpage><lpage>838</lpage><pub-id pub-id-type="doi">10.1007/s00122-004-1694-9</pub-id><pub-id pub-id-type="pmid">15241595</pub-id></mixed-citation></ref><ref id="B15"><mixed-citation publication-type="journal"><name><surname>Rajapakse</surname><given-names>S</given-names></name><name><surname>Belthoff</surname><given-names>LE</given-names></name><name><surname>He</surname><given-names>G</given-names></name><name><surname>Estager</surname><given-names>AE</given-names></name><name><surname>Scorza</surname><given-names>R</given-names></name><name><surname>Verde</surname><given-names>I</given-names></name><name><surname>Ballard</surname><given-names>RE</given-names></name><name><surname>Baird</surname><given-names>WV</given-names></name><name><surname>Callahan</surname><given-names>A</given-names></name><name><surname>Monet</surname><given-names>R</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><article-title>Genetic linkage mapping in peach using morphological, RFLP and RAPD markers</article-title><source>Theor Appl Genet</source><year>1995</year><volume>90</volume><fpage>503</fpage><lpage>510</lpage><pub-id pub-id-type="doi">10.1007/BF00221996</pub-id></mixed-citation></ref><ref id="B16"><mixed-citation publication-type="journal"><name><surname>Viruel</surname><given-names>MA</given-names></name><name><surname>Messeguer</surname><given-names>R</given-names></name><name><surname>de Vicente</surname><given-names>MC</given-names></name><name><surname>Garcia-Mas</surname><given-names>J</given-names></name><name><surname>Puigdomenech</surname><given-names>P</given-names></name><name><surname>Vargas</surname><given-names>FJ</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><article-title>A linkage map with RFLP and isozyme markers for almond</article-title><source>Theor Appl Genet</source><year>1995</year><volume>91</volume><fpage>964</fpage><lpage>971</lpage><pub-id pub-id-type="doi">10.1007/BF00223907</pub-id></mixed-citation></ref><ref id="B17"><mixed-citation publication-type="journal"><name><surname>Joobeur</surname><given-names>T</given-names></name><name><surname>Periam</surname><given-names>N</given-names></name><name><surname>de Vicente</surname><given-names>MC</given-names></name><name><surname>King</surname><given-names>GJ</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><article-title>Development of a second generation linkage map for almond using RAPD and SSR markers</article-title><source>Genome</source><year>2000</year><volume>43</volume><fpage>649</fpage><lpage>688</lpage><pub-id pub-id-type="doi">10.1139/gen-43-4-649</pub-id><pub-id pub-id-type="pmid">10984177</pub-id></mixed-citation></ref><ref id="B18"><mixed-citation publication-type="journal"><name><surname>Sánchez-Pérez</surname><given-names>R</given-names></name><name><surname>Howad</surname><given-names>W</given-names></name><name><surname>Dicenta</surname><given-names>F</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><name><surname>Martínez-Gómez</surname><given-names>P</given-names></name><article-title>Mapping major genes and quantitative trait loci controlling agronomic traits in almond</article-title><source>Plant Breed</source><year>2007</year><volume>126</volume><fpage>310</fpage><lpage>319</lpage><pub-id pub-id-type="doi">10.1111/j.1439-0523.2007.01329.x</pub-id></mixed-citation></ref><ref id="B19"><mixed-citation publication-type="journal"><name><surname>Hurtado</surname><given-names>MA</given-names></name><name><surname>Vilanova</surname><given-names>S</given-names></name><name><surname>Romero</surname><given-names>C</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><name><surname>Llacer</surname><given-names>G</given-names></name><name><surname>Badenes</surname><given-names>ML</given-names></name><article-title>Genetic linkage maps of two apricot cultivars (<italic>Prunus armeniaca </italic>L.) based on molecular markers</article-title><source>Theor Appl Genet</source><year>2002</year><volume>105</volume><fpage>182</fpage><lpage>191</lpage><pub-id pub-id-type="doi">10.1007/s00122-002-0936-y</pub-id><pub-id pub-id-type="pmid">12582518</pub-id></mixed-citation></ref><ref id="B20"><mixed-citation publication-type="journal"><name><surname>Vilanova</surname><given-names>S</given-names></name><name><surname>Romero</surname><given-names>C</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><name><surname>Llacer</surname><given-names>G</given-names></name><name><surname>Badenes</surname><given-names>ML</given-names></name><article-title>An apricot (<italic>Prunus armeniaca </italic>L.) F<sub>2 </sub>progeny linkage map based on SSR and AFLP markers, mapping plum pox virus resistance and self-incompatibility traits</article-title><source>Theor Appl Genet</source><year>2003</year><volume>107</volume><fpage>239</fpage><lpage>247</lpage><pub-id pub-id-type="doi">10.1007/s00122-003-1243-y</pub-id><pub-id pub-id-type="pmid">12845439</pub-id></mixed-citation></ref><ref id="B21"><mixed-citation publication-type="journal"><name><surname>Lambert</surname><given-names>P</given-names></name><name><surname>Hagen</surname><given-names>LS</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><name><surname>Audergon</surname><given-names>JM</given-names></name><article-title>Genetic linkage maps of two apricot cultivars (<italic>Prunus armeniaca </italic>L.) compared with the almond 'Texas' × peach 'Earlygold' reference map for <italic>Prunus</italic></article-title><source>Theor Appl Genet</source><year>2004</year><volume>108</volume><fpage>1120</fpage><lpage>1130</lpage><pub-id pub-id-type="doi">10.1007/s00122-003-1526-3</pub-id><pub-id pub-id-type="pmid">15067399</pub-id></mixed-citation></ref><ref id="B22"><mixed-citation publication-type="journal"><name><surname>Dondini</surname><given-names>L</given-names></name><name><surname>Lain</surname><given-names>O</given-names></name><name><surname>Geuna</surname><given-names>F</given-names></name><name><surname>Banfi</surname><given-names>R</given-names></name><name><surname>Gaiotti</surname><given-names>F</given-names></name><name><surname>Tartarini</surname><given-names>S</given-names></name><name><surname>Bassi</surname><given-names>D</given-names></name><name><surname>Testolin</surname><given-names>R</given-names></name><article-title>Development of a new SSR-based linkage map in apricot and analysis of synteny with existing <italic>Prunus </italic>maps</article-title><source>Tree Genet Genomes</source><year>2007</year><volume>3</volume><fpage>239</fpage><lpage>249</lpage><pub-id pub-id-type="doi">10.1007/s11295-006-0059-8</pub-id></mixed-citation></ref><ref id="B23"><mixed-citation publication-type="journal"><name><surname>Olmstead</surname><given-names>JW</given-names></name><name><surname>Sebolt</surname><given-names>AM</given-names></name><name><surname>Cabrera</surname><given-names>A</given-names></name><name><surname>Sooriyapathirana</surname><given-names>SH</given-names></name><name><surname>Iriarte</surname><given-names>G</given-names></name><name><surname>Wang</surname><given-names>D</given-names></name><name><surname>Chen</surname><given-names>CY</given-names></name><name><surname>Knaap</surname><given-names>E van der</given-names></name><name><surname>Iezzoni</surname><given-names>AF</given-names></name><article-title>Construction of an intra-specific sweet cherry (<italic>Prunus avium </italic>L.) genetic linkage map and synteny analysis with the Prunus reference map</article-title><source>Tree Genet Genomes</source><year>2008</year><volume>4</volume><fpage>897</fpage><lpage>910</lpage><pub-id pub-id-type="doi">10.1007/s11295-008-0161-1</pub-id></mixed-citation></ref><ref id="B24"><mixed-citation publication-type="journal"><name><surname>Chaparro</surname><given-names>JX</given-names></name><name><surname>Werner</surname><given-names>DJ</given-names></name><name><surname>O'Malley</surname><given-names>D</given-names></name><name><surname>Sederoff</surname><given-names>RR</given-names></name><article-title>Targeted mapping and linkage analysis of morphological, isozyme, and RAPD markers in peach</article-title><source>Theor Appl Genet</source><year>1994</year><volume>87</volume><fpage>805</fpage><lpage>815</lpage><pub-id pub-id-type="doi">10.1007/BF00221132</pub-id></mixed-citation></ref><ref id="B25"><mixed-citation publication-type="journal"><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Pronier</surname><given-names>V</given-names></name><name><surname>Parvery</surname><given-names>C</given-names></name><name><surname>Rothan</surname><given-names>C</given-names></name><name><surname>Guye</surname><given-names>A</given-names></name><name><surname>Monet</surname><given-names>R</given-names></name><article-title>A genetic linkage map of peach (<italic>Prunus persica </italic>L. Batsch) using morphological, RFLP, isoenzyme, RAPD, and AFLP markers</article-title><source>Theor Appl Genet</source><year>1998</year><volume>97</volume><fpage>888</fpage><lpage>895</lpage><pub-id pub-id-type="doi">10.1007/s001220050969</pub-id></mixed-citation></ref><ref id="B26"><mixed-citation publication-type="journal"><name><surname>Abbott</surname><given-names>AG</given-names></name><name><surname>Rajapakse</surname><given-names>S</given-names></name><name><surname>Sosinski</surname><given-names>B</given-names></name><name><surname>Lu</surname><given-names>ZX</given-names></name><name><surname>Sossey-Alaoui</surname><given-names>K</given-names></name><name><surname>Gannavarapu</surname><given-names>M</given-names></name><name><surname>Reighard</surname><given-names>G</given-names></name><name><surname>Ballard</surname><given-names>RE</given-names></name><name><surname>Baird</surname><given-names>WV</given-names></name><name><surname>Scorza</surname><given-names>R</given-names></name><name><surname>Callahan</surname><given-names>A</given-names></name><article-title>Construction of saturated linkage maps of peach crosses segregating for characters controlling fruit quality, tree architecture and pest resistance</article-title><source>Acta Hort</source><year>1998</year><volume>465</volume><fpage>41</fpage><lpage>49</lpage></mixed-citation></ref><ref id="B27"><mixed-citation publication-type="journal"><name><surname>Lu</surname><given-names>Z-X</given-names></name><name><surname>Sosinski</surname><given-names>B</given-names></name><name><surname>Reighard</surname><given-names>GL</given-names></name><name><surname>Baird</surname><given-names>WV</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><article-title>Construction of a genetic linkage map and identification of AFLP markers or resistance to root-knot nematodes in peach rootstocks</article-title><source>Genome</source><year>1998</year><volume>41</volume><fpage>199</fpage><lpage>207</lpage><pub-id pub-id-type="doi">10.1139/gen-41-2-199</pub-id></mixed-citation></ref><ref id="B28"><mixed-citation publication-type="journal"><name><surname>Yamamoto</surname><given-names>T</given-names></name><name><surname>Shimada</surname><given-names>T</given-names></name><name><surname>Imai</surname><given-names>T</given-names></name><name><surname>Yaegaki</surname><given-names>H</given-names></name><name><surname>Haji</surname><given-names>T</given-names></name><name><surname>Matsuta</surname><given-names>N</given-names></name><name><surname>Yamaguchi</surname><given-names>M</given-names></name><name><surname>Hayashi</surname><given-names>T</given-names></name><article-title>Characterization of morphological traits based on a genetic linkage map in peach</article-title><source>Breed Sci</source><year>2001</year><volume>51</volume><fpage>271</fpage><lpage>278</lpage><pub-id pub-id-type="doi">10.1270/jsbbs.51.271</pub-id></mixed-citation></ref><ref id="B29"><mixed-citation publication-type="journal"><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Cosson</surname><given-names>P</given-names></name><name><surname>Boudehri</surname><given-names>K</given-names></name><name><surname>Renaud</surname><given-names>C</given-names></name><name><surname>Capdeville</surname><given-names>G</given-names></name><name><surname>Tauzin</surname><given-names>Y</given-names></name><name><surname>Laigret</surname><given-names>F</given-names></name><name><surname>Moing</surname><given-names>A</given-names></name><article-title>Development of a second generation genetic linkage map for peach [<italic>Prunus persica </italic>(L.) Batsch] and characterization of morphological traits affecting flower and fruit</article-title><source>Tree Genet Genomes</source><year>2006</year><volume>3</volume><fpage>1</fpage><lpage>13</lpage><pub-id pub-id-type="doi">10.1007/s11295-006-0053-1</pub-id></mixed-citation></ref><ref id="B30"><mixed-citation publication-type="journal"><name><surname>Bailey</surname><given-names>JS</given-names></name><name><surname>French</surname><given-names>AP</given-names></name><article-title>The inheritance of blossom type and blossom size in peach</article-title><source>Proc Am Soc Hort Sci</source><year>1942</year><volume>40</volume><fpage>248</fpage><lpage>250</lpage></mixed-citation></ref><ref id="B31"><mixed-citation publication-type="journal"><name><surname>Peace</surname><given-names>CP</given-names></name><name><surname>Ahmad</surname><given-names>R</given-names></name><name><surname>Gradziel</surname><given-names>TM</given-names></name><name><surname>Dandekar</surname><given-names>AM</given-names></name><name><surname>Crisosto</surname><given-names>CH</given-names></name><article-title>The use of molecular genetics to improve peach and nectarine post-storage quality</article-title><source>Acta Hort</source><year>2005</year><volume>682</volume><fpage>403</fpage><lpage>409</lpage></mixed-citation></ref><ref id="B32"><mixed-citation publication-type="journal"><name><surname>Peace</surname><given-names>CP</given-names></name><name><surname>Crisosto</surname><given-names>CH</given-names></name><name><surname>Gradziel</surname><given-names>TM</given-names></name><article-title>Endopolygalacturonase: a candidate gene for <italic>Freestone </italic>and <italic>Melting flesh </italic>in peach</article-title><source>Mol Breed</source><year>2005</year><volume>16</volume><fpage>21</fpage><lpage>31</lpage><pub-id pub-id-type="doi">10.1007/s11032-005-0828-3</pub-id></mixed-citation></ref><ref id="B33"><mixed-citation publication-type="journal"><name><surname>Ogundiwin</surname><given-names>EA</given-names></name><name><surname>Peace</surname><given-names>CP</given-names></name><name><surname>Nicolet</surname><given-names>CM</given-names></name><name><surname>Rashbrook</surname><given-names>VK</given-names></name><name><surname>Gradziel</surname><given-names>TM</given-names></name><name><surname>Bliss</surname><given-names>FA</given-names></name><name><surname>Parfitt</surname><given-names>D</given-names></name><name><surname>Crisosto</surname><given-names>CH</given-names></name><article-title>Leucoanthocyanidin dioxygenase gene (PpLDOX): a potential functional marker for cold storage browning in peach</article-title><source>Tree Genet Genomes</source><year>2008</year><volume>4</volume><fpage>543</fpage><lpage>554</lpage><pub-id pub-id-type="doi">10.1007/s11295-007-0130-0</pub-id></mixed-citation></ref><ref id="B34"><mixed-citation publication-type="journal"><name><surname>Quilot</surname><given-names>B</given-names></name><name><surname>Wu</surname><given-names>BH</given-names></name><name><surname>Kervella</surname><given-names>J</given-names></name><name><surname>Genard</surname><given-names>M</given-names></name><name><surname>Foulongne</surname><given-names>M</given-names></name><name><surname>Moreau</surname><given-names>K</given-names></name><article-title>QTL analysis of quality traits in an advanced backcross between <italic>Prunus persica </italic>cultivars and the wild related species <italic>P</italic>. <italic>davidiana</italic></article-title><source>Theor Appl Genet</source><year>2004</year><volume>109</volume><fpage>884</fpage><lpage>897</lpage><pub-id pub-id-type="doi">10.1007/s00122-004-1703-z</pub-id><pub-id pub-id-type="pmid">15168024</pub-id></mixed-citation></ref><ref id="B35"><mixed-citation publication-type="journal"><name><surname>Ogundiwin</surname><given-names>EA</given-names></name><name><surname>Martí</surname><given-names>C</given-names></name><name><surname>Forment</surname><given-names>J</given-names></name><name><surname>Pons</surname><given-names>C</given-names></name><name><surname>Granell</surname><given-names>A</given-names></name><name><surname>Gradziel</surname><given-names>TM</given-names></name><name><surname>Peace</surname><given-names>CP</given-names></name><name><surname>Crisosto</surname><given-names>CH</given-names></name><article-title>Development of ChillPeach genomic tools and identification of cold-responsive genes in peach fruit</article-title><source>Plant Mol Biol</source><year>2008</year><volume>68</volume><fpage>379</fpage><lpage>397</lpage><pub-id pub-id-type="doi">10.1007/s11103-008-9378-5</pub-id><pub-id pub-id-type="pmid">18661259</pub-id></mixed-citation></ref><ref id="B36"><mixed-citation publication-type="journal"><name><surname>Monet</surname><given-names>R</given-names></name><name><surname>Guye</surname><given-names>A</given-names></name><name><surname>Roy</surname><given-names>M</given-names></name><name><surname>Dachary</surname><given-names>N</given-names></name><article-title>Peach Mendelian genetics a short review and new results</article-title><source>Agronomie</source><year>1996</year><volume>16</volume><fpage>321</fpage><lpage>329</lpage><pub-id pub-id-type="doi">10.1051/agro:19960505</pub-id></mixed-citation></ref><ref id="B37"><mixed-citation publication-type="journal"><name><surname>Etienne</surname><given-names>C</given-names></name><name><surname>Moing</surname><given-names>A</given-names></name><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Raymond</surname><given-names>P</given-names></name><name><surname>Monet</surname><given-names>R</given-names></name><name><surname>Rothan</surname><given-names>C</given-names></name><article-title>Isolation and characterization of six peach cDNAs encoding key proteins in organic acid metabolism and solute accumulation: involvement in regulating peach fruit acidity</article-title><source>Physiol Plantarum</source><year>2002</year><volume>114</volume><fpage>259</fpage><lpage>270</lpage><pub-id pub-id-type="doi">10.1034/j.1399-3054.2002.1140212.x</pub-id></mixed-citation></ref><ref id="B38"><mixed-citation publication-type="journal"><name><surname>Peace</surname><given-names>CP</given-names></name><name><surname>Crisosto</surname><given-names>CH</given-names></name><name><surname>Garner</surname><given-names>DT</given-names></name><name><surname>Dandekar</surname><given-names>AM</given-names></name><name><surname>Gradziel</surname><given-names>TM</given-names></name><name><surname>Bliss</surname><given-names>FA</given-names></name><article-title>Genetic control of internal breakdown in peach</article-title><source>Acta Hort</source><year>2006</year><volume>713</volume><fpage>489</fpage><lpage>496</lpage></mixed-citation></ref><ref id="B39"><mixed-citation publication-type="journal"><name><surname>Ogundiwin</surname><given-names>EA</given-names></name><name><surname>Peace</surname><given-names>CP</given-names></name><name><surname>Gradziel</surname><given-names>TM</given-names></name><name><surname>Dandekar</surname><given-names>AM</given-names></name><name><surname>Bliss</surname><given-names>FA</given-names></name><name><surname>Crisosto</surname><given-names>CH</given-names></name><article-title>Molecular genetic dissection of chilling injury in peach fruit</article-title><source>Acta Hort</source><year>2007</year><volume>738</volume><fpage>633</fpage><lpage>638</lpage></mixed-citation></ref><ref id="B40"><mixed-citation publication-type="journal"><name><surname>Lopukhina</surname><given-names>A</given-names></name><name><surname>Dettenberg</surname><given-names>M</given-names></name><name><surname>Weiler</surname><given-names>EW</given-names></name><name><surname>Hollander-Czytko</surname><given-names>H</given-names></name><article-title>Cloning and characterization of a coronatine-regulated tyrosine aminotransferase from <italic>Arabidopsis</italic></article-title><source>Plant Physiol</source><year>2001</year><volume>126</volume><fpage>1678</fpage><lpage>1687</lpage><pub-id pub-id-type="doi">10.1104/pp.126.4.1678</pub-id><pub-id pub-id-type="pmid">11500565</pub-id></mixed-citation></ref><ref id="B41"><mixed-citation publication-type="journal"><name><surname>Trainotti</surname><given-names>L</given-names></name><name><surname>Zanin</surname><given-names>D</given-names></name><name><surname>Casadoro</surname><given-names>G</given-names></name><article-title>A cell wall-oriented genomic approach reveals a new and unexpected complexity of the softening in peaches</article-title><source>J Exp Bot</source><year>2003</year><volume>54</volume><fpage>1821</fpage><lpage>1832</lpage><pub-id pub-id-type="doi">10.1093/jxb/erg198</pub-id><pub-id pub-id-type="pmid">12815031</pub-id></mixed-citation></ref><ref id="B42"><mixed-citation publication-type="journal"><name><surname>Bouvier</surname><given-names>F</given-names></name><name><surname>D'Harlingue</surname><given-names>A</given-names></name><name><surname>Backhaus</surname><given-names>RA</given-names></name><name><surname>Kumagai</surname><given-names>MH</given-names></name><name><surname>Camara</surname><given-names>B</given-names></name><article-title>Identification of neoxanthin synthase as a carotenoid cyclase paralog</article-title><source>Eur J Biochem</source><year>2001</year><volume>267</volume><fpage>6346</fpage><lpage>6352</lpage><pub-id pub-id-type="doi">10.1046/j.1432-1327.2000.01722.x</pub-id></mixed-citation></ref><ref id="B43"><mixed-citation publication-type="journal"><name><surname>Marin-Rodriguez</surname><given-names>MC</given-names></name><name><surname>Orchard</surname><given-names>J</given-names></name><name><surname>Seymour</surname><given-names>GB</given-names></name><article-title>Pectate lyases, cell wall degradation and fruit softening</article-title><source>J Exp Bot</source><year>2002</year><volume>53</volume><fpage>2115</fpage><lpage>2119</lpage><pub-id pub-id-type="doi">10.1093/jxb/erf089</pub-id><pub-id pub-id-type="pmid">12324535</pub-id></mixed-citation></ref><ref id="B44"><mixed-citation publication-type="journal"><name><surname>Tipping</surname><given-names>AJ</given-names></name><name><surname>McPherson</surname><given-names>MJ</given-names></name><article-title>Cloning and molecular analysis of the pea seedling copper amine oxidase</article-title><source>J Biol Chem</source><year>1995</year><volume>270</volume><fpage>16939</fpage><lpage>16946</lpage><pub-id pub-id-type="doi">10.1074/jbc.270.28.16939</pub-id><pub-id pub-id-type="pmid">7622512</pub-id></mixed-citation></ref><ref id="B45"><mixed-citation publication-type="journal"><name><surname>Luan</surname><given-names>S</given-names></name><article-title>The CBL CIPK network in plant calcium signaling</article-title><source>Trends Plant Sci</source><year>2008</year><volume>14</volume><fpage>37</fpage><lpage>42</lpage><pub-id pub-id-type="doi">10.1016/j.tplants.2008.10.005</pub-id><pub-id pub-id-type="pmid">19054707</pub-id></mixed-citation></ref><ref id="B46"><mixed-citation publication-type="journal"><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Moing</surname><given-names>A</given-names></name><name><surname>Rothan</surname><given-names>C</given-names></name><name><surname>Svanella</surname><given-names>L</given-names></name><name><surname>Pronier</surname><given-names>V</given-names></name><name><surname>Guye</surname><given-names>A</given-names></name><name><surname>Plomion</surname><given-names>C</given-names></name><name><surname>Monet</surname><given-names>R</given-names></name><article-title>Mapping QTLs controlling fruit quality in peach (<italic>Prunus persica </italic>(L.) Batsch)</article-title><source>Theor Appl Genet</source><year>1999</year><volume>98</volume><fpage>18</fpage><lpage>31</lpage><pub-id pub-id-type="doi">10.1007/s001220051035</pub-id></mixed-citation></ref><ref id="B47"><mixed-citation publication-type="journal"><name><surname>Miclet</surname><given-names>E</given-names></name><name><surname>Stoven</surname><given-names>V</given-names></name><name><surname>Michels</surname><given-names>PAM</given-names></name><name><surname>Opperdoes</surname><given-names>FR</given-names></name><name><surname>Lallemand</surname><given-names>J-V</given-names></name><name><surname>Duffieux</surname><given-names>F</given-names></name><article-title>NMR spectroscopic analysis of the first two steps of the pentose-phosphate pathway elucidates the role of 6-phosphogluconolactonase</article-title><source>J Biol Chem</source><year>2001</year><volume>276</volume><fpage>34840</fpage><lpage>34846</lpage><pub-id pub-id-type="doi">10.1074/jbc.M105174200</pub-id><pub-id pub-id-type="pmid">11457850</pub-id></mixed-citation></ref><ref id="B48"><mixed-citation publication-type="journal"><name><surname>Ishiguro</surname><given-names>S</given-names></name><name><surname>Nakamura</surname><given-names>K</given-names></name><article-title>Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the 5' upstream regions of genes coding for sporamin and betaamylase from sweet potato</article-title><source>Mol Gen Genet</source><year>1994</year><volume>244</volume><fpage>563</fpage><lpage>571</lpage><pub-id pub-id-type="doi">10.1007/BF00282746</pub-id><pub-id pub-id-type="pmid">7969025</pub-id></mixed-citation></ref><ref id="B49"><mixed-citation publication-type="journal"><name><surname>Downs</surname><given-names>CG</given-names></name><name><surname>Somerfield</surname><given-names>SD</given-names></name><article-title>Asparagine synthetase gene expression increases as sucrose declines in broccoli after harvest</article-title><source>NZ J Crop Hort Sci</source><year>1997</year><volume>25</volume><fpage>191</fpage><lpage>195</lpage><comment>full_text</comment></mixed-citation></ref><ref id="B50"><mixed-citation publication-type="journal"><name><surname>Goldsmith-Fischman</surname><given-names>S</given-names></name><name><surname>Kuzin</surname><given-names>A</given-names></name><name><surname>Edstrom</surname><given-names>WC</given-names></name><name><surname>Benach</surname><given-names>J</given-names></name><name><surname>Shastry</surname><given-names>R</given-names></name><name><surname>Xiao</surname><given-names>R</given-names></name><name><surname>Acton</surname><given-names>TB</given-names></name><name><surname>Honig</surname><given-names>B</given-names></name><name><surname>Montelione</surname><given-names>GT</given-names></name><name><surname>Hunt</surname><given-names>JF</given-names></name><article-title>The SufE sulfur-acceptor protein contains a conserved core structure that mediates interdomain interactions in a variety of redox protein complexes</article-title><source>J Mol Biol</source><year>2004</year><volume>344</volume><fpage>549</fpage><lpage>565</lpage><pub-id pub-id-type="doi">10.1016/j.jmb.2004.08.074</pub-id><pub-id pub-id-type="pmid">15522304</pub-id></mixed-citation></ref><ref id="B51"><mixed-citation publication-type="journal"><name><surname>Milewski</surname><given-names>S</given-names></name><article-title>Glucosamine-6-phosphate synthase - the multi-facets enzyme</article-title><source>Biochim Biophys Acta Prot Struct Mol Enzymol</source><year>2002</year><volume>1597</volume><fpage>173</fpage><lpage>192</lpage><pub-id pub-id-type="doi">10.1016/S0167-4838(02)00318-7</pub-id></mixed-citation></ref><ref id="B52"><mixed-citation publication-type="journal"><name><surname>Kader</surname><given-names>AA</given-names></name><name><surname>Chordas</surname><given-names>A</given-names></name><article-title>Evaluating the browning potential of peaches</article-title><source>Calif Agr</source><year>1984</year><volume>38</volume><fpage>14</fpage><lpage>15</lpage></mixed-citation></ref><ref id="B53"><mixed-citation publication-type="journal"><name><surname>O'Neill</surname><given-names>SD</given-names></name><name><surname>Tong</surname><given-names>Y</given-names></name><name><surname>Sporlein</surname><given-names>B</given-names></name><name><surname>Forkmann</surname><given-names>G</given-names></name><name><surname>Yoder</surname><given-names>JI</given-names></name><article-title>Molecular genetic analysis of chalcone synthase in <italic>Lycopersicon esculentum </italic>and an anthocyanin-deficient mutant</article-title><source>Mol Gen Genet</source><year>1990</year><volume>224</volume><fpage>279</fpage><lpage>288</lpage><pub-id pub-id-type="doi">10.1007/BF00271562</pub-id><pub-id pub-id-type="pmid">1980524</pub-id></mixed-citation></ref><ref id="B54"><mixed-citation publication-type="journal"><name><surname>Bonghi</surname><given-names>C</given-names></name><name><surname>Rascio</surname><given-names>N</given-names></name><name><surname>Ramina</surname><given-names>A</given-names></name><name><surname>Casadoro</surname><given-names>G</given-names></name><article-title>Cellulase and polygalacturonase involvement in the abscission of leaf and fruit explants of peach</article-title><source>Plant Mol Biol</source><year>1992</year><volume>20</volume><fpage>839</fpage><lpage>848</lpage><pub-id pub-id-type="doi">10.1007/BF00027155</pub-id><pub-id pub-id-type="pmid">1281437</pub-id></mixed-citation></ref><ref id="B55"><mixed-citation publication-type="journal"><name><surname>Martin</surname><given-names>T</given-names></name><name><surname>Frommer</surname><given-names>WB</given-names></name><name><surname>Salanoubat</surname><given-names>M</given-names></name><name><surname>Willmitzer</surname><given-names>L</given-names></name><article-title>Expression of an Arabidopsis sucrose synthase gene indicates a role in metabolization of sucrose both during phloem loading and in sink organs</article-title><source>Plant J</source><year>1993</year><volume>4</volume><fpage>367</fpage><lpage>377</lpage><pub-id pub-id-type="doi">10.1046/j.1365-313X.1993.04020367.x</pub-id><pub-id pub-id-type="pmid">8220487</pub-id></mixed-citation></ref><ref id="B56"><mixed-citation publication-type="journal"><name><surname>Cunningham</surname><given-names>FX</given-names></name><name><surname>Sun</surname><given-names>Z</given-names></name><name><surname>Chamovitz</surname><given-names>D</given-names></name><name><surname>Hirschberg</surname><given-names>J</given-names></name><name><surname>Grantt</surname><given-names>E</given-names></name><article-title>Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation</article-title><source>Plant Cell</source><year>1996</year><volume>8</volume><fpage>1613</fpage><lpage>1626</lpage><pub-id pub-id-type="doi">10.1105/tpc.8.9.1613</pub-id><pub-id pub-id-type="pmid">8837512</pub-id></mixed-citation></ref><ref id="B57"><mixed-citation publication-type="journal"><name><surname>Kalaitzis</surname><given-names>P</given-names></name><name><surname>Solomos</surname><given-names>T</given-names></name><name><surname>Tucket</surname><given-names>ML</given-names></name><article-title>Three different polygalacturonases are expressed in tomato leaf and flower abscission, each with a different temporal expression pattern</article-title><source>Plant Physiol</source><year>1997</year><volume>113</volume><fpage>1303</fpage><lpage>1308</lpage><pub-id pub-id-type="doi">10.1104/pp.113.4.1303</pub-id><pub-id pub-id-type="pmid">9112778</pub-id></mixed-citation></ref><ref id="B58"><mixed-citation publication-type="journal"><name><surname>Hong</surname><given-names>SB</given-names></name><name><surname>Tucker</surname><given-names>ML</given-names></name><article-title>Genomic organization of six tomato polygalacturonases and 5' upstream sequence identity with <italic>tap1 </italic>and <italic>win2 </italic>genes</article-title><source>Mol Gen Genet</source><year>1998</year><volume>258</volume><fpage>479</fpage><lpage>487</lpage><pub-id pub-id-type="doi">10.1007/s004380050758</pub-id><pub-id pub-id-type="pmid">9669329</pub-id></mixed-citation></ref><ref id="B59"><mixed-citation publication-type="journal"><name><surname>Chang</surname><given-names>S</given-names></name><name><surname>Tan</surname><given-names>C</given-names></name><name><surname>Frankel</surname><given-names>EN</given-names></name><name><surname>Barrett</surname><given-names>DM</given-names></name><article-title>Low-density lipoprotein antioxidant activity of phenolic compounds and polyphenol oxidase activity in selected clingstone peach cultivars</article-title><source>J Agric Food Chem</source><year>2000</year><volume>48</volume><fpage>147</fpage><lpage>151</lpage><pub-id pub-id-type="doi">10.1021/jf9904564</pub-id><pub-id pub-id-type="pmid">10691607</pub-id></mixed-citation></ref><ref id="B60"><mixed-citation publication-type="journal"><name><surname>Ruperti</surname><given-names>B</given-names></name><name><surname>Bonghi</surname><given-names>C</given-names></name><name><surname>Rasori</surname><given-names>A</given-names></name><name><surname>Ramina</surname><given-names>A</given-names></name><name><surname>Tonutti</surname><given-names>P</given-names></name><article-title>Characterization and expression of two members of the peach 1-aminocyclopropane-1-carboxylate oxidase gene family</article-title><source>Physiol Plantarum</source><year>2001</year><volume>111</volume><fpage>336</fpage><lpage>344</lpage><pub-id pub-id-type="doi">10.1034/j.1399-3054.2001.1110311.x</pub-id></mixed-citation></ref><ref id="B61"><mixed-citation publication-type="journal"><name><surname>Yamada</surname><given-names>K</given-names></name><name><surname>Niwa</surname><given-names>N</given-names></name><name><surname>Shiratake</surname><given-names>K</given-names></name><name><surname>Yamaki</surname><given-names>S</given-names></name><article-title>cDNA cloning of NAD-dependent sorbitol dehydrogenase from peach fruit and its expression during fruit development</article-title><source>J Hort Sci Biotechnol</source><year>2001</year><volume>76</volume><fpage>581</fpage><lpage>587</lpage></mixed-citation></ref><ref id="B62"><mixed-citation publication-type="journal"><name><surname>Jaakola</surname><given-names>L</given-names></name><name><surname>Maatta</surname><given-names>K</given-names></name><name><surname>Pirttila</surname><given-names>AN</given-names></name><name><surname>Torronen</surname><given-names>R</given-names></name><name><surname>Karenlampi</surname><given-names>S</given-names></name><name><surname>Hohtola</surname><given-names>A</given-names></name><article-title>Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin, proanthocyanindin, and flavonol levels during bilberry fruit development</article-title><source>Plant Physiol</source><year>2002</year><volume>130</volume><fpage>729</fpage><lpage>739</lpage><pub-id pub-id-type="doi">10.1104/pp.006957</pub-id><pub-id pub-id-type="pmid">12376640</pub-id></mixed-citation></ref><ref id="B63"><mixed-citation publication-type="journal"><name><surname>Fridman</surname><given-names>E</given-names></name><name><surname>Zamir</surname><given-names>D</given-names></name><article-title>Functional divergence of a syntenic invertase gene family in tomato, potato, and <italic>Arabidopsis</italic></article-title><source>Plant Physiol</source><year>2003</year><volume>131</volume><fpage>603</fpage><lpage>609</lpage><pub-id pub-id-type="doi">10.1104/pp.014431</pub-id><pub-id pub-id-type="pmid">12586884</pub-id></mixed-citation></ref><ref id="B64"><mixed-citation publication-type="journal"><name><surname>Iyidogan</surname><given-names>NF</given-names></name><name><surname>Bayindirli</surname><given-names>A</given-names></name><article-title>Effect of l-cysteine, kojic acid and 4-hexylresorcinol combination on inhibition of enzymatic browning in Amasya apple juice</article-title><source>J Food Eng</source><year>2003</year><volume>62</volume><fpage>299</fpage><lpage>304</lpage><pub-id pub-id-type="doi">10.1016/S0260-8774(03)00243-7</pub-id></mixed-citation></ref><ref id="B65"><mixed-citation publication-type="journal"><name><surname>Li</surname><given-names>Y</given-names></name><name><surname>Jones</surname><given-names>L</given-names></name><name><surname>McQueen-Mason</surname><given-names>S</given-names></name><article-title>Expansins and cell growth</article-title><source>Curr Opin Plant Biol</source><year>2003</year><volume>6</volume><fpage>603</fpage><lpage>610</lpage><pub-id pub-id-type="doi">10.1016/j.pbi.2003.09.003</pub-id><pub-id pub-id-type="pmid">14611960</pub-id></mixed-citation></ref><ref id="B66"><mixed-citation publication-type="journal"><name><surname>Trainotti</surname><given-names>L</given-names></name><name><surname>Pavanello</surname><given-names>A</given-names></name><name><surname>Zanin</surname><given-names>D</given-names></name><article-title>PpEG4 is a peach endo-beta-1,4-glucanase gene whose expression in climacteric peaches does not follow a climacteric pattern</article-title><source>J Exp Bot</source><year>2006</year><volume>57</volume><fpage>589</fpage><lpage>598</lpage><pub-id pub-id-type="doi">10.1093/jxb/erj043</pub-id><pub-id pub-id-type="pmid">16410260</pub-id></mixed-citation></ref><ref id="B67"><mixed-citation publication-type="journal"><name><surname>Bagnoli</surname><given-names>F</given-names></name><name><surname>Danti</surname><given-names>S</given-names></name><name><surname>Magherini</surname><given-names>V</given-names></name><name><surname>Cozza</surname><given-names>R</given-names></name><name><surname>Innocenti</surname><given-names>AM</given-names></name><name><surname>Racchi</surname><given-names>ML</given-names></name><article-title>Molecular cloning, characterization and expression of two catalase genes from peach</article-title><source>Funct Plant Biol</source><year>2004</year><volume>31</volume><fpage>349</fpage><lpage>357</lpage><pub-id pub-id-type="doi">10.1071/FP03203</pub-id></mixed-citation></ref><ref id="B68"><mixed-citation publication-type="journal"><name><surname>Wu</surname><given-names>Z</given-names></name><name><surname>Burns</surname><given-names>JK</given-names></name><article-title>A beta-galactosidase gene is expressed during mature fruit abscission of 'Valencia' orange (<italic>Citrus sinensis</italic>)</article-title><source>J Exp Bot</source><year>2004</year><volume>55</volume><fpage>1483</fpage><lpage>1490</lpage><pub-id pub-id-type="doi">10.1093/jxb/erh163</pub-id><pub-id pub-id-type="pmid">15208347</pub-id></mixed-citation></ref><ref id="B69"><mixed-citation publication-type="journal"><name><surname>Dal Cin</surname><given-names>V</given-names></name><name><surname>Danesin</surname><given-names>M</given-names></name><name><surname>Boschetti</surname><given-names>A</given-names></name><name><surname>Dorigoni</surname><given-names>A</given-names></name><name><surname>Ramina</surname><given-names>A</given-names></name><article-title>Ethylene biosynthesis and perception in apple fruitlet abscision (<italic>Malus domestica </italic>L. Borck)</article-title><source>J Exp Bot</source><year>2005</year><volume>56</volume><fpage>2995</fpage><lpage>3005</lpage><pub-id pub-id-type="doi">10.1093/jxb/eri296</pub-id><pub-id pub-id-type="pmid">16203755</pub-id></mixed-citation></ref><ref id="B70"><mixed-citation publication-type="journal"><name><surname>Chaves</surname><given-names>ALS</given-names></name><name><surname>de Mello-Farias</surname><given-names>PC</given-names></name><article-title>Ethylene and fruit ripening: From illumination gas to the control of gene expression, more than a century of discoveries</article-title><source>Genet Mol Biol</source><year>2006</year><volume>29</volume><fpage>508</fpage><lpage>515</lpage></mixed-citation></ref><ref id="B71"><mixed-citation publication-type="journal"><name><surname>Tieman</surname><given-names>D</given-names></name><name><surname>Taylor</surname><given-names>M</given-names></name><name><surname>Schauer</surname><given-names>N</given-names></name><name><surname>Fernie</surname><given-names>AR</given-names></name><name><surname>Hanson</surname><given-names>AD</given-names></name><name><surname>Klee</surname><given-names>HJ</given-names></name><article-title>Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde</article-title><source>Proc Natl Acad Sci USA</source><year>2006</year><volume>103</volume><fpage>8287</fpage><lpage>8292</lpage><pub-id pub-id-type="doi">10.1073/pnas.0602469103</pub-id><pub-id pub-id-type="pmid">16698923</pub-id></mixed-citation></ref><ref id="B72"><mixed-citation publication-type="journal"><name><surname>Dirlewanger</surname><given-names>E</given-names></name><name><surname>Cosson</surname><given-names>P</given-names></name><name><surname>Tavaud</surname><given-names>M</given-names></name><name><surname>Aranzana</surname><given-names>MJ</given-names></name><name><surname>Poizat</surname><given-names>C</given-names></name><name><surname>Zanetto</surname><given-names>A</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><name><surname>Laigret</surname><given-names>R</given-names></name><article-title>Development of microsatellite markers in peach (<italic>Prunus persica </italic>(L.) Batsch) and their use in genetic diversity analysis in peach and sweet cherry (<italic>Prunus avium </italic>L.)</article-title><source>Theor Appl Genet</source><year>2002</year><volume>105</volume><fpage>127</fpage><lpage>138</lpage><pub-id pub-id-type="doi">10.1007/s00122-002-0867-7</pub-id><pub-id pub-id-type="pmid">12582570</pub-id></mixed-citation></ref><ref id="B73"><mixed-citation publication-type="journal"><name><surname>Aranzana</surname><given-names>MJ</given-names></name><name><surname>Garcia-Mas</surname><given-names>J</given-names></name><name><surname>Carbo</surname><given-names>J</given-names></name><name><surname>Arús</surname><given-names>P</given-names></name><article-title>Development and variability analysis of microsatellite markers in peach</article-title><source>Plant Breed</source><year>2002</year><volume>121</volume><fpage>87</fpage><lpage>92</lpage><pub-id pub-id-type="doi">10.1046/j.1439-0523.2002.00656.x</pub-id></mixed-citation></ref><ref id="B74"><mixed-citation publication-type="journal"><name><surname>Sosinski</surname><given-names>B</given-names></name><name><surname>Gannavarapu</surname><given-names>M</given-names></name><name><surname>Hager</surname><given-names>LD</given-names></name><name><surname>Beck</surname><given-names>LE</given-names></name><name><surname>King</surname><given-names>GJ</given-names></name><name><surname>Ryder</surname><given-names>CD</given-names></name><name><surname>Rajapakse</surname><given-names>S</given-names></name><name><surname>Baird</surname><given-names>WV</given-names></name><name><surname>Ballard</surname><given-names>RE</given-names></name><name><surname>Abbott</surname><given-names>AG</given-names></name><article-title>Characterization of microsatellite markers in peach (<italic>Prunus persica </italic>(L.) Batsch)</article-title><source>Theor Appl Genet</source><year>2000</year><volume>101</volume><fpage>421</fpage><lpage>428</lpage><pub-id pub-id-type="doi">10.1007/s001220051499</pub-id></mixed-citation></ref><ref id="B75"><mixed-citation publication-type="journal"><name><surname>Cantini</surname><given-names>C</given-names></name><name><surname>Iezzoni</surname><given-names>AF</given-names></name><name><surname>Lamboy</surname><given-names>WF</given-names></name><name><surname>Boritzki</surname><given-names>M</given-names></name><name><surname>Struss</surname><given-names>D</given-names></name><article-title>DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats</article-title><source>J Am Soc Hort Sci</source><year>2001</year><volume>126</volume><fpage>205</fpage><lpage>209</lpage></mixed-citation></ref><ref id="B76"><mixed-citation publication-type="journal"><name><surname>Struss</surname><given-names>D</given-names></name><name><surname>Ahmad</surname><given-names>R</given-names></name><name><surname>Southwick</surname><given-names>SM</given-names></name><name><surname>Boritzki</surname><given-names>M</given-names></name><article-title>Analysis of sweet cherry (<italic>Prunus avium </italic>L.) cultivars using SSR and AFLP markers</article-title><source>J Am Soc Hort Sci</source><year>2003</year><volume>128</volume><fpage>904</fpage><lpage>909</lpage></mixed-citation></ref><ref id="B77"><mixed-citation publication-type="journal"><name><surname>Cipriani</surname><given-names>G</given-names></name><name><surname>Lot</surname><given-names>G</given-names></name><name><surname>Huang</surname><given-names>WG</given-names></name><name><surname>Marrazzo</surname><given-names>MT</given-names></name><name><surname>Peterlunger</surname><given-names>E</given-names></name><name><surname>Testolin</surname><given-names>R</given-names></name><article-title>AC/GT and AG/CT microsatellite repeats in peach (Prunus persica (L.) Batsch): isolation, characterization and cross-species amplification in <italic>Prunus</italic></article-title><source>Theor Appl Genet</source><year>1999</year><volume>99</volume><fpage>65</fpage><lpage>72</lpage><pub-id pub-id-type="doi">10.1007/s001220051209</pub-id></mixed-citation></ref><ref id="B78"><mixed-citation publication-type="journal"><name><surname>Testolin</surname><given-names>R</given-names></name><name><surname>Marrazzo</surname><given-names>T</given-names></name><name><surname>Cipriani</surname><given-names>G</given-names></name><name><surname>Quarta</surname><given-names>R</given-names></name><name><surname>Verde</surname><given-names>I</given-names></name><name><surname>Dettori</surname><given-names>MT</given-names></name><name><surname>Pancaldi</surname><given-names>M</given-names></name><name><surname>Sansavini</surname><given-names>S</given-names></name><article-title>Microsatellite DNA in peach (<italic>Prunus persica </italic>L. Batch) and its use in fingerprinting and testing the genetic origin of cultivars</article-title><source>Genome</source><year>2000</year><volume>43</volume><fpage>512</fpage><lpage>520</lpage><pub-id pub-id-type="doi">10.1139/gen-43-3-512</pub-id><pub-id pub-id-type="pmid">10902716</pub-id></mixed-citation></ref><ref id="B79"><mixed-citation publication-type="journal"><name><surname>Testolin</surname><given-names>R</given-names></name><name><surname>Messina</surname><given-names>R</given-names></name><name><surname>Lain</surname><given-names>O</given-names></name><name><surname>Marrazzo</surname><given-names>MT</given-names></name><name><surname>Huang</surname><given-names>WG</given-names></name><name><surname>Cipriani</surname><given-names>G</given-names></name><article-title>Microsatellites isolated in almond from an AC-repeat enriched library</article-title><source>Mol Ecol Notes</source><year>2004</year><volume>4</volume><fpage>459</fpage><lpage>461</lpage><pub-id pub-id-type="doi">10.1111/j.1471-8286.2004.00700.x</pub-id></mixed-citation></ref><ref id="B80"><mixed-citation publication-type="journal"><name><surname>Waldron</surname><given-names>J</given-names></name><name><surname>Peace</surname><given-names>CP</given-names></name><name><surname>Searle</surname><given-names>IR</given-names></name><name><surname>Furtado</surname><given-names>A</given-names></name><name><surname>Wade</surname><given-names>N</given-names></name><name><surname>Graham</surname><given-names>MW</given-names></name><name><surname>Carroll</surname><given-names>BJ</given-names></name><article-title>Randomly Amplified DNA Fingerprinting: A culmination of DNA marker technologies based on arbitrarily-primed PCR amplification</article-title><source>J Biomed Biotechnol</source><year>2002</year><volume>2</volume><fpage>141</fpage><lpage>150</lpage><pub-id pub-id-type="doi">10.1155/S1110724302206026</pub-id><pub-id pub-id-type="pmid">12488579</pub-id></mixed-citation></ref><ref id="B81"><mixed-citation publication-type="journal"><name><surname>Michelmore</surname><given-names>RW</given-names></name><name><surname>Paran</surname><given-names>I</given-names></name><name><surname>Kesseli</surname><given-names>RV</given-names></name><article-title>Identification of markers lined to diseaseresistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations</article-title><source>Proc Natl Acad Sci USA</source><year>1991</year><volume>88</volume><fpage>9828</fpage><lpage>9832</lpage><pub-id pub-id-type="doi">10.1073/pnas.88.21.9828</pub-id><pub-id pub-id-type="pmid">1682921</pub-id></mixed-citation></ref><ref id="B82"><mixed-citation publication-type="journal"><name><surname>Ahmad</surname><given-names>R</given-names></name><name><surname>Potter</surname><given-names>D</given-names></name><name><surname>Southwick</surname><given-names>SM</given-names></name><article-title>Genotyping of peach and nectarine cultivars with SSR and SRAP molecular markers</article-title><source>J Amer Soc Hort Sci</source><year>2004</year><volume>129</volume><fpage>204</fpage><lpage>210</lpage></mixed-citation></ref><ref id="B83"><mixed-citation publication-type="book"><name><surname>Rozen</surname><given-names>S</given-names></name><name><surname>Skaletsky</surname><given-names>HJ</given-names></name><person-group person-group-type="editor">Krawetz, S, Misener S</person-group><article-title>Primer3 on the WWW for general users and for biologist programmers</article-title><source>Bioinformatics Methods and Protocols: Methods in Molecular Biology</source><year>2000</year><publisher-name>Totowa: Humana Press</publisher-name><fpage>365</fpage><lpage>386</lpage></mixed-citation></ref><ref id="B84"><mixed-citation publication-type="book"><name><surname>Van Ooijen</surname><given-names>JW</given-names></name><source>JoinMap<sup>® </sup>4, Software for the calculation of genetic linkage maps in experimental populations</source><year>2006</year><publisher-name>Kyazma B.V., Wageningen, Netherlands</publisher-name></mixed-citation></ref><ref id="B85"><mixed-citation publication-type="journal"><name><surname>Kosambi</surname><given-names>DD</given-names></name><article-title>The estimation of map distance from recombination values</article-title><source>Ann Eugen</source><year>1944</year><volume>12</volume><fpage>172</fpage><lpage>175</lpage></mixed-citation></ref></ref-list></back></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E09 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000E09 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.25. |  |