Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The p53, Bax and p21 dependent inhibition of colon cancer cell growth by 5-hydroxy polymethoxyflavones</title><author><name sortKey="Qiu, Peiju" sort="Qiu, Peiju" uniqKey="Qiu P" first="Peiju" last="Qiu">Peiju Qiu</name><affiliation><nlm:aff id="A1">Marine Drug and Food Institute, Ocean University of China, Qingdao Shandong, China</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA USA</nlm:aff></affiliation></author><author><name sortKey="Guan, Huashi" sort="Guan, Huashi" uniqKey="Guan H" first="Huashi" last="Guan">Huashi Guan</name><affiliation><nlm:aff id="A1">Marine Drug and Food Institute, Ocean University of China, Qingdao Shandong, China</nlm:aff></affiliation></author><author><name sortKey="Dong, Ping" sort="Dong, Ping" uniqKey="Dong P" first="Ping" last="Dong">Ping Dong</name><affiliation><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA USA</nlm:aff></affiliation></author><author><name sortKey="Li, Shiming" sort="Li, Shiming" uniqKey="Li S" first="Shiming" last="Li">Shiming Li</name><affiliation><nlm:aff id="A3">WellGen, Inc., North Brunswick, NJ USA</nlm:aff></affiliation></author><author><name sortKey="Ho, Chi Tang" sort="Ho, Chi Tang" uniqKey="Ho C" first="Chi-Tang" last="Ho">Chi-Tang Ho</name><affiliation><nlm:aff id="A4">Department of Food Science, Rutgers, The State University of New Jersey, New Brunswick, NJ USA</nlm:aff></affiliation></author><author><name sortKey="Pan, Min Hsiung" sort="Pan, Min Hsiung" uniqKey="Pan M" first="Min-Hsiung" last="Pan">Min-Hsiung Pan</name><affiliation><nlm:aff id="A5">Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan</nlm:aff></affiliation></author><author><name sortKey="Mcclements, David Julian" sort="Mcclements, David Julian" uniqKey="Mcclements D" first="David Julian" last="Mcclements">David Julian Mcclements</name><affiliation><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA USA</nlm:aff></affiliation></author><author><name sortKey="Xiao, Hang" sort="Xiao, Hang" uniqKey="Xiao H" first="Hang" last="Xiao">Hang Xiao</name><affiliation><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21462329</idno><idno type="pmc">3139001</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3139001</idno><idno type="RBID">PMC:3139001</idno><idno type="doi">10.1002/mnfr.201000269</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000D64</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The p53, Bax and p21 dependent inhibition of colon cancer cell growth by 5-hydroxy polymethoxyflavones</title><author><name sortKey="Qiu, Peiju" sort="Qiu, Peiju" uniqKey="Qiu P" first="Peiju" last="Qiu">Peiju Qiu</name><affiliation><nlm:aff id="A1">Marine Drug and Food Institute, Ocean University of China, Qingdao Shandong, China</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA USA</nlm:aff></affiliation></author><author><name sortKey="Guan, Huashi" sort="Guan, Huashi" uniqKey="Guan H" first="Huashi" last="Guan">Huashi Guan</name><affiliation><nlm:aff id="A1">Marine Drug and Food Institute, Ocean University of China, Qingdao Shandong, China</nlm:aff></affiliation></author><author><name sortKey="Dong, Ping" sort="Dong, Ping" uniqKey="Dong P" first="Ping" last="Dong">Ping Dong</name><affiliation><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA USA</nlm:aff></affiliation></author><author><name sortKey="Li, Shiming" sort="Li, Shiming" uniqKey="Li S" first="Shiming" last="Li">Shiming Li</name><affiliation><nlm:aff id="A3">WellGen, Inc., North Brunswick, NJ USA</nlm:aff></affiliation></author><author><name sortKey="Ho, Chi Tang" sort="Ho, Chi Tang" uniqKey="Ho C" first="Chi-Tang" last="Ho">Chi-Tang Ho</name><affiliation><nlm:aff id="A4">Department of Food Science, Rutgers, The State University of New Jersey, New Brunswick, NJ USA</nlm:aff></affiliation></author><author><name sortKey="Pan, Min Hsiung" sort="Pan, Min Hsiung" uniqKey="Pan M" first="Min-Hsiung" last="Pan">Min-Hsiung Pan</name><affiliation><nlm:aff id="A5">Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan</nlm:aff></affiliation></author><author><name sortKey="Mcclements, David Julian" sort="Mcclements, David Julian" uniqKey="Mcclements D" first="David Julian" last="Mcclements">David Julian Mcclements</name><affiliation><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA USA</nlm:aff></affiliation></author><author><name sortKey="Xiao, Hang" sort="Xiao, Hang" uniqKey="Xiao H" first="Hang" last="Xiao">Hang Xiao</name><affiliation><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA USA</nlm:aff></affiliation></author></analytic><series><title level="j">Molecular nutrition & food research</title><idno type="ISSN">1613-4125</idno><idno type="eISSN">1613-4133</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Previously, we reported that 5 hydroxy polymethoxyflavones (5OH-PMFs) isolated from orange, namely 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone (5HPMF), 5-hydroxy-3,6,7,8,3′,4′-hexamethoxyflavone (5HHMF), and 5-hydroxy-6,7,8,4´-tetramethoxyflavone (5HTMF) potently induced apoptosis and cell cycle arrest in multiple human colon cancer cells. Herein, using isogenic variants of HCT116 human colon cancer cells, we investigated the effects of p53, Bax and p21 on the apoptosis and cell cycle arrest induced by different 5OH-PMFs. Annexin V/PI co-staining assay demonstrated that 5HHMF and 5HTMF significantly induced apoptosis in HCT116 (p53 +/+) cells, but not in HCT116 (p53 −/−) cells. Further more, 5HHMF and 5HTMF significantly induced apoptosis in HCT116 (Bax +/−) cells, while their pro-apoptotic effects on HCT116 (Bax −/−) cells were marginal. All three 5OH-PMFs increased G0/G1 cell population of HCT116 (p53 +/+) cells, and these effects were abolished in HCT116 (p53 −/−) and HCT116 (p21 −/−) cells. Immunoblotting analysis showed that 5HHMF and 5HTMF increased the levels of cleaved caspase-3, cleaved PARP in both HCT116 (p53 +/+) and HCT116 (Bax +/−) cells, and these effects were much weaker in HCT116 (p53 −/−) and HCT116 (Bax −/−) cells. Taken together, our results demonstrated that 5OH-PMFs, especially 5HHMF and 5HTMF induce apoptosis and cell cycle arrest by p53, Bax and p21 dependent mechanisms.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101231818</journal-id><journal-id journal-id-type="pubmed-jr-id">32150</journal-id><journal-id journal-id-type="nlm-ta">Mol Nutr Food Res</journal-id><journal-id journal-id-type="iso-abbrev">Mol Nutr Food Res</journal-id><journal-title-group><journal-title>Molecular nutrition & food research</journal-title></journal-title-group><issn pub-type="ppub">1613-4125</issn><issn pub-type="epub">1613-4133</issn></journal-meta><article-meta><article-id pub-id-type="pmid">21462329</article-id><article-id pub-id-type="pmc">3139001</article-id><article-id pub-id-type="doi">10.1002/mnfr.201000269</article-id><article-id pub-id-type="manuscript">NIHMS261429</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>The p53, Bax and p21 dependent inhibition of colon cancer cell growth by 5-hydroxy polymethoxyflavones</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Qiu</surname><given-names>Peiju</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Guan</surname><given-names>Huashi</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Dong</surname><given-names>Ping</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Shiming</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Ho</surname><given-names>Chi-Tang</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Pan</surname><given-names>Min-Hsiung</given-names></name><xref ref-type="aff" rid="A5">5</xref></contrib><contrib contrib-type="author"><name><surname>McClements</surname><given-names>David Julian</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Xiao</surname><given-names>Hang</given-names></name><xref ref-type="aff" rid="A2">2</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="A1"><label>1</label>Marine Drug and Food Institute, Ocean University of China, Qingdao Shandong, China</aff><aff id="A2"><label>2</label>Department of Food Science, University of Massachusetts, Amherst, MA USA</aff><aff id="A3"><label>3</label>WellGen, Inc., North Brunswick, NJ USA</aff><aff id="A4"><label>4</label>Department of Food Science, Rutgers, The State University of New Jersey, New Brunswick, NJ USA</aff><aff id="A5"><label>5</label>Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan</aff><author-notes><corresp id="cor1"><label>*</label><bold><italic>Corresponding Author:</italic></bold> Hang Xiao, Department of Food Science, University of Massachusetts, 100 Holdsworth Way, Amherst, MA 01003, USA, Tel: (413) 545-2281; Fax: (413) 545-1262, <email>hangxiao@foodsci.umass.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>4</day><month>4</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>23</day><month>11</month><year>2010</year></pub-date><pub-date pub-type="ppub"><month>4</month><year>2011</year></pub-date><pub-date pub-type="pmc-release"><day>01</day><month>4</month><year>2012</year></pub-date><volume>55</volume><issue>4</issue><fpage>613</fpage><lpage>622</lpage><abstract><p id="P1">Previously, we reported that 5 hydroxy polymethoxyflavones (5OH-PMFs) isolated from orange, namely 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone (5HPMF), 5-hydroxy-3,6,7,8,3′,4′-hexamethoxyflavone (5HHMF), and 5-hydroxy-6,7,8,4´-tetramethoxyflavone (5HTMF) potently induced apoptosis and cell cycle arrest in multiple human colon cancer cells. Herein, using isogenic variants of HCT116 human colon cancer cells, we investigated the effects of p53, Bax and p21 on the apoptosis and cell cycle arrest induced by different 5OH-PMFs. Annexin V/PI co-staining assay demonstrated that 5HHMF and 5HTMF significantly induced apoptosis in HCT116 (p53 +/+) cells, but not in HCT116 (p53 −/−) cells. Further more, 5HHMF and 5HTMF significantly induced apoptosis in HCT116 (Bax +/−) cells, while their pro-apoptotic effects on HCT116 (Bax −/−) cells were marginal. All three 5OH-PMFs increased G0/G1 cell population of HCT116 (p53 +/+) cells, and these effects were abolished in HCT116 (p53 −/−) and HCT116 (p21 −/−) cells. Immunoblotting analysis showed that 5HHMF and 5HTMF increased the levels of cleaved caspase-3, cleaved PARP in both HCT116 (p53 +/+) and HCT116 (Bax +/−) cells, and these effects were much weaker in HCT116 (p53 −/−) and HCT116 (Bax −/−) cells. Taken together, our results demonstrated that 5OH-PMFs, especially 5HHMF and 5HTMF induce apoptosis and cell cycle arrest by p53, Bax and p21 dependent mechanisms.</p></abstract><kwd-group><kwd>5-hydroxy polymethoxyflavones</kwd><kwd>colon cancer</kwd><kwd>p53</kwd><kwd>Bax</kwd><kwd>p21</kwd><kwd>cell cycle arrest</kwd><kwd>apoptosis</kwd></kwd-group><funding-group><award-group><funding-source country="United States">National Cancer Institute : NCI</funding-source><award-id>R21 CA139174-01A2 || CA</award-id></award-group></funding-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D64 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000D64 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.25. |  |