Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning1[OPEN]
Identifieur interne : 000D23 ( Pmc/Corpus ); précédent : 000D22; suivant : 000D24Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning1[OPEN]
Auteurs : Gaston A. Pizzio ; Julio Paez-Valencia ; Aswad S. Khadilkar ; Kamesh Regmi ; Araceli Patron-Soberano ; Shangji Zhang ; Jonathan Sanchez-Lares ; Tara Furstenau ; Jisheng Li ; Concepcion Sanchez-Gomez ; Pedro Valencia-Mayoral ; Umesh P. Yadav ; Brian G. Ayre ; Roberto A. GaxiolaSource :
- Plant Physiology [ 0032-0889 ] ; 2015.
Abstract
The type I H+-PPase is a key component of the sieve element-companion cell complexes and involved in phloem loading in Arabidopsis.
Url:
DOI: 10.1104/pp.114.254342
PubMed: 25681328
PubMed Central: 4378163
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PMC:4378163Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></title><author><name sortKey="Pizzio, Gaston A" sort="Pizzio, Gaston A" uniqKey="Pizzio G" first="Gaston A." last="Pizzio">Gaston A. Pizzio</name></author><author><name sortKey="Paez Valencia, Julio" sort="Paez Valencia, Julio" uniqKey="Paez Valencia J" first="Julio" last="Paez-Valencia">Julio Paez-Valencia</name></author><author><name sortKey="Khadilkar, Aswad S" sort="Khadilkar, Aswad S" uniqKey="Khadilkar A" first="Aswad S." last="Khadilkar">Aswad S. Khadilkar</name></author><author><name sortKey="Regmi, Kamesh" sort="Regmi, Kamesh" uniqKey="Regmi K" first="Kamesh" last="Regmi">Kamesh Regmi</name></author><author><name sortKey="Patron Soberano, Araceli" sort="Patron Soberano, Araceli" uniqKey="Patron Soberano A" first="Araceli" last="Patron-Soberano">Araceli Patron-Soberano</name></author><author><name sortKey="Zhang, Shangji" sort="Zhang, Shangji" uniqKey="Zhang S" first="Shangji" last="Zhang">Shangji Zhang</name></author><author><name sortKey="Sanchez Lares, Jonathan" sort="Sanchez Lares, Jonathan" uniqKey="Sanchez Lares J" first="Jonathan" last="Sanchez-Lares">Jonathan Sanchez-Lares</name></author><author><name sortKey="Furstenau, Tara" sort="Furstenau, Tara" uniqKey="Furstenau T" first="Tara" last="Furstenau">Tara Furstenau</name></author><author><name sortKey="Li, Jisheng" sort="Li, Jisheng" uniqKey="Li J" first="Jisheng" last="Li">Jisheng Li</name></author><author><name sortKey="Sanchez Gomez, Concepcion" sort="Sanchez Gomez, Concepcion" uniqKey="Sanchez Gomez C" first="Concepcion" last="Sanchez-Gomez">Concepcion Sanchez-Gomez</name></author><author><name sortKey="Valencia Mayoral, Pedro" sort="Valencia Mayoral, Pedro" uniqKey="Valencia Mayoral P" first="Pedro" last="Valencia-Mayoral">Pedro Valencia-Mayoral</name></author><author><name sortKey="Yadav, Umesh P" sort="Yadav, Umesh P" uniqKey="Yadav U" first="Umesh P." last="Yadav">Umesh P. Yadav</name></author><author><name sortKey="Ayre, Brian G" sort="Ayre, Brian G" uniqKey="Ayre B" first="Brian G." last="Ayre">Brian G. Ayre</name></author><author><name sortKey="Gaxiola, Roberto A" sort="Gaxiola, Roberto A" uniqKey="Gaxiola R" first="Roberto A." last="Gaxiola">Roberto A. Gaxiola</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25681328</idno><idno type="pmc">4378163</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378163</idno><idno type="RBID">PMC:4378163</idno><idno type="doi">10.1104/pp.114.254342</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000D23</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></title><author><name sortKey="Pizzio, Gaston A" sort="Pizzio, Gaston A" uniqKey="Pizzio G" first="Gaston A." last="Pizzio">Gaston A. Pizzio</name></author><author><name sortKey="Paez Valencia, Julio" sort="Paez Valencia, Julio" uniqKey="Paez Valencia J" first="Julio" last="Paez-Valencia">Julio Paez-Valencia</name></author><author><name sortKey="Khadilkar, Aswad S" sort="Khadilkar, Aswad S" uniqKey="Khadilkar A" first="Aswad S." last="Khadilkar">Aswad S. Khadilkar</name></author><author><name sortKey="Regmi, Kamesh" sort="Regmi, Kamesh" uniqKey="Regmi K" first="Kamesh" last="Regmi">Kamesh Regmi</name></author><author><name sortKey="Patron Soberano, Araceli" sort="Patron Soberano, Araceli" uniqKey="Patron Soberano A" first="Araceli" last="Patron-Soberano">Araceli Patron-Soberano</name></author><author><name sortKey="Zhang, Shangji" sort="Zhang, Shangji" uniqKey="Zhang S" first="Shangji" last="Zhang">Shangji Zhang</name></author><author><name sortKey="Sanchez Lares, Jonathan" sort="Sanchez Lares, Jonathan" uniqKey="Sanchez Lares J" first="Jonathan" last="Sanchez-Lares">Jonathan Sanchez-Lares</name></author><author><name sortKey="Furstenau, Tara" sort="Furstenau, Tara" uniqKey="Furstenau T" first="Tara" last="Furstenau">Tara Furstenau</name></author><author><name sortKey="Li, Jisheng" sort="Li, Jisheng" uniqKey="Li J" first="Jisheng" last="Li">Jisheng Li</name></author><author><name sortKey="Sanchez Gomez, Concepcion" sort="Sanchez Gomez, Concepcion" uniqKey="Sanchez Gomez C" first="Concepcion" last="Sanchez-Gomez">Concepcion Sanchez-Gomez</name></author><author><name sortKey="Valencia Mayoral, Pedro" sort="Valencia Mayoral, Pedro" uniqKey="Valencia Mayoral P" first="Pedro" last="Valencia-Mayoral">Pedro Valencia-Mayoral</name></author><author><name sortKey="Yadav, Umesh P" sort="Yadav, Umesh P" uniqKey="Yadav U" first="Umesh P." last="Yadav">Umesh P. Yadav</name></author><author><name sortKey="Ayre, Brian G" sort="Ayre, Brian G" uniqKey="Ayre B" first="Brian G." last="Ayre">Brian G. Ayre</name></author><author><name sortKey="Gaxiola, Roberto A" sort="Gaxiola, Roberto A" uniqKey="Gaxiola R" first="Roberto A." last="Gaxiola">Roberto A. Gaxiola</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The type I H<sup>+</sup>-PPase is a key component of the sieve element-companion cell complexes and involved in phloem loading in Arabidopsis.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Plant Physiol</journal-id><journal-id journal-id-type="hwp">plantphysiol</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>Plant Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25681328</article-id><article-id pub-id-type="pmc">4378163</article-id><article-id pub-id-type="publisher-id">254342</article-id><article-id pub-id-type="doi">10.1104/pp.114.254342</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject><subj-group subj-group-type="heading"><subject>Membranes, Transport, and Bioenergetics</subject></subj-group></subj-group></article-categories><title-group><article-title>Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Pizzio</surname><given-names>Gaston A.</given-names></name><xref ref-type="author-notes" rid="afn1"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Paez-Valencia</surname><given-names>Julio</given-names></name><xref ref-type="author-notes" rid="afn1"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Khadilkar</surname><given-names>Aswad S.</given-names></name><xref ref-type="author-notes" rid="afn1"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Regmi</surname><given-names>Kamesh</given-names></name></contrib><contrib contrib-type="author"><name><surname>Patron-Soberano</surname><given-names>Araceli</given-names></name></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Shangji</given-names></name></contrib><contrib contrib-type="author"><name><surname>Sanchez-Lares</surname><given-names>Jonathan</given-names></name></contrib><contrib contrib-type="author"><name><surname>Furstenau</surname><given-names>Tara</given-names></name></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Jisheng</given-names></name></contrib><contrib contrib-type="author"><name><surname>Sanchez-Gomez</surname><given-names>Concepcion</given-names></name></contrib><contrib contrib-type="author"><name><surname>Valencia-Mayoral</surname><given-names>Pedro</given-names></name></contrib><contrib contrib-type="author"><name><surname>Yadav</surname><given-names>Umesh P.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ayre</surname><given-names>Brian G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Gaxiola</surname><given-names>Roberto A.</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff id="aff1">School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);</aff><aff id="aff2">Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);</aff><aff id="aff3">Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);</aff><aff id="aff4">División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); and</aff><aff id="aff5">Departamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.)</aff></contrib-group><author-notes><fn><p><ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org/cgi/doi/10.1104/pp.114.254342">www.plantphysiol.org/cgi/doi/10.1104/pp.114.254342</ext-link></p></fn><fn id="afn1" fn-type="equal"><label>2</label><p>These authors contributed equally to the article.</p></fn><corresp id="cor1"><label>*</label>Address correspondence to <email>roberto.gaxiola@asu.edu</email>.</corresp><fn id="afn2"><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Roberto A. Gaxiola (<email>roberto.gaxiola@asu.edu</email>).</p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher
pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>4</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>13</day><month>2</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>13</day><month>2</month><year>2015</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>167</volume><issue>4</issue><fpage>1541</fpage><lpage>1553</lpage><history><date date-type="received"><day>25</day><month>11</month><year>2014</year></date><date date-type="accepted"><day>12</day><month>2</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 American Society of Plant Biologists. All Rights Reserved.</copyright-statement><copyright-year>2015</copyright-year></permissions><self-uri xlink:role="icon" xlink:href="PP_254342_ic1.gif"></self-uri><self-uri content-type="alt-lang-pdf zh" xlink:href="1541.chinese.pdf"></self-uri><abstract abstract-type="precis"><p>The type I H<sup>+</sup>-PPase is a key component of the sieve element-companion cell complexes and involved in phloem loading in Arabidopsis.</p></abstract><abstract><p>Phloem loading is a critical process in plant physiology. The potential of regulating the translocation of photoassimilates from source to sink tissues represents an opportunity to increase crop yield. Pyrophosphate homeostasis is crucial for normal phloem function in apoplasmic loaders. The involvement of Arabidopsis (<italic>Arabidopsis thaliana</italic>) type I proton-pumping pyrophosphatase (AVP1) in phloem loading was analyzed at genetic, histochemical, and physiological levels. A transcriptional <italic>AVP1</italic> promoter::GUS fusion revealed phloem activity in source leaves. Ubiquitous <italic>AVP1</italic> overexpression (<italic>35S</italic>::<italic>AVP1</italic> cassette) enhanced shoot biomass, photoassimilate production and transport, rhizosphere acidification, and expression of sugar-induced root ion transporter genes (<italic>POTASSIUM TRANSPORTER2</italic> [<italic>KUP2</italic>], <italic>NITRATE TRANSPORTER2.1</italic> [<italic>NRT2.1</italic>], <italic>NRT2</italic><italic>.4</italic>, and <italic>PHOSPHATE TRANSPORTER1.4</italic> [<italic>PHT1.4</italic>]). Phloem-specific <italic>AVP1</italic> overexpression (<italic>Commelina Yellow Mottle Virus promoter</italic> [<italic>pCOYMV</italic>]::<italic>AVP1</italic>) elicited similar phenotypes. By contrast, phloem-specific <italic>AVP1</italic> knockdown (<italic>pCoYMV</italic>::<italic><sub>RNAi</sub>AVP1</italic>) resulted in stunted seedlings in sucrose-deprived medium. We also present a promoter mutant <italic>avp1-2</italic> (SALK046492) with a 70% reduction of expression that did not show severe growth impairment. Interestingly, AVP1 protein in this mutant is prominent in the phloem. Moreover, expression of an <italic>Escherichia coli</italic>-soluble pyrophosphatase in the phloem (<italic>pCoYMV</italic>::<italic>pyrophosphatase</italic>) of <italic>avp1-2</italic> plants resulted in severe dwarf phenotype and abnormal leaf morphology. We conclude that the Proton-Pumping Pyrophosphatase AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function.</p></abstract><counts><page-count count="13"></page-count></counts><custom-meta-group><custom-meta><meta-name> DJS Export </meta-name><meta-value>v1</meta-value></custom-meta></custom-meta-group></article-meta><notes><glossary><title>Glossary</title><def-list><def-item><term id="term1">cDNA</term><def id="def1"><p>complementary DNA</p></def></def-item><def-item><term id="term2">Col-0</term><def id="def2"><p>Columbia-0</p></def></def-item><def-item><term id="term3">Ct</term><def id="def3"><p>threshold cycle</p></def></def-item><def-item><term id="term4">DTT</term><def id="def4"><p>dithiothreitol</p></def></def-item><def-item><term id="term5">MS</term><def id="def5"><p>Murashige and Skoog</p></def></def-item><def-item><term id="term6">ORF</term><def id="def6"><p>open reading frame</p></def></def-item><def-item><term id="term7">PBS</term><def id="def7"><p>phosphate-buffered saline</p></def></def-item><def-item><term id="term8">PM</term><def id="def8"><p>plasma membrane</p></def></def-item><def-item><term id="term9">PMF</term><def id="def9"><p>proton motive force</p></def></def-item><def-item><term id="term10">PP<sub>i</sub></term><def id="def10"><p>pyrophosphate</p></def></def-item><def-item><term id="term11">qRT</term><def id="def11"><p>quantitative real-time</p></def></def-item><def-item><term id="term12">SE-CC</term><def id="def12"><p>sieve element-companion cell</p></def></def-item></def-list></glossary></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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