Gibberellin 3-oxidase Gene Expression Patterns Influence Gibberellin Biosynthesis, Growth, and Development in Pea1[W][OPEN]
Identifieur interne : 000D12 ( Pmc/Corpus ); précédent : 000D11; suivant : 000D13Gibberellin 3-oxidase Gene Expression Patterns Influence Gibberellin Biosynthesis, Growth, and Development in Pea1[W][OPEN]
Auteurs : Dennis M. Reinecke ; Aruna D. Wickramarathna ; Jocelyn A. Ozga ; Leonid V. Kurepin ; Alena L. Jin ; Allen G. Good ; Richard P. PharisSource :
- Plant Physiology [ 0032-0889 ] ; 2013.
Abstract
Url:
DOI: 10.1104/pp.113.225987
PubMed: 23979969
PubMed Central: 3793069
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PMC:3793069Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>Gibberellin 3-oxidase</italic> Gene Expression Patterns Influence Gibberellin Biosynthesis, Growth, and Development in Pea<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn3"><sup>[OPEN]</sup></xref></title><author><name sortKey="Reinecke, Dennis M" sort="Reinecke, Dennis M" uniqKey="Reinecke D" first="Dennis M." last="Reinecke">Dennis M. Reinecke</name></author><author><name sortKey="Wickramarathna, Aruna D" sort="Wickramarathna, Aruna D" uniqKey="Wickramarathna A" first="Aruna D." last="Wickramarathna">Aruna D. Wickramarathna</name></author><author><name sortKey="Ozga, Jocelyn A" sort="Ozga, Jocelyn A" uniqKey="Ozga J" first="Jocelyn A." last="Ozga">Jocelyn A. Ozga</name></author><author><name sortKey="Kurepin, Leonid V" sort="Kurepin, Leonid V" uniqKey="Kurepin L" first="Leonid V." last="Kurepin">Leonid V. Kurepin</name></author><author><name sortKey="Jin, Alena L" sort="Jin, Alena L" uniqKey="Jin A" first="Alena L." last="Jin">Alena L. Jin</name></author><author><name sortKey="Good, Allen G" sort="Good, Allen G" uniqKey="Good A" first="Allen G." last="Good">Allen G. Good</name></author><author><name sortKey="Pharis, Richard P" sort="Pharis, Richard P" uniqKey="Pharis R" first="Richard P." last="Pharis">Richard P. Pharis</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23979969</idno><idno type="pmc">3793069</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793069</idno><idno type="RBID">PMC:3793069</idno><idno type="doi">10.1104/pp.113.225987</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000D12</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>Gibberellin 3-oxidase</italic> Gene Expression Patterns Influence Gibberellin Biosynthesis, Growth, and Development in Pea<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn3"><sup>[OPEN]</sup></xref></title><author><name sortKey="Reinecke, Dennis M" sort="Reinecke, Dennis M" uniqKey="Reinecke D" first="Dennis M." last="Reinecke">Dennis M. Reinecke</name></author><author><name sortKey="Wickramarathna, Aruna D" sort="Wickramarathna, Aruna D" uniqKey="Wickramarathna A" first="Aruna D." last="Wickramarathna">Aruna D. Wickramarathna</name></author><author><name sortKey="Ozga, Jocelyn A" sort="Ozga, Jocelyn A" uniqKey="Ozga J" first="Jocelyn A." last="Ozga">Jocelyn A. Ozga</name></author><author><name sortKey="Kurepin, Leonid V" sort="Kurepin, Leonid V" uniqKey="Kurepin L" first="Leonid V." last="Kurepin">Leonid V. Kurepin</name></author><author><name sortKey="Jin, Alena L" sort="Jin, Alena L" uniqKey="Jin A" first="Alena L." last="Jin">Alena L. Jin</name></author><author><name sortKey="Good, Allen G" sort="Good, Allen G" uniqKey="Good A" first="Allen G." last="Good">Allen G. Good</name></author><author><name sortKey="Pharis, Richard P" sort="Pharis, Richard P" uniqKey="Pharis R" first="Richard P." last="Pharis">Richard P. Pharis</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>GA biosynthesis and catabolism regulation at the plant tissue and organ level is important for the timing/localization of gene expression for the production of bioactive GA, and thereby plant growth</italic>.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Plant Physiol</journal-id><journal-id journal-id-type="hwp">plantphysiol</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>Plant Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23979969</article-id><article-id pub-id-type="pmc">3793069</article-id><article-id pub-id-type="publisher-id">225987</article-id><article-id pub-id-type="doi">10.1104/pp.113.225987</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biochemistry and Metabolism</subject></subj-group></article-categories><title-group><article-title><italic>Gibberellin 3-oxidase</italic> Gene Expression Patterns Influence Gibberellin Biosynthesis, Growth, and Development in Pea<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn3"><sup>[OPEN]</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Reinecke</surname><given-names>Dennis M.</given-names></name><xref ref-type="author-notes" rid="afn1"><sup>2</sup></xref><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-5650-5644</contrib-id></contrib><contrib contrib-type="author"><name><surname>Wickramarathna</surname><given-names>Aruna D.</given-names></name><xref ref-type="author-notes" rid="afn1"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ozga</surname><given-names>Jocelyn A.</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Kurepin</surname><given-names>Leonid V.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Jin</surname><given-names>Alena L.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Good</surname><given-names>Allen G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Pharis</surname><given-names>Richard P.</given-names></name></contrib><aff id="aff1">Plant BioSystems, Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5 (D.M.R., A.D.W., J.A.O., A.L.J.)</aff><aff id="aff2">Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4 (L.V.K., R.P.P.); and</aff><aff id="aff3">Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 (A.G.G.)</aff></contrib-group><author-notes><fn id="fn1" fn-type="supported-by"><label>1</label><p>This work was supported by the Natural Sciences and Engineering Research Council of Canada (Discovery grants to J.A.O. and R.P.P.), by the Alberta Agriculture Research Institute (grant to J.A.O. and D.M.R.), and by a Commonwealth Scholarship (to A.D.W.).</p></fn><fn id="afn1" fn-type="equal"><label>2</label><p>These authors contributed equally to the article.</p></fn><corresp id="cor1"><label>*</label>Address correspondence to <email>jocelyn.ozga@ualberta.ca</email>.</corresp><fn id="afn2"><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Dennis M. Reinecke (<email>dennis.reinecke@ualberta.ca</email>).</p></fn><fn id="fn2"><label>[W]</label><p>The online version of this article contains Web-only data.</p></fn><fn id="fn3"><label>[OPEN]</label><p>Articles can be viewed online without a subscription.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org/cgi/doi/10.1104/pp.113.225987">www.plantphysiol.org/cgi/doi/10.1104/pp.113.225987</ext-link></p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher
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<pub-date pub-type="ppub"><month>10</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>26</day><month>8</month><year>2013</year></pub-date><pub-date pub-type="pmc-release"><day>26</day><month>8</month><year>2013</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>163</volume><issue>2</issue><fpage>929</fpage><lpage>945</lpage><history><date date-type="received"><day>31</day><month>7</month><year>2013</year></date><date date-type="accepted"><day>21</day><month>8</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 American Society of Plant Biologists. All Rights Reserved.</copyright-statement><copyright-year>2013</copyright-year></permissions><self-uri xlink:role="icon" xlink:type="simple" xlink:href="PP_225987_ic1.gif"></self-uri><abstract abstract-type="precis"><p><italic>GA biosynthesis and catabolism regulation at the plant tissue and organ level is important for the timing/localization of gene expression for the production of bioactive GA, and thereby plant growth</italic>.</p></abstract><abstract><p>Gibberellins (GAs) are key modulators of plant growth and development. <italic>PsGA3ox1</italic> (<italic>LE</italic>) encodes a GA 3β-hydroxylase that catalyzes the conversion of GA<sub>20</sub> to biologically active GA<sub>1</sub>. To further clarify the role of <italic>GA3ox</italic> expression during pea (<italic>Pisum sativum</italic>) plant growth and development, we generated transgenic pea lines (in a <italic>lele</italic> background) with cauliflower mosaic virus-35S-driven expression of <italic>PsGA3ox1</italic> (<italic>LE</italic>). <italic>PsGA3ox1</italic> transgene expression led to higher GA<sub>1</sub> concentrations in a tissue-specific and development-specific manner, altering GA biosynthesis and catabolism gene expression and plant phenotype. <italic>PsGA3ox1</italic> transgenic plants had longer internodes, tendrils, and fruits, larger stipules, and displayed delayed flowering, increased apical meristem life, and altered vascular development relative to the null controls. Transgenic <italic>PsGA3ox1</italic> overexpression lines were then compared with lines where endogenous <italic>PsGA3ox1</italic> (<italic>LE</italic>) was introduced, by a series of backcrosses, into the same genetic background (BC <italic>LEle</italic>). Most notably, the BC <italic>LEle</italic> plants had substantially longer internodes containing much greater GA<sub>1</sub> levels than the transgenic <italic>PsGA3ox1</italic> plants. Induction of expression of the GA deactivation gene <italic>PsGA2ox1</italic> appears to make an important contribution to limiting the increase of internode GA<sub>1</sub> to modest levels for the transgenic lines. In contrast, <italic>PsGA3ox1</italic> (<italic>LE</italic>) expression driven by its endogenous promoter was coordinated within the internode tissue to avoid feed-forward regulation of <italic>PsGA2ox1</italic>, resulting in much greater GA<sub>1</sub> accumulation. These studies further our fundamental understanding of the regulation of GA biosynthesis and catabolism at the tissue and organ level and demonstrate that the timing/localization of <italic>GA3ox</italic> expression within an organ affects both GA homeostasis and GA<sub>1</sub> levels, and thereby growth.</p></abstract><counts><page-count count="17"></page-count></counts></article-meta><notes><glossary><title>Glossary</title><def-list><def-item><term id="term1">CaMV</term><def id="def1"><p>cauliflower mosaic virus</p></def></def-item><def-item><term id="term2">DAA</term><def id="def2"><p>days after anthesis</p></def></def-item><def-item><term id="term3">ABA</term><def id="def3"><p>abscisic acid</p></def></def-item><def-item><term id="term4">qRT</term><def id="def4"><p>quantitative reverse transcription</p></def></def-item><def-item><term id="term5">Ct</term><def id="def5"><p>cycle threshold</p></def></def-item></def-list></glossary></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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