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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Redox 2-Cys Mechanism Regulates the Catalytic Activity of Divergent Cyclophilins<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[W]</sup></xref></title><author><name sortKey="Campos, Bruna Medeia" sort="Campos, Bruna Medeia" uniqKey="Campos B" first="Bruna Medéia" last="Campos">Bruna Medéia Campos</name></author><author><name sortKey="Sforca, Mauricio Luis" sort="Sforca, Mauricio Luis" uniqKey="Sforca M" first="Mauricio Luis" last="Sforça">Mauricio Luis Sforça</name></author><author><name sortKey="Ambrosio, Andre Luis Berteli" sort="Ambrosio, Andre Luis Berteli" uniqKey="Ambrosio A" first="Andre Luis Berteli" last="Ambrosio">Andre Luis Berteli Ambrosio</name></author><author><name sortKey="Domingues, Mariane Noronha" sort="Domingues, Mariane Noronha" uniqKey="Domingues M" first="Mariane Noronha" last="Domingues">Mariane Noronha Domingues</name></author><author><name sortKey="Brasil De Souza, Tatiana De Arruda Campos" sort="Brasil De Souza, Tatiana De Arruda Campos" uniqKey="Brasil De Souza T" first="Tatiana De Arruda Campos" last="Brasil De Souza">Tatiana De Arruda Campos Brasil De Souza</name></author><author><name sortKey="Barbosa, Joao Alexandre Ribeiro Goncalvez" sort="Barbosa, Joao Alexandre Ribeiro Goncalvez" uniqKey="Barbosa J" first="João Alexandre Ribeiro Gonçalvez" last="Barbosa">João Alexandre Ribeiro Gonçalvez Barbosa</name></author><author><name sortKey="Leme, Adriana Franco Paes" sort="Leme, Adriana Franco Paes" uniqKey="Leme A" first="Adriana Franco Paes" last="Leme">Adriana Franco Paes Leme</name></author><author><name sortKey="Perez, Carlos Alberto" sort="Perez, Carlos Alberto" uniqKey="Perez C" first="Carlos Alberto" last="Perez">Carlos Alberto Perez</name></author><author><name sortKey="Whittaker, Sara Britt Marie" sort="Whittaker, Sara Britt Marie" uniqKey="Whittaker S" first="Sara Britt-Marie" last="Whittaker">Sara Britt-Marie Whittaker</name></author><author><name sortKey="Murakami, Mario Tyago" sort="Murakami, Mario Tyago" uniqKey="Murakami M" first="Mario Tyago" last="Murakami">Mario Tyago Murakami</name></author><author><name sortKey="Zeri, Ana Carolina De Matos" sort="Zeri, Ana Carolina De Matos" uniqKey="Zeri A" first="Ana Carolina De Matos" last="Zeri">Ana Carolina De Matos Zeri</name></author><author><name sortKey="Benedetti, Celso Eduardo" sort="Benedetti, Celso Eduardo" uniqKey="Benedetti C" first="Celso Eduardo" last="Benedetti">Celso Eduardo Benedetti</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23709667</idno><idno type="pmc">3707534</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707534</idno><idno type="RBID">PMC:3707534</idno><idno type="doi">10.1104/pp.113.218339</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000D08</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Redox 2-Cys Mechanism Regulates the Catalytic Activity of Divergent Cyclophilins<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[W]</sup></xref></title><author><name sortKey="Campos, Bruna Medeia" sort="Campos, Bruna Medeia" uniqKey="Campos B" first="Bruna Medéia" last="Campos">Bruna Medéia Campos</name></author><author><name sortKey="Sforca, Mauricio Luis" sort="Sforca, Mauricio Luis" uniqKey="Sforca M" first="Mauricio Luis" last="Sforça">Mauricio Luis Sforça</name></author><author><name sortKey="Ambrosio, Andre Luis Berteli" sort="Ambrosio, Andre Luis Berteli" uniqKey="Ambrosio A" first="Andre Luis Berteli" last="Ambrosio">Andre Luis Berteli Ambrosio</name></author><author><name sortKey="Domingues, Mariane Noronha" sort="Domingues, Mariane Noronha" uniqKey="Domingues M" first="Mariane Noronha" last="Domingues">Mariane Noronha Domingues</name></author><author><name sortKey="Brasil De Souza, Tatiana De Arruda Campos" sort="Brasil De Souza, Tatiana De Arruda Campos" uniqKey="Brasil De Souza T" first="Tatiana De Arruda Campos" last="Brasil De Souza">Tatiana De Arruda Campos Brasil De Souza</name></author><author><name sortKey="Barbosa, Joao Alexandre Ribeiro Goncalvez" sort="Barbosa, Joao Alexandre Ribeiro Goncalvez" uniqKey="Barbosa J" first="João Alexandre Ribeiro Gonçalvez" last="Barbosa">João Alexandre Ribeiro Gonçalvez Barbosa</name></author><author><name sortKey="Leme, Adriana Franco Paes" sort="Leme, Adriana Franco Paes" uniqKey="Leme A" first="Adriana Franco Paes" last="Leme">Adriana Franco Paes Leme</name></author><author><name sortKey="Perez, Carlos Alberto" sort="Perez, Carlos Alberto" uniqKey="Perez C" first="Carlos Alberto" last="Perez">Carlos Alberto Perez</name></author><author><name sortKey="Whittaker, Sara Britt Marie" sort="Whittaker, Sara Britt Marie" uniqKey="Whittaker S" first="Sara Britt-Marie" last="Whittaker">Sara Britt-Marie Whittaker</name></author><author><name sortKey="Murakami, Mario Tyago" sort="Murakami, Mario Tyago" uniqKey="Murakami M" first="Mario Tyago" last="Murakami">Mario Tyago Murakami</name></author><author><name sortKey="Zeri, Ana Carolina De Matos" sort="Zeri, Ana Carolina De Matos" uniqKey="Zeri A" first="Ana Carolina De Matos" last="Zeri">Ana Carolina De Matos Zeri</name></author><author><name sortKey="Benedetti, Celso Eduardo" sort="Benedetti, Celso Eduardo" uniqKey="Benedetti C" first="Celso Eduardo" last="Benedetti">Celso Eduardo Benedetti</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A novel type of allosteric regulation in divergent cyclophilins engages disulfide bond formation and a loop-displacement mechanism.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Plant Physiol</journal-id><journal-id journal-id-type="hwp">plantphysiol</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>Plant Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23709667</article-id><article-id pub-id-type="pmc">3707534</article-id><article-id pub-id-type="publisher-id">218339</article-id><article-id pub-id-type="doi">10.1104/pp.113.218339</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biochemistry and Metabolism</subject></subj-group></article-categories><title-group><article-title>A Redox 2-Cys Mechanism Regulates the Catalytic Activity of Divergent Cyclophilins<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[W]</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Campos</surname><given-names>Bruna Medéia</given-names></name></contrib><contrib contrib-type="author"><name><surname>Sforça</surname><given-names>Mauricio Luis</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ambrosio</surname><given-names>Andre Luis Berteli</given-names></name></contrib><contrib contrib-type="author"><name><surname>Domingues</surname><given-names>Mariane Noronha</given-names></name></contrib><contrib contrib-type="author"><name><surname>Brasil de Souza</surname><given-names>Tatiana de Arruda Campos</given-names></name><xref ref-type="author-notes" rid="afn1"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Barbosa</surname><given-names>João Alexandre Ribeiro Gonçalvez</given-names></name><xref ref-type="author-notes" rid="afn2"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Leme</surname><given-names>Adriana Franco Paes</given-names></name></contrib><contrib contrib-type="author"><name><surname>Perez</surname><given-names>Carlos Alberto</given-names></name></contrib><contrib contrib-type="author"><name><surname>Whittaker</surname><given-names>Sara Britt-Marie</given-names></name></contrib><contrib contrib-type="author"><name><surname>Murakami</surname><given-names>Mario Tyago</given-names></name></contrib><contrib contrib-type="author"><name><surname>Zeri</surname><given-names>Ana Carolina de Matos</given-names></name></contrib><contrib contrib-type="author"><name><surname>Benedetti</surname><given-names>Celso Eduardo</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff id="aff1">Laboratório Nacional de Biociências (B.M.C., M.L.S., A.L.B.A., M.N.D., T.d.A.C.B.d.S., J.A.R.G.B., A.F.P.L., M.T.M., A.C.d.M.Z., C.E.B.) and Laboratório Nacional de Luz Síncrotron (C.A.P.), Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP CP6192, Brazil; and</aff><aff id="aff2">School of Cancer Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom (S.B.-M.W.)</aff></contrib-group><author-notes><fn id="fn1" fn-type="supported-by"><label>1</label><p>This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (fellowship to B.M.C.) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (fellowship to C.E.B.).</p></fn><fn id="afn1" fn-type="present-address"><label>2</label><p>Present address: Instituto Carlos Chagas, FIOCRUZ-Paraná, Curitiba, PR, CEP 81350–010, Brazil.</p></fn><fn id="afn2" fn-type="present-address"><label>3</label><p>Present address: Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasilia, DF, CEP 70910–900, Brazil.</p></fn><corresp id="cor1"><label>*</label>Corresponding author; e-mail <email>celso.benedetti@lnbio.cnpem.br</email>.</corresp><fn id="afn3"><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Celso Eduardo Benedetti (<email>celso.benedetti@lnbio.cnpem.br</email>).</p></fn><fn id="fn2"><label>[W]</label><p>The online version of this article contains Web-only data.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org/cgi/doi/10.1104/pp.113.218339">www.plantphysiol.org/cgi/doi/10.1104/pp.113.218339</ext-link></p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher
pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>7</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>24</day><month>5</month><year>2013</year></pub-date><volume>162</volume><issue>3</issue><fpage>1311</fpage><lpage>1323</lpage><history><date date-type="received"><day>22</day><month>3</month><year>2013</year></date><date date-type="accepted"><day>23</day><month>5</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 American Society of Plant Biologists. All Rights Reserved.</copyright-statement><copyright-year>2013</copyright-year></permissions><self-uri xlink:role="icon" xlink:type="simple" xlink:href="PP_218339_ic1.gif"></self-uri><abstract abstract-type="precis"><p>A novel type of allosteric regulation in divergent cyclophilins engages disulfide bond formation and a loop-displacement mechanism.</p></abstract><abstract><p>The citrus (<italic>Citrus sinensis</italic>) cyclophilin CsCyp is a target of the <italic>Xanthomonas citri</italic> transcription activator-like effector PthA, required to elicit cankers on citrus. CsCyp binds the citrus thioredoxin CsTdx and the carboxyl-terminal domain of RNA polymerase II and is a divergent cyclophilin that carries the additional loop KSGKPLH, invariable cysteine (Cys) residues Cys-40 and Cys-168, and the conserved glutamate (Glu) Glu-83. Despite the suggested roles in ATP and metal binding, the functions of these unique structural elements remain unknown. Here, we show that the conserved Cys residues form a disulfide bond that inactivates the enzyme, whereas Glu-83, which belongs to the catalytic loop and is also critical for enzyme activity, is anchored to the divergent loop to maintain the active site open. In addition, we demonstrate that Cys-40 and Cys-168 are required for the interaction with CsTdx and that CsCyp binds the citrus carboxyl-terminal domain of RNA polymerase II YSPSAP repeat. Our data support a model where formation of the Cys-40-Cys-168 disulfide bond induces a conformational change that disrupts the interaction of the divergent and catalytic loops, via Glu-83, causing the active site to close. This suggests a new type of allosteric regulation in divergent cyclophilins, involving disulfide bond formation and a loop-displacement mechanism.</p></abstract><counts><page-count count="13"></page-count></counts></article-meta><notes><glossary><title>Glossary</title><def-list><def-item><term id="term1">Cyp</term><def id="def1"><p>cyclophilin</p></def></def-item><def-item><term id="term2">PPIase</term><def id="def2"><p>peptidyl prolyl cis-trans-isomerase</p></def></def-item><def-item><term id="term3">CsA</term><def id="def3"><p>cyclosporin A</p></def></def-item><def-item><term id="term4">CTD</term><def id="def4"><p>C-terminal domain of citrus RNA polymerase II</p></def></def-item><def-item><term id="term5">3D</term><def id="def5"><p>three-dimensional</p></def></def-item><def-item><term id="term6">GST</term><def id="def6"><p>glutathione <italic>S</italic>-transferase</p></def></def-item><def-item><term id="term7"><italic>K</italic><sub>d</sub></term><def id="def7"><p>dissociation constant</p></def></def-item><def-item><term id="term8">FF</term><def id="def8"><p>fully folded</p></def></def-item><def-item><term id="term9">LU</term><def id="def9"><p>locally unfolded</p></def></def-item><def-item><term id="term10">IPTG</term><def id="def10"><p>isopropyl-thio-β-<sc>d</sc>-galactopyranoside</p></def></def-item><def-item><term id="term11">PBS</term><def id="def11"><p>phosphate-buffered saline</p></def></def-item><def-item><term id="term12">DTT</term><def id="def12"><p>dithiothreitol</p></def></def-item></def-list></glossary></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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