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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Chromoplast-Specific Carotenoid-Associated Protein Appears to Be Important for Enhanced Accumulation of Carotenoids in <italic>hp1</italic> Tomato Fruits<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[C]</sup></xref><xref ref-type="author-notes" rid="fn3"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn4"><sup>[OA]</sup></xref></title><author><name sortKey="Kilambi, Himabindu Vasuki" sort="Kilambi, Himabindu Vasuki" uniqKey="Kilambi H" first="Himabindu Vasuki" last="Kilambi">Himabindu Vasuki Kilambi</name></author><author><name sortKey="Kumar, Rakesh" sort="Kumar, Rakesh" uniqKey="Kumar R" first="Rakesh" last="Kumar">Rakesh Kumar</name></author><author><name sortKey="Sharma, Rameshwar" sort="Sharma, Rameshwar" uniqKey="Sharma R" first="Rameshwar" last="Sharma">Rameshwar Sharma</name></author><author><name sortKey="Sreelakshmi, Yellamaraju" sort="Sreelakshmi, Yellamaraju" uniqKey="Sreelakshmi Y" first="Yellamaraju" last="Sreelakshmi">Yellamaraju Sreelakshmi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23400702</idno><idno type="pmc">3613478</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613478</idno><idno type="RBID">PMC:3613478</idno><idno type="doi">10.1104/pp.112.212191</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000D06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Chromoplast-Specific Carotenoid-Associated Protein Appears to Be Important for Enhanced Accumulation of Carotenoids in <italic>hp1</italic> Tomato Fruits<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[C]</sup></xref><xref ref-type="author-notes" rid="fn3"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn4"><sup>[OA]</sup></xref></title><author><name sortKey="Kilambi, Himabindu Vasuki" sort="Kilambi, Himabindu Vasuki" uniqKey="Kilambi H" first="Himabindu Vasuki" last="Kilambi">Himabindu Vasuki Kilambi</name></author><author><name sortKey="Kumar, Rakesh" sort="Kumar, Rakesh" uniqKey="Kumar R" first="Rakesh" last="Kumar">Rakesh Kumar</name></author><author><name sortKey="Sharma, Rameshwar" sort="Sharma, Rameshwar" uniqKey="Sharma R" first="Rameshwar" last="Sharma">Rameshwar Sharma</name></author><author><name sortKey="Sreelakshmi, Yellamaraju" sort="Sreelakshmi, Yellamaraju" uniqKey="Sreelakshmi Y" first="Yellamaraju" last="Sreelakshmi">Yellamaraju Sreelakshmi</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A chromoplast protein may assist in the sequestration and stabilization of carotenoids.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Plant Physiol</journal-id><journal-id journal-id-type="hwp">plantphysiol</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>Plant Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23400702</article-id><article-id pub-id-type="pmc">3613478</article-id><article-id pub-id-type="publisher-id">212191</article-id><article-id pub-id-type="doi">10.1104/pp.112.212191</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biochemistry and Metabolism</subject></subj-group></article-categories><title-group><article-title>Chromoplast-Specific Carotenoid-Associated Protein Appears to Be Important for Enhanced Accumulation of Carotenoids in <italic>hp1</italic> Tomato Fruits<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn2"><sup>[C]</sup></xref><xref ref-type="author-notes" rid="fn3"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn4"><sup>[OA]</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kilambi</surname><given-names>Himabindu Vasuki</given-names></name></contrib><contrib contrib-type="author"><name><surname>Kumar</surname><given-names>Rakesh</given-names></name></contrib><contrib contrib-type="author"><name><surname>Sharma</surname><given-names>Rameshwar</given-names></name></contrib><contrib contrib-type="author"><name><surname>Sreelakshmi</surname><given-names>Yellamaraju</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff id="aff1">Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India</aff></contrib-group><author-notes><corresp id="cor1"><label>*</label>Corresponding author; e-mail <email>syellamaraju@gmail.com</email>.</corresp><fn id="fn1" fn-type="supported-by"><label>1</label><p>This work was supported by the Department of Biotechnology, Government of India (grant no. BT/PR10903/GBD/27/123/2008 to Y.S.), the Department of Biotechnology, Tomato Metabolome Network, Government of India (grant no. BT/PR11671/PBD/16/828/2008 to Y.S. and R.S.), the University Grants Commission, New Delhi (junior research fellowship to H.V.K.), and the Council of Scientific and Industrial Research, New Delhi (senior research fellowship to R.K.).</p></fn><fn id="afn1"><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Yellamaraju Sreelakshmi (<email>syellamaraju@gmail.com</email>).</p></fn><fn id="fn2"><label>[C]</label><p>Some figures in this article are displayed in color online but in black and white in the print edition.</p></fn><fn id="fn3"><label>[W]</label><p>The online version of this article contains Web-only data.</p></fn><fn id="fn4"><label>[OA]</label><p>Open Access articles can be viewed online without a subscription.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org/cgi/doi/10.1104/pp.112.212191">www.plantphysiol.org/cgi/doi/10.1104/pp.112.212191</ext-link></p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>4</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>11</day><month>2</month><year>2013</year></pub-date><pub-date pub-type="pmc-release"><day>11</day><month>2</month><year>2013</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>161</volume><issue>4</issue><fpage>2085</fpage><lpage>2101</lpage><history><date date-type="received"><day>03</day><month>12</month><year>2012</year></date><date date-type="accepted"><day>09</day><month>2</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 American Society of Plant Biologists. All Rights Reserved.</copyright-statement><copyright-year>2013</copyright-year></permissions><self-uri xlink:role="icon" xlink:type="simple" xlink:href="PP_212191_ic1.gif"></self-uri><abstract abstract-type="precis"><p>A chromoplast protein may assist in the sequestration and stabilization of carotenoids.</p></abstract><abstract><p>Tomato (<italic>Solanum lycopersicum</italic>) high-pigment mutants with lesions in diverse loci such as DNA Damage-Binding Protein1 (<italic>high pigment1</italic> [<italic>hp1</italic>]), Deetiolated1 (<italic>hp2</italic>), Zeaxanthin Epoxidase (<italic>hp3</italic>), and Intense pigment (<italic>Ip</italic>; gene product unknown) exhibit increased accumulation of fruit carotenoids coupled with an increase in chloroplast number and size. However, little is known about the underlying mechanisms exaggerating the carotenoid accumulation and the chloroplast number in these mutants. A comparison of proteome profiles from the outer pericarp of <italic>hp1</italic> mutant and wild-type (cv Ailsa Craig) fruits at different developmental stages revealed at least 72 differentially expressed proteins during ripening. Hierarchical clustering grouped these proteins into three clusters. We found an increased abundance of chromoplast-specific carotenoid-associated protein (CHRC) in <italic>hp1</italic> fruits at red-ripe stage that is also reflected in its transcript level. Western blotting using CHRC polyclonal antibody from bell pepper (<italic>Capsicum annuum</italic>) revealed a 2-fold increase in the abundance of CHRC protein in the red-ripe stage of <italic>hp1</italic> fruits compared with the wild type. CHRC levels in <italic>hp2</italic> were found to be similar to that of <italic>hp1</italic>, whereas <italic>hp3</italic> and <italic>Ip</italic> showed intermediate levels to those in <italic>hp1</italic>, <italic>hp2</italic>, and wild-type fruits. Both CHRC and carotenoids were present in the isolated plastoglobules. Overall, our results suggest that loss of function of <italic>DDB1</italic>, <italic>DET1</italic>, <italic>Zeaxanthin Epoxidase</italic>, and <italic>Ip</italic> up-regulates CHRC levels. Increase in CHRC levels may contribute to the enhanced carotenoid content in these high-pigment fruits by assisting in the sequestration and stabilization of carotenoids.</p></abstract><counts><page-count count="17"></page-count></counts></article-meta><notes><glossary><title>Glossary</title><def-list><def-item><term id="term1">RNAi</term><def id="def1"><p>RNA interference</p></def></def-item><def-item><term id="term2">MG</term><def id="def2"><p>mature green</p></def></def-item><def-item><term id="term3">RR</term><def id="def3"><p>red-ripe</p></def></def-item><def-item><term id="term4">BR</term><def id="def4"><p>breaker</p></def></def-item><def-item><term id="term5">MALDI</term><def id="def5"><p>matrix-assisted laser-desorption ionization</p></def></def-item><def-item><term id="term6">TOF</term><def id="def6"><p>time of flight</p></def></def-item><def-item><term id="term7">ABA</term><def id="def7"><p>abscisic acid</p></def></def-item><def-item><term id="term8">SGN</term><def id="def8"><p>Solanaceae Genome Network</p></def></def-item><def-item><term id="term9">AC</term><def id="def9"><p>cv Ailsa Craig</p></def></def-item><def-item><term id="term10">DTT</term><def id="def10"><p>dithiothreitol</p></def></def-item><def-item><term id="term11">PMSF</term><def id="def11"><p>phenylmethylsulfonyl fluoride</p></def></def-item><def-item><term id="term12">MS/MS</term><def id="def12"><p>tandem mass spectrometry</p></def></def-item><def-item><term id="term13"><italic>m/z</italic></term><def id="def13"><p>mass-to-charge ratio</p></def></def-item></def-list></glossary></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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