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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning and Functional Characterization of a Gene for Capsanthin-Capsorubin Synthase from Tiger Lily (<italic>Lilium lancifolium</italic> Thunb. ‘Splendens’)</title><author><name sortKey="Jekni, Zoran" sort="Jekni, Zoran" uniqKey="Jekni Z" first="Zoran" last="Jekni">Zoran Jekni</name><affiliation><nlm:aff id="pcs128-AFF1">Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA</nlm:aff></affiliation></author><author><name sortKey="Morre, Jeffrey T" sort="Morre, Jeffrey T" uniqKey="Morre J" first="Jeffrey T." last="Morré">Jeffrey T. Morré</name><affiliation><nlm:aff id="pcs128-AFF2">Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA</nlm:aff></affiliation></author><author><name sortKey="Jekni, Stevan" sort="Jekni, Stevan" uniqKey="Jekni S" first="Stevan" last="Jekni">Stevan Jekni</name><affiliation><nlm:aff id="pcs128-AFF1">Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA</nlm:aff></affiliation></author><author><name sortKey="Jevremovi, Sla Ana" sort="Jevremovi, Sla Ana" uniqKey="Jevremovi S" first="Sla Ana" last="Jevremovi">Sla Ana Jevremovi</name><affiliation><nlm:aff id="pcs128-AFF3">Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia</nlm:aff></affiliation></author><author><name sortKey="Suboti, Angelina" sort="Suboti, Angelina" uniqKey="Suboti A" first="Angelina" last="Suboti">Angelina Suboti</name><affiliation><nlm:aff id="pcs128-AFF3">Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia</nlm:aff></affiliation></author><author><name sortKey="Chen, Tony H H" sort="Chen, Tony H H" uniqKey="Chen T" first="Tony H. H." last="Chen">Tony H. H. Chen</name><affiliation><nlm:aff id="pcs128-AFF1">Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23008421</idno><idno type="pmc">3494009</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3494009</idno><idno type="RBID">PMC:3494009</idno><idno type="doi">10.1093/pcp/pcs128</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000C81</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cloning and Functional Characterization of a Gene for Capsanthin-Capsorubin Synthase from Tiger Lily (<italic>Lilium lancifolium</italic> Thunb. ‘Splendens’)</title><author><name sortKey="Jekni, Zoran" sort="Jekni, Zoran" uniqKey="Jekni Z" first="Zoran" last="Jekni">Zoran Jekni</name><affiliation><nlm:aff id="pcs128-AFF1">Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA</nlm:aff></affiliation></author><author><name sortKey="Morre, Jeffrey T" sort="Morre, Jeffrey T" uniqKey="Morre J" first="Jeffrey T." last="Morré">Jeffrey T. Morré</name><affiliation><nlm:aff id="pcs128-AFF2">Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA</nlm:aff></affiliation></author><author><name sortKey="Jekni, Stevan" sort="Jekni, Stevan" uniqKey="Jekni S" first="Stevan" last="Jekni">Stevan Jekni</name><affiliation><nlm:aff id="pcs128-AFF1">Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA</nlm:aff></affiliation></author><author><name sortKey="Jevremovi, Sla Ana" sort="Jevremovi, Sla Ana" uniqKey="Jevremovi S" first="Sla Ana" last="Jevremovi">Sla Ana Jevremovi</name><affiliation><nlm:aff id="pcs128-AFF3">Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia</nlm:aff></affiliation></author><author><name sortKey="Suboti, Angelina" sort="Suboti, Angelina" uniqKey="Suboti A" first="Angelina" last="Suboti">Angelina Suboti</name><affiliation><nlm:aff id="pcs128-AFF3">Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia</nlm:aff></affiliation></author><author><name sortKey="Chen, Tony H H" sort="Chen, Tony H H" uniqKey="Chen T" first="Tony H. H." last="Chen">Tony H. H. Chen</name><affiliation><nlm:aff id="pcs128-AFF1">Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA</nlm:aff></affiliation></author></analytic><series><title level="j">Plant and Cell Physiology</title><idno type="ISSN">0032-0781</idno><idno type="eISSN">1471-9053</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The orange color of tiger lily (<italic>Lolium lancifolium</italic> ‘Splendens’) flowers is due, primarily, to the accumulation of two κ-xanthophylls, capsanthin and capsorubin. An enzyme, known as capsanthin-capsorubin synthase (CCS), catalyzes the conversion of antheraxanthin and violaxanthin into capsanthin and capsorubin, respectively. We cloned the gene for capsanthin-capsorubin synthase (<italic>Llccs</italic>) from flower tepals of <italic>L</italic>. <italic>lancifolium</italic> by the rapid amplification of cDNA ends (RACE) with a heterologous non-degenerate primer that was based on the sequence of a gene for lycopene β-cyclase (<italic>lcyB</italic>). The full-length cDNA of <italic>Llccs</italic> was 1,785 bp long and contained an open reading frame of 1,425 bp that encoded a polypeptide of 474 amino acids with a predicted N-terminal plastid-targeting sequence. Analysis by reverse transcription–PCR (RT–PCR) revealed that expression of <italic>Llccs</italic> was spatially and temporally regulated, with expression in flower buds and flowers of <italic>L</italic>. <italic>lancifolium</italic> but not in vegetative tissues. Stable overexpression of the <italic>Llccs</italic> gene in callus tissue of <italic>Iris germanica</italic>, which accumulates several xanthophylls including violaxanthin, the precursor of capsorubin, resulted in transgenic callus whose color had changed from its normal yellow to red-orange. This novel red-orange coloration was due to the accumulation of two non-native κ-xanthophylls, capsanthin and capsorubin, as confirmed by HPLC and ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis with authentic standards. Cloning of the <italic>Llccs</italic> gene should advance our understanding of the molecular and genetic mechanisms of the biosynthesis of κ-carotenoids in general and in the genus <italic>Lilium</italic> in particular, and will facilitate transgenic alterations of the colors of flowers and fruits of many plant species.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Plant Cell Physiol</journal-id><journal-id journal-id-type="publisher-id">pcp</journal-id><journal-id journal-id-type="hwp">pcellphys</journal-id><journal-title-group><journal-title>Plant and Cell Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0781</issn><issn pub-type="epub">1471-9053</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23008421</article-id><article-id pub-id-type="pmc">3494009</article-id><article-id pub-id-type="doi">10.1093/pcp/pcs128</article-id><article-id pub-id-type="publisher-id">pcs128</article-id><article-categories><subj-group subj-group-type="heading"><subject>Regular Papers</subject></subj-group></article-categories><title-group><article-title>Cloning and Functional Characterization of a Gene for Capsanthin-Capsorubin Synthase from Tiger Lily (<italic>Lilium lancifolium</italic> Thunb. ‘Splendens’)</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Jeknić</surname><given-names>Zoran</given-names></name><xref ref-type="aff" rid="pcs128-AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Morré</surname><given-names>Jeffrey T.</given-names></name><xref ref-type="aff" rid="pcs128-AFF2"><sup>2</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Jeknić</surname><given-names>Stevan</given-names></name><xref ref-type="aff" rid="pcs128-AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Jevremović</surname><given-names>Slađana</given-names></name><xref ref-type="aff" rid="pcs128-AFF3"><sup>3</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Subotić</surname><given-names>Angelina</given-names></name><xref ref-type="aff" rid="pcs128-AFF3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Tony H.H.</given-names></name><xref ref-type="aff" rid="pcs128-AFF1"><sup>1</sup></xref><xref ref-type="corresp" rid="pcs128-COR1">*</xref></contrib></contrib-group><aff id="pcs128-AFF1"><sup>1</sup>Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA</aff><aff id="pcs128-AFF2"><sup>2</sup>Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA</aff><aff id="pcs128-AFF3"><sup>3</sup>Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia</aff><author-notes><corresp id="pcs128-COR1">*Corresponding author: E-mail, <email>chent@hort.oregonstate.edu</email>; Fax, <fax>+1-541-737-3479.</fax></corresp></author-notes><pub-date pub-type="ppub"><month>11</month><year>2012</year></pub-date><pub-date pub-type="epub"><day>24</day><month>9</month><year>2012</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>11</month><year>2013</year></pub-date><pmc-comment> PMC Release delay is 12 months and 0 days and was based on the
<volume>53</volume><issue>11</issue><fpage>1899</fpage><lpage>1912</lpage><history><date date-type="received"><day>1</day><month>5</month><year>2012</year></date><date date-type="accepted"><day>17</day><month>9</month><year>2012</year></date></history><permissions><copyright-statement>© The Author 2012. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com</copyright-statement><copyright-year>2012</copyright-year></permissions><abstract><p>The orange color of tiger lily (<italic>Lolium lancifolium</italic> ‘Splendens’) flowers is due, primarily, to the accumulation of two κ-xanthophylls, capsanthin and capsorubin. An enzyme, known as capsanthin-capsorubin synthase (CCS), catalyzes the conversion of antheraxanthin and violaxanthin into capsanthin and capsorubin, respectively. We cloned the gene for capsanthin-capsorubin synthase (<italic>Llccs</italic>) from flower tepals of <italic>L</italic>. <italic>lancifolium</italic> by the rapid amplification of cDNA ends (RACE) with a heterologous non-degenerate primer that was based on the sequence of a gene for lycopene β-cyclase (<italic>lcyB</italic>). The full-length cDNA of <italic>Llccs</italic> was 1,785 bp long and contained an open reading frame of 1,425 bp that encoded a polypeptide of 474 amino acids with a predicted N-terminal plastid-targeting sequence. Analysis by reverse transcription–PCR (RT–PCR) revealed that expression of <italic>Llccs</italic> was spatially and temporally regulated, with expression in flower buds and flowers of <italic>L</italic>. <italic>lancifolium</italic> but not in vegetative tissues. Stable overexpression of the <italic>Llccs</italic> gene in callus tissue of <italic>Iris germanica</italic>, which accumulates several xanthophylls including violaxanthin, the precursor of capsorubin, resulted in transgenic callus whose color had changed from its normal yellow to red-orange. This novel red-orange coloration was due to the accumulation of two non-native κ-xanthophylls, capsanthin and capsorubin, as confirmed by HPLC and ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis with authentic standards. Cloning of the <italic>Llccs</italic> gene should advance our understanding of the molecular and genetic mechanisms of the biosynthesis of κ-carotenoids in general and in the genus <italic>Lilium</italic> in particular, and will facilitate transgenic alterations of the colors of flowers and fruits of many plant species.</p></abstract><kwd-group><kwd>Antheraxanthin</kwd><kwd>Capsanthin</kwd><kwd>Capsorubin</kwd><kwd>Carotenoids</kwd><kwd>Lycopene β-cyclase</kwd><kwd>Violaxanthin</kwd></kwd-group><counts><page-count count="14"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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