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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Peptide Aptamers That Bind to Geminivirus Replication Proteins Confer a Resistance Phenotype to <italic>Tomato Yellow Leaf Curl Virus</italic> and <italic>Tomato Mottle Virus</italic> Infection in Tomato</title><author><name sortKey="Reyes, Maria Ines" sort="Reyes, Maria Ines" uniqKey="Reyes M" first="Maria Ines" last="Reyes">Maria Ines Reyes</name></author><author><name sortKey="Nash, Tara E" sort="Nash, Tara E" uniqKey="Nash T" first="Tara E." last="Nash">Tara E. Nash</name></author><author><name sortKey="Dallas, Mary M" sort="Dallas, Mary M" uniqKey="Dallas M" first="Mary M." last="Dallas">Mary M. Dallas</name></author><author><name sortKey="Ascencio Iba Ez, J Trinidad" sort="Ascencio Iba Ez, J Trinidad" uniqKey="Ascencio Iba Ez J" first="J. Trinidad" last="Ascencio-Ibá Ez">J. Trinidad Ascencio-Ibá Ez</name></author><author><name sortKey="Hanley Bowdoin, Linda" sort="Hanley Bowdoin, Linda" uniqKey="Hanley Bowdoin L" first="Linda" last="Hanley-Bowdoin">Linda Hanley-Bowdoin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23824791</idno><idno type="pmc">3754110</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754110</idno><idno type="RBID">PMC:3754110</idno><idno type="doi">10.1128/JVI.01095-13</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000C70</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Peptide Aptamers That Bind to Geminivirus Replication Proteins Confer a Resistance Phenotype to <italic>Tomato Yellow Leaf Curl Virus</italic> and <italic>Tomato Mottle Virus</italic> Infection in Tomato</title><author><name sortKey="Reyes, Maria Ines" sort="Reyes, Maria Ines" uniqKey="Reyes M" first="Maria Ines" last="Reyes">Maria Ines Reyes</name></author><author><name sortKey="Nash, Tara E" sort="Nash, Tara E" uniqKey="Nash T" first="Tara E." last="Nash">Tara E. Nash</name></author><author><name sortKey="Dallas, Mary M" sort="Dallas, Mary M" uniqKey="Dallas M" first="Mary M." last="Dallas">Mary M. Dallas</name></author><author><name sortKey="Ascencio Iba Ez, J Trinidad" sort="Ascencio Iba Ez, J Trinidad" uniqKey="Ascencio Iba Ez J" first="J. Trinidad" last="Ascencio-Ibá Ez">J. Trinidad Ascencio-Ibá Ez</name></author><author><name sortKey="Hanley Bowdoin, Linda" sort="Hanley Bowdoin, Linda" uniqKey="Hanley Bowdoin L" first="Linda" last="Hanley-Bowdoin">Linda Hanley-Bowdoin</name></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Geminiviruses constitute a large family of single-stranded DNA viruses that cause serious losses in important crops worldwide. They often exist in disease complexes and have high recombination and mutation rates, allowing them to adapt rapidly to new hosts and environments. Thus, an effective resistance strategy must be general in character and able to target multiple viruses. The geminivirus replication protein (Rep) is a good target for broad-based disease control because it is highly conserved and required for viral replication. In an earlier study, we identified a set of peptide aptamers that bind to Rep and reduce viral replication in cultured plant cells. In this study, we selected 16 of the peptide aptamers for further analysis in yeast two-hybrid assays. The results of these experiments showed that all 16 peptide aptamers interact with all or most of the Rep proteins from nine viruses representing the three major <italic>Geminiviridae</italic> genera and identified two peptide aptamers (A22 and A64) that interact strongly with different regions in the Rep N terminus. Transgenic tomato lines expressing A22 or A64 and inoculated with <italic>Tomato yellow leaf curl virus</italic> or <italic>Tomato mottle virus</italic> exhibited delayed viral DNA accumulation and often contained lower levels of viral DNA. Strikingly, the effect on symptoms was stronger, with many of the plants showing no symptoms or strongly attenuated symptoms. Together, these results established the efficacy of using Rep-binding peptide aptamers to develop crops that are resistant to diverse geminiviruses.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id><journal-id journal-id-type="iso-abbrev">J. Virol</journal-id><journal-id journal-id-type="hwp">jvi</journal-id><journal-id journal-id-type="pmc">jvi</journal-id><journal-id journal-id-type="publisher-id">JVI</journal-id><journal-title-group><journal-title>Journal of Virology</journal-title></journal-title-group><issn pub-type="ppub">0022-538X</issn><issn pub-type="epub">1098-5514</issn><publisher><publisher-name>American Society for Microbiology</publisher-name><publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23824791</article-id><article-id pub-id-type="pmc">3754110</article-id><article-id pub-id-type="publisher-id">01095-13</article-id><article-id pub-id-type="doi">10.1128/JVI.01095-13</article-id><article-categories><subj-group subj-group-type="heading"><subject>Vaccines and Antiviral Agents</subject></subj-group></article-categories><title-group><article-title>Peptide Aptamers That Bind to Geminivirus Replication Proteins Confer a Resistance Phenotype to <italic>Tomato Yellow Leaf Curl Virus</italic> and <italic>Tomato Mottle Virus</italic> Infection in Tomato</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Reyes</surname><given-names>Maria Ines</given-names></name></contrib><contrib contrib-type="author"><name><surname>Nash</surname><given-names>Tara E.</given-names></name><xref ref-type="author-notes" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Dallas</surname><given-names>Mary M.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ascencio-Ibáñez</surname><given-names>J. Trinidad</given-names></name></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Hanley-Bowdoin</surname><given-names>Linda</given-names></name></contrib><aff>Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina, USA</aff></contrib-group><author-notes><corresp id="cor1">Address correspondence to Linda Hanley-Bowdoin, <email>linda_hanley-bowdoin@ncsu.edu</email>.</corresp><fn id="fn1" fn-type="present-address"><label>*</label><p>Present address: Tara E. Nash, Department of Horticultural Science, North Carolina State University, Raleigh, North Carolina, USA.</p></fn></author-notes><pub-date pub-type="ppub"><month>9</month><year>2013</year></pub-date><volume>87</volume><issue>17</issue><fpage>9691</fpage><lpage>9706</lpage><history><date date-type="received"><day>22</day><month>4</month><year>2013</year></date><date date-type="accepted"><day>21</day><month>6</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013, American Society for Microbiology. All Rights Reserved.</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>American Society for Microbiology</copyright-holder></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zjv01713009691.pdf"></self-uri><abstract><p>Geminiviruses constitute a large family of single-stranded DNA viruses that cause serious losses in important crops worldwide. They often exist in disease complexes and have high recombination and mutation rates, allowing them to adapt rapidly to new hosts and environments. Thus, an effective resistance strategy must be general in character and able to target multiple viruses. The geminivirus replication protein (Rep) is a good target for broad-based disease control because it is highly conserved and required for viral replication. In an earlier study, we identified a set of peptide aptamers that bind to Rep and reduce viral replication in cultured plant cells. In this study, we selected 16 of the peptide aptamers for further analysis in yeast two-hybrid assays. The results of these experiments showed that all 16 peptide aptamers interact with all or most of the Rep proteins from nine viruses representing the three major <italic>Geminiviridae</italic> genera and identified two peptide aptamers (A22 and A64) that interact strongly with different regions in the Rep N terminus. Transgenic tomato lines expressing A22 or A64 and inoculated with <italic>Tomato yellow leaf curl virus</italic> or <italic>Tomato mottle virus</italic> exhibited delayed viral DNA accumulation and often contained lower levels of viral DNA. Strikingly, the effect on symptoms was stronger, with many of the plants showing no symptoms or strongly attenuated symptoms. Together, these results established the efficacy of using Rep-binding peptide aptamers to develop crops that are resistant to diverse geminiviruses.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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