Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains*
Identifieur interne : 000C64 ( Pmc/Corpus ); précédent : 000C63; suivant : 000C65Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains*
Auteurs : Michael L. Hayes ; Karolyn Giang ; Beniam Berhane ; R. Michael MulliganSource :
- The Journal of Biological Chemistry [ 0021-9258 ] ; 2013.
Abstract
Url:
DOI: 10.1074/jbc.M113.485755
PubMed: 24194514
PubMed Central: 3868764
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains<xref ref-type="fn" rid="FN1">*</xref></title><author><name sortKey="Hayes, Michael L" sort="Hayes, Michael L" uniqKey="Hayes M" first="Michael L." last="Hayes">Michael L. Hayes</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Giang, Karolyn" sort="Giang, Karolyn" uniqKey="Giang K" first="Karolyn" last="Giang">Karolyn Giang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Berhane, Beniam" sort="Berhane, Beniam" uniqKey="Berhane B" first="Beniam" last="Berhane">Beniam Berhane</name><affiliation><nlm:aff id="aff2">Chemistry, University of California, Irvine, California 92697</nlm:aff></affiliation></author><author><name sortKey="Mulligan, R Michael" sort="Mulligan, R Michael" uniqKey="Mulligan R" first="R. Michael" last="Mulligan">R. Michael Mulligan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24194514</idno><idno type="pmc">3868764</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868764</idno><idno type="RBID">PMC:3868764</idno><idno type="doi">10.1074/jbc.M113.485755</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000C64</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains<xref ref-type="fn" rid="FN1">*</xref></title><author><name sortKey="Hayes, Michael L" sort="Hayes, Michael L" uniqKey="Hayes M" first="Michael L." last="Hayes">Michael L. Hayes</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Giang, Karolyn" sort="Giang, Karolyn" uniqKey="Giang K" first="Karolyn" last="Giang">Karolyn Giang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Berhane, Beniam" sort="Berhane, Beniam" uniqKey="Berhane B" first="Beniam" last="Berhane">Beniam Berhane</name><affiliation><nlm:aff id="aff2">Chemistry, University of California, Irvine, California 92697</nlm:aff></affiliation></author><author><name sortKey="Mulligan, R Michael" sort="Mulligan, R Michael" uniqKey="Mulligan R" first="R. Michael" last="Mulligan">R. Michael Mulligan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">The Journal of Biological Chemistry</title><idno type="ISSN">0021-9258</idno><idno type="eISSN">1083-351X</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><bold>Background:</bold> Many pentatricopeptide repeat (PPR) proteins are RNA site specificity factors and include C-terminal DYW deaminase domains.</p><p><bold>Results:</bold> ELI1 and DOT4 are required for editing single sites. The DYW deaminase domain binds two zinc atoms.</p><p><bold>Conclusion:</bold> The C terminus of PLS-type PPR proteins shares molecular characteristics with cytidine deaminase.</p><p><bold>Significance:</bold> This study provides the first evidence that DYW deaminase domains bind zinc.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Biol Chem</journal-id><journal-id journal-id-type="iso-abbrev">J. Biol. Chem</journal-id><journal-id journal-id-type="hwp">jbc</journal-id><journal-id journal-id-type="pmc">jbc</journal-id><journal-id journal-id-type="publisher-id">JBC</journal-id><journal-title-group><journal-title>The Journal of Biological Chemistry</journal-title></journal-title-group><issn pub-type="ppub">0021-9258</issn><issn pub-type="epub">1083-351X</issn><publisher><publisher-name>American Society for Biochemistry and Molecular Biology</publisher-name><publisher-loc>9650 Rockville Pike, Bethesda, MD 20814, U.S.A.</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24194514</article-id><article-id pub-id-type="pmc">3868764</article-id><article-id pub-id-type="publisher-id">M113.485755</article-id><article-id pub-id-type="doi">10.1074/jbc.M113.485755</article-id><article-categories><subj-group subj-group-type="heading"><subject>Plant Biology</subject></subj-group></article-categories><title-group><article-title>Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains<xref ref-type="fn" rid="FN1">*</xref></article-title><alt-title alt-title-type="short">The DYW Domain Binds Two Zinc Atoms</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Hayes</surname><given-names>Michael L.</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Giang</surname><given-names>Karolyn</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Berhane</surname><given-names>Beniam</given-names></name><xref ref-type="aff" rid="aff2"><sup>§</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mulligan</surname><given-names>R. Michael</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><aff id="aff1">From the Departments of<label>‡</label>Developmental and Cell Biology and</aff><aff id="aff2"><label>§</label>Chemistry, University of California, Irvine, California 92697</aff></contrib-group><author-notes><corresp id="cor1"><label>1</label> To whom correspondence should be addressed: <addr-line>5219 McGaugh Hall, University of California, Irvine, CA 92697.</addr-line> Tel.: <phone>949-824-8433</phone>; Fax: <fax>949-824-4709</fax>; E-mail: <email>rmmullig@uci.edu</email>.</corresp></author-notes><pub-date pub-type="ppub"><day>20</day><month>12</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>5</day><month>11</month><year>2013</year></pub-date><volume>288</volume><issue>51</issue><fpage>36519</fpage><lpage>36529</lpage><history><date date-type="received"><day>15</day><month>5</month><year>2013</year></date><date date-type="rev-recd"><day>4</day><month>11</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.</copyright-statement><copyright-year>2013</copyright-year></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zbc05113036519.pdf"></self-uri><abstract abstract-type="teaser"><p><bold>Background:</bold> Many pentatricopeptide repeat (PPR) proteins are RNA site specificity factors and include C-terminal DYW deaminase domains.</p><p><bold>Results:</bold> ELI1 and DOT4 are required for editing single sites. The DYW deaminase domain binds two zinc atoms.</p><p><bold>Conclusion:</bold> The C terminus of PLS-type PPR proteins shares molecular characteristics with cytidine deaminase.</p><p><bold>Significance:</bold> This study provides the first evidence that DYW deaminase domains bind zinc.</p></abstract><abstract><p>Many transcripts expressed from plant organelle genomes are modified by C-to-U RNA editing. Nuclear encoded pentatricopeptide repeat (PPR) proteins are required as RNA binding specificity determinants in the RNA editing mechanism. Bioinformatic analysis has shown that most of the <italic>Arabidopsis</italic> PPR proteins necessary for RNA editing events include a C-terminal portion that shares structural characteristics with a superfamily of deaminases. The DYW deaminase domain includes a highly conserved zinc binding motif that shares characteristics with cytidine deaminases. The <italic>Arabidopsis</italic> PPR genes, <italic>ELI1</italic> and <italic>DOT4</italic>, both have DYW deaminase domains and are required for single RNA editing events in chloroplasts. The ELI1 DYW deaminase domain was expressed as a recombinant protein in <italic>Escherichia coli</italic> and was shown to bind two zinc atoms per polypeptide. Thus, the DYW deaminase domain binds a zinc metal ion, as expected for a cytidine deaminase, and is potentially the catalytic component of an editing complex. Genetic complementation experiments demonstrate that large portions of the DYW deaminase domain of <italic>ELI1</italic> may be eliminated, but the truncated genes retain the ability to restore editing site conversion in a mutant plant. These results suggest that the catalytic activity can be supplied in <italic>trans</italic> by uncharacterized protein(s) of the editosome.</p></abstract><kwd-group><kwd>Chloroplast</kwd><kwd>Gene Expression</kwd><kwd>Mass Spectrometry (MS)</kwd><kwd>Metalloenzymes</kwd><kwd>RNA Modification</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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