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In Vitro Photodynamic Inactivation of Plant-Pathogenic Fungi Colletotrichum acutatum and Colletotrichum gloeosporioides with Novel Phenothiazinium Photosensitizers

Identifieur interne : 000C34 ( Pmc/Corpus ); précédent : 000C33; suivant : 000C35

In Vitro Photodynamic Inactivation of Plant-Pathogenic Fungi Colletotrichum acutatum and Colletotrichum gloeosporioides with Novel Phenothiazinium Photosensitizers

Auteurs : Henrique D. De Menezes ; Gabriela B. Rodrigues ; Simone De Pádua Teixeira ; Nelson S. Massola ; Luciano Bachmann ; Mark Wainwright ; Gilberto U. L. Braga

Source :

Applied and Environmental Microbiology [ 0099-2240 ] ; 2014.

RBID : PMC:3957600

Abstract

The increasing tolerance to currently used fungicides in both clinical and agricultural areas is of great concern. The nonconventional light-based approach of antimicrobial photodynamic treatment (APDT) is a promising alternative to conventional fungicides. We evaluated the effects of APDT with four phenothiazinium derivatives (methylene blue [MB], new methylene blue N [NMBN], toluidine blue O [TBO], and the novel pentacyclic phenothiazinium photosensitizer [PS] S137) on conidia of three fungal species (Colletotrichum acutatum, Colletotrichum gloeosporioides, and Aspergillus nidulans). The efficacy of APDT with each PS was determined, initially, based on photosensitizer MICs. Additionally, the effects of APDT with two selected PSs (NMBN and S137) on survival of conidia were evaluated. The subcellular localization of the PS in C. acutatum conidia was determined. The effects of photodynamic treatments on leaves of the plant host Citrus sinensis were also investigated. APDT with S137 showed the lowest MIC. MICs for S137 were 5 μM for the three fungal species when a fluence of 25 J cm⁻² was used. APDT with NMBN (50 μM) and S137 (10 μM) resulted in a reduction in the survival of the conidia of all species of approximately 5 logs with fluences of ≥15 J cm⁻². Washing of the conidia before light exposure did not prevent photodynamic inactivation. Both NMBN and S137 accumulated in cytoplasmic structures, such as lipid bodies, of C. acutatum conidia. No damage to orange tree leaves was observed after APDT.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957600

DOI: 10.1128/AEM.02788-13
PubMed: 24362436
PubMed Central: 3957600

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PMC:3957600

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>In Vitro</italic>
 Photodynamic Inactivation of Plant-Pathogenic Fungi <named-content content-type="genus-species">Colletotrichum acutatum</named-content>
 and <named-content content-type="genus-species">Colletotrichum gloeosporioides</named-content>
 with Novel Phenothiazinium Photosensitizers</title>
<author><name sortKey="De Menezes, Henrique D" sort="De Menezes, Henrique D" uniqKey="De Menezes H" first="Henrique D." last="De Menezes">Henrique D. De Menezes</name>
<affiliation><nlm:aff id="aff1">Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Rodrigues, Gabriela B" sort="Rodrigues, Gabriela B" uniqKey="Rodrigues G" first="Gabriela B." last="Rodrigues">Gabriela B. Rodrigues</name>
<affiliation><nlm:aff id="aff1">Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Teixeira, Simone De Padua" sort="Teixeira, Simone De Padua" uniqKey="Teixeira S" first="Simone De Pádua" last="Teixeira">Simone De Pádua Teixeira</name>
<affiliation><nlm:aff id="aff2">Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Massola, Nelson S" sort="Massola, Nelson S" uniqKey="Massola N" first="Nelson S." last="Massola">Nelson S. Massola</name>
<affiliation><nlm:aff id="aff3">Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bachmann, Luciano" sort="Bachmann, Luciano" uniqKey="Bachmann L" first="Luciano" last="Bachmann">Luciano Bachmann</name>
<affiliation><nlm:aff id="aff4">Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wainwright, Mark" sort="Wainwright, Mark" uniqKey="Wainwright M" first="Mark" last="Wainwright">Mark Wainwright</name>
<affiliation><nlm:aff id="aff5">School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Braga, Gilberto U L" sort="Braga, Gilberto U L" uniqKey="Braga G" first="Gilberto U. L." last="Braga">Gilberto U. L. Braga</name>
<affiliation><nlm:aff id="aff1">Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff6">Research Support Center in Natural and Synthetic Products, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
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<idno type="doi">10.1128/AEM.02788-13</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000C34</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In Vitro</italic>
 Photodynamic Inactivation of Plant-Pathogenic Fungi <named-content content-type="genus-species">Colletotrichum acutatum</named-content>
 and <named-content content-type="genus-species">Colletotrichum gloeosporioides</named-content>
 with Novel Phenothiazinium Photosensitizers</title>
<author><name sortKey="De Menezes, Henrique D" sort="De Menezes, Henrique D" uniqKey="De Menezes H" first="Henrique D." last="De Menezes">Henrique D. De Menezes</name>
<affiliation><nlm:aff id="aff1">Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Rodrigues, Gabriela B" sort="Rodrigues, Gabriela B" uniqKey="Rodrigues G" first="Gabriela B." last="Rodrigues">Gabriela B. Rodrigues</name>
<affiliation><nlm:aff id="aff1">Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Teixeira, Simone De Padua" sort="Teixeira, Simone De Padua" uniqKey="Teixeira S" first="Simone De Pádua" last="Teixeira">Simone De Pádua Teixeira</name>
<affiliation><nlm:aff id="aff2">Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Massola, Nelson S" sort="Massola, Nelson S" uniqKey="Massola N" first="Nelson S." last="Massola">Nelson S. Massola</name>
<affiliation><nlm:aff id="aff3">Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bachmann, Luciano" sort="Bachmann, Luciano" uniqKey="Bachmann L" first="Luciano" last="Bachmann">Luciano Bachmann</name>
<affiliation><nlm:aff id="aff4">Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wainwright, Mark" sort="Wainwright, Mark" uniqKey="Wainwright M" first="Mark" last="Wainwright">Mark Wainwright</name>
<affiliation><nlm:aff id="aff5">School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Braga, Gilberto U L" sort="Braga, Gilberto U L" uniqKey="Braga G" first="Gilberto U. L." last="Braga">Gilberto U. L. Braga</name>
<affiliation><nlm:aff id="aff1">Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff6">Research Support Center in Natural and Synthetic Products, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><p>The increasing tolerance to currently used fungicides in both clinical and agricultural areas is of great concern. The nonconventional light-based approach of antimicrobial photodynamic treatment (APDT) is a promising alternative to conventional fungicides. We evaluated the effects of APDT with four phenothiazinium derivatives (methylene blue [MB], new methylene blue N [NMBN], toluidine blue O [TBO], and the novel pentacyclic phenothiazinium photosensitizer [PS] S137) on conidia of three fungal species (<named-content content-type="genus-species">Colletotrichum acutatum</named-content>
, <named-content content-type="genus-species">Colletotrichum gloeosporioides</named-content>
, and <named-content content-type="genus-species">Aspergillus nidulans</named-content>
). The efficacy of APDT with each PS was determined, initially, based on photosensitizer MICs. Additionally, the effects of APDT with two selected PSs (NMBN and S137) on survival of conidia were evaluated. The subcellular localization of the PS in <named-content content-type="genus-species">C. acutatum</named-content>
 conidia was determined. The effects of photodynamic treatments on leaves of the plant host <named-content content-type="genus-species">Citrus sinensis</named-content>
 were also investigated. APDT with S137 showed the lowest MIC. MICs for S137 were 5 μM for the three fungal species when a fluence of 25 J cm<sup>−2</sup>
 was used. APDT with NMBN (50 μM) and S137 (10 μM) resulted in a reduction in the survival of the conidia of all species of approximately 5 logs with fluences of ≥15 J cm<sup>−2</sup>
. Washing of the conidia before light exposure did not prevent photodynamic inactivation. Both NMBN and S137 accumulated in cytoplasmic structures, such as lipid bodies, of <named-content content-type="genus-species">C. acutatum</named-content>
 conidia. No damage to orange tree leaves was observed after APDT.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Appl. Environ. Microbiol</journal-id>
<journal-id journal-id-type="hwp">aem</journal-id>
<journal-id journal-id-type="pmc">aem</journal-id>
<journal-id journal-id-type="publisher-id">AEM</journal-id>
<journal-title-group><journal-title>Applied and Environmental Microbiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24362436</article-id>
<article-id pub-id-type="pmc">3957600</article-id>
<article-id pub-id-type="publisher-id">02788-13</article-id>
<article-id pub-id-type="doi">10.1128/AEM.02788-13</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Plant Microbiology</subject>
</subj-group>
</article-categories>
<title-group><article-title><italic>In Vitro</italic>
 Photodynamic Inactivation of Plant-Pathogenic Fungi <named-content content-type="genus-species">Colletotrichum acutatum</named-content>
 and <named-content content-type="genus-species">Colletotrichum gloeosporioides</named-content>
 with Novel Phenothiazinium Photosensitizers</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>de Menezes</surname>
<given-names>Henrique D.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rodrigues</surname>
<given-names>Gabriela B.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Teixeira</surname>
<given-names>Simone de Pádua</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Massola</surname>
<given-names>Nelson S.</given-names>
<suffix>Jr.</suffix>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bachmann</surname>
<given-names>Luciano</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wainwright</surname>
<given-names>Mark</given-names>
</name>
<xref ref-type="aff" rid="aff5"><sup>e</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Braga</surname>
<given-names>Gilberto U. L.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff6"><sup>f</sup>
</xref>
</contrib>
<aff id="aff1"><label>a</label>
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</aff>
<aff id="aff2"><label>b</label>
Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</aff>
<aff id="aff3"><label>c</label>
Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil</aff>
<aff id="aff4"><label>d</label>
Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</aff>
<aff id="aff5"><label>e</label>
School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom</aff>
<aff id="aff6"><label>f</label>
Research Support Center in Natural and Synthetic Products, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil</aff>
</contrib-group>
<author-notes><corresp id="cor1">Address correspondence to Gilberto U. L. Braga, <email>gbraga@fcfrp.usp.br</email>
.</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>3</month>
<year>2014</year>
</pub-date>
<volume>80</volume>
<issue>5</issue>
<fpage>1623</fpage>
<lpage>1632</lpage>
<history><date date-type="received"><day>19</day>
<month>8</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>18</day>
<month>12</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2014</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zam00514001623.pdf"></self-uri>
<abstract><p>The increasing tolerance to currently used fungicides in both clinical and agricultural areas is of great concern. The nonconventional light-based approach of antimicrobial photodynamic treatment (APDT) is a promising alternative to conventional fungicides. We evaluated the effects of APDT with four phenothiazinium derivatives (methylene blue [MB], new methylene blue N [NMBN], toluidine blue O [TBO], and the novel pentacyclic phenothiazinium photosensitizer [PS] S137) on conidia of three fungal species (<named-content content-type="genus-species">Colletotrichum acutatum</named-content>
, <named-content content-type="genus-species">Colletotrichum gloeosporioides</named-content>
, and <named-content content-type="genus-species">Aspergillus nidulans</named-content>
). The efficacy of APDT with each PS was determined, initially, based on photosensitizer MICs. Additionally, the effects of APDT with two selected PSs (NMBN and S137) on survival of conidia were evaluated. The subcellular localization of the PS in <named-content content-type="genus-species">C. acutatum</named-content>
 conidia was determined. The effects of photodynamic treatments on leaves of the plant host <named-content content-type="genus-species">Citrus sinensis</named-content>
 were also investigated. APDT with S137 showed the lowest MIC. MICs for S137 were 5 μM for the three fungal species when a fluence of 25 J cm<sup>−2</sup>
 was used. APDT with NMBN (50 μM) and S137 (10 μM) resulted in a reduction in the survival of the conidia of all species of approximately 5 logs with fluences of ≥15 J cm<sup>−2</sup>
. Washing of the conidia before light exposure did not prevent photodynamic inactivation. Both NMBN and S137 accumulated in cytoplasmic structures, such as lipid bodies, of <named-content content-type="genus-species">C. acutatum</named-content>
 conidia. No damage to orange tree leaves was observed after APDT.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>

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In Vitro Photodynamic Inactivation of Plant-Pathogenic Fungi Colletotrichum acutatum and Colletotrichum gloeosporioides with Novel Phenothiazinium Photosensitizers

In Vitro Photodynamic Inactivation of Plant-Pathogenic Fungi Colletotrichum acutatum and Colletotrichum gloeosporioides with Novel Phenothiazinium Photosensitizers

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Abstract

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