Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment</title><author><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Froelicher, Yann" sort="Froelicher, Yann" uniqKey="Froelicher Y" first="Yann" last="Froelicher">Yann Froelicher</name><affiliation><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Schwarz, Sergio" sort="Schwarz, Sergio" uniqKey="Schwarz S" first="Sergio" last="Schwarz">Sergio Schwarz</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Agusti, Manuel" sort="Agusti, Manuel" uniqKey="Agusti M" first="Manuel" last="Agustí">Manuel Agustí</name><affiliation><nlm:aff id="af3"><addr-line>Instituto Agroforestal Mediterráneo, Universidad Politécnica, Camino de Vera, s/n, 46022 Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Hernandez, Maria" sort="Hernandez, Maria" uniqKey="Hernandez M" first="María" last="Hernández">María Hernández</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Luro, Francois" sort="Luro, Francois" uniqKey="Luro F" first="François" last="Luro">François Luro</name><affiliation><nlm:aff id="af4"><addr-line>Unité GEQA, INRA, San Giuliano 20230 San Nicolao, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Morillon, Raphael" sort="Morillon, Raphael" uniqKey="Morillon R" first="Raphael" last="Morillon">Raphael Morillon</name><affiliation><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21586529</idno><idno type="pmc">3119611</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3119611</idno><idno type="RBID">PMC:3119611</idno><idno type="doi">10.1093/aob/mcr099</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000C22</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment</title><author><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Froelicher, Yann" sort="Froelicher, Yann" uniqKey="Froelicher Y" first="Yann" last="Froelicher">Yann Froelicher</name><affiliation><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Schwarz, Sergio" sort="Schwarz, Sergio" uniqKey="Schwarz S" first="Sergio" last="Schwarz">Sergio Schwarz</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Agusti, Manuel" sort="Agusti, Manuel" uniqKey="Agusti M" first="Manuel" last="Agustí">Manuel Agustí</name><affiliation><nlm:aff id="af3"><addr-line>Instituto Agroforestal Mediterráneo, Universidad Politécnica, Camino de Vera, s/n, 46022 Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Hernandez, Maria" sort="Hernandez, Maria" uniqKey="Hernandez M" first="María" last="Hernández">María Hernández</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Luro, Francois" sort="Luro, Francois" uniqKey="Luro F" first="François" last="Luro">François Luro</name><affiliation><nlm:aff id="af4"><addr-line>Unité GEQA, INRA, San Giuliano 20230 San Nicolao, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Morillon, Raphael" sort="Morillon, Raphael" uniqKey="Morillon R" first="Raphael" last="Morillon">Raphael Morillon</name><affiliation><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><affiliation><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">Annals of Botany</title><idno type="ISSN">0305-7364</idno><idno type="eISSN">1095-8290</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background and Aims</title><p>Polyploidy is a major component of plant evolution. The citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. The main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation.</p></sec><sec><title>Methods</title><p>Tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry and genotyped with 24 single sequence repeat (SSR) markers to analyse their genetic origin. Embryo rescue was used to grow all embryos contained in polyembryonic seeds of ‘Tardivo di Ciaculli’ mandarin, followed by characterization of the plantlets obtained by flow cytometry and SSR markers to accurately establish the rate of tetraploidization events and their potential tissue location. Inter-annual variations in tetraploid seedling rates were analysed for seven genotypes. Variation in tetraploid plantlet rates was analysed between different seedlings of the same genotype (‘Carrizo’ citrange; <italic>Citrus sinensis</italic> × <italic>Poncirus trifoliata</italic>) from seeds collected in different tropical, subtropical and Mediterranean countries.</p></sec><sec><title>Key Results</title><p>Tetraploid plants were obtained for all the studied diploid genotypes, except for four mandarins. All tetraploid plants were identical to their diploid maternal line for SSR markers and were not cytochimeric. Significant genotypic and environmental effects were observed, as well as negative correlation between mean temperature during the flowering period and tetraploidy seedling rates. The higher frequencies (20 %) of tetraploids were observed for citranges cultivated in the Mediterranean area.</p></sec><sec><title>Conclusions</title><p>Tetraploidization by chromosome doubling of nucellar cells are frequent events in apomictic citrus, and are affected by both genotypic and environmental factors. Colder conditions in marginal climatic areas appear to favour the expression of tetraploidization. Tetraploid genotypes arising from chromosome doubling of apomictic citrus are extensively being used as parents in breeding programmes to develop seedless triploid cultivars and have potential direct use as new rootstocks.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Ann Bot</journal-id><journal-id journal-id-type="publisher-id">annbot</journal-id><journal-id journal-id-type="hwp">annbot</journal-id><journal-title-group><journal-title>Annals of Botany</journal-title></journal-title-group><issn pub-type="ppub">0305-7364</issn><issn pub-type="epub">1095-8290</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21586529</article-id><article-id pub-id-type="pmc">3119611</article-id><article-id pub-id-type="doi">10.1093/aob/mcr099</article-id><article-id pub-id-type="publisher-id">mcr099</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject></subj-group></article-categories><title-group><article-title>Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Aleza</surname><given-names>Pablo</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Froelicher</surname><given-names>Yann</given-names></name><xref ref-type="aff" rid="af2">2</xref></contrib><contrib contrib-type="author"><name><surname>Schwarz</surname><given-names>Sergio</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Agustí</surname><given-names>Manuel</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Hernández</surname><given-names>María</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Juárez</surname><given-names>José</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Luro</surname><given-names>François</given-names></name><xref ref-type="aff" rid="af4">4</xref></contrib><contrib contrib-type="author"><name><surname>Morillon</surname><given-names>Raphael</given-names></name><xref ref-type="aff" rid="af2">2</xref></contrib><contrib contrib-type="author"><name><surname>Navarro</surname><given-names>Luis</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Ollitrault</surname><given-names>Patrick</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="aff" rid="af2">2</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="af1"><label>1</label><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></aff><aff id="af2"><label>2</label><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></aff><aff id="af3"><label>3</label><addr-line>Instituto Agroforestal Mediterráneo, Universidad Politécnica, Camino de Vera, s/n, 46022 Valencia, Spain</addr-line></aff><aff id="af4"><label>4</label><addr-line>Unité GEQA, INRA, San Giuliano 20230 San Nicolao, France</addr-line></aff><author-notes><corresp id="cor1"><label>*</label>For correspondence. E-mail <email>lnavarro@ivia.es</email> or <email>patrick.ollitrault@cirad.fr</email></corresp></author-notes><pub-date pub-type="ppub"><month>7</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>17</day><month>5</month><year>2011</year></pub-date><volume>108</volume><issue>1</issue><fpage>37</fpage><lpage>50</lpage><history><date date-type="received"><day>29</day><month>10</month><year>2010</year></date><date date-type="rev-request"><day>4</day><month>1</month><year>2011</year></date><date date-type="accepted"><day>7</day><month>3</month><year>2011</year></date></history><permissions><copyright-statement>© The Author 2011. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com</copyright-statement><copyright-year>2011</copyright-year></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="mcr099.pdf"></self-uri><abstract><sec><title>Background and Aims</title><p>Polyploidy is a major component of plant evolution. The citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. The main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation.</p></sec><sec><title>Methods</title><p>Tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry and genotyped with 24 single sequence repeat (SSR) markers to analyse their genetic origin. Embryo rescue was used to grow all embryos contained in polyembryonic seeds of ‘Tardivo di Ciaculli’ mandarin, followed by characterization of the plantlets obtained by flow cytometry and SSR markers to accurately establish the rate of tetraploidization events and their potential tissue location. Inter-annual variations in tetraploid seedling rates were analysed for seven genotypes. Variation in tetraploid plantlet rates was analysed between different seedlings of the same genotype (‘Carrizo’ citrange; <italic>Citrus sinensis</italic> × <italic>Poncirus trifoliata</italic>) from seeds collected in different tropical, subtropical and Mediterranean countries.</p></sec><sec><title>Key Results</title><p>Tetraploid plants were obtained for all the studied diploid genotypes, except for four mandarins. All tetraploid plants were identical to their diploid maternal line for SSR markers and were not cytochimeric. Significant genotypic and environmental effects were observed, as well as negative correlation between mean temperature during the flowering period and tetraploidy seedling rates. The higher frequencies (20 %) of tetraploids were observed for citranges cultivated in the Mediterranean area.</p></sec><sec><title>Conclusions</title><p>Tetraploidization by chromosome doubling of nucellar cells are frequent events in apomictic citrus, and are affected by both genotypic and environmental factors. Colder conditions in marginal climatic areas appear to favour the expression of tetraploidization. Tetraploid genotypes arising from chromosome doubling of apomictic citrus are extensively being used as parents in breeding programmes to develop seedless triploid cultivars and have potential direct use as new rootstocks.</p></sec></abstract><kwd-group><kwd><italic>Citrus</italic></kwd><kwd>polyembryony</kwd><kwd>apomixis</kwd><kwd>tetraploid</kwd><kwd>SSR markers</kwd><kwd>flow cytometry</kwd><kwd>chromosome doubling</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C22 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000C22 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.25. |  |