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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative Analysis of Expressed Genes from Cacao Meristems Infected by <italic>Moniliophthora perniciosa</italic></title><author><name sortKey="Gesteira, Abelmon S" sort="Gesteira, Abelmon S" uniqKey="Gesteira A" first="Abelmon S." last="Gesteira">Abelmon S. Gesteira</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Micheli, Fabienne" sort="Micheli, Fabienne" uniqKey="Micheli F" first="Fabienne" last="Micheli">Fabienne Micheli</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><institution>CIRAD-CP, UMR PIA</institution>,<addr-line>Montpellier</addr-line>,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Carels, Nicolas" sort="Carels, Nicolas" uniqKey="Carels N" first="Nicolas" last="Carels">Nicolas Carels</name><affiliation><nlm:aff id="af3"><institution>DCB/UESC, LABBI, Ilhéus</institution>,<addr-line>Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Da Silva, Aline C" sort="Da Silva, Aline C" uniqKey="Da Silva A" first="Aline C." last="Da Silva">Aline C. Da Silva</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Gramacho, Karina P" sort="Gramacho, Karina P" uniqKey="Gramacho K" first="Karina P." last="Gramacho">Karina P. Gramacho</name><affiliation><nlm:aff id="af4"><institution>CEPLAC/CEPEC, Cocoa Research Center</institution>,<addr-line>Itabuna, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Schuster, Ivan" sort="Schuster, Ivan" uniqKey="Schuster I" first="Ivan" last="Schuster">Ivan Schuster</name><affiliation><nlm:aff id="af5"><institution>COODETEC</institution>,<addr-line>Cascavel, Paraná</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Macedo, Joci N" sort="Macedo, Joci N" uniqKey="Macedo J" first="Joci N." last="Macêdo">Joci N. Macêdo</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Pereira, Goncalo A G" sort="Pereira, Goncalo A G" uniqKey="Pereira G" first="Gonçalo A. G." last="Pereira">Gonçalo A. G. Pereira</name><affiliation><nlm:aff id="af6"><institution>Universidade Estadual de Campinas, Instituto de Biologia</institution>,<addr-line>Departamento de Genética e Evolução-UNICAMP, Campinas, São Paulo</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Cascardo, Julio C M" sort="Cascardo, Julio C M" uniqKey="Cascardo J" first="Júlio C. M." last="Cascardo">Júlio C. M. Cascardo</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">17557832</idno><idno type="pmc">2735303</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2735303</idno><idno type="RBID">PMC:2735303</idno><idno type="doi">10.1093/aob/mcm092</idno><date when="2007">2007</date><idno type="wicri:Area/Pmc/Corpus">000C14</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comparative Analysis of Expressed Genes from Cacao Meristems Infected by <italic>Moniliophthora perniciosa</italic></title><author><name sortKey="Gesteira, Abelmon S" sort="Gesteira, Abelmon S" uniqKey="Gesteira A" first="Abelmon S." last="Gesteira">Abelmon S. Gesteira</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Micheli, Fabienne" sort="Micheli, Fabienne" uniqKey="Micheli F" first="Fabienne" last="Micheli">Fabienne Micheli</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><institution>CIRAD-CP, UMR PIA</institution>,<addr-line>Montpellier</addr-line>,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Carels, Nicolas" sort="Carels, Nicolas" uniqKey="Carels N" first="Nicolas" last="Carels">Nicolas Carels</name><affiliation><nlm:aff id="af3"><institution>DCB/UESC, LABBI, Ilhéus</institution>,<addr-line>Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Da Silva, Aline C" sort="Da Silva, Aline C" uniqKey="Da Silva A" first="Aline C." last="Da Silva">Aline C. Da Silva</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Gramacho, Karina P" sort="Gramacho, Karina P" uniqKey="Gramacho K" first="Karina P." last="Gramacho">Karina P. Gramacho</name><affiliation><nlm:aff id="af4"><institution>CEPLAC/CEPEC, Cocoa Research Center</institution>,<addr-line>Itabuna, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Schuster, Ivan" sort="Schuster, Ivan" uniqKey="Schuster I" first="Ivan" last="Schuster">Ivan Schuster</name><affiliation><nlm:aff id="af5"><institution>COODETEC</institution>,<addr-line>Cascavel, Paraná</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Macedo, Joci N" sort="Macedo, Joci N" uniqKey="Macedo J" first="Joci N." last="Macêdo">Joci N. Macêdo</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Pereira, Goncalo A G" sort="Pereira, Goncalo A G" uniqKey="Pereira G" first="Gonçalo A. G." last="Pereira">Gonçalo A. G. Pereira</name><affiliation><nlm:aff id="af6"><institution>Universidade Estadual de Campinas, Instituto de Biologia</institution>,<addr-line>Departamento de Genética e Evolução-UNICAMP, Campinas, São Paulo</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author><author><name sortKey="Cascardo, Julio C M" sort="Cascardo, Julio C M" uniqKey="Cascardo J" first="Júlio C. M." last="Cascardo">Júlio C. M. Cascardo</name><affiliation><nlm:aff id="af1"><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></nlm:aff></affiliation></author></analytic><series><title level="j">Annals of Botany</title><idno type="ISSN">0305-7364</idno><idno type="eISSN">1095-8290</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background and Aims</title><p>Witches' broom disease is caused by the hemibiotrophic basidiomycete <italic>Moniliophthora perniciosa</italic>, and is one of the most important diseases of cacao in the western hemisphere. Because very little is known about the global process of such disease development, expressed sequence tags (ESTs) were used to identify genes expressed during the <italic>Theobroma cacao</italic>–<italic>Moniliophthora perniciosa</italic> interaction.</p></sec><sec><title>Methods</title><p>Two cDNA libraries corresponding to the resistant (RT) and susceptible (SP) cacao–<italic>M. perniciosa</italic> interactions were constructed from total RNA, using the DB SMART Creator cDNA library kit (Clontech). Clones were randomly selected, sequenced from the 5′ end and analysed using bioinformatics tools including <italic>in silico</italic> analysis of the differential gene expression.</p></sec><sec><title>Key Results</title><p>A total of 6884 ESTs were generated from the RT and SP cDNA libraries. These ESTs were composed of 2585 singlets and 341 contigs for a total of 2926 non-redundant sequences. The redundancy of the libraries was low and their specificity high when compared with the few other cacao libraries already published. Sequence analysis allowed the assignment of a putative functional category for 54 % of sequences, whereas approx. 22 % of sequences corresponded to unknown function and approx. 24 % of sequences did not show any significant similarity with other proteins present in the database. Despite the similar overall distribution of the sequences in functional categories between the two libraries, qualitative differences were observed. Genes involved during the defence response to pathogen infection or in programmed cell death were identified, such as pathogenesis related-proteins, trypsin inhibitor or oxalate oxidase, and some of them showed an <italic>in silico</italic> differential expression between the resistant and the susceptible interactions.</p></sec><sec><title>Conclusions</title><p>As far as is known this is the first EST resource from the cacao–<italic>M. perniciosa</italic> interaction and it is believed that it will provide a significant contribution to the understanding of the molecular mechanisms of the resistance and susceptibility of cacao to <italic>M. perniciosa</italic>, to develop strategies to control witches broom, and as a source of polymorphism for molecular marker development and marker-assisted selection.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Ann Bot</journal-id><journal-id journal-id-type="publisher-id">Annals of Botany</journal-id><journal-title-group><journal-title>Annals of Botany</journal-title></journal-title-group><issn pub-type="ppub">0305-7364</issn><issn pub-type="epub">1095-8290</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">17557832</article-id><article-id pub-id-type="pmc">2735303</article-id><article-id pub-id-type="doi">10.1093/aob/mcm092</article-id><article-id pub-id-type="publisher-id">mcm092</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject></subj-group></article-categories><title-group><article-title>Comparative Analysis of Expressed Genes from Cacao Meristems Infected by <italic>Moniliophthora perniciosa</italic></article-title><alt-title alt-title-type="left-running"><italic>Gesteira</italic> et al. — <italic>ESTs from Witches' Broom Disease</italic></alt-title><alt-title alt-title-type="right-running">Gesteira <italic>et al</italic>. — ESTs from Witches' Broom Disease</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Gesteira</surname><given-names>Abelmon S.</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="author-notes" rid="FN1">†</xref></contrib><contrib contrib-type="author"><name><surname>Micheli</surname><given-names>Fabienne</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="aff" rid="af2">2</xref><xref ref-type="author-notes" rid="FN1">†</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Carels</surname><given-names>Nicolas</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Da Silva</surname><given-names>Aline C.</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="author-notes" rid="FN2">‡</xref></contrib><contrib contrib-type="author"><name><surname>Gramacho</surname><given-names>Karina P.</given-names></name><xref ref-type="aff" rid="af4">4</xref></contrib><contrib contrib-type="author"><name><surname>Schuster</surname><given-names>Ivan</given-names></name><xref ref-type="aff" rid="af5">5</xref></contrib><contrib contrib-type="author"><name><surname>Macêdo</surname><given-names>Joci N.</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="author-notes" rid="FN3">§</xref></contrib><contrib contrib-type="author"><name><surname>Pereira</surname><given-names>Gonçalo A. G.</given-names></name><xref ref-type="aff" rid="af6">6</xref></contrib><contrib contrib-type="author"><name><surname>Cascardo</surname><given-names>Júlio C. M.</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib></contrib-group><aff id="af1"><label>1</label><addr-line>DCB/UESC</addr-line>,<institution>Laboratório de Genômica e Expressão Gênica</institution>,<addr-line>Ilhéus, Bahia</addr-line>,<country>Brasil</country></aff><aff id="af2"><label>2</label><institution>CIRAD-CP, UMR PIA</institution>,<addr-line>Montpellier</addr-line>,<country>France</country></aff><aff id="af3"><label>3</label><institution>DCB/UESC, LABBI, Ilhéus</institution>,<addr-line>Bahia</addr-line>,<country>Brasil</country></aff><aff id="af4"><label>4</label><institution>CEPLAC/CEPEC, Cocoa Research Center</institution>,<addr-line>Itabuna, Bahia</addr-line>,<country>Brasil</country></aff><aff id="af5"><label>5</label><institution>COODETEC</institution>,<addr-line>Cascavel, Paraná</addr-line>,<country>Brasil</country></aff><aff id="af6"><label>6</label><institution>Universidade Estadual de Campinas, Instituto de Biologia</institution>,<addr-line>Departamento de Genética e Evolução-UNICAMP, Campinas, São Paulo</addr-line>,<country>Brasil</country></aff><author-notes><corresp id="cor1"><label>*</label>For correspondence. E-mail <email>fabienne.micheli@cirad.fr</email></corresp><fn id="FN1"><p>†These authors contributed equally to this work.</p></fn><fn id="FN2"><p>‡Present address: Centro de Pesquisas Gonçalo Moniz, Salvador, Bahia, Brasil.</p></fn><fn id="FN3"><p>§Present address: Centro de Biotecnologia Molecular Estrutural (CBME), Instituto de Fisica de Sao Carlos, São Carlos, São Paulo, Brasil.</p></fn></author-notes><pub-date pub-type="ppub"><month>7</month><year>2007</year></pub-date><pub-date pub-type="epub"><day>8</day><month>6</month><year>2007</year></pub-date><volume>100</volume><issue>1</issue><fpage>129</fpage><lpage>140</lpage><history><date date-type="received"><day>24</day><month>10</month><year>2006</year></date><date date-type="rev-request"><day>31</day><month>1</month><year>2007</year></date><date date-type="accepted"><day>21</day><month>3</month><year>2007</year></date></history><permissions><copyright-statement>© The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org</copyright-statement></permissions><abstract><sec><title>Background and Aims</title><p>Witches' broom disease is caused by the hemibiotrophic basidiomycete <italic>Moniliophthora perniciosa</italic>, and is one of the most important diseases of cacao in the western hemisphere. Because very little is known about the global process of such disease development, expressed sequence tags (ESTs) were used to identify genes expressed during the <italic>Theobroma cacao</italic>–<italic>Moniliophthora perniciosa</italic> interaction.</p></sec><sec><title>Methods</title><p>Two cDNA libraries corresponding to the resistant (RT) and susceptible (SP) cacao–<italic>M. perniciosa</italic> interactions were constructed from total RNA, using the DB SMART Creator cDNA library kit (Clontech). Clones were randomly selected, sequenced from the 5′ end and analysed using bioinformatics tools including <italic>in silico</italic> analysis of the differential gene expression.</p></sec><sec><title>Key Results</title><p>A total of 6884 ESTs were generated from the RT and SP cDNA libraries. These ESTs were composed of 2585 singlets and 341 contigs for a total of 2926 non-redundant sequences. The redundancy of the libraries was low and their specificity high when compared with the few other cacao libraries already published. Sequence analysis allowed the assignment of a putative functional category for 54 % of sequences, whereas approx. 22 % of sequences corresponded to unknown function and approx. 24 % of sequences did not show any significant similarity with other proteins present in the database. Despite the similar overall distribution of the sequences in functional categories between the two libraries, qualitative differences were observed. Genes involved during the defence response to pathogen infection or in programmed cell death were identified, such as pathogenesis related-proteins, trypsin inhibitor or oxalate oxidase, and some of them showed an <italic>in silico</italic> differential expression between the resistant and the susceptible interactions.</p></sec><sec><title>Conclusions</title><p>As far as is known this is the first EST resource from the cacao–<italic>M. perniciosa</italic> interaction and it is believed that it will provide a significant contribution to the understanding of the molecular mechanisms of the resistance and susceptibility of cacao to <italic>M. perniciosa</italic>, to develop strategies to control witches broom, and as a source of polymorphism for molecular marker development and marker-assisted selection.</p></sec></abstract><kwd-group><title>Key words</title><kwd><italic>Theobroma cacao</italic></kwd><kwd><italic>Moniliophthora perniciosa</italic></kwd><kwd>ESTs</kwd><kwd>resistance</kwd><kwd>programmed cell death</kwd><kwd>witches' broom disease</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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