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The Genome Sequence of an Isolate of Indian Citrus Ringspot Virus Infecting the Sweet Orange in India

Identifieur interne : 000A02 ( Pmc/Corpus ); précédent : 000A01; suivant : 000A03

The Genome Sequence of an Isolate of Indian Citrus Ringspot Virus Infecting the Sweet Orange in India

Auteurs : Prabha K ; V. K. Baranwal

Source :

RBID : PMC:3486449

Abstract

Whole-genome sequencing of an isolate of Mandarivirus infecting the sweet orange [Citrus sinensis (L) Blanco] in the western part of India (Pune) was done. The single-stranded positive-sense RNA genome of Indian citrus ringspot virus (ICRSV) Pune has 7,560 nucleotides (nt), excluding a poly(A) tail, comprised of 27.98% (2,115 nt) A, 32.12% (2,428 nt) C, 19.68% (1,488 nt) G, and 20.22% (1,529 nt) T residues. The genome, organized into six open reading frames (ORFs), shares 97.7% sequence identity with the complete genome of the ICRSV K1 isolate (AF406744.1) infecting the kinnow (Citrus reticulate Blanco, a hybrid between King and Willow mandarins) in north India. The ICRSV Pune genome formed a complex secondary structure with a large number of unpaired cytosine-rich regions, and recombination analysis highlighted potential recombination in the ICRSV genome.


Url:
DOI: 10.1128/JVI.02084-12
PubMed: 23087111
PubMed Central: 3486449

Links to Exploration step

PMC:3486449

Le document en format XML

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<p>Whole-genome sequencing of an isolate of
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infecting the sweet orange [
<named-content content-type="genus-species">Citrus sinensis</named-content>
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(ICRSV) Pune has 7,560 nucleotides (nt), excluding a poly(A) tail, comprised of 27.98% (2,115 nt) A, 32.12% (2,428 nt) C, 19.68% (1,488 nt) G, and 20.22% (1,529 nt) T residues. The genome, organized into six open reading frames (ORFs), shares 97.7% sequence identity with the complete genome of the ICRSV K1 isolate (AF406744.1) infecting the kinnow (
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Blanco, a hybrid between King and Willow mandarins) in north India. The ICRSV Pune genome formed a complex secondary structure with a large number of unpaired cytosine-rich regions, and recombination analysis highlighted potential recombination in the ICRSV genome.</p>
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<article-title>The Genome Sequence of an Isolate of Indian Citrus Ringspot Virus Infecting the Sweet Orange in India</article-title>
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<aff>Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India</aff>
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<corresp>Address correspondence to V. K. Baranwal,
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<month>11</month>
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<volume>86</volume>
<issue>22</issue>
<fpage>12446</fpage>
<lpage>12447</lpage>
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<date date-type="received">
<day>18</day>
<month>8</month>
<year>2012</year>
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<date date-type="accepted">
<day>22</day>
<month>8</month>
<year>2012</year>
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<p>Whole-genome sequencing of an isolate of
<named-content content-type="genus-species">Mandarivirus</named-content>
infecting the sweet orange [
<named-content content-type="genus-species">Citrus sinensis</named-content>
(L) Blanco] in the western part of India (Pune) was done. The single-stranded positive-sense RNA genome of
<named-content content-type="genus-species">Indian citrus ringspot virus</named-content>
(ICRSV) Pune has 7,560 nucleotides (nt), excluding a poly(A) tail, comprised of 27.98% (2,115 nt) A, 32.12% (2,428 nt) C, 19.68% (1,488 nt) G, and 20.22% (1,529 nt) T residues. The genome, organized into six open reading frames (ORFs), shares 97.7% sequence identity with the complete genome of the ICRSV K1 isolate (AF406744.1) infecting the kinnow (
<named-content content-type="genus-species">Citrus reticulate</named-content>
Blanco, a hybrid between King and Willow mandarins) in north India. The ICRSV Pune genome formed a complex secondary structure with a large number of unpaired cytosine-rich regions, and recombination analysis highlighted potential recombination in the ICRSV genome.</p>
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