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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Site of Fluoride Accumulation in Navel Orange Leaves <xref ref-type="fn" rid="fn1"><sup>1</sup></xref></title><author><name sortKey="Chang, Chong W" sort="Chang, Chong W" uniqKey="Chang C" first="Chong W." last="Chang">Chong W. Chang</name></author><author><name sortKey="Thompson, C Ray" sort="Thompson, C Ray" uniqKey="Thompson C" first="C. Ray" last="Thompson">C. Ray Thompson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">5908632</idno><idno type="pmc">1086321</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1086321</idno><idno type="RBID">PMC:1086321</idno><date when="1966">1966</date><idno type="wicri:Area/Pmc/Corpus">000935</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Site of Fluoride Accumulation in Navel Orange Leaves <xref ref-type="fn" rid="fn1"><sup>1</sup></xref></title><author><name sortKey="Chang, Chong W" sort="Chang, Chong W" uniqKey="Chang C" first="Chong W." last="Chang">Chong W. Chang</name></author><author><name sortKey="Thompson, C Ray" sort="Thompson, C Ray" uniqKey="Thompson C" first="C. Ray" last="Thompson">C. Ray Thompson</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="1966">1966</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Fluoride-polluted navel orange leaves, <italic>Citrus sinensis</italic> (Linn.) Osbeck, were fractionated into the subcellular components in hexane/carbon tetrachloride mixtures having various densities. Fluoride was determined at each fraction. Analyses were also made for the subcellular distribution of chlorophyll, nitrogen, and DNA to assess the extent of cross-contamination of each component.</p><p>The fraction containing cell wall, nuclei, and partly broken cells apparently contained a major amount of fluoride. However, if allowance was made for the cross-contamination of chloroplasts and chloroplast fragments, the fraction of chloroplasts was found to be the site of the highest fluoride accumulation. When each particulate component was washed with water after drying, the combined washings contained more than 50% of the total fluoride of the isolated fractions.</p><p>The usual method of subcellular fractionation with aqueous solvent shifted the major site of fluoride accumulation from the fraction of chloroplasts to that of the supernatant.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn></journal-meta><article-meta><article-id pub-id-type="pmid">5908632</article-id><article-id pub-id-type="pmc">1086321</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Site of Fluoride Accumulation in Navel Orange Leaves <xref ref-type="fn" rid="fn1"><sup>1</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Chang</surname><given-names>Chong W.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Thompson</surname><given-names>C. Ray</given-names></name></contrib></contrib-group><aff id="af1">Air Pollution Research Center, University of California, Riverside</aff><author-notes><fn id="fn1"><label>1</label><p> This investigation was supported by Public Health Service Research Grant AP 00052-02 from the Division of Air Pollution, Bureau of State Services, Public Health Service.</p></fn></author-notes><pub-date pub-type="ppub"><month>02</month><year>1966</year></pub-date><volume>41</volume><issue>2</issue><fpage>211</fpage><lpage>213</lpage><abstract><p>Fluoride-polluted navel orange leaves, <italic>Citrus sinensis</italic> (Linn.) Osbeck, were fractionated into the subcellular components in hexane/carbon tetrachloride mixtures having various densities. Fluoride was determined at each fraction. Analyses were also made for the subcellular distribution of chlorophyll, nitrogen, and DNA to assess the extent of cross-contamination of each component.</p><p>The fraction containing cell wall, nuclei, and partly broken cells apparently contained a major amount of fluoride. However, if allowance was made for the cross-contamination of chloroplasts and chloroplast fragments, the fraction of chloroplasts was found to be the site of the highest fluoride accumulation. When each particulate component was washed with water after drying, the combined washings contained more than 50% of the total fluoride of the isolated fractions.</p><p>The usual method of subcellular fractionation with aqueous solvent shifted the major site of fluoride accumulation from the fraction of chloroplasts to that of the supernatant.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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