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Lutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 Mutant[W][OA]

Identifieur interne : 000789 ( Pmc/Corpus ); précédent : 000788; suivant : 000790

Lutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 Mutant[W][OA]

Auteurs : Zhirong Li ; Tae Kyu Ahn ; Thomas J. Avenson ; Matteo Ballottari ; Jeffrey A. Cruz ; David M. Kramer ; Roberto Bassi ; Graham R. Fleming ; Jay D. Keasling ; Krishna K. Niyogi

Source :

RBID : PMC:2714924

Abstract

Plants protect themselves from excess absorbed light energy through thermal dissipation, which is measured as nonphotochemical quenching of chlorophyll fluorescence (NPQ). The major component of NPQ, qE, is induced by high transthylakoid ΔpH in excess light and depends on the xanthophyll cycle, in which violaxanthin and antheraxanthin are deepoxidized to form zeaxanthin. To investigate the xanthophyll dependence of qE, we identified suppressor of zeaxanthin-less1 (szl1) as a suppressor of the Arabidopsis thaliana npq1 mutant, which lacks zeaxanthin. szl1 npq1 plants have a partially restored qE but lack zeaxanthin and have low levels of violaxanthin, antheraxanthin, and neoxanthin. However, they accumulate more lutein and α-carotene than the wild type. szl1 contains a point mutation in the lycopene β-cyclase (LCYB) gene. Based on the pigment analysis, LCYB appears to be the major lycopene β-cyclase and is not involved in neoxanthin synthesis. The Lhcb4 (CP29) and Lhcb5 (CP26) protein levels are reduced by 50% in szl1 npq1 relative to the wild type, whereas other Lhcb proteins are present at wild-type levels. Analysis of carotenoid radical cation formation and leaf absorbance changes strongly suggest that the higher amount of lutein substitutes for zeaxanthin in qE, implying a direct role in qE, as well as a mechanism that is weakly sensitive to carotenoid structural properties.


Url:
DOI: 10.1105/tpc.109.066571
PubMed: 19549928
PubMed Central: 2714924

Links to Exploration step

PMC:2714924

Le document en format XML

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<title xml:lang="en">Lutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the
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<title xml:lang="en" level="a" type="main">Lutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the
<italic>Arabidopsis thaliana npq1</italic>
Mutant
<xref ref-type="fn" rid="fn2">[W]</xref>
<xref ref-type="fn" rid="fn3">[OA]</xref>
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<name sortKey="Li, Zhirong" sort="Li, Zhirong" uniqKey="Li Z" first="Zhirong" last="Li">Zhirong Li</name>
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<nlm:aff id="aff1">Department of Plant and Microbial Biology, University of California, Berkeley, California 94720-3102</nlm:aff>
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<affiliation>
<nlm:aff id="aff2">Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720</nlm:aff>
</affiliation>
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<author>
<name sortKey="Ahn, Tae Kyu" sort="Ahn, Tae Kyu" uniqKey="Ahn T" first="Tae Kyu" last="Ahn">Tae Kyu Ahn</name>
<affiliation>
<nlm:aff id="aff2">Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff3">Department of Chemistry, University of California, Berkeley, California 94720-1460</nlm:aff>
</affiliation>
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<name sortKey="Avenson, Thomas J" sort="Avenson, Thomas J" uniqKey="Avenson T" first="Thomas J." last="Avenson">Thomas J. Avenson</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant and Microbial Biology, University of California, Berkeley, California 94720-3102</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff3">Department of Chemistry, University of California, Berkeley, California 94720-1460</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Ballottari, Matteo" sort="Ballottari, Matteo" uniqKey="Ballottari M" first="Matteo" last="Ballottari">Matteo Ballottari</name>
<affiliation>
<nlm:aff id="aff4">Dipartimento Scientifico e Tecnologico, Università di Verona, I-37134 Verona, Italy</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Cruz, Jeffrey A" sort="Cruz, Jeffrey A" uniqKey="Cruz J" first="Jeffrey A." last="Cruz">Jeffrey A. Cruz</name>
<affiliation>
<nlm:aff id="aff5">Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340</nlm:aff>
</affiliation>
</author>
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<name sortKey="Kramer, David M" sort="Kramer, David M" uniqKey="Kramer D" first="David M." last="Kramer">David M. Kramer</name>
<affiliation>
<nlm:aff id="aff5">Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340</nlm:aff>
</affiliation>
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<author>
<name sortKey="Bassi, Roberto" sort="Bassi, Roberto" uniqKey="Bassi R" first="Roberto" last="Bassi">Roberto Bassi</name>
<affiliation>
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</affiliation>
</author>
<author>
<name sortKey="Fleming, Graham R" sort="Fleming, Graham R" uniqKey="Fleming G" first="Graham R." last="Fleming">Graham R. Fleming</name>
<affiliation>
<nlm:aff id="aff2">Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff3">Department of Chemistry, University of California, Berkeley, California 94720-1460</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Keasling, Jay D" sort="Keasling, Jay D" uniqKey="Keasling J" first="Jay D." last="Keasling">Jay D. Keasling</name>
<affiliation>
<nlm:aff id="aff2">Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff6">Department of Chemical Engineering, University of California, Berkeley, California 94720</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Niyogi, Krishna K" sort="Niyogi, Krishna K" uniqKey="Niyogi K" first="Krishna K." last="Niyogi">Krishna K. Niyogi</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant and Microbial Biology, University of California, Berkeley, California 94720-3102</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720</nlm:aff>
</affiliation>
</author>
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<series>
<title level="j">The Plant Cell</title>
<idno type="ISSN">1040-4651</idno>
<idno type="eISSN">1532-298X</idno>
<imprint>
<date when="2009">2009</date>
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<front>
<div type="abstract" xml:lang="en">
<p>Plants protect themselves from excess absorbed light energy through thermal dissipation, which is measured as nonphotochemical quenching of chlorophyll fluorescence (NPQ). The major component of NPQ, qE, is induced by high transthylakoid ΔpH in excess light and depends on the xanthophyll cycle, in which violaxanthin and antheraxanthin are deepoxidized to form zeaxanthin. To investigate the xanthophyll dependence of qE, we identified
<italic>suppressor of zeaxanthin-less1</italic>
(
<italic>szl1</italic>
) as a suppressor of the
<italic>Arabidopsis thaliana</italic>
<italic>npq1</italic>
mutant, which lacks zeaxanthin.
<italic>szl1 npq1</italic>
plants have a partially restored qE but lack zeaxanthin and have low levels of violaxanthin, antheraxanthin, and neoxanthin. However, they accumulate more lutein and α-carotene than the wild type.
<italic>szl1</italic>
contains a point mutation in the lycopene β-cyclase (
<italic>LCYB</italic>
) gene. Based on the pigment analysis, LCYB appears to be the major lycopene β-cyclase and is not involved in neoxanthin synthesis. The Lhcb4 (CP29) and Lhcb5 (CP26) protein levels are reduced by 50% in
<italic>szl1 npq1</italic>
relative to the wild type, whereas other Lhcb proteins are present at wild-type levels. Analysis of carotenoid radical cation formation and leaf absorbance changes strongly suggest that the higher amount of lutein substitutes for zeaxanthin in qE, implying a direct role in qE, as well as a mechanism that is weakly sensitive to carotenoid structural properties.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article">
<pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front>
<journal-meta>
<journal-id journal-id-type="nlm-ta">Plant Cell</journal-id>
<journal-id journal-id-type="publisher-id">plantcell</journal-id>
<journal-title>The Plant Cell</journal-title>
<issn pub-type="ppub">1040-4651</issn>
<issn pub-type="epub">1532-298X</issn>
<publisher>
<publisher-name>American Society of Plant Biologists</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="pmid">19549928</article-id>
<article-id pub-id-type="pmc">2714924</article-id>
<article-id pub-id-type="publisher-id">066571</article-id>
<article-id pub-id-type="doi">10.1105/tpc.109.066571</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Research Articles</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Lutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the
<italic>Arabidopsis thaliana npq1</italic>
Mutant
<xref ref-type="fn" rid="fn2">[W]</xref>
<xref ref-type="fn" rid="fn3">[OA]</xref>
</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Li</surname>
<given-names>Zhirong</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
<xref ref-type="aff" rid="aff2">b</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Ahn</surname>
<given-names>Tae Kyu</given-names>
</name>
<xref ref-type="aff" rid="aff2">b</xref>
<xref ref-type="aff" rid="aff3">c</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Avenson</surname>
<given-names>Thomas J.</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
<xref ref-type="aff" rid="aff3">c</xref>
<xref ref-type="fn" rid="fn1">1</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Ballottari</surname>
<given-names>Matteo</given-names>
</name>
<xref ref-type="aff" rid="aff4">d</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Cruz</surname>
<given-names>Jeffrey A.</given-names>
</name>
<xref ref-type="aff" rid="aff5">e</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Kramer</surname>
<given-names>David M.</given-names>
</name>
<xref ref-type="aff" rid="aff5">e</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Bassi</surname>
<given-names>Roberto</given-names>
</name>
<xref ref-type="aff" rid="aff4">d</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Fleming</surname>
<given-names>Graham R.</given-names>
</name>
<xref ref-type="aff" rid="aff2">b</xref>
<xref ref-type="aff" rid="aff3">c</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Keasling</surname>
<given-names>Jay D.</given-names>
</name>
<xref ref-type="aff" rid="aff2">b</xref>
<xref ref-type="aff" rid="aff6">f</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Niyogi</surname>
<given-names>Krishna K.</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
<xref ref-type="aff" rid="aff2">b</xref>
<xref ref-type="corresp" rid="cor1">2</xref>
</contrib>
</contrib-group>
<aff id="aff1">
<label>a</label>
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720-3102</aff>
<aff id="aff2">
<label>b</label>
Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720</aff>
<aff id="aff3">
<label>c</label>
Department of Chemistry, University of California, Berkeley, California 94720-1460</aff>
<aff id="aff4">
<label>d</label>
Dipartimento Scientifico e Tecnologico, Università di Verona, I-37134 Verona, Italy</aff>
<aff id="aff5">
<label>e</label>
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340</aff>
<aff id="aff6">
<label>f</label>
Department of Chemical Engineering, University of California, Berkeley, California 94720</aff>
<author-notes>
<fn id="fn1">
<label>1</label>
<p>Current address: Monsanto, 62 Maritime Drive, Mystic, CT 06355.</p>
</fn>
<fn id="cor1">
<label>2</label>
<p>Address correspondence to
<email>niyogi@nature.berkeley.edu</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub">
<month>6</month>
<year>2009</year>
</pub-date>
<volume>21</volume>
<issue>6</issue>
<fpage>1798</fpage>
<lpage>1812</lpage>
<history>
<date date-type="received">
<day>25</day>
<month>2</month>
<year>2009</year>
</date>
<date date-type="rev-recd">
<day>6</day>
<month>5</month>
<year>2009</year>
</date>
<date date-type="accepted">
<day>4</day>
<month>6</month>
<year>2009</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright © 2009, American Society of Plant Biologists</copyright-statement>
</permissions>
<abstract>
<p>Plants protect themselves from excess absorbed light energy through thermal dissipation, which is measured as nonphotochemical quenching of chlorophyll fluorescence (NPQ). The major component of NPQ, qE, is induced by high transthylakoid ΔpH in excess light and depends on the xanthophyll cycle, in which violaxanthin and antheraxanthin are deepoxidized to form zeaxanthin. To investigate the xanthophyll dependence of qE, we identified
<italic>suppressor of zeaxanthin-less1</italic>
(
<italic>szl1</italic>
) as a suppressor of the
<italic>Arabidopsis thaliana</italic>
<italic>npq1</italic>
mutant, which lacks zeaxanthin.
<italic>szl1 npq1</italic>
plants have a partially restored qE but lack zeaxanthin and have low levels of violaxanthin, antheraxanthin, and neoxanthin. However, they accumulate more lutein and α-carotene than the wild type.
<italic>szl1</italic>
contains a point mutation in the lycopene β-cyclase (
<italic>LCYB</italic>
) gene. Based on the pigment analysis, LCYB appears to be the major lycopene β-cyclase and is not involved in neoxanthin synthesis. The Lhcb4 (CP29) and Lhcb5 (CP26) protein levels are reduced by 50% in
<italic>szl1 npq1</italic>
relative to the wild type, whereas other Lhcb proteins are present at wild-type levels. Analysis of carotenoid radical cation formation and leaf absorbance changes strongly suggest that the higher amount of lutein substitutes for zeaxanthin in qE, implying a direct role in qE, as well as a mechanism that is weakly sensitive to carotenoid structural properties.</p>
</abstract>
</article-meta>
<notes>
<fn-group>
<fn>
<p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (
<ext-link ext-link-type="uri" xlink:href="www.plantcell.org">www.plantcell.org</ext-link>
) is: Krishna K. Niyogi (
<email>niyogi@nature.berkeley.edu</email>
).</p>
</fn>
<fn id="fn2">
<label>[W]</label>
<p>Online version contains Web-only data.</p>
</fn>
<fn id="fn3">
<label>[OA]</label>
<p>Open access articles can be viewed online without a subscription.</p>
</fn>
<fn>
<p>
<ext-link ext-link-type="uri" xlink:href="www.plantcell.org/cgi/doi/10.1105/tpc.109.066571">www.plantcell.org/cgi/doi/10.1105/tpc.109.066571</ext-link>
</p>
</fn>
</fn-group>
</notes>
</front>
</pmc>
</record>

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