OrangerV1, Pmc, Corpus, bibRecord, 000786

Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of MethylketonesW⃞

Identifieur interne : 000786 ( Pmc/Corpus ); précédent : 000785; suivant : 000787

Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of MethylketonesW⃞

Auteurs : Eyal Fridman ; Jihong Wang ; Yoko Iijima ; John E. Froehlich ; David R. Gang ; John Ohlrogge ; Eran Pichersky

Source :

The Plant Cell [ 1040-4651 ] ; 2005.

RBID : PMC:1088000

Abstract

Medium-length methylketones (C₇-C₁₅) are highly effective in protecting plants from numerous pests. We used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato Lycopersicon hirsutum f glabratum (accession PI126449). A comparison of gland EST databases from accession PI126449 and a second L. hirsutum accession, LA1777, whose glands do not contain methylketones, showed that the expression of genes for fatty acid biosynthesis is elevated in PI126449 glands, suggesting de novo biosynthesis of methylketones. A cDNA abundant in the PI126449 gland EST database but rare in the LA1777 database was similar in sequence to plant esterases. This cDNA, designated Methylketone Synthase 1 (MKS1), was expressed in Escherichia coli and the purified protein used to catalyze in vitro reactions in which C₁₂, C₁₄, and C₁₆ β-ketoacyl–acyl-carrier-proteins (intermediates in fatty acid biosynthesis) were hydrolyzed and decarboxylated to give C₁₁, C₁₃, and C₁₅ methylketones, respectively. Although MKS1 does not contain a classical transit peptide, in vitro import assays showed that it was targeted to the stroma of plastids, where fatty acid biosynthesis occurs. Levels of MKS1 transcript, protein, and enzymatic activity were correlated with levels of methylketones and gland density in a variety of tomato accessions and in different plant organs.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1088000

DOI: 10.1105/tpc.104.029736
PubMed: 15772286
PubMed Central: 1088000

Links to Exploration step

PMC:1088000

Le document en format XML

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 Identify a Key Enzyme in the Biosynthesis of Methylketones<xref ref-type="fn" rid="fn2">W⃞</xref>
</title>
<author><name sortKey="Fridman, Eyal" sort="Fridman, Eyal" uniqKey="Fridman E" first="Eyal" last="Fridman">Eyal Fridman</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Jihong" sort="Wang, Jihong" uniqKey="Wang J" first="Jihong" last="Wang">Jihong Wang</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Iijima, Yoko" sort="Iijima, Yoko" uniqKey="Iijima Y" first="Yoko" last="Iijima">Yoko Iijima</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Froehlich, John E" sort="Froehlich, John E" uniqKey="Froehlich J" first="John E." last="Froehlich">John E. Froehlich</name>
<affiliation><nlm:aff id="aff2">Michigan State University, Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gang, David R" sort="Gang, David R" uniqKey="Gang D" first="David R." last="Gang">David R. Gang</name>
<affiliation><nlm:aff id="aff3">Department of Plant Biology, University of Arizona, Tuscon, Arizona 85721-0036</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ohlrogge, John" sort="Ohlrogge, John" uniqKey="Ohlrogge J" first="John" last="Ohlrogge">John Ohlrogge</name>
<affiliation><nlm:aff id="aff4">Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pichersky, Eran" sort="Pichersky, Eran" uniqKey="Pichersky E" first="Eran" last="Pichersky">Eran Pichersky</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
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<idno type="pmc">1088000</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1088000</idno>
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<idno type="doi">10.1105/tpc.104.029736</idno>
<date when="2005">2005</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species <italic>Lycopersicon hirsutum</italic>
 Identify a Key Enzyme in the Biosynthesis of Methylketones<xref ref-type="fn" rid="fn2">W⃞</xref>
</title>
<author><name sortKey="Fridman, Eyal" sort="Fridman, Eyal" uniqKey="Fridman E" first="Eyal" last="Fridman">Eyal Fridman</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Jihong" sort="Wang, Jihong" uniqKey="Wang J" first="Jihong" last="Wang">Jihong Wang</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Iijima, Yoko" sort="Iijima, Yoko" uniqKey="Iijima Y" first="Yoko" last="Iijima">Yoko Iijima</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Froehlich, John E" sort="Froehlich, John E" uniqKey="Froehlich J" first="John E." last="Froehlich">John E. Froehlich</name>
<affiliation><nlm:aff id="aff2">Michigan State University, Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gang, David R" sort="Gang, David R" uniqKey="Gang D" first="David R." last="Gang">David R. Gang</name>
<affiliation><nlm:aff id="aff3">Department of Plant Biology, University of Arizona, Tuscon, Arizona 85721-0036</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ohlrogge, John" sort="Ohlrogge, John" uniqKey="Ohlrogge J" first="John" last="Ohlrogge">John Ohlrogge</name>
<affiliation><nlm:aff id="aff4">Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pichersky, Eran" sort="Pichersky, Eran" uniqKey="Pichersky E" first="Eran" last="Pichersky">Eran Pichersky</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">The Plant Cell</title>
<idno type="ISSN">1040-4651</idno>
<idno type="eISSN">1532-298X</idno>
<imprint><date when="2005">2005</date>
</imprint>
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<front><div type="abstract" xml:lang="en"><p>Medium-length methylketones (C<sub>7</sub>
-C<sub>15</sub>
) are highly effective in protecting plants from numerous pests. We used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato <italic>Lycopersicon hirsutum</italic>
 f <italic>glabratum</italic>
 (accession <ext-link ext-link-type="gen" xlink:href="PI126449">PI126449</ext-link>
). A comparison of gland EST databases from accession <ext-link ext-link-type="gen" xlink:href="PI126449">PI126449</ext-link>
 and a second <italic>L. hirsutum</italic>
 accession, <ext-link ext-link-type="gen" xlink:href="LA1777">LA1777</ext-link>
, whose glands do not contain methylketones, showed that the expression of genes for fatty acid biosynthesis is elevated in PI126449 glands, suggesting de novo biosynthesis of methylketones. A cDNA abundant in the PI126449 gland EST database but rare in the LA1777 database was similar in sequence to plant esterases. This cDNA, designated <italic>Methylketone Synthase 1</italic>
 (<italic>MKS1</italic>
), was expressed in <italic>Escherichia coli</italic>
 and the purified protein used to catalyze in vitro reactions in which C<sub>12</sub>
, C<sub>14</sub>
, and C<sub>16</sub>
 β-ketoacyl–acyl-carrier-proteins (intermediates in fatty acid biosynthesis) were hydrolyzed and decarboxylated to give C<sub>11</sub>
, C<sub>13</sub>
, and C<sub>15</sub>
 methylketones, respectively. Although MKS1 does not contain a classical transit peptide, in vitro import assays showed that it was targeted to the stroma of plastids, where fatty acid biosynthesis occurs. Levels of <italic>MKS1</italic>
 transcript, protein, and enzymatic activity were correlated with levels of methylketones and gland density in a variety of tomato accessions and in different plant organs.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell</journal-id>
<journal-id journal-id-type="publisher-id">plantcell</journal-id>
<journal-title>The Plant Cell</journal-title>
<issn pub-type="ppub">1040-4651</issn>
<issn pub-type="epub">1532-298X</issn>
<publisher><publisher-name>American Society of Plant Biologists</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">15772286</article-id>
<article-id pub-id-type="pmc">1088000</article-id>
<article-id pub-id-type="publisher-id">029736</article-id>
<article-id pub-id-type="doi">10.1105/tpc.104.029736</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species <italic>Lycopersicon hirsutum</italic>
 Identify a Key Enzyme in the Biosynthesis of Methylketones<xref ref-type="fn" rid="fn2">W⃞</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Fridman</surname>
<given-names>Eyal</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Jihong</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
<xref ref-type="fn" rid="fn1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Iijima</surname>
<given-names>Yoko</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Froehlich</surname>
<given-names>John E.</given-names>
</name>
<xref ref-type="aff" rid="aff2">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gang</surname>
<given-names>David R.</given-names>
</name>
<xref ref-type="aff" rid="aff3">c</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ohlrogge</surname>
<given-names>John</given-names>
</name>
<xref ref-type="aff" rid="aff4">d</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pichersky</surname>
<given-names>Eran</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
<xref ref-type="corresp" rid="cor1">2</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>a</label>
Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</aff>
<aff id="aff2"><label>b</label>
Michigan State University, Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824</aff>
<aff id="aff3"><label>c</label>
Department of Plant Biology, University of Arizona, Tuscon, Arizona 85721-0036</aff>
<aff id="aff4"><label>d</label>
Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824</aff>
<author-notes><fn id="fn1"><label>1</label>
<p>Current address: Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.</p>
</fn>
<fn id="cor1"><label>2</label>
<p>To whom correspondence should be addressed. E-mail <email>lelx@umich.edu</email>
; fax 734-936-3522.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>4</month>
<year>2005</year>
</pub-date>
<volume>17</volume>
<issue>4</issue>
<fpage>1252</fpage>
<lpage>1267</lpage>
<history><date date-type="received"><day>27</day>
<month>11</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>12</day>
<month>2</month>
<year>2005</year>
</date>
</history>
<copyright-statement>Copyright © 2005, American Society of Plant Biologists</copyright-statement>
<copyright-year>2005</copyright-year>
<abstract><p>Medium-length methylketones (C<sub>7</sub>
-C<sub>15</sub>
) are highly effective in protecting plants from numerous pests. We used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato <italic>Lycopersicon hirsutum</italic>
 f <italic>glabratum</italic>
 (accession <ext-link ext-link-type="gen" xlink:href="PI126449">PI126449</ext-link>
). A comparison of gland EST databases from accession <ext-link ext-link-type="gen" xlink:href="PI126449">PI126449</ext-link>
 and a second <italic>L. hirsutum</italic>
 accession, <ext-link ext-link-type="gen" xlink:href="LA1777">LA1777</ext-link>
, whose glands do not contain methylketones, showed that the expression of genes for fatty acid biosynthesis is elevated in PI126449 glands, suggesting de novo biosynthesis of methylketones. A cDNA abundant in the PI126449 gland EST database but rare in the LA1777 database was similar in sequence to plant esterases. This cDNA, designated <italic>Methylketone Synthase 1</italic>
 (<italic>MKS1</italic>
), was expressed in <italic>Escherichia coli</italic>
 and the purified protein used to catalyze in vitro reactions in which C<sub>12</sub>
, C<sub>14</sub>
, and C<sub>16</sub>
 β-ketoacyl–acyl-carrier-proteins (intermediates in fatty acid biosynthesis) were hydrolyzed and decarboxylated to give C<sub>11</sub>
, C<sub>13</sub>
, and C<sub>15</sub>
 methylketones, respectively. Although MKS1 does not contain a classical transit peptide, in vitro import assays showed that it was targeted to the stroma of plastids, where fatty acid biosynthesis occurs. Levels of <italic>MKS1</italic>
 transcript, protein, and enzymatic activity were correlated with levels of methylketones and gland density in a variety of tomato accessions and in different plant organs.</p>
</abstract>
</article-meta>
<notes><fn-group><fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantcell.org">www.plantcell.org</ext-link>
) is: Eran Pichersky (<email>lelx@umich.edu</email>
).</p>
</fn>
<fn id="fn2"><label>W⃞</label>
<p>Online version contains Web-only data.</p>
</fn>
<fn><p>Article, publication date, and citation information can be found at <ext-link ext-link-type="uri" xlink:href="www.plantcell.org/cgi/doi/10.1105/tpc.104.029736">www.plantcell.org/cgi/doi/10.1105/tpc.104.029736</ext-link>
.</p>
</fn>
</fn-group>
</notes>
</front>
</pmc>
</record>

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Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of MethylketonesW⃞

Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of MethylketonesW⃞

Source :

Abstract

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