Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of MethylketonesW⃞
Identifieur interne : 000786 ( Pmc/Corpus ); précédent : 000785; suivant : 000787Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of MethylketonesW⃞
Auteurs : Eyal Fridman ; Jihong Wang ; Yoko Iijima ; John E. Froehlich ; David R. Gang ; John Ohlrogge ; Eran PicherskySource :
- The Plant Cell [ 1040-4651 ] ; 2005.
Abstract
Medium-length methylketones (C7-C15) are highly effective in protecting plants from numerous pests. We used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato
Url:
DOI: 10.1105/tpc.104.029736
PubMed: 15772286
PubMed Central: 1088000
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PMC:1088000Le document en format XML
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Identify a Key Enzyme in the Biosynthesis of Methylketones<xref ref-type="fn" rid="fn2">W⃞</xref>
</title>
<author><name sortKey="Fridman, Eyal" sort="Fridman, Eyal" uniqKey="Fridman E" first="Eyal" last="Fridman">Eyal Fridman</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Jihong" sort="Wang, Jihong" uniqKey="Wang J" first="Jihong" last="Wang">Jihong Wang</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Iijima, Yoko" sort="Iijima, Yoko" uniqKey="Iijima Y" first="Yoko" last="Iijima">Yoko Iijima</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Froehlich, John E" sort="Froehlich, John E" uniqKey="Froehlich J" first="John E." last="Froehlich">John E. Froehlich</name>
<affiliation><nlm:aff id="aff2">Michigan State University, Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gang, David R" sort="Gang, David R" uniqKey="Gang D" first="David R." last="Gang">David R. Gang</name>
<affiliation><nlm:aff id="aff3">Department of Plant Biology, University of Arizona, Tuscon, Arizona 85721-0036</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ohlrogge, John" sort="Ohlrogge, John" uniqKey="Ohlrogge J" first="John" last="Ohlrogge">John Ohlrogge</name>
<affiliation><nlm:aff id="aff4">Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pichersky, Eran" sort="Pichersky, Eran" uniqKey="Pichersky E" first="Eran" last="Pichersky">Eran Pichersky</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
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<idno type="pmid">15772286</idno>
<idno type="pmc">1088000</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1088000</idno>
<idno type="RBID">PMC:1088000</idno>
<idno type="doi">10.1105/tpc.104.029736</idno>
<date when="2005">2005</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species <italic>Lycopersicon hirsutum</italic>
Identify a Key Enzyme in the Biosynthesis of Methylketones<xref ref-type="fn" rid="fn2">W⃞</xref>
</title>
<author><name sortKey="Fridman, Eyal" sort="Fridman, Eyal" uniqKey="Fridman E" first="Eyal" last="Fridman">Eyal Fridman</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Jihong" sort="Wang, Jihong" uniqKey="Wang J" first="Jihong" last="Wang">Jihong Wang</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Iijima, Yoko" sort="Iijima, Yoko" uniqKey="Iijima Y" first="Yoko" last="Iijima">Yoko Iijima</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Froehlich, John E" sort="Froehlich, John E" uniqKey="Froehlich J" first="John E." last="Froehlich">John E. Froehlich</name>
<affiliation><nlm:aff id="aff2">Michigan State University, Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gang, David R" sort="Gang, David R" uniqKey="Gang D" first="David R." last="Gang">David R. Gang</name>
<affiliation><nlm:aff id="aff3">Department of Plant Biology, University of Arizona, Tuscon, Arizona 85721-0036</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ohlrogge, John" sort="Ohlrogge, John" uniqKey="Ohlrogge J" first="John" last="Ohlrogge">John Ohlrogge</name>
<affiliation><nlm:aff id="aff4">Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pichersky, Eran" sort="Pichersky, Eran" uniqKey="Pichersky E" first="Eran" last="Pichersky">Eran Pichersky</name>
<affiliation><nlm:aff id="aff1">Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">The Plant Cell</title>
<idno type="ISSN">1040-4651</idno>
<idno type="eISSN">1532-298X</idno>
<imprint><date when="2005">2005</date>
</imprint>
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<front><div type="abstract" xml:lang="en"><p>Medium-length methylketones (C<sub>7</sub>
-C<sub>15</sub>
) are highly effective in protecting plants from numerous pests. We used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato <italic>Lycopersicon hirsutum</italic>
f <italic>glabratum</italic>
(accession <ext-link ext-link-type="gen" xlink:href="PI126449">PI126449</ext-link>
). A comparison of gland EST databases from accession <ext-link ext-link-type="gen" xlink:href="PI126449">PI126449</ext-link>
and a second <italic>L. hirsutum</italic>
accession, <ext-link ext-link-type="gen" xlink:href="LA1777">LA1777</ext-link>
, whose glands do not contain methylketones, showed that the expression of genes for fatty acid biosynthesis is elevated in PI126449 glands, suggesting de novo biosynthesis of methylketones. A cDNA abundant in the PI126449 gland EST database but rare in the LA1777 database was similar in sequence to plant esterases. This cDNA, designated <italic>Methylketone Synthase 1</italic>
(<italic>MKS1</italic>
), was expressed in <italic>Escherichia coli</italic>
and the purified protein used to catalyze in vitro reactions in which C<sub>12</sub>
, C<sub>14</sub>
, and C<sub>16</sub>
β-ketoacyl–acyl-carrier-proteins (intermediates in fatty acid biosynthesis) were hydrolyzed and decarboxylated to give C<sub>11</sub>
, C<sub>13</sub>
, and C<sub>15</sub>
methylketones, respectively. Although MKS1 does not contain a classical transit peptide, in vitro import assays showed that it was targeted to the stroma of plastids, where fatty acid biosynthesis occurs. Levels of <italic>MKS1</italic>
transcript, protein, and enzymatic activity were correlated with levels of methylketones and gland density in a variety of tomato accessions and in different plant organs.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell</journal-id>
<journal-id journal-id-type="publisher-id">plantcell</journal-id>
<journal-title>The Plant Cell</journal-title>
<issn pub-type="ppub">1040-4651</issn>
<issn pub-type="epub">1532-298X</issn>
<publisher><publisher-name>American Society of Plant Biologists</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">15772286</article-id>
<article-id pub-id-type="pmc">1088000</article-id>
<article-id pub-id-type="publisher-id">029736</article-id>
<article-id pub-id-type="doi">10.1105/tpc.104.029736</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species <italic>Lycopersicon hirsutum</italic>
Identify a Key Enzyme in the Biosynthesis of Methylketones<xref ref-type="fn" rid="fn2">W⃞</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Fridman</surname>
<given-names>Eyal</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Jihong</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
<xref ref-type="fn" rid="fn1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Iijima</surname>
<given-names>Yoko</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Froehlich</surname>
<given-names>John E.</given-names>
</name>
<xref ref-type="aff" rid="aff2">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gang</surname>
<given-names>David R.</given-names>
</name>
<xref ref-type="aff" rid="aff3">c</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ohlrogge</surname>
<given-names>John</given-names>
</name>
<xref ref-type="aff" rid="aff4">d</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pichersky</surname>
<given-names>Eran</given-names>
</name>
<xref ref-type="aff" rid="aff1">a</xref>
<xref ref-type="corresp" rid="cor1">2</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>a</label>
Department of Molecular, Cellular, and Developmental Biology, University of Michigan Ann Arbor, Michigan 48109-1048</aff>
<aff id="aff2"><label>b</label>
Michigan State University, Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824</aff>
<aff id="aff3"><label>c</label>
Department of Plant Biology, University of Arizona, Tuscon, Arizona 85721-0036</aff>
<aff id="aff4"><label>d</label>
Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824</aff>
<author-notes><fn id="fn1"><label>1</label>
<p>Current address: Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.</p>
</fn>
<fn id="cor1"><label>2</label>
<p>To whom correspondence should be addressed. E-mail <email>lelx@umich.edu</email>
; fax 734-936-3522.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>4</month>
<year>2005</year>
</pub-date>
<volume>17</volume>
<issue>4</issue>
<fpage>1252</fpage>
<lpage>1267</lpage>
<history><date date-type="received"><day>27</day>
<month>11</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>12</day>
<month>2</month>
<year>2005</year>
</date>
</history>
<copyright-statement>Copyright © 2005, American Society of Plant Biologists</copyright-statement>
<copyright-year>2005</copyright-year>
<abstract><p>Medium-length methylketones (C<sub>7</sub>
-C<sub>15</sub>
) are highly effective in protecting plants from numerous pests. We used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato <italic>Lycopersicon hirsutum</italic>
f <italic>glabratum</italic>
(accession <ext-link ext-link-type="gen" xlink:href="PI126449">PI126449</ext-link>
). A comparison of gland EST databases from accession <ext-link ext-link-type="gen" xlink:href="PI126449">PI126449</ext-link>
and a second <italic>L. hirsutum</italic>
accession, <ext-link ext-link-type="gen" xlink:href="LA1777">LA1777</ext-link>
, whose glands do not contain methylketones, showed that the expression of genes for fatty acid biosynthesis is elevated in PI126449 glands, suggesting de novo biosynthesis of methylketones. A cDNA abundant in the PI126449 gland EST database but rare in the LA1777 database was similar in sequence to plant esterases. This cDNA, designated <italic>Methylketone Synthase 1</italic>
(<italic>MKS1</italic>
), was expressed in <italic>Escherichia coli</italic>
and the purified protein used to catalyze in vitro reactions in which C<sub>12</sub>
, C<sub>14</sub>
, and C<sub>16</sub>
β-ketoacyl–acyl-carrier-proteins (intermediates in fatty acid biosynthesis) were hydrolyzed and decarboxylated to give C<sub>11</sub>
, C<sub>13</sub>
, and C<sub>15</sub>
methylketones, respectively. Although MKS1 does not contain a classical transit peptide, in vitro import assays showed that it was targeted to the stroma of plastids, where fatty acid biosynthesis occurs. Levels of <italic>MKS1</italic>
transcript, protein, and enzymatic activity were correlated with levels of methylketones and gland density in a variety of tomato accessions and in different plant organs.</p>
</abstract>
</article-meta>
<notes><fn-group><fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantcell.org">www.plantcell.org</ext-link>
) is: Eran Pichersky (<email>lelx@umich.edu</email>
).</p>
</fn>
<fn id="fn2"><label>W⃞</label>
<p>Online version contains Web-only data.</p>
</fn>
<fn><p>Article, publication date, and citation information can be found at <ext-link ext-link-type="uri" xlink:href="www.plantcell.org/cgi/doi/10.1105/tpc.104.029736">www.plantcell.org/cgi/doi/10.1105/tpc.104.029736</ext-link>
.</p>
</fn>
</fn-group>
</notes>
</front>
</pmc>
</record>
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