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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport</title><author><name sortKey="Mathews, Helena" sort="Mathews, Helena" uniqKey="Mathews H" first="Helena" last="Mathews">Helena Mathews</name></author><author><name sortKey="Clendennen, Stephanie K" sort="Clendennen, Stephanie K" uniqKey="Clendennen S" first="Stephanie K." last="Clendennen">Stephanie K. Clendennen</name></author><author><name sortKey="Caldwell, Colby G" sort="Caldwell, Colby G" uniqKey="Caldwell C" first="Colby G." last="Caldwell">Colby G. Caldwell</name></author><author><name sortKey="Liu, Xing Liang" sort="Liu, Xing Liang" uniqKey="Liu X" first="Xing Liang" last="Liu">Xing Liang Liu</name></author><author><name sortKey="Connors, Karin" sort="Connors, Karin" uniqKey="Connors K" first="Karin" last="Connors">Karin Connors</name></author><author><name sortKey="Matheis, Nikolaus" sort="Matheis, Nikolaus" uniqKey="Matheis N" first="Nikolaus" last="Matheis">Nikolaus Matheis</name></author><author><name sortKey="Schuster, Debra K" sort="Schuster, Debra K" uniqKey="Schuster D" first="Debra K." last="Schuster">Debra K. Schuster</name></author><author><name sortKey="Menasco, D J" sort="Menasco, D J" uniqKey="Menasco D" first="D. J." last="Menasco">D. J. Menasco</name></author><author><name sortKey="Wagoner, Wendy" sort="Wagoner, Wendy" uniqKey="Wagoner W" first="Wendy" last="Wagoner">Wendy Wagoner</name></author><author><name sortKey="Lightner, Jonathan" sort="Lightner, Jonathan" uniqKey="Lightner J" first="Jonathan" last="Lightner">Jonathan Lightner</name></author><author><name sortKey="Wagner, D Ry" sort="Wagner, D Ry" uniqKey="Wagner D" first="D. Ry" last="Wagner">D. Ry Wagner</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12897245</idno><idno type="pmc">167162</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC167162</idno><idno type="RBID">PMC:167162</idno><idno type="doi">10.1105/tpc.012963</idno><date when="2003">2003</date><idno type="wicri:Area/Pmc/Corpus">000781</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport</title><author><name sortKey="Mathews, Helena" sort="Mathews, Helena" uniqKey="Mathews H" first="Helena" last="Mathews">Helena Mathews</name></author><author><name sortKey="Clendennen, Stephanie K" sort="Clendennen, Stephanie K" uniqKey="Clendennen S" first="Stephanie K." last="Clendennen">Stephanie K. Clendennen</name></author><author><name sortKey="Caldwell, Colby G" sort="Caldwell, Colby G" uniqKey="Caldwell C" first="Colby G." last="Caldwell">Colby G. Caldwell</name></author><author><name sortKey="Liu, Xing Liang" sort="Liu, Xing Liang" uniqKey="Liu X" first="Xing Liang" last="Liu">Xing Liang Liu</name></author><author><name sortKey="Connors, Karin" sort="Connors, Karin" uniqKey="Connors K" first="Karin" last="Connors">Karin Connors</name></author><author><name sortKey="Matheis, Nikolaus" sort="Matheis, Nikolaus" uniqKey="Matheis N" first="Nikolaus" last="Matheis">Nikolaus Matheis</name></author><author><name sortKey="Schuster, Debra K" sort="Schuster, Debra K" uniqKey="Schuster D" first="Debra K." last="Schuster">Debra K. Schuster</name></author><author><name sortKey="Menasco, D J" sort="Menasco, D J" uniqKey="Menasco D" first="D. J." last="Menasco">D. J. Menasco</name></author><author><name sortKey="Wagoner, Wendy" sort="Wagoner, Wendy" uniqKey="Wagoner W" first="Wendy" last="Wagoner">Wendy Wagoner</name></author><author><name sortKey="Lightner, Jonathan" sort="Lightner, Jonathan" uniqKey="Lightner J" first="Jonathan" last="Lightner">Jonathan Lightner</name></author><author><name sortKey="Wagner, D Ry" sort="Wagner, D Ry" uniqKey="Wagner D" first="D. Ry" last="Wagner">D. Ry Wagner</name></author></analytic><series><title level="j">The Plant Cell</title><idno type="ISSN">1040-4651</idno><idno type="eISSN">1532-298X</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We have developed a high-throughput T-DNA insertional mutagenesis program in tomato using activation tagging to identify genes that regulate metabolic pathways. One of the activation-tagged insertion lines (<italic>ant1</italic>) showed intense purple pigmentation from the very early stage of shoot formation in culture, reflecting activation of the biosynthetic pathway leading to anthocyanin accumulation. The purple coloration resulted from the overexpression of a gene that encodes a MYB transcription factor. Vegetative tissues of <italic>ant1</italic> plants displayed intense purple color, and the fruit showed purple spotting on the epidermis and pericarp. The gene-to-trait relationship of <italic>ant1</italic> was confirmed by the overexpression of <italic>ANT1</italic> in transgenic tomato and in tobacco under the control of a constitutive promoter. Suppression subtractive hybridization and RNA hybridization analysis of the purple tomato plants indicated that the overexpression of <italic>ANT1</italic> caused the upregulation of genes that encode proteins in both the early and later steps of anthocyanidin biosynthesis as well as genes involved in the glycosylation and transport of anthocyanins into the vacuole.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell</journal-id><journal-id journal-id-type="publisher-id">plantcell</journal-id><journal-title>The Plant Cell</journal-title><issn pub-type="ppub">1040-4651</issn><issn pub-type="epub">1532-298X</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12897245</article-id><article-id pub-id-type="pmc">167162</article-id><article-id pub-id-type="publisher-id">012963</article-id><article-id pub-id-type="doi">10.1105/tpc.012963</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mathews</surname><given-names>Helena</given-names></name><xref ref-type="corresp" rid="cor1">1</xref></contrib><contrib contrib-type="author"><name><surname>Clendennen</surname><given-names>Stephanie K.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Caldwell</surname><given-names>Colby G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Xing Liang</given-names></name></contrib><contrib contrib-type="author"><name><surname>Connors</surname><given-names>Karin</given-names></name></contrib><contrib contrib-type="author"><name><surname>Matheis</surname><given-names>Nikolaus</given-names></name></contrib><contrib contrib-type="author"><name><surname>Schuster</surname><given-names>Debra K.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Menasco</surname><given-names>D. J.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wagoner</surname><given-names>Wendy</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lightner</surname><given-names>Jonathan</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wagner</surname><given-names>D. Ry</given-names></name></contrib></contrib-group><aff id="N0x99000c0.0xa125100">Exelixis Plant Sciences, Inc., Portland, Oregon 97224-7744</aff><author-notes><fn id="cor1"><label>1</label><p>To whom correspondence should be addressed. E-mail <email>hmathews@exelixis.com</email>; fax 503-670-7703</p></fn></author-notes><pub-date pub-type="ppub"><month>8</month><year>2003</year></pub-date><volume>15</volume><issue>8</issue><fpage>1689</fpage><lpage>1703</lpage><history><date date-type="received"><day>15</day><month>4</month><year>2003</year></date><date date-type="accepted"><day>9</day><month>6</month><year>2003</year></date></history><copyright-statement>Copyright © 2003, American Society of Plant Biologists</copyright-statement><copyright-year>2003</copyright-year><abstract><p>We have developed a high-throughput T-DNA insertional mutagenesis program in tomato using activation tagging to identify genes that regulate metabolic pathways. One of the activation-tagged insertion lines (<italic>ant1</italic>) showed intense purple pigmentation from the very early stage of shoot formation in culture, reflecting activation of the biosynthetic pathway leading to anthocyanin accumulation. The purple coloration resulted from the overexpression of a gene that encodes a MYB transcription factor. Vegetative tissues of <italic>ant1</italic> plants displayed intense purple color, and the fruit showed purple spotting on the epidermis and pericarp. The gene-to-trait relationship of <italic>ant1</italic> was confirmed by the overexpression of <italic>ANT1</italic> in transgenic tomato and in tobacco under the control of a constitutive promoter. Suppression subtractive hybridization and RNA hybridization analysis of the purple tomato plants indicated that the overexpression of <italic>ANT1</italic> caused the upregulation of genes that encode proteins in both the early and later steps of anthocyanidin biosynthesis as well as genes involved in the glycosylation and transport of anthocyanins into the vacuole.</p></abstract></article-meta><notes><fn-group><fn><p>Article, publication date, and citation information can be found at <ext-link ext-link-type="uri" xlink:href="http://www.plantcell.org/cgi/doi/10.1105/tpc.012963">www.plantcell.org/cgi/doi/10.1105/tpc.012963</ext-link>.</p></fn></fn-group></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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