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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri.</title><author><name sortKey="Mouches, C" sort="Mouches, C" uniqKey="Mouches C" first="C" last="Mouches">C. Mouches</name></author><author><name sortKey="Barroso, G" sort="Barroso, G" uniqKey="Barroso G" first="G" last="Barroso">G. Barroso</name></author><author><name sortKey="Bove, J M" sort="Bove, J M" uniqKey="Bove J" first="J M" last="Bové">J M Bové</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">6313631</idno><idno type="pmc">217925</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC217925</idno><idno type="RBID">PMC:217925</idno><date when="1983">1983</date><idno type="wicri:Area/Pmc/Corpus">000776</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri.</title><author><name sortKey="Mouches, C" sort="Mouches, C" uniqKey="Mouches C" first="C" last="Mouches">C. Mouches</name></author><author><name sortKey="Barroso, G" sort="Barroso, G" uniqKey="Barroso G" first="G" last="Barroso">G. Barroso</name></author><author><name sortKey="Bove, J M" sort="Bove, J M" uniqKey="Bove J" first="J M" last="Bové">J M Bové</name></author></analytic><series><title level="j">Journal of Bacteriology</title><idno type="ISSN">0021-9193</idno><idno type="eISSN">1098-5530</idno><imprint><date when="1983">1983</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obtained upon nick translation of the recombinant plasmid was used to further characterize and compare pMH1 and pM41.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jbacter00240-0485-a" xlink:role="953"></graphic></fig><fig id="F2"><graphic xlink:href="jbacter00240-0486-a" xlink:role="954"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Bacteriol</journal-id><journal-title>Journal of Bacteriology</journal-title><issn pub-type="ppub">0021-9193</issn><issn pub-type="epub">1098-5530</issn></journal-meta><article-meta><article-id pub-id-type="pmid">6313631</article-id><article-id pub-id-type="pmc">217925</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mouches</surname><given-names>C</given-names></name></contrib><contrib contrib-type="author"><name><surname>Barroso</surname><given-names>G</given-names></name></contrib><contrib contrib-type="author"><name><surname>Bové</surname><given-names>J M</given-names></name></contrib></contrib-group><pub-date pub-type="ppub"><month>11</month><year>1983</year></pub-date><volume>156</volume><issue>2</issue><fpage>952</fpage><lpage>955</lpage><abstract><p>Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obtained upon nick translation of the recombinant plasmid was used to further characterize and compare pMH1 and pM41.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jbacter00240-0485-a" xlink:role="953"></graphic></fig><fig id="F2"><graphic xlink:href="jbacter00240-0486-a" xlink:role="954"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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