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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri.</title>
<author><name sortKey="Mouches, C" sort="Mouches, C" uniqKey="Mouches C" first="C" last="Mouches">C. Mouches</name>
</author>
<author><name sortKey="Barroso, G" sort="Barroso, G" uniqKey="Barroso G" first="G" last="Barroso">G. Barroso</name>
</author>
<author><name sortKey="Bove, J M" sort="Bove, J M" uniqKey="Bove J" first="J M" last="Bové">J M Bové</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">6313631</idno>
<idno type="pmc">217925</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC217925</idno>
<idno type="RBID">PMC:217925</idno>
<date when="1983">1983</date>
<idno type="wicri:Area/Pmc/Corpus">000776</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri.</title>
<author><name sortKey="Mouches, C" sort="Mouches, C" uniqKey="Mouches C" first="C" last="Mouches">C. Mouches</name>
</author>
<author><name sortKey="Barroso, G" sort="Barroso, G" uniqKey="Barroso G" first="G" last="Barroso">G. Barroso</name>
</author>
<author><name sortKey="Bove, J M" sort="Bove, J M" uniqKey="Bove J" first="J M" last="Bové">J M Bové</name>
</author>
</analytic>
<series><title level="j">Journal of Bacteriology</title>
<idno type="ISSN">0021-9193</idno>
<idno type="eISSN">1098-5530</idno>
<imprint><date when="1983">1983</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obtained upon nick translation of the recombinant plasmid was used to further characterize and compare pMH1 and pM41.</p>
<sec sec-type="scanned-figures"><title>Images</title>
<fig id="F1"><graphic xlink:href="jbacter00240-0485-a" xlink:role="953"></graphic>
</fig>
<fig id="F2"><graphic xlink:href="jbacter00240-0486-a" xlink:role="954"></graphic>
</fig>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Bacteriol</journal-id>
<journal-title>Journal of Bacteriology</journal-title>
<issn pub-type="ppub">0021-9193</issn>
<issn pub-type="epub">1098-5530</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">6313631</article-id>
<article-id pub-id-type="pmc">217925</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri.</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Mouches</surname>
<given-names>C</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Barroso</surname>
<given-names>G</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Bové</surname>
<given-names>J M</given-names>
</name>
</contrib>
</contrib-group>
<pub-date pub-type="ppub"><month>11</month>
<year>1983</year>
</pub-date>
<volume>156</volume>
<issue>2</issue>
<fpage>952</fpage>
<lpage>955</lpage>
<abstract><p>Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obtained upon nick translation of the recombinant plasmid was used to further characterize and compare pMH1 and pM41.</p>
<sec sec-type="scanned-figures"><title>Images</title>
<fig id="F1"><graphic xlink:href="jbacter00240-0485-a" xlink:role="953"></graphic>
</fig>
<fig id="F2"><graphic xlink:href="jbacter00240-0486-a" xlink:role="954"></graphic>
</fig>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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