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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Diversity of Endophytic Bacterial Populations and Their Interaction with <italic>Xylella fastidiosa</italic> in Citrus Plants</title><author><name sortKey="Araujo, Welington L" sort="Araujo, Welington L" uniqKey="Araujo W" first="Welington L." last="Araújo">Welington L. Araújo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Marcon, Joelma" sort="Marcon, Joelma" uniqKey="Marcon J" first="Joelma" last="Marcon">Joelma Marcon</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Maccheroni, Walter" sort="Maccheroni, Walter" uniqKey="Maccheroni W" first="Walter" last="Maccheroni">Walter Maccheroni</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Van Elsas, Jan Dirk" sort="Van Elsas, Jan Dirk" uniqKey="Van Elsas J" first="Jan Dirk" last="Van Elsas">Jan Dirk Van Elsas</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Van Vuurde, Jim W L" sort="Van Vuurde, Jim W L" uniqKey="Van Vuurde J" first="Jim W. L." last="Van Vuurde">Jim W. L. Van Vuurde</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12324338</idno><idno type="pmc">126398</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC126398</idno><idno type="RBID">PMC:126398</idno><idno type="doi">10.1128/AEM.68.10.4906-4914.2002</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">000742</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Diversity of Endophytic Bacterial Populations and Their Interaction with <italic>Xylella fastidiosa</italic> in Citrus Plants</title><author><name sortKey="Araujo, Welington L" sort="Araujo, Welington L" uniqKey="Araujo W" first="Welington L." last="Araújo">Welington L. Araújo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Marcon, Joelma" sort="Marcon, Joelma" uniqKey="Marcon J" first="Joelma" last="Marcon">Joelma Marcon</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Maccheroni, Walter" sort="Maccheroni, Walter" uniqKey="Maccheroni W" first="Walter" last="Maccheroni">Walter Maccheroni</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Van Elsas, Jan Dirk" sort="Van Elsas, Jan Dirk" uniqKey="Van Elsas J" first="Jan Dirk" last="Van Elsas">Jan Dirk Van Elsas</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Van Vuurde, Jim W L" sort="Van Vuurde, Jim W L" uniqKey="Van Vuurde J" first="Jim W. L." last="Van Vuurde">Jim W. L. Van Vuurde</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Applied and Environmental Microbiology</title><idno type="ISSN">0099-2240</idno><idno type="eISSN">1098-5336</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Citrus variegated chlorosis (CVC) is caused by <italic>Xylella fastidiosa</italic>, a phytopathogenic bacterium that can infect all <italic>Citrus sinensis</italic> cultivars. The endophytic bacterial communities of healthy, resistant, and CVC-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. The endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (DGGE). Dominant isolates were characterized by fatty-acid methyl ester analysis as <italic>Bacillus pumilus</italic>, <italic>Curtobacterium flaccumfaciens</italic>, <italic>Enterobacter cloacae</italic>, <italic>Methylobacterium</italic> spp. (including <italic>Methylobacterium extorquens</italic>, <italic>M. fujisawaense</italic>, <italic>M. mesophilicum</italic>, <italic>M. radiotolerans</italic>, and <italic>M. zatmanii</italic>), <italic>Nocardia</italic> sp., <italic>Pantoea agglomerans</italic>, and <italic>Xanthomonas campestris</italic>. We observed a relationship between CVC symptoms and the frequency of isolation of species of <italic>Methylobacterium</italic>, the genus that we most frequently isolated from symptomatic plants. In contrast, we isolated <italic>C. flaccumfaciens</italic> significantly more frequently from asymptomatic plants than from those with symptoms of CVC while <italic>P. agglomerans</italic> was frequently isolated from tangerine (<italic>Citrus reticulata</italic>) and sweet-orange (<italic>C. sinensis</italic>) plants, irrespective of whether the plants were symptomatic or asymptomatic or showed symptoms of CVC. DGGE analysis of 16S rRNA gene fragments amplified from total plant DNA resulted in several bands that matched those from the bacterial isolates, indicating that DGGE profiles can be used to detect some endophytic bacteria of citrus plants. However, some bands had no match with any isolate, suggesting the occurrence of other, nonculturable or as yet uncultured, endophytic bacteria. A specific band with a high G+C ratio was observed only in asymptomatic plants. The higher frequency of <italic>C. flaccumfaciens</italic> in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id><journal-id journal-id-type="publisher-id">aem</journal-id><journal-title>Applied and Environmental Microbiology</journal-title><issn pub-type="ppub">0099-2240</issn><issn pub-type="epub">1098-5336</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12324338</article-id><article-id pub-id-type="pmc">126398</article-id><article-id pub-id-type="publisher-id">0387</article-id><article-id pub-id-type="doi">10.1128/AEM.68.10.4906-4914.2002</article-id><article-categories><subj-group subj-group-type="heading"><subject>Plant Microbiology</subject></subj-group></article-categories><title-group><article-title>Diversity of Endophytic Bacterial Populations and Their Interaction with <italic>Xylella fastidiosa</italic> in Citrus Plants</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Araújo</surname><given-names>Welington L.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Marcon</surname><given-names>Joelma</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Maccheroni</surname><given-names>Walter</given-names><suffix>Jr.</suffix></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>van Elsas</surname><given-names>Jan Dirk</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>van Vuurde</surname><given-names>Jim W. L.</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Azevedo</surname><given-names>João Lúcio</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff1">3</xref></contrib></contrib-group><aff id="aff1">Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz,” Universidade de São Paulo, Piracicaba,<label>1</label> Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, São Paulo, Brazil,<label>3</label> Plant Research International, Wageningen University and Research Center, Wageningen, The Netherlands<label>2</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ/USP), Avenida Pádua Dias, 11, P. O. Box 83, 13400-970 Piracicaba, SP, Brazil. Phone: 55 19 3429 4251. Fax: 55 19 3433 6706. E-mail: <email>wlaraujo@esalq.usp.br</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>10</month><year>2002</year></pub-date><volume>68</volume><issue>10</issue><fpage>4906</fpage><lpage>4914</lpage><history><date date-type="received"><day>4</day><month>3</month><year>2002</year></date><date date-type="accepted"><day>10</day><month>7</month><year>2002</year></date></history><copyright-statement>Copyright © 2002, American Society for Microbiology</copyright-statement><copyright-year>2002</copyright-year><abstract><p>Citrus variegated chlorosis (CVC) is caused by <italic>Xylella fastidiosa</italic>, a phytopathogenic bacterium that can infect all <italic>Citrus sinensis</italic> cultivars. The endophytic bacterial communities of healthy, resistant, and CVC-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. The endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (DGGE). Dominant isolates were characterized by fatty-acid methyl ester analysis as <italic>Bacillus pumilus</italic>, <italic>Curtobacterium flaccumfaciens</italic>, <italic>Enterobacter cloacae</italic>, <italic>Methylobacterium</italic> spp. (including <italic>Methylobacterium extorquens</italic>, <italic>M. fujisawaense</italic>, <italic>M. mesophilicum</italic>, <italic>M. radiotolerans</italic>, and <italic>M. zatmanii</italic>), <italic>Nocardia</italic> sp., <italic>Pantoea agglomerans</italic>, and <italic>Xanthomonas campestris</italic>. We observed a relationship between CVC symptoms and the frequency of isolation of species of <italic>Methylobacterium</italic>, the genus that we most frequently isolated from symptomatic plants. In contrast, we isolated <italic>C. flaccumfaciens</italic> significantly more frequently from asymptomatic plants than from those with symptoms of CVC while <italic>P. agglomerans</italic> was frequently isolated from tangerine (<italic>Citrus reticulata</italic>) and sweet-orange (<italic>C. sinensis</italic>) plants, irrespective of whether the plants were symptomatic or asymptomatic or showed symptoms of CVC. DGGE analysis of 16S rRNA gene fragments amplified from total plant DNA resulted in several bands that matched those from the bacterial isolates, indicating that DGGE profiles can be used to detect some endophytic bacteria of citrus plants. However, some bands had no match with any isolate, suggesting the occurrence of other, nonculturable or as yet uncultured, endophytic bacteria. A specific band with a high G+C ratio was observed only in asymptomatic plants. The higher frequency of <italic>C. flaccumfaciens</italic> in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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