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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Oolong tea made from tea plants from different locations in Yunnan and Fujian, China showed similar aroma but different taste characteristics</title><author><name sortKey="Wang, Chen" sort="Wang, Chen" uniqKey="Wang C" first="Chen" last="Wang">Chen Wang</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Lv, Shidong" sort="Lv, Shidong" uniqKey="Lv S" first="Shidong" last="Lv">Shidong Lv</name><affiliation><nlm:aff id="Aff2">Kunming Grain & Oil and Feed Product Quality Inspection Center, Kunming, 650118 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Wu, Yuanshuang" sort="Wu, Yuanshuang" uniqKey="Wu Y" first="Yuanshuang" last="Wu">Yuanshuang Wu</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Gao, Xuemei" sort="Gao, Xuemei" uniqKey="Gao X" first="Xuemei" last="Gao">Xuemei Gao</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Li, Jiangbing" sort="Li, Jiangbing" uniqKey="Li J" first="Jiangbing" last="Li">Jiangbing Li</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Zhang, Wenrui" sort="Zhang, Wenrui" uniqKey="Zhang W" first="Wenrui" last="Zhang">Wenrui Zhang</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Meng, Qingxiong" sort="Meng, Qingxiong" uniqKey="Meng Q" first="Qingxiong" last="Meng">Qingxiong Meng</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27247873</idno><idno type="pmc">4864747</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864747</idno><idno type="RBID">PMC:4864747</idno><idno type="doi">10.1186/s40064-016-2229-y</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000702</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Oolong tea made from tea plants from different locations in Yunnan and Fujian, China showed similar aroma but different taste characteristics</title><author><name sortKey="Wang, Chen" sort="Wang, Chen" uniqKey="Wang C" first="Chen" last="Wang">Chen Wang</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Lv, Shidong" sort="Lv, Shidong" uniqKey="Lv S" first="Shidong" last="Lv">Shidong Lv</name><affiliation><nlm:aff id="Aff2">Kunming Grain & Oil and Feed Product Quality Inspection Center, Kunming, 650118 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Wu, Yuanshuang" sort="Wu, Yuanshuang" uniqKey="Wu Y" first="Yuanshuang" last="Wu">Yuanshuang Wu</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Gao, Xuemei" sort="Gao, Xuemei" uniqKey="Gao X" first="Xuemei" last="Gao">Xuemei Gao</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Li, Jiangbing" sort="Li, Jiangbing" uniqKey="Li J" first="Jiangbing" last="Li">Jiangbing Li</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Zhang, Wenrui" sort="Zhang, Wenrui" uniqKey="Zhang W" first="Wenrui" last="Zhang">Wenrui Zhang</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author><author><name sortKey="Meng, Qingxiong" sort="Meng, Qingxiong" uniqKey="Meng Q" first="Qingxiong" last="Meng">Qingxiong Meng</name><affiliation><nlm:aff id="Aff1">Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</nlm:aff></affiliation></author></analytic><series><title level="j">SpringerPlus</title><idno type="eISSN">2193-1801</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Consistent aroma characteristics are important for tea products. However, understanding the formation of tea aroma flavor and correspondingly proposing applicable protocols to control tea quality and consistency remain major challenges. Oolong tea is one of the most popular teas with a distinct flavor. Generally, oolong tea is processed with the leaves of tea trees belonging to different subspecies and grown in significantly different regions. In this study, Yunnan and Fujian oolong teas, green tea, black tea, and Pu-erh tea were collected from major tea estates across China. Their sensory evaluation, main water-soluble and volatile compounds were identified and measured. The sensory evaluation, total polysaccharide, caffeine, and catechin content of Yunnan oolong tea was found to be different from that of Fujian oolong tea, a result suggesting that the kinds of tea leaves used in Yunnan and Fujian oolong teas were naturally different. However, according to their aroma compounds, principal component analysis (PCA) and cluster analysis (CA) of the volatile compounds showed that the two types of oolong teas were similar and cannot be clearly distinguished from each other; they are also different from green, black, and Pu-erh teas, a result indicating that the same oolong tea processing technology applied to different tea leaves results in consistent aroma characteristics. The PCA analysis results also indicated that benzylalcohol, indole, safranal, linalool oxides, <italic>β</italic>-ionone, and hexadecanoic acid methyl ester highly contributed to the distinct aroma of oolong tea compared with the other three types of teas. This study proved that the use of the same processing technology on two kinds of tea leaves resulted in a highly consistent tea aroma.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Baldermann, S" uniqKey="Baldermann S">S Baldermann</name></author><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z Yang</name></author><author><name sortKey="Katsuno, T" uniqKey="Katsuno T">T Katsuno</name></author><author><name sortKey="Tu, Va" uniqKey="Tu V">VA Tu</name></author><author><name sortKey="Mase, N" uniqKey="Mase N">N Mase</name></author><author><name sortKey="Nakamura, Y" uniqKey="Nakamura Y">Y Nakamura</name></author><author><name sortKey="Watanabe, N" uniqKey="Watanabe N">N Watanabe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bode, Am" uniqKey="Bode A">AM Bode</name></author><author><name sortKey="Dong, Z" uniqKey="Dong Z">Z 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Springerplus</journal-id><journal-id journal-id-type="iso-abbrev">Springerplus</journal-id><journal-title-group><journal-title>SpringerPlus</journal-title></journal-title-group><issn pub-type="epub">2193-1801</issn><publisher><publisher-name>Springer International Publishing</publisher-name><publisher-loc>Cham</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27247873</article-id><article-id pub-id-type="pmc">4864747</article-id><article-id pub-id-type="publisher-id">2229</article-id><article-id pub-id-type="doi">10.1186/s40064-016-2229-y</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group></article-categories><title-group><article-title>Oolong tea made from tea plants from different locations in Yunnan and Fujian, China showed similar aroma but different taste characteristics</article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Wang</surname><given-names>Chen</given-names></name><address><email>chenwang1224@hotmail.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Lv</surname><given-names>Shidong</given-names></name><address><email>shidonglv@163.com</email></address><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Yuanshuang</given-names></name><address><email>wyswu@hotmail.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Gao</surname><given-names>Xuemei</given-names></name><address><email>beyond4amni@163.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Jiangbing</given-names></name><address><email>ljb216970@sina.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Wenrui</given-names></name><address><email>felix-chang@live.cn</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Meng</surname><given-names>Qingxiong</given-names></name><address><phone>+86 871 65920541</phone><email>qxmeng@scbg.ac.cn</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><label></label>Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500 Yunnan People’s Republic of China</aff><aff id="Aff2"><label></label>Kunming Grain & Oil and Feed Product Quality Inspection Center, Kunming, 650118 Yunnan People’s Republic of China</aff></contrib-group><pub-date pub-type="epub"><day>10</day><month>5</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>10</day><month>5</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>5</volume><elocation-id>576</elocation-id><history><date date-type="received"><day>22</day><month>12</month><year>2015</year></date><date date-type="accepted"><day>25</day><month>4</month><year>2016</year></date></history><permissions><copyright-statement>© The Author(s). 2016</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.</license-p></license></permissions><abstract id="Abs1"><p>Consistent aroma characteristics are important for tea products. However, understanding the formation of tea aroma flavor and correspondingly proposing applicable protocols to control tea quality and consistency remain major challenges. Oolong tea is one of the most popular teas with a distinct flavor. Generally, oolong tea is processed with the leaves of tea trees belonging to different subspecies and grown in significantly different regions. In this study, Yunnan and Fujian oolong teas, green tea, black tea, and Pu-erh tea were collected from major tea estates across China. Their sensory evaluation, main water-soluble and volatile compounds were identified and measured. The sensory evaluation, total polysaccharide, caffeine, and catechin content of Yunnan oolong tea was found to be different from that of Fujian oolong tea, a result suggesting that the kinds of tea leaves used in Yunnan and Fujian oolong teas were naturally different. However, according to their aroma compounds, principal component analysis (PCA) and cluster analysis (CA) of the volatile compounds showed that the two types of oolong teas were similar and cannot be clearly distinguished from each other; they are also different from green, black, and Pu-erh teas, a result indicating that the same oolong tea processing technology applied to different tea leaves results in consistent aroma characteristics. The PCA analysis results also indicated that benzylalcohol, indole, safranal, linalool oxides, <italic>β</italic>-ionone, and hexadecanoic acid methyl ester highly contributed to the distinct aroma of oolong tea compared with the other three types of teas. This study proved that the use of the same processing technology on two kinds of tea leaves resulted in a highly consistent tea aroma.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Oolong tea</kwd><kwd>Main water-soluble contents</kwd><kwd>Volatile compounds</kwd><kwd>Processing technology</kwd><kwd>Aroma characteristics</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100001809</institution-id><institution>National Natural Science Foundation of China</institution></institution-wrap></funding-source><award-id>31460228</award-id><principal-award-recipient><name><surname>Meng</surname><given-names>Qingxiong</given-names></name></principal-award-recipient></award-group><award-group><funding-source><institution>Scientific research funds in Yunnan province department of education</institution></funding-source><award-id>2014Y089</award-id><principal-award-recipient><name><surname>Wu</surname><given-names>Yuanshuang</given-names></name></principal-award-recipient></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2016</meta-value></custom-meta></custom-meta-group></article-meta></front><body><sec id="Sec1"><title>Background</title><p>Oolong tea is a kind of partially fermented tea. It has become one of the most popular beverages in China because of its sweet grassy taste and unique flower-like aroma. Traditional oolong tea is produced with <italic>Camellia</italic><italic>sinensis</italic> var. <italic>sinensis</italic> (China type) from Fujian Province in southeast China. However, because of the limited raw tea produced in Fujian Province, tea buds grown in other locations and from different tea tree subspecies have also been used for oolong tea in recent years; an example is <italic>Camellia sinensis</italic> var. <italic>assamica</italic> (Assam type), which is mainly distributed in Yunnan Province in southwest China, especially the districts around Pu-erh (Lv et al. <xref ref-type="bibr" rid="CR12">2014a</xref>). These two tea subspecies grown in different locations show obvious differences, such as the sizes of their leaves and their water-soluble components (Liang et al. <xref ref-type="bibr" rid="CR11">2005</xref>). Until now, however, little known about the similarities and dissimilarities of these two oolong teas. To determine their consistency in taste, we analyzed the caffeine, catechin, total polysaccharide, and volatile components of Yunnan and Fujian oolong teas and compared them with those of other common types of teas.</p><p>Main water-soluble components, such as caffeine, polysaccharides, and catechins (Zhu et al. <xref ref-type="bibr" rid="CR30">2015</xref>; Nie et al. <xref ref-type="bibr" rid="CR17">2011</xref>), are generally responsible for the taste of tea fusion, whereas volatile components contribute to tea aroma. In tea, volatile components are only present in about 0.01 % of the total dry weight, but they result in a high odor experience because of their low threshold value (Rawat et al. <xref ref-type="bibr" rid="CR19">2007</xref>). Whereas water-soluble content is naturally influenced by geographical characteristics, climate, tea cultivar, and processing technology applied on raw leaves, volatile compound content can be influenced and transformed by the processing technology used on the leaves (Fernández-Cáceres et al. <xref ref-type="bibr" rid="CR6">2001</xref>; Narukawa et al. <xref ref-type="bibr" rid="CR16">2011</xref>). Volatile compounds are transformed from water-soluble components during processing steps, such as fermentation, post-fermentation, and baking (Hara et al. <xref ref-type="bibr" rid="CR8">1995</xref>). For example, Yunnan and Fujian oolong teas are both partially fermented by the same processing technology with a series of steps, and they show sweet, fruity, and flower-like odors. Green tea, which is not fermented, has a fresh, grassy flavor. Black tea, which is fully fermented, has a honey, flower-like flavor. Pu-erh tea, which is post-microbially fermented, has a woody and stale flavor (Lv et al. <xref ref-type="bibr" rid="CR14">2015</xref>). Whether teas produced from the same types of tea leaves coming from different tea trees show similar or different aroma characteristics has not been extensively studied. For finding the similarity and differences of volatile and water-soluble compounds between Yunnan and Fujian oolong tea, we compared them with those of other kinds of tea to decrease the noises from the data of oolong teas.</p><p>In this study, the sensory evaluation, main water-soluble (i.e., caffeine, catechins, and total polysaccharides) and volatile components of Yunnan oolong, Fujian oolong, green, black, and Pu-erh teas were analyzed, and the aroma consistency of oolong teas made from different tea tree leaves was discussed.</p></sec><sec id="Sec2"><title>Methods</title><sec id="Sec3"><title>Tea samples</title><p>Five samples of Yunnan oolong tea were obtained from five typical production sites in Yunnan Province, China and were numbered from YO1 to YO5. Five samples of Fujian oolong tea were also obtained from five typical production sites in Fujian Province, China and were numbered from FO1 to FO5. Ten samples of green tea were likewise collected from Hunan, Yunnan, Sichuan, and Anhui provinces and were numbered from GT1 to GT10, and ten samples of black tea were collected from Yunnan, Anhui, Fujian, and Hunan provinces and were numbered from BT1 to BT10. Finally, 10 samples of Pu-erh tea were collected from Yunnan Province, China and were numbered from CT1 to CT10. In addition, all the tea samples were harvested in spring, 2015; and the varieties of them identified by National Centre for Pu-erh Tea Production Quality Supervision and Inspection, Pu-erh, Yunnan, China.</p></sec><sec id="Sec4"><title>Chemicals</title><p>The following chemicals and solvents were used: (+)-Catechin (C, ≥ 99 %), (−)-epicatechin (EC, ≥ 98 %), (−)-epigallocatechin (EGC, ≥ 95 %), (−)-epigallocatechin gallate (EGCG, ≥ 95 %), and (−)-epicatechin gallate (ECG, ≥ 98 %) were obtained from Sigma-Aldrich (St. Louis, MO, USA.). Methanol (HPLC grade, ≥ 99.9 %, Lichrosolv, Germany) and acetic acid (HPLC grade, ≥ 99.7 %) were obtained from Fisher Scientific. All other reagents and solvents were of analytical grade and used without further purification, unless otherwise noted. All aqueous solutions were prepared with the use of newly double-distilled water.</p></sec><sec id="Sec5"><title>Sensory evaluation</title><p>According to the CNIS GB/T 14487-93, three grams of tea sample was extracted with 300 mL of 85 °C distilled water for 15 min. The extracted tea infusion was filtered and cooled to room temperature and then adjusted to a volume of 500 mL. Then the sensory characteristics of the extracted tea infusions were evaluated by five panelists at Faculty of Life Science and Technology, Kunming University of Science and Technology, based on the color, taste and flavor of tea infusions.</p></sec><sec id="Sec6"><title>Catechin and caffeine analysis</title><p>Samples weighing 0.2 ± 0.001 g were placed in extraction tubes (10 mL). Five milliliters of preheated 70 % water/methanol extraction mixture was filled into each tube individually, incubated in water bath for 10 min at 70 °C, and vortexed for 5 and 10 min, respectively. The extracts were combined and made up to 10 mL with cold methanol/water extraction mixture.</p><p>The content and composition of catechins and caffeine in the extract were determined with an HPLC system (2695; Waters Corp., MA, USA) equipped with a Waters Sunfire C<sub>18</sub> column (5, 4.6 × 250 mm, 35 °C) at 278 nm. The measurement was adjusted as follows: flow rate: 1.0 mL/min; injection volume: 10 μL; mobile phase: A 98 % methanol and 2 % acetic acid, B 98 % water and 2 % acetic acid; gradient elution: 20–25 % A, 0–1 min; 25–45 % A, 1–12 min; 45–90 % A, 12–14.3 min; 90–20 % A, 14.3–15 min; maintained for 5 min. Concentrations of catechins and caffeine were quantified by their peak areas against those of standards prepared from authentic compounds.</p></sec><sec id="Sec7"><title>Determination of total polysaccharides</title><p>Total polysaccharides were measured according to the method described by Xi et al. (<xref ref-type="bibr" rid="CR28">2010</xref>). The dry, ground tea leaves (50 g) were extracted with 400 mL distilled water at 90 °C in a water bath for 2 h. After being filtered, the residue was extracted again with 500 mL distilled water for another 2 h. Then, the extracts were centrifuged to remove contaminants. The supernatant was concentrated via rotary evaporation and precipitated with 95 % ethanol. The tea extracts were measured with this method.</p></sec><sec id="Sec8"><title>HS-SPME procedure</title><p>The HS-SPME parameters of the tea sample were validated and optimized in a previous study (Lv et al. <xref ref-type="bibr" rid="CR13">2014b</xref>). Therefore, the same method and parameters were used in the current study to extract the volatile components of the tea samples. Using the same method is advantageous in tracing the change in aroma compounds during the production of the tea sample and in facilitating a comprehensive comparison of the aroma components among four different tea samples. A detailed explanation of the HS-SPME parameters is as follows.</p><p>A total of 2.0 g ground tea sample was placed in a 20 mL sealed headspace vial with 5 mL distilled water, and the temperature of the headspace vial was kept at 80 °C for 60 min with an electric hot plate. Then, a 65 μm polydimethylsiloxane/divinylbenzene coating fiber (Supelco Inc., Bellefonte, PA) was exposed to the sample headspace and retained for 60 min. All volatile compounds absorbed on the SPME fiber were desorbed at the GC–MS injector at 250 °C for 3.5 min and then immediately analyzed by GC–MS. After adsorption, SPME coating fiber was transferred to the GC injection port at 250 °C for 30 min.</p></sec><sec id="Sec9"><title>GC–MS analysis</title><p>An HP 7890A GC instrument combined with an HP 5975C mass selective detector (MSD) quadrupole MS instrument (Agilent Technologies, Palo Alto, CA, USA) was used for the GC–MS analysis. The capillary column utilized was HP-5MS (30 m × 0.25 mm × 0.25 μm film thickness) from Agilent technologies, and high-purity helium (purity 99.999 %) was used as carrier gas at a flow rate of 1 mL/min. The injector and ion source temperatures were set at 250 and 200 °C, respectively. Samples were injected in splitless mode. The initial GC oven temperature was 50 °C, held for 5 min, and then ramped at 3 °C/min to 210 °C, held for 3 min, and finally programmed to 230 °C at 15 °C/min. The Agilent 5975C MS was operated in the electron impact mode using ionization energy of 70 eV with an ionization source temperature of 230 °C and a quadrupole set of 150 °C. The acquisition mode was full scan (from 30 to 500 m/z), and the solvent delay time was 2.8 min.</p></sec><sec id="Sec10"><title>Compound identification</title><p>With the use of the MSD G1701EA E.02.00.493 chemical workstation data processing system (Agilent Technologies, Palo Alto, CA, USA), peak identifications were made via a search of the National Institute of Standards and Technology (NIST) 08.L MS data library (Qiao et al. <xref ref-type="bibr" rid="CR18">2008</xref>; Schuh and Schieberle <xref ref-type="bibr" rid="CR20">2006</xref>). The relative percentage content of the aroma components was determined by peak area normalization.</p><p>The relative proportions of the constituents were obtained by peak area normalization. Quantitative results were obtained by using the method as follows:<disp-formula id="Equa"><alternatives><tex-math id="M1">\documentclass[12pt]{minimal}
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\begin{document}$${\text{Relative content}} \,\left( \% \right) = {{\text{single constituent area}} \mathord{\left/ {\vphantom {{\text{single constituent area}} {\text{total area}}}} \right. \kern-0pt} {\text{total area}}} \times 100\,\%$$\end{document}</tex-math><mml:math id="M2" display="block"><mml:mrow><mml:mrow><mml:mtext>Relative content</mml:mtext></mml:mrow><mml:mspace width="0.166667em"></mml:mspace><mml:mfenced close=")" open="("><mml:mo>%</mml:mo></mml:mfenced><mml:mo>=</mml:mo><mml:mrow><mml:mrow><mml:mtext>single constituent area</mml:mtext></mml:mrow><mml:mrow><mml:mfenced close="" open="/" separators=""><mml:mrow></mml:mrow></mml:mfenced><mml:mspace width="0.0pt"></mml:mspace></mml:mrow><mml:mrow><mml:mtext>total area</mml:mtext></mml:mrow></mml:mrow><mml:mo>×</mml:mo><mml:mn>100</mml:mn><mml:mspace width="0.166667em"></mml:mspace><mml:mo>%</mml:mo></mml:mrow></mml:math><graphic xlink:href="40064_2016_2229_Article_Equa.gif" position="anchor"></graphic></alternatives></disp-formula></p></sec><sec id="Sec11"><title>Data analysis</title><p>Significant differences between four different types of tea samples for each of the aroma compounds were determined by Duncan’s multiple range test analysis using SPSS statistical package (version 17.0 for Windows, SPSS, Inc., Chicago, IL, USA). PCA and CA were performed with SIMCA-P software (version 12.0, Umetrics, Umea, Sweden).</p></sec></sec><sec id="Sec12"><title>Results and discussion</title><sec id="Sec13"><title>Sensory evaluation</title><p>Sensory evaluation of extracted tea infusions was performed in this work. As shown in Fig. <xref rid="Fig1" ref-type="fig">1</xref>, the following scales were used to rank the intensity of these nine attributes: very strong-5.0, strong-4.0, fairly strong-3.0, weak-2.0, very weak-1.0. The results showed that the sensory quality of Yunnan and Fujian oolong teas both were flower-like flavor and sweet, fruity taste; but Yunnan oolong tea infusion showed more bitterness and less sweet than Fujian oolong tea; green tea infusions showed grassy flavor and fresh taste; Black tea has a fruity, flower-like flavor and sweet, honey taste; and Pu-erh tea has a woody, stale flavor and the taste of slight bitterness and astringency. In addition, Fig. <xref rid="Fig2" ref-type="fig">2</xref> showed the differences among the color of Yunnan oolong tea, Fujian oolong tea and other kinds of tea infusions. The color of Pu-erh tea infusion was darkest while that of Yunnan oolong tea infusion was lightest.<fig id="Fig1"><label>Fig. 1</label><caption><p>Spider diagram of the sensory evaluation</p></caption><graphic xlink:href="40064_2016_2229_Fig1_HTML" id="MO2"></graphic></fig><fig id="Fig2"><label>Fig. 2</label><caption><p>The<italic> shapes</italic> and tea soup color of different types of tea</p></caption><graphic xlink:href="40064_2016_2229_Fig2_HTML" id="MO3"></graphic></fig></p></sec><sec id="Sec14"><title>Analysis of the main water-soluble components of Fujian oolong tea, Yunnan oolong tea, green tea, black tea, and Pu-erh tea</title><p>Polysaccharides, caffeine, and catechins, which are highly soluble in water, in tea leaf shoots play a significant role in tea quality (Willson and Clifford <xref ref-type="bibr" rid="CR25">1992</xref>). Table <xref rid="Tab1" ref-type="table">1</xref> shows that the caffeine, catechin, and total polysaccharide content of Yunnan and Fujian oolong teas was different (P < 0.05); green, black, and Pu-erh teas had a higher caffeine content than oolong tea (P < 0.05); green tea had the highest catechin content among the five types of teas (P < 0.05), whereas black tea had the highest polysaccharide content. After being semi-fermented, most of the oolong teas, including the Yunnan and Fujian oolong teas, had little catechin content, and their polysaccharide content decreased as well. Yunnan oolong tea had the lowest polysaccharide content among the five types of teas (P < 0.05). Our results were consistent with Wang et al. (<xref ref-type="bibr" rid="CR21">2000</xref>); Xi et al. (<xref ref-type="bibr" rid="CR28">2010</xref>); Wang et al. (<xref ref-type="bibr" rid="CR24">2011</xref>). The findings indicated that <italic>Camellia</italic><italic>sinensis</italic> var. <italic>sinensis</italic> and var. <italic>assamica</italic> of the oolong tea samples, i.e., Fujian and Yunnan oolong teas, respectively, were naturally different.<table-wrap id="Tab1"><label>Table 1</label><caption><p>Total polysaccharides and catechins contents (mg g<sup>−1</sup>) in Yunnan oolong teas, Fujian oolong tea, Green teas, Black teas, and Pu-erh teas</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left">Compound</th><th align="left">Yunnan oolong tea (n = 5)</th><th align="left">Fujian oolong tea (n = 5)</th><th align="left">Green tea (n = 10)</th><th align="left">Black tea (n = 10)</th><th align="left">Pu-erh tea (n = 10)</th></tr></thead><tbody><tr><td align="left">EGC</td><td char="±" align="char">10.31 ± 2.54a<sup>*</sup></td><td char="±" align="char">14.15 ± 3.80b</td><td char="±" align="char">13.90 ± 5.51b</td><td char="±" align="char">0.37 ± 0.20c</td><td char="±" align="char">1.19 ± 0.24c</td></tr><tr><td align="left">C</td><td char="±" align="char">2.46 ± 0.94a</td><td char="±" align="char">4.84 ± 1.12b</td><td char="±" align="char">6.29 ± 2.36b</td><td char="±" align="char">1.04 ± 0.69a</td><td char="±" align="char">1.96 ± 0.41a</td></tr><tr><td align="left">EC</td><td char="±" align="char">2.15 ± 0.85a</td><td char="±" align="char">4.29 ± 0.65b</td><td char="±" align="char">5.82 ± 2.22c</td><td char="±" align="char">1.38 ± 0.73a</td><td char="±" align="char">1.36 ± 0.48a</td></tr><tr><td align="left">EGCG</td><td char="±" align="char">31.87 ± 8.35a</td><td char="±" align="char">38.24 ± 8.75b</td><td char="±" align="char">50.56 ± 8.04c</td><td char="±" align="char">3.43 ± 1.02d</td><td char="±" align="char">0.13 ± 0.08d</td></tr><tr><td align="left">ECG</td><td char="±" align="char">4.07 ± 0.69a</td><td char="±" align="char">8.24 ± 2.68b</td><td char="±" align="char">17.61 ± 3.39c</td><td char="±" align="char">3.53 ± 1.40a</td><td char="±" align="char">0.18 ± 0.16d</td></tr><tr><td align="left">Total polysaccharides</td><td char="±" align="char">14.00 ± 2.41a</td><td char="±" align="char">18.52 ± 1.53c</td><td char="±" align="char">10.31 ± 1.50b</td><td char="±" align="char">18.33 ± 2.47c</td><td char="±" align="char">17.54 ± 1.73c</td></tr><tr><td align="left">Caffeine</td><td char="±" align="char">14.56 ± 3.27a</td><td char="±" align="char">16.20 ± 5.48b</td><td char="±" align="char">26.53 ± 7.65c</td><td char="±" align="char">21.17 ± 2.72d</td><td char="±" align="char">22.61 ± 5.8d</td></tr></tbody></table><table-wrap-foot><p><italic>EGC</italic> (−)-epigallocatechin, <italic>C</italic> (+)-catechin, <italic>EC</italic> (−)-epicatechin, <italic>EGCG</italic> (−)-epigallocatechin gallate, <italic>ECG</italic> (−)-epicatechin gallate</p><p><sup>*</sup>For each parameter, different letters within a row indicate difference between different types of tea with Duncan’s multiple range test (P < 0.05)</p></table-wrap-foot></table-wrap></p></sec><sec id="Sec15"><title>Analysis of the volatile compounds of Fujian oolong tea, Yunnan oolong tea, green tea, black tea, and Pu-erh tea</title><p>Table <xref rid="Tab2" ref-type="table">2</xref> shows that a total of 92 aroma compounds were identified in all 40 tea samples. No significant difference between the most volatile compounds of Yunnan oolong tea and Fujian oolong tea was observed (P > 0.05). To differentiate oolong tea from other types of teas, 1-hexanol content served as a valuable index (P < 0.05). Compared with those in other types of teas, benzylalcohol, indole, safranal, linalool oxides, <italic>β</italic>-ionone, and hexadecanoic acid methyl ester were the volatile compounds detected in most of the oolong tea samples (Table <xref rid="Tab2" ref-type="table">2</xref>). These compounds are possibly principal contributors to the fragrant flowery aroma of oolong tea. Their abundant concentrations in oolong tea might be formed during tea manufacture, in which the hydrolysis of their glycosidase and primeverosides by <italic>β</italic>-glucosidase is intensive (Wang et al. <xref ref-type="bibr" rid="CR22">2001</xref>). However, some differences were still observed in the volatile compound content of Yunnan and Fujian oolong teas. The 1-Pentanol and 1-octen-3-ol content of Fujian oolong tea was higher than that of Yunnan oolong tea (P < 0.05), whereas the benzaldehyde content of Yunnan oolong tea was higher than that of Fujian oolong tea. These subtle differences should be related to the natural differences of the tea leaves used, as observed in the water-soluble components. Because the most volatile compounds are transformed during fermentation or processing, hypothesizing that these minor differences can mostly be eliminated by the adjustment of processing conditions is reasonable. Generally, the fermentation degree of oolong tea is between that of green and black tea. Therefore, more complicated patterns of aroma flavors can be observed in semi-fermented oolong tea than in unfermented green or fully fermented black tea.<table-wrap id="Tab2"><label>Table 2</label><caption><p>Volatile components and their relative contents in Yunnan oolong teas, Fujian oolong tea, Green teas, Black teas, and Pu-erh teas</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left">No.</th><th align="left">Retention time</th><th align="left">Compound</th><th align="left">Yunnan oolong tea (n = 5)</th><th align="left">Fujian oolong tea (n = 5)</th><th align="left">Green tea (n = 10)</th><th align="left">Black tea (n = 10)</th><th align="left">Pu-erh tea (n = 10)</th></tr></thead><tbody><tr><td align="left">1</td><td char="." align="char">4.140</td><td align="left">Hexanal</td><td align="left">0.00a<sup>*</sup></td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.23 ± 0.12b</td><td align="left">0.00a</td></tr><tr><td align="left">2</td><td char="." align="char">5.733</td><td align="left">(E)-2-Hexenal</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.1 ± 0.09b</td><td align="left">0.00a</td></tr><tr><td align="left">3</td><td char="." align="char">5.746</td><td align="left">cis-3-Hexen-1-ol</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.27 ± 0.23b</td><td align="left">0.00a</td></tr><tr><td align="left">4</td><td char="." align="char">6.260</td><td align="left">cis-2-Hexen-1-ol</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.08 ± 0.15a</td><td align="left">0.00a</td></tr><tr><td align="left">5</td><td char="." align="char">6.345</td><td align="left">1-Pentanol</td><td align="left">0.41 ± 0.67a</td><td align="left">1.24 ± 1.01b</td><td align="left">0.11 ± 0.17a</td><td align="left">0.15 ± 0.17a</td><td align="left">0.00a</td></tr><tr><td align="left">6</td><td char="." align="char">6.741</td><td align="left">1-Hexanol</td><td align="left">0.18 ± 0.24b</td><td align="left">0.33 ± 0.39b</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td></tr><tr><td align="left">7</td><td char="." align="char">7.131</td><td align="left">2-Heptanone</td><td align="left">0.09 ± 0.13b</td><td align="left">0.02 ± 0.05a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td></tr><tr><td align="left">8</td><td char="." align="char">7.579</td><td align="left">2-Heptanol</td><td align="left">0.17 ± 0.18b</td><td align="left">0.15 ± 0.15ab</td><td align="left">0.00a</td><td align="left">0.10 ± 0.20ab</td><td align="left">0.00a</td></tr><tr><td align="left">9</td><td char="." align="char">9.989</td><td align="left">Benzaldehyde</td><td align="left">0.74 ± 0.87b</td><td align="left">0.17 ± 0.16a</td><td align="left">0.19 ± 0.04a</td><td align="left">0.41 ± 0.17ab</td><td align="left">0.18 ± 0.08a</td></tr><tr><td align="left">10</td><td char="." align="char">11.051</td><td align="left">1-Octen-3-ol</td><td align="left">0.34 ± 0.57ab</td><td align="left">0.96 ± 0.48c</td><td align="left">0.76 ± 0.78bc</td><td align="left">0.11 ± 0.24a</td><td align="left">0.03 ± 0.05a</td></tr><tr><td align="left">11</td><td char="." align="char">11.342</td><td align="left">6-Methyl-5-hepten-2-one</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.25 ± 0.14b</td><td align="left">0.00a</td><td align="left">0.00a</td></tr><tr><td align="left">12</td><td char="." align="char">11.599</td><td align="left">2-Pentyl-furan</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.81 ± 0.40bc</td><td align="left">1.37 ± 1.12c</td><td align="left">0.18 ± 0.10ab</td></tr><tr><td align="left">13</td><td char="." align="char">13.321</td><td align="left">Benzyl alcohol</td><td align="left">2.35 ± 1.43c</td><td align="left">3.45 ± 0.53d</td><td align="left">1.26 ± 0.87b</td><td align="left">0.39 ± 0.36a</td><td align="left">0.04 ± 0.06a</td></tr><tr><td align="left">14</td><td char="." align="char">13.590</td><td align="left">D-Limonene</td><td align="left">1.51 ± 1.60c</td><td align="left">1.2 ± 0.10bc</td><td align="left">0.32 ± 0.16a</td><td align="left">0.59 ± 0.58ab</td><td align="left">0.02 ± 0.05a</td></tr><tr><td align="left">15</td><td char="." align="char">14.049</td><td align="left">Phenylacetaldehyde</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.95 ± 0.51b</td><td align="left">0.04 ± 0.07a</td></tr><tr><td align="left">16</td><td char="." align="char">14.123</td><td align="left">1H-Pyrrole-2-carboxaldehyde</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.27 ± 0.29b</td><td align="left">0.19 ± 0.18ab</td></tr><tr><td align="left">17</td><td char="." align="char">14.413</td><td align="left">Ocimene</td><td align="left">0.57 ± 0.52b</td><td align="left">0.37 ± 0.25ab</td><td align="left">0.43 ± 0.2ab</td><td align="left">0.55 ± 0.77b</td><td align="left">0.00a</td></tr><tr><td align="left">18</td><td char="." align="char">15.427</td><td align="left">(E)-2-Octen-1-ol</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.35 ± 0.32b</td><td align="left">0.00a</td><td align="left">0.00a</td></tr><tr><td align="left">19</td><td char="." align="char">15.569</td><td align="left">Linalool oxide I</td><td align="left">3.77 ± 1.44a</td><td align="left">4.12 ± 0.83a</td><td align="left">0.98 ± 0.57b</td><td align="left">1.6 ± 0.85b</td><td align="left">1.11 ± 0.64b</td></tr><tr><td align="left">20</td><td char="." align="char">16.344</td><td align="left">Linalool oxide II</td><td align="left">4.61 ± 1.92a</td><td align="left">4.49 ± 1.15a</td><td align="left">2.10 ± 0.77b</td><td align="left">3.71 ± 1.77a</td><td align="left">2.17 ± 0.85b</td></tr><tr><td align="left">21</td><td char="." align="char">17.097</td><td align="left">Linalool</td><td align="left">19.97 ± 2.73a</td><td align="left">20.36 ± 1.54a</td><td align="left">13.23 ± 4.59a</td><td align="left">12.60 ± 14.78a</td><td align="left">0.80 ± 0.69b</td></tr><tr><td align="left">22</td><td char="." align="char">17.23</td><td align="left">3,7-Dimethyl-1,5,7-octatriene-3-ol</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">1.11 ± 1.42b</td><td align="left">0.00a</td></tr><tr><td align="left">23</td><td char="." align="char">17.513</td><td align="left">Phenylethyl alcohol</td><td align="left">1.86 ± 1.53a</td><td align="left">1.15 ± 1.10a</td><td align="left">0.41 ± 0.58a</td><td align="left">3.85 ± 6.04a</td><td align="left">0.36 ± 0.29a</td></tr><tr><td align="left">24</td><td char="." align="char">19.401</td><td align="left">1,2-dimethoxy benzene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">1.38 ± 0.41b</td></tr><tr><td align="left">25</td><td char="." align="char">20.266</td><td align="left">Linalool oxide III</td><td align="left">0.28 ± 0.29ab</td><td align="left">0.24 ± 0.11ab</td><td align="left">0.00a</td><td align="left">0.45 ± 0.45b</td><td align="left">0.52 ± 0.29b</td></tr><tr><td align="left">26</td><td char="." align="char">20.544</td><td align="left">Linalool oxide IV</td><td align="left">4.64 ± 2.52a</td><td align="left">1.35 ± 0.88b</td><td align="left">0.70 ± 0.43b</td><td align="left">1.88 ± 1.10b</td><td align="left">1.71 ± 0.98b</td></tr><tr><td align="left">27</td><td char="." align="char">20.703</td><td align="left">Naphthalene</td><td align="left">1.07 ± 1.02a</td><td align="left">0.96 ± 0.94a</td><td align="left">0.42 ± 0.22ab</td><td align="left">0.09 ± 0.13ab</td><td align="left">0.51 ± 0.90a</td></tr><tr><td align="left">28</td><td char="." align="char">21.302</td><td align="left">α-Terpineol</td><td align="left">2.14 ± 3.39ab</td><td align="left">1.65 ± 1.22ab</td><td align="left">2.82 ± 1.59b</td><td align="left">0.38 ± 0.37a</td><td align="left">1.60 ± 0.81ab</td></tr><tr><td align="left">29</td><td char="." align="char">21.439</td><td align="left">Methyl salicylate</td><td align="left">2.27 ± 1.84bc</td><td align="left">1.89 ± 1.28abc</td><td align="left">0.83 ± 1.14ab</td><td align="left">3.45 ± 2.00c</td><td align="left">0.41 ± 0.31a</td></tr><tr><td align="left">30</td><td char="." align="char">21.686</td><td align="left">Safranal</td><td align="left">0.94 ± 0.22a</td><td align="left">0.47 ± 0.37b</td><td align="left">0.34 ± 0.09b</td><td align="left">0.11 ± 0.09c</td><td align="left">0.15 ± 0.12c</td></tr><tr><td align="left">31</td><td char="." align="char">21.85</td><td align="left">Dodecane</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">2.39 ± 1.46b</td><td align="left">0.17 ± 0.32a</td><td align="left">0.05 ± 0.08a</td></tr><tr><td align="left">32</td><td char="." align="char">22.262</td><td align="left">Decanal</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.12 ± 0.07a</td><td align="left">0.27 ± 0.19b</td></tr><tr><td align="left">33</td><td char="." align="char">22.672</td><td align="left">β-Cyclocitral</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.63 ± 0.23ab</td><td align="left">0.29 ± 0.26b</td><td align="left">0.13 ± 0.10c</td></tr><tr><td align="left">34</td><td char="." align="char">23.135</td><td align="left">Nerol</td><td align="left">0.91 ± 1.00bc</td><td align="left">1.08 ± 1.06c</td><td align="left">0.32 ± 0.13a</td><td align="left">0.41 ± 0.21ab</td><td align="left">0.02 ± 0.06a</td></tr><tr><td align="left">35</td><td char="." align="char">23.824</td><td align="left">3,4-Dimethoxytoluene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.84 ± 0.79b</td></tr><tr><td align="left">36</td><td char="." align="char">24.467</td><td align="left">Geraniol</td><td align="left">2.82 ± 2.23a</td><td align="left">0.54 ± 0.40a</td><td align="left">1.68 ± 0.55a</td><td align="left">12.63 ± 7.26b</td><td align="left">0.47 ± 0.27a</td></tr><tr><td align="left">37</td><td char="." align="char">25.293</td><td align="left">2-Phenyl-2-butenal</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.38 ± 0.21b</td><td align="left">0.00a</td></tr><tr><td align="left">38</td><td char="." align="char">25.857</td><td align="left">2-Methyl-naphthalene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.36 ± 0.11c</td><td align="left">0.06 ± 0.07a</td><td align="left">0.22 ± 0.09b</td></tr><tr><td align="left">39</td><td char="." align="char">26.004</td><td align="left">Indole</td><td align="left">0.58 ± 0.47a</td><td align="left">0.76 ± 0.29a</td><td align="left">0.04 ± 0.09b</td><td align="left">0.02 ± 0.07b</td><td align="left">0.00b</td></tr><tr><td align="left">40</td><td char="." align="char">26.475</td><td align="left">Tridecane</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">6.23 ± 3.66b</td><td align="left">0.45 ± 1.07a</td><td align="left">0.00a</td></tr><tr><td align="left">41</td><td char="." align="char">26.578</td><td align="left">1-Methylnaphthalene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.06 ± 0.11a</td><td align="left">0.18 ± 0.12b</td></tr><tr><td align="left">42</td><td char="." align="char">27.027</td><td align="left">1,2,3-Trimethoxybenzene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.34 ± 0.27a</td><td align="left">0.00a</td><td align="left">14.41 ± 5.48b</td></tr><tr><td align="left">43</td><td char="." align="char">27.695</td><td align="left">4-Ethyl-1,4-dimethoxybenzene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">2.3 ± 1.31b</td></tr><tr><td align="left">44</td><td char="." align="char">28.624</td><td align="left">2,6-Dimethoxyphenol</td><td align="left">0.47 ± 0.15a</td><td align="left">0.79 ± 0.53b</td><td align="left">0.35 ± 0.23a</td><td align="left">0.24 ± 0.15a</td><td align="left">0.31 ± 0.2a</td></tr><tr><td align="left">45</td><td char="." align="char">29.840</td><td align="left">1,2,4-Trimethoxybenzene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">5.16 ± 2.85b</td></tr><tr><td align="left">46</td><td char="." align="char">30.093</td><td align="left">Damascenone</td><td align="left">0.58 ± 0.79a</td><td align="left">0.44 ± 0.25a</td><td align="left">0.00b</td><td align="left">0.21 ± 0.40ab</td><td align="left">0.00b</td></tr><tr><td align="left">47</td><td char="." align="char">30.239</td><td align="left">cis-3-Hexen-1-yl Hexanoate</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.46 ± 0.62b</td><td align="left">0.00a</td></tr><tr><td align="left">48</td><td char="." align="char">30.466</td><td align="left">Hexyl hexanoate</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.16 ± 0.14b</td><td align="left">0.00a</td></tr><tr><td align="left">49</td><td char="." align="char">30.705</td><td align="left">cis-Jasmone</td><td align="left">0.81 ± 0.99a</td><td align="left">0.57 ± 0.17a</td><td align="left">0.48 ± 0.38a</td><td align="left">0.63 ± 0.31a</td><td align="left">0.39 ± 0.18a</td></tr><tr><td align="left">50</td><td char="." align="char">30.842</td><td align="left">Tetradecane</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">1.10 ± 0.26c</td><td align="left">0.41 ± 0.10b</td><td align="left">0.41 ± 0.12b</td></tr><tr><td align="left">51</td><td char="." align="char">31.202</td><td align="left">1,3,5-Trimethoxybenzene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">3.40 ± 2.78b</td></tr><tr><td align="left">52</td><td char="." align="char">31.374</td><td align="left">α-Calacorene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.05 ± 0.10a</td><td align="left">0.20a ± 0.26</td><td align="left">0.8 ± 0.4b</td></tr><tr><td align="left">53</td><td char="." align="char">31.51</td><td align="left">β-Caryophyllene</td><td align="left">2.81 ± 3.22a</td><td align="left">3.08 ± 2.12a</td><td align="left">0.54 ± 0.56b</td><td align="left">0.06 ± 0.13b</td><td align="left">0.00b</td></tr><tr><td align="left">54</td><td char="." align="char">31.934</td><td align="left">α-Ionone</td><td align="left">0.81 ± 0.76ab</td><td align="left">0.84 ± 0.52ab</td><td align="left">1.35 ± 0.48b</td><td align="left">0.53 ± 0.41a</td><td align="left">0.82 ± 0.31ab</td></tr><tr><td align="left">55</td><td char="." align="char">32.294</td><td align="left">1,2-Benzopyrone</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.45 ± 0.13c</td><td align="left">0.25 ± 0.24b</td><td align="left">0.00a</td></tr><tr><td align="left">56</td><td char="." align="char">32.568</td><td align="left">4-(2,6,6-trimethyl-1-cyclohexen-1-yl)butan-2-one</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.03 ± 0.09a</td><td align="left">0.14 ± 0.15b</td></tr><tr><td align="left">57</td><td char="." align="char">32.645</td><td align="left">1-Methoxynaphthalene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.58 ± 0.29b</td></tr><tr><td align="left">58</td><td char="." align="char">32.855</td><td align="left">2-Methoxynaphthalene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.71 ± 0.25b</td></tr><tr><td align="left">59</td><td char="." align="char">32.979</td><td align="left">1,2,3,4-Tetramethoxybenzene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.93 ± 0.45b</td></tr><tr><td align="left">60</td><td char="." align="char">33.039</td><td align="left">Geranyl acetone</td><td align="left">1.02 ± 1.19a</td><td align="left">1.45 ± 0.71ab</td><td align="left">2.27 ± 0.82b</td><td align="left">1.12 ± 0.53a</td><td align="left">1.51 ± 0.68ab</td></tr><tr><td align="left">61</td><td char="." align="char">33.395</td><td align="left">β-Ionone</td><td align="left">1.40 ± 1.05b</td><td align="left">2.65 ± 1.57c</td><td align="left">0.07 ± 0.22a</td><td align="left">0.47 ± 0.42a</td><td align="left">0.00a</td></tr><tr><td align="left">62</td><td char="." align="char">34.388</td><td align="left">1-(2,6,6-trimethyl-3-cyclohexen-1-yl)-2-buten-1-one</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.15 ± 0.11a</td><td align="left">2.91 ± 2.30b</td></tr><tr><td align="left">63</td><td char="." align="char">34.358</td><td align="left">(E)-β-Farnesene</td><td align="left">3.93 ± 3.61a</td><td align="left">3.49 ± 3.15a</td><td align="left">5.34 ± 1.53a</td><td align="left">3.35 ± 2.57a</td><td align="left">2.67 ± 0.89a</td></tr><tr><td align="left">64</td><td char="." align="char">34.705</td><td align="left">Cocal</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.19 ± 0.19b</td><td align="left">0.00a</td></tr><tr><td align="left">65</td><td char="." align="char">34.97</td><td align="left">Pentadecane</td><td align="left">1.55 ± 1.78a</td><td align="left">1.75 ± 1.11a</td><td align="left">0.68 ± 0.21b</td><td align="left">0.52 ± 0.12b</td><td align="left">0.6 ± 0.16b</td></tr><tr><td align="left">66</td><td char="." align="char">35.012</td><td align="left">Methyl isoeugenol</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.41 ± 0.30b</td></tr><tr><td align="left">67</td><td char="." align="char">35.219</td><td align="left">Dibenzofuran</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.75 ± 0.64b</td><td align="left">0.31 ± 0.57ab</td><td align="left">0.53 ± 0.35ab</td></tr><tr><td align="left">68</td><td char="." align="char">35.313</td><td align="left">α-Farnesene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">1.13 ± 1.02b</td><td align="left">1.11 ± 0.67b</td><td align="left">0.32 ± 0.40a</td></tr><tr><td align="left">69</td><td char="." align="char">35.925</td><td align="left">Dihydroactinidiolide</td><td align="left">3.81 ± 0.82ab</td><td align="left">3.71 ± 0.29ab</td><td align="left">6.46 ± 1.12c</td><td align="left">2.50 ± 1.29a</td><td align="left">4.34 ± 1.48b</td></tr><tr><td align="left">70</td><td char="." align="char">37.467</td><td align="left">Nerolidol</td><td align="left">2.86 ± 3.28bc</td><td align="left">4.33 ± 1.56c</td><td align="left">0.29 ± 0.39a</td><td align="left">3.66 ± 1.73c</td><td align="left">1.17 ± 0.80ab</td></tr><tr><td align="left">71</td><td char="." align="char">37.688</td><td align="left">cis-3-Hexen-1-yl benzoate</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.66 ± 0.63b</td><td align="left">0.00a</td></tr><tr><td align="left">72</td><td char="." align="char">37.758</td><td align="left">Fluorene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">1.07 ± 0.42a</td><td align="left">0.14 ± 0.22b</td><td align="left">0.70 ± 0.31c</td></tr><tr><td align="left">73</td><td char="." align="char">38.79</td><td align="left">Cedrol</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.91 ± 0.41b</td><td align="left">0.64 ± 1.05ab</td><td align="left">1.15 ± 0.87b</td></tr><tr><td align="left">74</td><td char="." align="char">39.884</td><td align="left">Hexadecane</td><td align="left">0.53 ± 0.76a</td><td align="left">0.83 ± 0.27ab</td><td align="left">1.24 ± 0.65bc</td><td align="left">0.97 ± 0.23ab</td><td align="left">1.64 ± 0.66c</td></tr><tr><td align="left">75</td><td char="." align="char">40.845</td><td align="left">α-Cadinol</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">1.17 ± 0.20c</td><td align="left">0.51 ± 0.34b</td><td align="left">1.07 ± 0.14c</td></tr><tr><td align="left">76</td><td char="." align="char">40.897</td><td align="left">Methyl jasmonate</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.16 ± 0.36a</td><td align="left">0.00a</td></tr><tr><td align="left">77</td><td char="." align="char">41.051</td><td align="left">2,2′,5,5′-Tetra methylbiphenyl</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.22 ± 0.21a</td><td align="left">0.52 ± 0.39b</td></tr><tr><td align="left">78</td><td char="." align="char">42.584</td><td align="left">Heptadecane</td><td align="left">0.91 ± 0.95ab</td><td align="left">0.36 ± 0.28a</td><td align="left">1.19 ± 0.76ab</td><td align="left">0.70 ± 0.46ab</td><td align="left">1.44 ± 0.87b</td></tr><tr><td align="left">79</td><td char="." align="char">42.811</td><td align="left">2,6,10,14-Tetramethyl pentadecane</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">2.53 ± 1.44c</td><td align="left">1.23 ± 0.52b</td><td align="left">2.27 ± 1.28bc</td></tr><tr><td align="left">80</td><td char="." align="char">44.879</td><td align="left">Anthracene</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">1.17 ± 0.78b</td><td align="left">0.88 ± 1.28a</td><td align="left">1.39 ± 0.48b</td></tr><tr><td align="left">81</td><td char="." align="char">46.099</td><td align="left">Octadecane</td><td align="left">0.84 ± 0.67ab</td><td align="left">1.25 ± 0.20b</td><td align="left">0.79 ± 0.55ab</td><td align="left">0.36 ± 0.26a</td><td align="left">1.26 ± 1.01b</td></tr><tr><td align="left">82</td><td char="." align="char">46.425</td><td align="left">2,6,10,14-Tetramethyl hexadecane</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.61 ± 0.56a</td><td align="left">0.49 ± 0.41a</td><td align="left">1.44 ± 1.02b</td></tr><tr><td align="left">83</td><td char="." align="char">47.461</td><td align="left">Caffeine</td><td align="left">4.27 ± 2.15a</td><td align="left">3.59 ± 1.27a</td><td align="left">4.44 ± 3.62a</td><td align="left">4.50 ± 2.55a</td><td align="left">3.88 ± 2.29a</td></tr><tr><td align="left">84</td><td char="." align="char">47.645</td><td align="left">Phytone</td><td align="left">2.44 ± 1.51a</td><td align="left">2.38 ± 1.58a</td><td align="left">3.68 ± 1.47a</td><td align="left">3.18 ± 4.64a</td><td align="left">3.86 ± 1.83a</td></tr><tr><td align="left">85</td><td char="." align="char">50.021</td><td align="left">Farnesyl acetone</td><td align="left">1.49 ± 1.40a</td><td align="left">2.15 ± 0.20a</td><td align="left">3.04 ± 5.18a</td><td align="left">0.18 ± 0.23a</td><td align="left">0.49 ± 0.44a</td></tr><tr><td align="left">86</td><td char="." align="char">50.33</td><td align="left">Isophytol</td><td align="left">0.45 ± 0.47a</td><td align="left">0.31 ± 0.50a</td><td align="left">0.16 ± 0.28a</td><td align="left">0.17 ± 0.14a</td><td align="left">1.25 ± 0.60b</td></tr><tr><td align="left">87</td><td char="." align="char">51.006</td><td align="left">Hexadecanoic acid methyl ester</td><td align="left">3.08 ± 1.16a</td><td align="left">3.3 ± 0.80a</td><td align="left">0.53 ± 0.82c</td><td align="left">1.41 ± 0.89b</td><td align="left">0.54 ± 0.19c</td></tr><tr><td align="left">88</td><td char="." align="char">51.657</td><td align="left">Hexadecanoic acid</td><td align="left">2.25 ± 2.44a</td><td align="left">2.44 ± 2.09a</td><td align="left">2.36 ± 1.90a</td><td align="left">4.7 ± 5.02a</td><td align="left">9.05 ± 4.07b</td></tr><tr><td align="left">89</td><td char="." align="char">52.877</td><td align="left">Eicosane</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.00a</td><td align="left">0.24 ± 0.23b</td></tr><tr><td align="left">90</td><td char="." align="char">55.562</td><td align="left">Methyl linoleate</td><td align="left">0.52 ± 0.49a</td><td align="left">0.39 ± 0.25ab</td><td align="left">0.19 ± 0.23bc</td><td align="left">0.32 ± 0.28abc</td><td align="left">0.05 ± 0.11c</td></tr><tr><td align="left">91</td><td char="." align="char">55.759</td><td align="left">Methyl linolenate</td><td align="left">0.58 ± 0.55a</td><td align="left">0.92 ± 0.61a</td><td align="left">0.64 ± 0.63a</td><td align="left">0.49 ± 0.38a</td><td align="left">0.41 ± 0.31a</td></tr><tr><td align="left">92</td><td char="." align="char">56.192</td><td align="left">Phytol</td><td align="left">6.86 ± 2.32a</td><td align="left">6.67 ± 2.47a</td><td align="left">4.00 ± 3.40ab</td><td align="left">4.23 ± 3.53ab</td><td align="left">2.10 ± 1.86b</td></tr></tbody></table><table-wrap-foot><p><sup>*</sup>For each parameter, different letters within a row indicate difference between different types of tea with Duncan’s multiple range test (P < 0.05)</p></table-wrap-foot></table-wrap></p><p>CA can be used to show the natural groups that exist in a data set on the basis of the information provided by the measured variables (Chen et al. <xref ref-type="bibr" rid="CR3">2008</xref>; Wu et al. <xref ref-type="bibr" rid="CR26">2012</xref>). All percentage quantitative data of the 92 volatile compounds were used to calculate the CA model. The similarity or diversity between different samples (objects) is usually represented in a dendrogram for ease of explanation. The objects in the same group are similar to one another, and they are different from the objects in other groups. Figure <xref rid="Fig3" ref-type="fig">3</xref> shows that distinguishing Yunnan oolong tea (YO1–YO5) from Fujian oolong tea (FO1–FO5) is difficult; on the other hand, oolong tea (YO1–YO5 and FO1–FO5) and other types of teas (GT1–GT10, BT1–BT10, and PT1–PT10) were clearly different from one another. Oolong tea (YT1–YT5 and FT1–FT5) was clustered more closely with the black tea (BT1–BT10) because they are processed with a fermentation step, although oolong tea was semi-fermented. Finally, the following four main clusters were observed: the first one was composed of ten Pu-erh teas; the second one, ten green teas; the third one, ten oolong teas (five Yunnan oolong teas and five Fujian oolong teas but mixed together); and the fourth one, ten black teas.<fig id="Fig3"><label>Fig. 3</label><caption><p>Cluster analysis (CA) dendrogram of 40 tea samples</p></caption><graphic xlink:href="40064_2016_2229_Fig3_HTML" id="MO4"></graphic></fig></p><p>PCA is an effective way to discriminate between data observed (Ivosev et al. <xref ref-type="bibr" rid="CR9">2008</xref>). It also involves a linear transformation of multiple variables into a low-dimensional space that retains the maximum amount of information about the variables (Ma et al. <xref ref-type="bibr" rid="CR15">2013</xref>; Wu et al. <xref ref-type="bibr" rid="CR27">2013</xref>). Generally, the score plot provides a visual determination of similarity among the samples. PCA (Fig. <xref rid="Fig4" ref-type="fig">4</xref>) was conducted with the use of the same data as those used in the CA model. Figure <xref rid="Fig4" ref-type="fig">4</xref> shows that the score plot in the first two principal components (PC1 and PC2) represents 71.43 % of the total variability. The same figure shows that oolong teas (including five Yunnan oolong teas and five Fujian oolong teas) resembled one another closely and were clearly distinguished from the other types of teas in the PCA model; oolong tea was closer to black tea than to the other types of teas. These PCA results were mostly consistent with the results shown in Table <xref rid="Tab2" ref-type="table">2</xref>. The CA and PCA results also suggest that the volatile chemical compounds of the teas analyzed by fully automatic HS-SPME can be used for quality evaluation and control.<fig id="Fig4"><label>Fig. 4</label><caption><p>Principal component analysis (PCA) score derived from 92 volatile compounds of 40 tea samples: YO indicated by <italic>black color</italic> represents Yunnan oolong teas, FO indicated by <italic>red color</italic> represents Fujian oolong teas, GT indicated by <italic>blue color</italic> represents green teas, BT indicated by <italic>green color</italic> represents black teas, and PT indicated by <italic>yellow color</italic> represents Pu-erh teas</p></caption><graphic xlink:href="40064_2016_2229_Fig4_HTML" id="MO5"></graphic></fig></p><p>The contributions of all 92 aroma compounds to the PCA results are shown in Fig. <xref rid="Fig5" ref-type="fig">5</xref>. The variables that explained maximum variance in the data had high contributions and were considered important in discriminating samples between oolong tea and other types of teas. Benzylalcohol (V13), linalool oxides (V19, V20, V25, and V26), safranal (V30), indole (V39), <italic>β</italic>-ionone (V61), and hexadecanoic acid methyl ester (V87), which contributed to the fruity and flower-like aroma, had high positive values in oolong tea and were thought to enhance their aroma flavor (Kuo et al. <xref ref-type="bibr" rid="CR10">2011</xref>). These volatile compounds in oolong tea reached their highest levels during semi-fermentation (Wang et al. <xref ref-type="bibr" rid="CR22">2001</xref>). Fermentation was found to lead to the loss of grassy or green flavors and the formation of fruity and other fermented characters (Wang et al. <xref ref-type="bibr" rid="CR23">2008</xref>). Some nonalcoholic volatile compounds, such as benzylalcohol, safranal, and hexadecanoic acid methyl ester, were found to be transformed to glycosidically bound forms during fermentation in oolong tea (Guo et al. <xref ref-type="bibr" rid="CR7">1998</xref>; Yang et al. <xref ref-type="bibr" rid="CR29">2009</xref>). Geranyl pyrophosphate was the precursor for monoterpene alcohols, such as linalool. Some specific terpene synthases are involved in the biosynthesis of volatile monoterpene alcohols, which have been identified and validated in many plants (Creelman and Muleet <xref ref-type="bibr" rid="CR4">1995</xref>). Linalool oxide was synthesized from linalool by the possible synthesis pathway of monoterpenoids in tea. And the benzylalcohol in oolong tea was found to be related to the Ehrlich pathway that occurs in fermentation (Bode and Dong <xref ref-type="bibr" rid="CR2">2003</xref>). In addition, the tea-derived enzyme in oolong tea plants cleaves the 9,10 (9′10′)-double bonds of arotenoids and long-chained apocarotenoids to yield <italic>β</italic>-ionone (Felfe et al. <xref ref-type="bibr" rid="CR5">2011</xref>). This result was also mostly consistent with the typical aroma compounds of oolong tea shown in Table <xref rid="Tab2" ref-type="table">2</xref>. Because the volatile compounds were influenced by biological and chemical transformations during cultivation and processing, we can conclude that these typical aroma compounds, which made oolong tea different from other types of teas, were largely influenced by the semi-fermentation step. Prior to this step, the bruising step breaks the cell membrane and eventually facilitates the mixture of precursors with biological enzymes. Hereafter, the most significant changes are the rapid conversions and transformations of the precursors to benzylalcohol, indole, safranal, linalool oxides, <italic>β</italic>-ionone, and hexadecanoic acid methyl ester, mostly by enzymatic catalysis and chemical processes. Therefore, fermentation intensity influences the quantity of most tea volatiles during the manufacturing process of green, oolong and black tea; and because of the distinctive processes of oolong tea, its aroma characteristics are different from the unfermented green or fully fermented black tea (Baldermann et al. <xref ref-type="bibr" rid="CR1">2014</xref>).<fig id="Fig5"><label>Fig. 5</label><caption><p>Coefficient plot related to the contribution of 92 volatile compounds to the principal component analysis (PCA) results. The number (No.) of volatile compounds are consistent with that of Table <xref rid="Tab2" ref-type="table">2</xref></p></caption><graphic xlink:href="40064_2016_2229_Fig5_HTML" id="MO6"></graphic></fig></p><p>In summary, our results suggested that the aroma characteristics of oolong tea, which are either Yunnan oolong tea (<italic>Camellia sinensis</italic> var. <italic>assamica</italic>) or Fujian oolong tea (<italic>Camellia</italic><italic>sinensis</italic> var. <italic>sinensis</italic>), were mostly consistent compared with those of the other three types of teas (green, black, and Pu-erh tea). These findings indicated that although the raw materials, cultivation measures used and the environment factors involved in tea production influence water-soluble and aroma components, processing technology plays a crucial role in the formation of tea aroma. Further investigation will focus on the influence of other factors (geographic characteristics, cultivars, etc.), particularly each processing step, on final aroma characteristics in the proposal of guidelines for the quality control of tea products.</p></sec></sec><sec id="Sec16"><title>Conclusion</title><p>This work reported for the first time that the same types of teas made from different tea tree leaves but the same processing technology showed similar aroma flavor. Our results demonstrated that the sensory evaluation and main water-soluble components, i.e., caffeine, catechins, and total polysaccharides, of Yunnan oolong tea were different from those of Fujian oolong tea, but no significant difference was observed between their aroma characteristics, as shown in the PCA and CA analyses. The PCA results showed that benzylalcohol, indole, safranal, linalool oxides, <italic>β</italic>-ionone, and hexadecanoic acid methyl ester strongly contributed to the aroma flavor of oolong tea compared to the case of the green, black, and Pu-erh teas. Although the raw materials, cultivation measures used and the environment factors involved in tea production influence water-soluble and aroma components among different kinds of teas, processing technique for oolong teas from different tea trees, especially the semi-fermentation process, is the main driver of tea aroma characteristics.</p></sec></body><back><fn-group><fn><p>Chen Wang and Shidong Lv contribute equally to this work should be regarded as co-first authors</p></fn></fn-group><ack><title>Authors’ contributions</title><p>CW and SL conceived and designed the experiments; CW, YW, XG, JL, WZ performed the experiments; WC and SL analyzed the data; QM contributed reagents/materials/analysis tools; CW and SL wrote the paper. All authors read and approved the final manuscript.</p><sec id="FPar1"><title>Acknowledgements</title><p>We are grateful to Donghua Jiang and Zhenggang Luo (National Centre for Pu-erh Tea Production Quality Supervision and Inspection, Pu-erh, Yunnan, China) for their technical assistance with samples identification. This work was supported by the National Natural Science Foundation of China (No. 31460228) and scientific research funds in Yunnan province Department of Education (No. 2014Y089).</p></sec><sec id="FPar2"><title>Competing interests</title><p>The authors declare that they have no competing interests.</p></sec></ack><ref-list id="Bib1"><title>References</title><ref id="CR1"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baldermann</surname><given-names>S</given-names></name><name><surname>Yang</surname><given-names>Z</given-names></name><name><surname>Katsuno</surname><given-names>T</given-names></name><name><surname>Tu</surname><given-names>VA</given-names></name><name><surname>Mase</surname><given-names>N</given-names></name><name><surname>Nakamura</surname><given-names>Y</given-names></name><name><surname>Watanabe</surname><given-names>N</given-names></name></person-group><article-title>Discrimination of green, oolong, and black 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