Nucellain, a Barley Homolog of the Dicot Vacuolar-Processing Protease, Is Localized in Nucellar Cell Walls1
Identifieur interne : 000541 ( Pmc/Corpus ); précédent : 000540; suivant : 000542Nucellain, a Barley Homolog of the Dicot Vacuolar-Processing Protease, Is Localized in Nucellar Cell Walls1
Auteurs : Casper Linnestad ; Danny N. P. Doan ; Roy C. Brown ; Betty E. Lemmon ; David J. Meyer ; Rudolf Jung ; Odd-Arne OlsenSource :
- Plant Physiology [ 0032-0889 ] ; 1998.
Abstract
The nucellus is a complex maternal
grain tissue that embeds and feeds the developing cereal endosperm and
embryo. Differential screening of a barley (
Url:
PubMed: 9847091
PubMed Central: 34733
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PMC:34733Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Nucellain, a Barley Homolog of the Dicot
Vacuolar-Processing
Protease, Is Localized in
Nucellar Cell Walls<xref ref-type="fn" rid="FN1">1</xref>
</title>
<author><name sortKey="Linnestad, Casper" sort="Linnestad, Casper" uniqKey="Linnestad C" first="Casper" last="Linnestad">Casper Linnestad</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242c0">Plant Molecular Biology Laboratory, Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5051, N-1432 Aas, Norway (C.L., D.N.P.D., O.-A.O.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Doan, Danny N P" sort="Doan, Danny N P" uniqKey="Doan D" first="Danny N. P." last="Doan">Danny N. P. Doan</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242c0">Plant Molecular Biology Laboratory, Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5051, N-1432 Aas, Norway (C.L., D.N.P.D., O.-A.O.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Brown, Roy C" sort="Brown, Roy C" uniqKey="Brown R" first="Roy C." last="Brown">Roy C. Brown</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242e4">Department of Biology, The University of Southwestern Louisiana, P.O. Box 42451, Lafayette, Louisiana 70504–2451 (R.C.B., B.E.L.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lemmon, Betty E" sort="Lemmon, Betty E" uniqKey="Lemmon B" first="Betty E." last="Lemmon">Betty E. Lemmon</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242e4">Department of Biology, The University of Southwestern Louisiana, P.O. Box 42451, Lafayette, Louisiana 70504–2451 (R.C.B., B.E.L.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Meyer, David J" sort="Meyer, David J" uniqKey="Meyer D" first="David J." last="Meyer">David J. Meyer</name>
<affiliation><nlm:aff id="N0xac90e30N0xad24308">Pioneer Hi-Bred International, 7300 N.W. 62nd Avenue, P.O. Box 1004, Johnston, Iowa 50131–1004 (D.J.M., R.J.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jung, Rudolf" sort="Jung, Rudolf" uniqKey="Jung R" first="Rudolf" last="Jung">Rudolf Jung</name>
<affiliation><nlm:aff id="N0xac90e30N0xad24308">Pioneer Hi-Bred International, 7300 N.W. 62nd Avenue, P.O. Box 1004, Johnston, Iowa 50131–1004 (D.J.M., R.J.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Olsen, Odd Arne" sort="Olsen, Odd Arne" uniqKey="Olsen O" first="Odd-Arne" last="Olsen">Odd-Arne Olsen</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242c0">Plant Molecular Biology Laboratory, Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5051, N-1432 Aas, Norway (C.L., D.N.P.D., O.-A.O.)</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">9847091</idno>
<idno type="pmc">34733</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC34733</idno>
<idno type="RBID">PMC:34733</idno>
<date when="1998">1998</date>
<idno type="wicri:Area/Pmc/Corpus">000541</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Nucellain, a Barley Homolog of the Dicot
Vacuolar-Processing
Protease, Is Localized in
Nucellar Cell Walls<xref ref-type="fn" rid="FN1">1</xref>
</title>
<author><name sortKey="Linnestad, Casper" sort="Linnestad, Casper" uniqKey="Linnestad C" first="Casper" last="Linnestad">Casper Linnestad</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242c0">Plant Molecular Biology Laboratory, Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5051, N-1432 Aas, Norway (C.L., D.N.P.D., O.-A.O.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Doan, Danny N P" sort="Doan, Danny N P" uniqKey="Doan D" first="Danny N. P." last="Doan">Danny N. P. Doan</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242c0">Plant Molecular Biology Laboratory, Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5051, N-1432 Aas, Norway (C.L., D.N.P.D., O.-A.O.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Brown, Roy C" sort="Brown, Roy C" uniqKey="Brown R" first="Roy C." last="Brown">Roy C. Brown</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242e4">Department of Biology, The University of Southwestern Louisiana, P.O. Box 42451, Lafayette, Louisiana 70504–2451 (R.C.B., B.E.L.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lemmon, Betty E" sort="Lemmon, Betty E" uniqKey="Lemmon B" first="Betty E." last="Lemmon">Betty E. Lemmon</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242e4">Department of Biology, The University of Southwestern Louisiana, P.O. Box 42451, Lafayette, Louisiana 70504–2451 (R.C.B., B.E.L.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Meyer, David J" sort="Meyer, David J" uniqKey="Meyer D" first="David J." last="Meyer">David J. Meyer</name>
<affiliation><nlm:aff id="N0xac90e30N0xad24308">Pioneer Hi-Bred International, 7300 N.W. 62nd Avenue, P.O. Box 1004, Johnston, Iowa 50131–1004 (D.J.M., R.J.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jung, Rudolf" sort="Jung, Rudolf" uniqKey="Jung R" first="Rudolf" last="Jung">Rudolf Jung</name>
<affiliation><nlm:aff id="N0xac90e30N0xad24308">Pioneer Hi-Bred International, 7300 N.W. 62nd Avenue, P.O. Box 1004, Johnston, Iowa 50131–1004 (D.J.M., R.J.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Olsen, Odd Arne" sort="Olsen, Odd Arne" uniqKey="Olsen O" first="Odd-Arne" last="Olsen">Odd-Arne Olsen</name>
<affiliation><nlm:aff id="N0xac90e30N0xad242c0">Plant Molecular Biology Laboratory, Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5051, N-1432 Aas, Norway (C.L., D.N.P.D., O.-A.O.)</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Plant Physiology</title>
<idno type="ISSN">0032-0889</idno>
<idno type="eISSN">1532-2548</idno>
<imprint><date when="1998">1998</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The nucellus is a complex maternal
grain tissue that embeds and feeds the developing cereal endosperm and
embryo. Differential screening of a barley (<italic>Hordeum
vulgare</italic>
) cDNA library from 5-d-old ovaries resulted in the
isolation of two cDNA clones encoding nucellus-specific homologs of the
vacuolar-processing enzyme of castor bean (<italic>Ricinus
communis</italic>
). Based on the sequence of these barley clones, which
are called nucellains, a homolog from developing corn (<italic>Zea
mays</italic>
) grains was also identified. In dicots the
vacuolar-processing enzyme is believed to be involved in the processing
of vacuolar storage proteins. RNA-blot and in situ-hybridization
analyses detected nucellain transcripts in autolysing nucellus
parenchyma cells, in the nucellar projection, and in the nucellar
epidermis. No nucellain transcripts were detected in the highly
vacuolate endosperm or in the other maternal tissues of developing
grains such as the testa or the pericarp. Using an antibody raised
against castor bean vacuolar-processing protease, a single polypeptide
was recognized in protein extracts from barley grains.
Immunogold-labeling experiments with this antibody localized the
nucellain epitope not in the vacuoles, but in the cell walls of all
nucellar cell types. We propose that nucellain plays a role in
processing and/or turnover of cell wall proteins in developing cereal
grains.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id>
<journal-id journal-id-type="publisher-id">PLANT PHYSIOLOGY</journal-id>
<journal-title>Plant Physiology</journal-title>
<issn pub-type="ppub">0032-0889</issn>
<issn pub-type="epub">1532-2548</issn>
<publisher><publisher-name>American Society of Plant Physiologists</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">9847091</article-id>
<article-id pub-id-type="pmc">34733</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
<subj-group><subject>Molecular Genetics and Genomics</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Nucellain, a Barley Homolog of the Dicot
Vacuolar-Processing
Protease, Is Localized in
Nucellar Cell Walls<xref ref-type="fn" rid="FN1">1</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Linnestad</surname>
<given-names>Casper</given-names>
</name>
<xref ref-type="aff" rid="N0xac90e30N0xad242c0"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Doan</surname>
<given-names>Danny N.P.</given-names>
</name>
<xref ref-type="aff" rid="N0xac90e30N0xad242c0"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Brown</surname>
<given-names>Roy C.</given-names>
</name>
<xref ref-type="aff" rid="N0xac90e30N0xad242e4"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lemmon</surname>
<given-names>Betty E.</given-names>
</name>
<xref ref-type="aff" rid="N0xac90e30N0xad242e4"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Meyer</surname>
<given-names>David J.</given-names>
</name>
<xref ref-type="aff" rid="N0xac90e30N0xad24308"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jung</surname>
<given-names>Rudolf</given-names>
</name>
<xref ref-type="aff" rid="N0xac90e30N0xad24308"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Olsen</surname>
<given-names>Odd-Arne</given-names>
</name>
<xref ref-type="author-notes" rid="FN150">*</xref>
<xref ref-type="aff" rid="N0xac90e30N0xad242c0"></xref>
</contrib>
</contrib-group>
<aff id="N0xac90e30N0xad242c0">Plant Molecular Biology Laboratory, Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5051, N-1432 Aas, Norway (C.L., D.N.P.D., O.-A.O.)</aff>
<aff id="N0xac90e30N0xad242e4">Department of Biology, The University of Southwestern Louisiana, P.O. Box 42451, Lafayette, Louisiana 70504–2451 (R.C.B., B.E.L.)</aff>
<aff id="N0xac90e30N0xad24308">Pioneer Hi-Bred International, 7300 N.W. 62nd Avenue, P.O. Box 1004, Johnston, Iowa 50131–1004 (D.J.M., R.J.)</aff>
<author-notes><fn id="FN150"><label>*</label>
<p>Corresponding author; e-mail <email>odd-arne.olsen@ibf.nlh.no</email>
; fax
47–64941465.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>12</month>
<year>1998</year>
</pub-date>
<volume>118</volume>
<issue>4</issue>
<fpage>1169</fpage>
<lpage>1180</lpage>
<history><date date-type="received"><day>8</day>
<month>5</month>
<year>1998</year>
</date>
<date date-type="accepted"><day>3</day>
<month>9</month>
<year>1998</year>
</date>
</history>
<copyright-year>1998</copyright-year>
<abstract><p>The nucellus is a complex maternal
grain tissue that embeds and feeds the developing cereal endosperm and
embryo. Differential screening of a barley (<italic>Hordeum
vulgare</italic>
) cDNA library from 5-d-old ovaries resulted in the
isolation of two cDNA clones encoding nucellus-specific homologs of the
vacuolar-processing enzyme of castor bean (<italic>Ricinus
communis</italic>
). Based on the sequence of these barley clones, which
are called nucellains, a homolog from developing corn (<italic>Zea
mays</italic>
) grains was also identified. In dicots the
vacuolar-processing enzyme is believed to be involved in the processing
of vacuolar storage proteins. RNA-blot and in situ-hybridization
analyses detected nucellain transcripts in autolysing nucellus
parenchyma cells, in the nucellar projection, and in the nucellar
epidermis. No nucellain transcripts were detected in the highly
vacuolate endosperm or in the other maternal tissues of developing
grains such as the testa or the pericarp. Using an antibody raised
against castor bean vacuolar-processing protease, a single polypeptide
was recognized in protein extracts from barley grains.
Immunogold-labeling experiments with this antibody localized the
nucellain epitope not in the vacuoles, but in the cell walls of all
nucellar cell types. We propose that nucellain plays a role in
processing and/or turnover of cell wall proteins in developing cereal
grains.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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