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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Altering the Expression of the Chlorophyllase Gene <italic>ATHCOR1</italic> in Transgenic Arabidopsis Caused Changes in the Chlorophyll-to-Chlorophyllide Ratio<xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Benedetti, Celso Eduardo" sort="Benedetti, Celso Eduardo" uniqKey="Benedetti C" first="Celso Eduardo" last="Benedetti">Celso Eduardo Benedetti</name><affiliation><nlm:aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</nlm:aff></affiliation></author><author><name sortKey="Arruda, Paulo" sort="Arruda, Paulo" uniqKey="Arruda P" first="Paulo" last="Arruda">Paulo Arruda</name><affiliation><nlm:aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">11950974</idno><idno type="pmc">154253</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154253</idno><idno type="RBID">PMC:154253</idno><idno type="doi">10.1104/pp.010813</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">000538</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Altering the Expression of the Chlorophyllase Gene <italic>ATHCOR1</italic> in Transgenic Arabidopsis Caused Changes in the Chlorophyll-to-Chlorophyllide Ratio<xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Benedetti, Celso Eduardo" sort="Benedetti, Celso Eduardo" uniqKey="Benedetti C" first="Celso Eduardo" last="Benedetti">Celso Eduardo Benedetti</name><affiliation><nlm:aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</nlm:aff></affiliation></author><author><name sortKey="Arruda, Paulo" sort="Arruda, Paulo" uniqKey="Arruda P" first="Paulo" last="Arruda">Paulo Arruda</name><affiliation><nlm:aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</nlm:aff></affiliation></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The Arabidopsis gene <italic>ATHCOR1</italic>, which encodes the CORI1 (coronatine-induced) protein, was expressed in bacterial cells. Soluble recombinant CORI1 was purified and shown to possess chlorophyllase (Chlase) activity in vitro. To determine its activity in vivo, wild-type Arabidopsis and <italic>coi1</italic> mutant, which lacks <italic>ATHCOR1</italic> transcripts, were transformed with sense and antisense forms of the gene. Wild-type and <italic>coi1</italic> plants overexpressing <italic>ATHCOR1</italic> showed increased contents of chlorophyllide (Chlide) without a substantial change in the total amount of the extractable chlorophyll (Chl). These plants presented high Chlide to Chl ratios in leaves, whereas antisense plants and nontransformed <italic>coi1</italic> mutant showed undetectable <italic>ATHCOR1</italic> mRNA and significantly lower Chlide to Chl ratios, relative to wild-type control. Overexpression of <italic>ATHCOR1</italic> caused an increased breakdown of Chl <italic>a</italic>, as revealed by the Chlide <italic>a</italic> to <italic>b</italic> ratio, which was significantly higher in sense than wild-type, <italic>coi1</italic> mutant, and antisense plants. This preferential activity of CORI1 toward Chl <italic>a</italic> was further supported by in vitro analyses using the purified protein. Increased Chlase activity was detected in developing flowers, which correlated to the constitutive expression of <italic>ATHCOR1</italic> in this organ. Flowers of the antisense plant showed reduced Chlide to Chl ratio, suggesting a role of CORI1 in Chl breakdown during flower senescence. The results show that <italic>ATHCOR1</italic> has Chlase activity in vivo, however, because <italic>coi1</italic> flowers have no detectable <italic>ATHCOR1</italic> mRNA and present Chlide to Chl ratios comparable with the wild type, an additional Chlase is likely to be active in Arabidopsis. In accordance, transcripts of a second Arabidopsis Chlase gene, <italic>AtCLH2</italic>, were detected in both normal and mutant flowers.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="publisher-id">PLANT PHYSIOL</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Physiologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">11950974</article-id><article-id pub-id-type="pmc">154253</article-id><article-id pub-id-type="publisher-id">7177</article-id><article-id pub-id-type="doi">10.1104/pp.010813</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject><subj-group><subject>Biochemical Processes and Macromolecular Structures</subject></subj-group></subj-group></article-categories><title-group><article-title>Altering the Expression of the Chlorophyllase Gene <italic>ATHCOR1</italic> in Transgenic Arabidopsis Caused Changes in the Chlorophyll-to-Chlorophyllide Ratio<xref ref-type="fn" rid="FN1">1</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Benedetti</surname><given-names>Celso Eduardo</given-names></name><xref ref-type="author-notes" rid="FN150">*</xref><xref ref-type="aff" rid="N0x9e06d20N0x9ebfb30"></xref></contrib><contrib contrib-type="author"><name><surname>Arruda</surname><given-names>Paulo</given-names></name><xref ref-type="aff" rid="N0x9e06d20N0x9ebfb30"></xref></contrib></contrib-group><aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</aff><author-notes><fn id="FN150"><label>*</label><p>Corresponding author; e-mail <email>celso@lnls.br</email>; fax 5519–32877110.</p></fn></author-notes><pub-date pub-type="ppub"><month>4</month><year>2002</year></pub-date><volume>128</volume><issue>4</issue><fpage>1255</fpage><lpage>1263</lpage><history><date date-type="received"><day>4</day><month>9</month><year>2001</year></date><date date-type="rev-recd"><day>31</day><month>10</month><year>2001</year></date><date date-type="accepted"><day>4</day><month>1</month><year>2002</year></date></history><copyright-statement>Copyright © 2002, American Society of Plant Physiologists</copyright-statement><copyright-year>2002</copyright-year><abstract><p>The Arabidopsis gene <italic>ATHCOR1</italic>, which encodes the CORI1 (coronatine-induced) protein, was expressed in bacterial cells. Soluble recombinant CORI1 was purified and shown to possess chlorophyllase (Chlase) activity in vitro. To determine its activity in vivo, wild-type Arabidopsis and <italic>coi1</italic> mutant, which lacks <italic>ATHCOR1</italic> transcripts, were transformed with sense and antisense forms of the gene. Wild-type and <italic>coi1</italic> plants overexpressing <italic>ATHCOR1</italic> showed increased contents of chlorophyllide (Chlide) without a substantial change in the total amount of the extractable chlorophyll (Chl). These plants presented high Chlide to Chl ratios in leaves, whereas antisense plants and nontransformed <italic>coi1</italic> mutant showed undetectable <italic>ATHCOR1</italic> mRNA and significantly lower Chlide to Chl ratios, relative to wild-type control. Overexpression of <italic>ATHCOR1</italic> caused an increased breakdown of Chl <italic>a</italic>, as revealed by the Chlide <italic>a</italic> to <italic>b</italic> ratio, which was significantly higher in sense than wild-type, <italic>coi1</italic> mutant, and antisense plants. This preferential activity of CORI1 toward Chl <italic>a</italic> was further supported by in vitro analyses using the purified protein. Increased Chlase activity was detected in developing flowers, which correlated to the constitutive expression of <italic>ATHCOR1</italic> in this organ. Flowers of the antisense plant showed reduced Chlide to Chl ratio, suggesting a role of CORI1 in Chl breakdown during flower senescence. The results show that <italic>ATHCOR1</italic> has Chlase activity in vivo, however, because <italic>coi1</italic> flowers have no detectable <italic>ATHCOR1</italic> mRNA and present Chlide to Chl ratios comparable with the wild type, an additional Chlase is likely to be active in Arabidopsis. In accordance, transcripts of a second Arabidopsis Chlase gene, <italic>AtCLH2</italic>, were detected in both normal and mutant flowers.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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